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Tài liệu Báo cáo khoa học: Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450 1) determined by SAIL-NMR docx

Tài liệu Báo cáo khoa học: Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR docx

Tài liệu Báo cáo khoa học: Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR docx

... with unknown structure. We report here the three-dimensional structure of the At3g16450.1 protein from Arabidopsis, which consists of two tandem repeats. Because the size of the protein is larger ... for the sugar structure, although none of the various sugars tested exhibited a strong affinity.DiscussionIn this study, we determined the solution structure of the 32 kDa At3g16450.1 protein from ... 3068–3077. SAIL-NMR structure of a myrosinase-binding protein M. Takeda et al.5884 FEBS Journal 275 (2008) 5873–5884 ª 2008 The Authors Journal compilation ª 2008 FEBS Structure of the putative 32 kDa...
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Tài liệu Báo cáo khoa học: Structure of RNase Sa2 complexes with mononucleotides – new aspects of catalytic reaction and substrate recognition pptx

Tài liệu Báo cáo khoa học: Structure of RNase Sa2 complexes with mononucleotides – new aspects of catalytic reaction and substrate recognition pptx

... constraint of 4.5 A˚between the geometrical center of the main chain nitrogen of Arg42 andO6 of the leading guanine. Accuracy of the docking wasassessed on the basis of scoring values calculated by ... the positions of the ribose and base of the second nucleotide. In all cases, the base of the secondnucleotide interacted with the Asp66–Thr69 loop andwith His86 (Fig. 4B). The majority of the ... Arg67–Thr69. In the second cluster, the riboseand the base of the third nucleotide are close to Tyr32,Asn33, and Arg34, which corresponds to the position of the bound glucose. The presence of the third...
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Tài liệu Báo cáo khoa học: Structure of peptidase T from Salmonella typhimurium doc

Tài liệu Báo cáo khoa học: Structure of peptidase T from Salmonella typhimurium doc

... CG2, the lack of clear homology outside these regions, and the unique tripeptidase speci®city of peptidase T, suggested that the structure of the two enzymes would in part differ. Wereport the ... while the Ne2 atom is hydrogenbonded to a n aspartic acid residue, albeit from a differentpart of the structure. The role of the t hreonine residue in the HXDT motif is less obvious, but the ... peptides [1].Many of these e nzymes h ave been shown to function in the degradation of intracellular proteins and in the catabolism of exogenously supplied p eptides [2]. O ne of these enzymes,peptidase...
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Tài liệu Báo cáo khoa học: Motion of the Ca2+ -pump captured ppt

Tài liệu Báo cáo khoa học: Motion of the Ca2+ -pump captured ppt

... as the ATP-unbound and Ca2+-boundE1Ca2+form. In the histogram (Fig. 2A) of the dis-tribution of the height of the projection of the embed-ded molecule above the flat membrane surface, the average ... Histograms of the height differences between the top of SERCA and the surface of the membrane. Statistical section analy-ses of SERCA were performed with the data obtained in the pres-ence of (A) ... presence of both ATP andCa2+were excluded from the following analyses.Visual characteristics of the Albers–Post scheme The number of peaks (i.e. the number of up–downconformational changes of...
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Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... lengthrestrictions of this minireview. The work of S.W. wassupported by the Deutsche Forschungsgemeinschaft(We2843 ⁄ 2 -1) and BGAG-Stiftung Walter Hesselbach. The work of S.B. is supported by grants from the ... to the formation of VirB2-VirB5 complexes followed by T-pilus assembly. The assem-bled T4SS then triggers the secretion of substrates from the bacterial cytoplasm directly into the cytoplasm of ... consist of 11 VirB proteins (encoded by virB1–virB11 genes) and the so-called coupling protein (the NTPase VirD4). The prototypic and best charac-terized T4SS is the VirB or T-DNA transfer system of Agrobacterium...
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Tài liệu Báo cáo khoa học: Upregulation of the a-secretase ADAM10 – risk or reason for hope? docx

Tài liệu Báo cáo khoa học: Upregulation of the a-secretase ADAM10 – risk or reason for hope? docx

... for a therapeutic approach in AD [13–15]. Protein structure and geneorganization of ADAM10 The enzyme ADAM10 belongs to the subgroup of metzincins within the zinc proteinases family. The typi-cal ... point of intervention within the biosyntheticpathway of ADAM10 is provided by directly interfer-ing with the expression of the gene for ADAM10: the promoter region of the gene for ADAM10 has beencharacterized ... treatment of AD, enhance the transport of ADAM10 to the cellsurface and the non-amylodogenic cleavage of APP[91,92].ADAM10 has also been shown to be regulated by the lipid composition of the plasma...
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Tài liệu Báo cáo khoa học: Myristoylation of the dual-specificity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf

Tài liệu Báo cáo khoa học: Myristoylation of the dual-specificity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf

... preferentially the phosphorylation of the p46 isoform of JNK,whereas sorbitol treatment induced phosphorylation of both the p46 and p55 isoforms. To confirm functionalactivation of the JNK signaling ... efforts were frustrated by the lack of specificity of SP600125, which has also been reported by others[42,43]. Resolution of the importance of JNK activa-tion will require further experimentation.In ... phosphorylation of the transcription factorc-JUN was attenuated. Upon expression of wild-type JSP1, a subpopula-tion of cells, with the highest levels of the phosphatase, was induced tofloat off the dish...
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Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc

Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc

... induce the synthesis of HSPs that act as molecular chaperonesthrough binding to the hydrophobic surfaces of unfolded proteins, thereby facilitating refolding of pep-tides and preventing protein ... development of the lens and maintenance of the olfactory epithelium [102]. The first evidence for a developmental function of HSF4 was provided by population genetic studies wheremutations of the Hsf4 ... organisms from bacteria to mammals [2]. By inducing transcription of the genes encoding heatshock proteins (HSPs) that function as molecularchaperones, the HSFs protect the cell from the delete-rious...
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Tài liệu Báo cáo khoa học: Switching of the homooligomeric ATP-binding cassette transport complex MDL1 from post-translational mitochondrial import to endoplasmic reticulum insertion pptx

Tài liệu Báo cáo khoa học: Switching of the homooligomeric ATP-binding cassette transport complex MDL1 from post-translational mitochondrial import to endoplasmic reticulum insertion pptx

... the intermembrane space of yeast mitochon-dria by a stop-transfer mechanism. Cell 69, 809–822.20 Stuart R (2002) Insertion of proteins into the innermembrane of mitochondria: the role of the ... innermembrane proteins [14]. The mitochondrial targeting signal (MTS) of pro-teins directed to the IMM is recognized by receptors of the translocase of the outer mitochondrial mem-brane (TOM complex). The ... and -11relative to the cleavage site. The leader sequence inter-acts with the TOM receptor, responsible for the trans-location of the preproteins to the translocase of the inner mitochondrial...
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Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc

Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc

... and to mea-sure the mobility of the components in the PGCs. The medaka embryo was peeled off the chorion, and the segment containing the part of PGCs was excised forobservation by microscopy and ... processes of replace-ment of molecules in granules from other parts of the same granules or from the cytosol. We performed ableaching experiment in a whole part of a single com-partment of the granule, ... on the same setup of the laser scanning microscope as used for FCS analysis. The detection gain was adjusted to the fluorescence of the GFP fusion proteins almost at the saturation level of the detector,...
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