Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural speciﬁcity variants doc

... FEBS Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural speciﬁcity variants Gabor L. Igloi and Elfriede SchiefermayrInstitute of Biology, University of ... betweenarginine and canavanine for aminoacylation of the planttRNA transcript was observed when using [14C]-canava-nine. At 0.4 mm canavanine, less than 10% of the tRNAwas aminoacylated compared ... that of HNONH OHL-Arginine H2NNNH2NH2NH2NH2OOHOL-Canavanine Fig. 1. Structures of L-arginine and its guanidinooxy analogue,L-canavanine. Arginyl-tRNA synthetase amino acid discrimination...

Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt

... acknowledged for theirassistance with data collection. We thank AnnetteKure Andreassen, Marianne Mortensen and AugustaPalsdottir for outstanding technical assistance.References1 Huang W, Jia J, Edwards ... FEBSDecarboxylation of malonyl-ACP by wild-typeand mutant KAS I proteinsThe ability of the wild-type and mutant KAS I proteinsto form acetyl-ACP from malonyl-ACP (Scheme 1) wasmeasured by visualizing ... mutants had been obtainedwas achieved by complete sequencing of the initial trans-formants in XL1-Blue cells.Expression, puriﬁcation and activity assayWild-type and mutant His-tagged KAS...

Tài liệu Báo cáo khoa học: Fatty acid desaturases from the microalga Thalassiosira pseudonana pptx

... completion of the genome sequence of the EPAand DHA producing diatom T. pseudonana [17]. Levels of EPA and DHA in this organism are in the range of 17 and 5%, respectively, when in the exponential phase of ... hasrecently been isolated from a number of marine organ-isms [12–14]. The ﬁnal elongation step [of C20 polyun-saturated fatty acids (PUFAs) to C22] catalysed by aD5-elongase was the last ... [18].Sphingoid base analysisSphingolipid analysis of yeast cells was carried out essen-tially as described previously [21]. LCBs were liberatedfrom yeast cells by alkaline hydrolysis and extracted...

Tài liệu Báo cáo khoa học: Multifunctional host defense peptides: Antimicrobial peptides, the small yet big players in innate and adaptive immunity docx

... secretion of hBD3 andother antimicrobial peptides by human keratinocytes,after the disappearance of neutrophils and before there-establishment of the physical barrier, in sterilewounds as well as ... [25], and gastric epithelial cells [26]. hBD1 andhBD2 have predominant antibacterial activity againstGram-negative bacteria and some fungi, whereashBD3 has a broader spectrum and kills many ... release of histamine and prostaglandin D2in a G-protein–phospholipase C-dependent manner [62] [71]; HPN1–3bind C1q and activate the classic complement pathway[72], increase the production of...

Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf

... cell invasion andmetastasis, although their role is dual [41–43]. BothMMPs and TIMPs, as well as the in vivo substrates of a given MMP, can prevent or facilitate the invasionand metastasis process, ... syn-thesis and activation of MMP-2, as well as synthesis of MT1-MMP andTIMP-1, by using an in vitro model with 3D ﬁbrillar and 2D monomericcollagen. In order to reveal whether the metastasis-associated ... indecreased proMMP-2 activation and reducedMT1-MMP expressionA decrease in the total amount of MMP-2 (72, 64 and62 kDa bands), as well as in the active MMP-2 forms(64 and 62 kDa bands), was observed...

Tài liệu Báo cáo khoa học: Is ATP binding responsible for initiating drug translocation by the multidrug transporter ABCG2? docx

... membrane protein con-sisting of six transmembrane helices and an amino terminal nucleotide binding domain (NBD) [9–11]. It isdescribed as being a ‘half-transporter’ as the canonicalABC transporter ... (3–300 lM) as described in Materials and methods.Labelled protein was visualized and quantiﬁed by autoradiography of SDS-PAGE analysis. The amount of bound protein was plotted as a function of nucleotide ... ﬁltration assay, and theamount of bound radioligand was determined by liquid scintillationcounting. Values refer to the mean ± SEM of at least three inde-pendent membrane preparations, and the...

Tài liệu Báo cáo khoa học: 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5¢-phosphate synthases of fungi and archaea docx

... measurements. 3-(trimethylsilyl)propanesulfonateserved as an external standard for1H- and13C-NMRmeasurements.Assay of 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5¢-phosphate synthase ... addition of guanylate kinase and pyruvate kinase served to recycleGMP, a by- product of GTP cyclohydrolase II, intoGTP). 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5¢-phosphate synthase was added ... cyclohydrolase II, 2 units of guanylate kinase and 2 units of pyruvate kinase in a total volume of 0.5 mL. 2,5-diamino-6-ribityla-mino-4(3H)-pyrimidinone 5¢-phosphate synthase from Methano-caldococcus...

Tài liệu Báo cáo khoa học:Insulin-like growth factor 1 signaling regulates cytosolic sialidase Neu2 expression during myoblast differentiation and hypertrophy doc

... reaction was terminated by addi-tion of 0.8 mL of 0.25 m glycine buffer (pH 10.4), and theamount of 4-methylumbelliferone released was determinedﬂuorometrically with an excitation wavelength of 365 ... Riboni M, Manzoni M,Venerando B, Croci G, Preti A, Ballabio A, TettamantiG et al. (2000) Identiﬁcation and expression of NEU3,a novel human sialidase associated to the plasma mem-brane. Biochem ... cytosolicsialidase Neu2 expression during myoblast differentiationand hypertrophyAlessandro Fanzani, Francesca Colombo, Roberta Giuliani, Augusto Preti and Sergio MarchesiniDepartment of Biomedical...

Tài liệu Báo cáo khoa học: The most C-terminal tri-glycine segment within the polyglycine stretch of the pea Toc75 transit peptide plays a critical role for targeting the protein to the chloroplast outer envelope membrane ppt

... Exchanging this portion with a repeat of leucine or glutamic acid also caused missorting of Toc75 to the stroma. By contrast, its replacement with repeats of asparagine, aspartic acid, serine,and ... stroma and to themembrane (Fig. 4A, lanes 21–24; Fig. 4B,C) in a waysimilar to GGA mutant (Fig. 4A, lanes 9–12;Fig. 4B,C). Import of GGE mutant was less efﬁcientthan that of other proteins, as ... second domain of the pea Toc75 tran-sit peptide consists of 96 amino acids [11]. It is neces-sary to target the protein to the outer envelopemembrane [13], and is cleaved off by a plastidictype...

Tài liệu Báo cáo khoa học: 1,5-Diamino-2-pentyne is both a substrate and inactivator of plant copper amine oxidases ppt

... DABY and PSAO (r ¼ 17) [7]. From thispoint of view, plant CAOs are more resistant to theinactivation by DAPY than by DABY. Binding of DAPYat the active site of GPAO is dependent on both pH andionic ... values)in the inactivated enzyme was conﬁrmed. As in the case of DABY, the cofactor topaquinone is not modiﬁed by thereaction, as demonstrated by an unchanged quinone redoxstaining.Absorption ... mixture was ana-lyzedbyESI-IT-MSasdescribedinMaterialsand methods. (Lower) A combined solution of DAPYandACA(each5mM)in0.1Mammonium bicarbonate, pH 7.8, was mixedwith an excess of GPAO and...

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