Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf

Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

... is under the direct control of kinases and phosphatases, and the indirect control of phosphorylation through the regula-tion of other apoptotic proteins. Furthermore, manykinases and phosphatases ... and increases the release of cytochrome c from mitochon-dria into the cytosol through inactivation with Bcl-xL and Bcl-2 [69]. Inactivated Bax and Bad bind 14-3-3, the phosphoserine ⁄ threonine binding proteins ... pro-apoptotic proteins exist in mitochondria and are released during apoptosis. These includeapoptosis-inducing factor (AIF) and EndoG, whichare located in the mitochondrial intermembrane spacedue to the...

Tài liệu Báo cáo khoa học: From heart to mind The urotensin II system and its evolving neurophysiological role ppt

... character-ized by an increase in cell size and protein content in the absence of cell proliferation. In the CNS, indirect evidence exist that UII also activates ERK and Rho -kinase pathways. In rat, cen-trally ... activation of the channels viaproteinase kinase A dependent phosphorylation and no involvement of PKC in the process.While the UII receptor was initially considered toonly couple Gaqproteins, the ... However, the binding sites matched the projection areas of the LDT–PPT complex leading to the hypothesis that the binding sites might represent presynaptic neuro-nal terminals. Because cholinergic...

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... the protein protein interactionbetween v-KIND and MAP2 (i.e. the MAP2-bindingcore module in v-KIND and the v-KIND-binding coremodule in MAP2) across a range of amino acid resi-dues, and provided ... (RasGEF) very-KIND (v-KIND, or kinase noncatalytic C-lobedomain containing 1) [9]. The KIND domain in theseproteins is localized to the N-terminal region, and theirspeciﬁc functional domains are ... February2011)doi:10.1111/j.1742-4658.2011.08085.x The kinase noncatalytic C-lobe domain (KIND) is a putative protein protein interaction module. Four KIND-containing proteins, Spir-2 (actin-nuclearfactor), PTPN13 (protein tyrosine phosphatase),...

Tài liệu Báo cáo khoa học: Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast pdf

... aprotinin, leupeptin and pepstatin, twice in kinase buffer, and used for in vitro kinase assays. In vitro kinase assaysRecombinant human protein kinase CK2 holoenzyme(0.5 U, corresponding to ... progression through the G1phase [11].Keywords atypical protein kinase; casein kinase 2substrate; cell-cycle phase-dependent degradation; protein protein interactions;Rio1 protein kinase 21CorrespondenceM. ... Authors Journal compilation ª 2007 FEBS 4667 Protein kinase CK2 activates the atypical Rio1p kinase and promotes its cell-cycle phase-dependent degradation in yeast Michaela Angermayr1,*, Elisabeth...

Tài liệu Báo cáo khoa học: An alternative transcript from the death-associated protein kinase 1 locus encoding a small protein selectively mediates membrane blebbing pdf

... protein kinase 1 (DAPK-1) is aCa2+⁄ calmodulin-regulated serine ⁄ threonine kinase composed of multiple functional domains, including a kinase domain, a calmodulin-binding domain, eightankyrin ... Furthermore,microtubule-associated protein 1B interaction with the kinase domain of DAPK-1 stimulates membrane bleb-bing and autophagy [12]. The roles of its other func-tional domains in its regulatory effects are beingcharacterized. ... activities. The DAPK-1 kinase domainis required to mediate cytoskeleton remodeling byphosphorylating myosin light chain 2 [8], inhibiting cellmigration [9] and inducing membrane blebbing [10].The...

Tài liệu Báo cáo khoa học: Salt-induced formation of the A-state of ferricytochrome c – effect of the anion charge on protein structure docx

... understanding of the folding mechanism(s) and the factors affecting protein stabilization, in this article we extend our knowledgeon anion protein interactions by determining the effecton the protein ... and LS stand for high- and low-spin, and IHH and IHMindicate the bis-histidine and the His-Metcoordinated intermediates) we observe that the sulfateinduces a similar kinetic pathway to the ... region,are inverted, whereas the CT band is weakened and red-shifted. This indicates that the mutations introducedinto the protein bring about changes at the level of the heme coordination, and suggests...

Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt

... proposed to inject the acyl chain into the sub-strate-binding pocket [32]. If a similar mechanism isinvolved in the KAS I reaction then an intermediateACP-acyl–KAS I crystal complex remains to ... mutants all pointto the orientation of H333 and its hydrogen bonddonor properties as being the cornerstone of obtaining and ⁄ or retaining residual decarboxylase activity. The emerging picture ... H298E and H298Q proteins in 30 minassays. The amount of labeled substrate present at the start of the reactions is shown in the ﬁrst lane. C is a standard consisting of malo-nyl-ACP and acetyl-ACP...

Tài liệu Báo cáo khoa học: Speciﬁc interaction between the classical swine fever virus NS5B protein and the viral genome pdf

... dRpassays, and examined the in uence of d ifferent cis-actingelements on binding of NS5B protein and RNA synthesis in vitro.Materials and methodsExpression and puriﬁcation of NS5B proteins The recombinant ... plus-RNAsynthesis might be present in the minus-RNA, as well as i n the plus-RNA. To investigate the binding activity of the CSFV NS5B protein on the minus-RNA, the competitiveEMSA was performed with the ... tobinding of the CSFV NS5B protein. The minus-strand3¢ UTR with deletion of the 21 nucleotide fragment at the terminal did not interact with the N S5B protein at all.Characterization of the cis-acting...

Tài liệu Báo cáo khoa học: Co-operative effect of the isoforms of type III antifreeze protein expressed in Notched-ﬁn eelpout, Zoarces elongatus Kner ppt

... 94%, and the former shares98% and 85% identities with the N-terminal and C-terminal domains of RD3, respectively (for RD2, the corresponding identities are 94% and 77%,respectively). For RD1 and ... fragments of the peak cleaved by TPCK tryp-sin (T1–T5). By comparing the cleaved sequences witheach other, the N1 and T1 fragments were assigned to the N-terminal sequence, and N5 and T5 to the C-ter-minal ... and nfeAFP1–6 were categorized into the SPgroup and nfeAFP7–13 into the QAE group. For clar-ity, Gly1 of the SP group is deﬁned as the 2nd residue in the present study. We further divided the...

Tài liệu Báo cáo khoa học: Protein farnesyltransferase inhibitors interfere with farnesyl diphosphate binding by rubber transferase pdf

... lMchaetomellicacid A (2) and not at all by 180 lMcompound 4. In bothcases, lengthening the hydrophobic surface of the analogadded additional interactions, which increased the bindingafﬁnity of the inhibitor ... for the initiator-binding site. The difference in the inhibition of H. brasiliensis and P.argent-atum rubber transferases by chaetomellic acid A indicatedthat there may be differences in the ... surface lining the catalyticcavity [15]. Alternatively, the difference in length of the inhibitors allows 2 to interact with the polyisoprenemolecules within the rubber particle. In both cases, the additional...

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