Tài liệu Báo cáo khoa học: Mitogen-activated protein kinase phosphatase-1 modulated JNK activation is critical for apoptosis induced by inhibitor of epidermal growth factor receptor-tyrosine kinase pdf

... in epidermal growth factor- mediated protection against apoptosis induced by a DNA-damaging agent. FEBS J 273, 3743–3755.K. Takeuchi et al. JNK activation is critical for AG1478 -induced apoptosis FEBS ... apoptosis FEBS Journal 276 (2009) 1255–1265 ª 2009 The Authors Journal compilation ª 2009 FEBS 1265 Mitogen-activated protein kinase phosphatase-1 modulated JNK activation is critical for apoptosis induced ... apoptosis induced by inhibitor of epidermal growth factor receptor-tyrosine kinase Kenji Takeuchi1, Tomohiro Shin-ya1, Kazuto Nishio2and Fumiaki Ito11 Department of Biochemistry, Faculty of...

Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx

... typical growth factor signalling pathway, such as receptor protein tyrosine kinase/ growth factor receptor binding protein 2-son of sevenless protein/ Ras/Raf/mitogen activated protein kinase kinase/mitogen ... activated protein kinase pathway [13].Another function of the PKB signalling pathway is thepromotion of cell survival or inhibition of cell apoptosis [3,14]. PKB is a general mediator of survival ... magnitude of expression was indicatedas the ratio of the intensity of a-1,3 FucT-VII band to theintensity of GAPDH band.Statistical analysisStatistical analysis was performed with SPSS software...

Tài liệu Báo cáo khoa học: Prion protein library of recombinant constructs for structural biology docx

... [26] for the numeration used in this article). This C-terminalfragment forms a globular domain both as part of thefull-length sequence and in the isolated form, and there-fore constructs of ... sequence of the mature cellular form of the prion protein from a variety of species and truncatedvariants thereof, and of human doppel. The protein accession number refers to the NCBI protein ... been performed only for the PrPs frommouse (Table 2), cattle, and humans. For hPrP, Table 1also lists a number of designed variants of hPrP(121–230), most of which have been inspired by natural...

Tài liệu Báo cáo khoa học: Multisite protein phosphorylation – from molecular mechanisms to kinetic models pdf

... 3187providing additional mechanisms for the generation of activation thresholds. This is particularly true for dis-tributive kinetics (with multiple binding ⁄ dissociationevents for kinase or phosphatase), ... Dalby KN (2001) The kineticmechanism of the dual phosphorylation of the ATF2transcription factor by p38 mitogen-activated protein (MAP) kinase a. Implications for signal ⁄ response pro-ﬁles of ... concentration of the kinase is much smaller than that of the target protein [28–30,82,83]. In this case, target proteins of variousphosphorylation states compete for the kinase (or,equally, for the...

Tài liệu Báo cáo khoa học: SREBPs: protein interaction and SREBPs Ryuichiro Sato doc

... ligase (E3) required for the ﬁnal reaction of SREBP ubiquitination is acomplex consisting of Skp1, Cul1, Rbx1, and one of afamily of F-box proteins, Fbw7 [17]. Glycogen syn-thase kinase- 3b phosphorylates ... to growth factor stimuli [21]. This implies that growth factor stimuli interfere with sumoylation, therebyenhancing SREBP transcriptional activities, and lipidsynthesis required for cell growth. ... (1999) Co-stimulation of promoter for lowdensity lipoprotein receptor gene by sterol regulatoryelement-binding protein and Sp1 is speciﬁcally disrupted by the Yin Yang 1 protein. J Biol Chem...

Tài liệu Báo cáo khoa học: Antioxidant protein 2 prevents methemoglobin formation in erythrocyte hemolysates doc

... mouse). As the same protein is supposed to have the same namein diﬀerent species, we will use the old name of antioxidant protein 2until this nomenclature issue is resolved by the human and mousenomenclature ... transferred to newmedium. Production of recombinant protein was induced by addition of isopropyl-b-D-thiogalactopyranoside. Cellswere harvested, disrupted and recombinant protein waspuriﬁed from the ... However, the protein detected in cells of mouse, human, rat and pig origin, as wellas the recombinant form of AOP2, migrates in PAGE as a protein of approximately 32 kDa. To date, the protein expression...

Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf

... sequence analysis of the cDNA for a human serineprotease responsible for activation of hepatocyte growth factor – structural similarity of the protease precursorto blood coagulation factor XII. ... representation of thestructure of the full-length HAI-2 (1) and a soluble form of HAI-2tagged at the C-terminals with myc-His (2). (C) Dose dependence of the inhibitory activity of soluble forms of HAI-1 ... regulated by endoge-nous protease inhibitors. However, protease inhibitors of most TTSPs havenot yet been identiﬁed. In this study, we investigated the inhibitory effect of hepatocyte growth factor...

Tài liệu Báo cáo khoa học: Helicobacter pylori acidic stress response factor HP1286 is a YceI homolog with new binding speciﬁcity pdf

... a two-fold axis, all of the inter-actions reported below are repeated twice; that is, if Ala25 of chain A is close to Asn77 of chain B, then Asn77 of chain A is close to Ala25 of chain B.Chain ... is shorter withrespect to that of the two homologous proteins whosestructure has been determined: its volume is 151 A˚3,whereas that of the T. thermophilus protein is 233 A˚3.This is ... subgroup of the lipocalinsuperfamily [15]. The prototype of lipocalins is reti-nol-binding protein (RBP), a protein of 182 aminoacids present in the plasma of higher animals, andresponsible for...

Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf

... similarities of tissue inhibitor of metalloproteinases-2 and tissue inhibitor of metalloproteinases-1. Biochem J 278, 179–187.16 DeClerck YA, Yean TD, Lu HS, Ting J & Langley KE(1991) Inhibition of ... softstructures of connective tissue. Collagens are the maincomponents of ECM, and type I collagen is the mostabundant form in bone and connective tissue [1].Controlled turnover of ECM is critical ... proliferation and apoptosis [11]. AlthoughS100A4 is strongly associated with the stimulation of invasion and metastasis, the actual mechanism for themetastasis-promoting function of S100A4 is not com-pletely...

Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc

... Murakami2and Kenji Aoki21Division of Science of Biological Resources, Graduate School of Science and Technology,2Department of Biofunctional Chemistry,Faculty of Agriculture, Kobe University, ... peak of the purified enzyme at 266 nm. The typical protein absorption p eak of 2 80 nm shifts to 265 nm if the protein contains a flavin-type cofac tor [28]. We failed toidentify the cofactor of ... ( B)the emission spe ctrum (excited at 450 n m) of the supernatant of theheat-treated enzyme (1.2 mg protein per mL). The cofactor derivedfrom the purified enzyme was relea sed by hea t treatme...

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