... reported concerning the functions and relationship of the dual domains. Here we describe the physical properties that relate to the catalysis of anewly isolated, dual-domain BPP (PhyH) from Bacillussp. ... pH(6.0–8.0) and at low temperatures (0–35 °C). We focuson the function and relationship of the two singledomains. Additionally, the possibility that fusion of the PhyH N-terminal domain to other single-domain ... S1). There are two pairs of cysteine residues, Cys142 and Cys193 in PhyH-DII and Cys445 and Cys495 in PhyH-DI. These residuesare at the same positions as Cys157, Cys206, Cys450 and Cys498 of PhyS...