... [4,5]. The remaining 10 subunits of the mammalian enzyme, namely, IV, Vb, VIa, VIb, VIc, VIIa,VIIb and VIII are encoded by the nuclear genome,synthesized in the cytosol and imported into mitochondria[1–3]. ... Chaps and 150 mMNaCl and protease inhibitors [5 lgÆmL)1pepstatinA, 5 lgÆmL)1aprotinin, and 1 mMphenylmethanesulfonylfluoride]) and lysed by three cycles of freezing and thawing in liquid ... isolog H and L. The L isolog isubiquitously expressed in all the tissues whereas the H isologis detected in the heart and skeletal muscle tissues [3]. The Northern blot in Fig. 1E shows that in both...