Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: photosynthetic electron transfer from photosystem I to NADP+ doc

... AnFNR isoalloxazine N5 via S80[58,62]. In addition, in both enzymes, this residue iscritical for proper binding of the nicotinamide to theactive centre, CTC stabilization and efficient flavinreduction ... ferredoxin–NADP+reductase. Interactions that stabilize protein–flavin complexes and tailor the midpoint potentials in these proteins, aswell as many details of the binding and electron transfer to protein and ligand ... oxidation–reduction potentials of the Clostridium beijerinckii flavodoxin: effects of substitutions by aliphatic amino acids and evidence fora role of sulfur-flavin interactions. Biochemistry 37,9668–9678.37...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: electron transfer through the nitric oxide synthase ﬂavoprotein domain pdf

... 273,22267–22271.48 Sato Y, Sagami I & Shimizu T (2004) Identiﬁcation of caveolin-1-interacting sites in neuronal nitric-oxide syn-thase. Molecular mechanism for inhibition of NO for-mation. J Biol Chem ... enzymeswill certainly improve our understanding of this essen-tial FMN subdomain interaction.Relationship of KeqA to equilibrium B and to NOS heme reductionAt the limit, KeqA can impact ... the rate of FMNH2formation without causingcoincident changes in conformational equilibrium A and in the kon and koffparameters is still unavailable.Indeed, CaM shifts equilibrium A in NOS...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: probes of hydrogen tunnelling pptx

... and kinetic isotope effects using PEG-modiﬁedvariants and glycoforms of glucose oxidase: therelationship of modiﬁcation of the protein envelope to C–H activation and tunneling. Biochemistry 41,8747–8758.72 ... tunnellingSam Hay, Christopher R. Pudney and Nigel S. ScruttonManchester Interdisciplinary Biocentre and Faculty of Life Science, University of Manchester, UKIntroductionThere is now fairly widespread ... !ð12Þwhere r, in units of viscosity, is the contribution of the protein friction to the total friction of the system.The activation entropy and enthalpy can be deter-mined independently from the...

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... a-helices.b4 in the core domain extends into a b-ribbon with b5,stabilizing loop II. The hood domain is mainly builtup by residues from the C-terminus and consists of atwo-stranded anti-parallel ... Department of Medical Biochemistry and Biophysics,Karolinska Institutet, S-17177 Stockholm,Sweden; Department of Anatomy,Physiology and Biochemistry, SwedishUniversity of Agricultural Sciences, ... the purine base and Asp137 was observed in HPRTin complex with ImmGP, indicating a role in transitionstate stabilization during catalysis [17].A series of mutations were made of the invariantAsp...

Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt

... forcompetitive and noncompetitive inhibitors. For competitiveinhibition, the equation is v ¼ VmaxÆ[S] ⁄ (Km(1 + [I] ⁄K i ) + [S]), and for noncompetitive inhibition the equationis v ¼ VmaxÆ[S] ... the kinetic analysis showed posi-tive cooperativity, with an n value of 1.5 ± 0.1. The end-product UTP wasa competitive inhibitor against UMP and a noncompetitive inhibitortowards ATP. Unlike ... carried out in o [29] and coot [30].In chain A, residues 1 and 169–176 are missing; inchain B, residues 167–172 are missing; in chain C, residues1, 2 and 169–174are missing; in chain D, residues...

Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx

... extends from the penton base atthe vertex positions. Additionally, cementing proteinssuch as IIIa, VI, VIII and IX help stabilize the capsid,reinforcing the penton–hexon and hexon–hexon inter-actions. ... exhib-ited impaired endocytosis. Interestingly, in this experi-ment, the fibers were not shed from the virus [19].These data raise the possibility of coupling of fiberrelease to binding of ... bound to the penton base, [LigB] is the concentration of the bound ligand (minifiber), and [Ligtot] is the total con-centration of the ligand. At any total concentration of lig- and, the anisotropy...

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... amplifiedusing gene-specific primers with BamHI and HindIII recogni-tion sites. The resulting PCR fragments were purified and double-digested using BamHI and HindIII, and cloned intopET28a(+) to generate ... genetic and biochemical studies are stillrequired to elucidate the in vivo function of HYDJs,itisspeculative that HYDJsmight also be involved in thedegradation pathway of pyrimidines in ... Production of d-amino acids from d,l-5-substituted hydantoins by an Agrobacterium tumefaciensstrain and isolation of a mutant with inducer-indepen-dent expression of hydantoin-hydrolysing activity.Biotechnol...

Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

... have important roles in energy metabolism and adenine nucleotide equilibrium. Inhibition of leishmaniagrowth with Ap5A, an inhibitor of leishmanial AK2 and itspartial reversal by ADP indicate ... the inclusionbodies and refolded in the presence of dithiothreitol,MgSO4 and KCl showed significant enzyme activity. AKactivity was linear in terms of both incubation time (up to 90 min) and ... mean of two different experiments.Fig. 6. Dixon plots of the inhibition of recombinant AK by the specificinhibitor Ap5A. Six micrograms recombinant L. donovani AK wereincubated in presence of...

Tài liệu Báo cáo khoa học: Structural and functional studies on a mesophilic stationary phase survival protein (Sur E) from Salmonella typhimurium ppt

... Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India2 Department of Biochemistry, Indian Institute of Science, Bangalore, IndiaDuring stress and the stationary phase of growth,bacterial ... crystallization of the proteinThe surE gene from S. typhimurium was cloned in anisopropyl thio-b-d-galactoside (IPTG)-inducible vector,overexpressed in E. coli and puriﬁed to homogeneityusing Ni-nitrilotriacetic ... Bilodeau D & Beliveau R (1999) Inhibition of GTP-gammaS-dependent L-isoaspartyl protein methylationby tyrosine kinase inhibitors in kidney. Cell Signal 11,45–52.6 Visick JE, Ichikawa JK &...

Tài liệu Báo cáo khoa học: Structural and functional speciﬁcities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx

... the dimer is formed(Fig. 3B). Six of the conserved cysteines are engaged inthree intrachain disulfide bonds (Cys I- VI, III-VII,V-VIII) stabilizing the cystine knot structure, whiletwo cysteines ... first immuno-globulin-like domains (reviewed in [64]). The residuesin PDGF-A and -B responsible for receptor bindingreside in loop 2, in addition to RKK161 in PDGF-AA and R27 and I3 0 in PDGF-BB. ... metalloproteinase inhibitor, TIMP-1, in dermalfibroblasts in vitro [78]. The PDGF-C induction of fibro-sis starts with activation and intense proliferation of thehepatic stellate cells, producing robust...

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