Tài liệu Báo cáo khoa học: Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efficacy pdf
Tài liệu Báo cáo khoa học: Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efficacy pdf
... siRNAs versus siRNA 396was significant at P < 0.05.ABFig. 5. Effect of terminal and internal mismatches on siRNA TRBP and siRNA Dicer complexes. (A) EMSA of siRNA TRBP and siRNA Dicer complexes ... function of TRBP in RNAi.Here, we have characterized the interactions of siRNAs that contain terminal mismatches with TRBP and Dicer, and determined the impact of these interac-tions on their silencing ... thedose-responsive concentration range (Fig. S1). Effect of TRBP or Dicer knockdown on the silencing efficacy of mismatched siRNAsWe hypothesized that the reduction in the function of the mismatched siRNAs...
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Tài liệu Báo cáo khoa học: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr docx
... mm enhanced the binding of InsP6to PhyAsr (Table 1). Given the absence of structural changes as a function of ionic strength and ligand binding, the effect of ionic strength on catalyticactivity ... absence of ligand, the WPD loop of PTP1B and RPTPa adoptsan open (inactive) conformation, and upon ligand bind-ing, it adopts a closed (active) conformation (Fig. 3A).In oxidized PTP1B [12,13] and ... whether theP-loop moves upon oxidation of the catalytic cysteine isthe ability of the conserved active site arginine to move Effect of ionic strength and oxidation on PhyAsr R. J. Gruninger...
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Tài liệu Báo cáo khoa học: Effect of deletion of the DNase I hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus docx
... role of this region in the transcription of theIg-b gene. Deletion of region III decreased the expres-sion of Ig-b mRNA to 40% of the expression in prede-letion cells (Fig. 4A). Region III ... hybridization. Position of the probes, the size and position of restriction fragments hybridizable with the probe, and exon ⁄ introns areshown as in Fig. 2A. Positions of the DT40 specific-DHSs and ... estab-lishment and maintenance of acetylation in the GHlocus. Because deletion of this region has no effect on the formation of cell type-specific DHSs, the regionrequired for H3 and H4 acetylation does...
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Tài liệu Báo cáo khoa học: Effect of gadolinium on the ryanodine receptor/ sarcoplasmic reticulum calcium release channel of skeletal muscle docx
... cation binding site has been des-cribed so far. As the regulatory effect of the transcalcium concentration was voltage-dependent and changed as the function of the cis EGTA concentra-tion, ... several cytoplasmic ligands. It is activa-ted by millimolar concentrations of ATP and micromo-lar Ca2+, and inhibited by higher concentrations(> 100 lm)ofCa2+ and by millimolar Mg2+. ... pore [10]. Results about the effect of theluminal (trans) calcium on the activity of the RyR1 arecontroversial depending on experimental conditions.Inhibition of the channel was described...
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Tài liệu Báo cáo khoa học: Effect of sequence polymorphism and drug resistance on two HIV-1 Gag processing sites pdf
... analyzer. Stock solutions and dilutions weremade in distilled water and the peptide concentrations weredetermined by amino-acid analysis.Construction and purification of HIV-1 PR mutantsHIV-1 ... thesubstrate binding site of the PR, and these mutationshave considerable impact on PR activity and specificity.Other resistant mutations alter residues outside of thesubstrate binding site. ... determinedunder pseudo first order conditions using 0.02–0.15 mMconcentration range, in which the activity was a linearfunction of the substrate concentration. The standard errorfor kinetic values...
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Tài liệu Báo cáo khoa học: "Effect of Cross-Language IR in Bilingual Lexicon Acquisition from Comparable Corpora" pot
... termcorrespondences are estimated, in terms of both computational complexity and the performance of precise estimation of bilingual term correspondences.1 IntroductionTranslation knowledge acquisition ... situ-ation. One limitation of the corpus-based trans-lation knowledge acquisition approach is that thetechniques of translation knowledge acquisitionheavily rely on availability of parallel/comparativecorpora. ... expressions, and collocational expressionsfrom the collection of bilingual news articles on WWW news sites (Utsuro and others, 2002).Figure 1 illustrates the overview of our frame-work of translation...
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Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 fi 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf
... afunction of dextran concentration, the activity of GSGB was highly dependent on dextran (filled circlesin Fig. 2B); however, that of GS was nearly zero, inde-pendently of dextran concentration ... reciprocal plot of nigerotriose accep-tor reaction of GS.Fig. S4. Separation of nigerooligosaccharides by acti-vated carbon chromatography.Doc. S1. Simulation of the glucan synthesis reaction.This ... decanting and centrifugationat 600 g for 4 min, and was subsequently washed withwater by suction filtration. The structure of the productwas confirmed by13C-NMR spectroscopy.Preparation of nigerooligosaccharidesThe...
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Tài liệu Báo cáo khoa học: Degradation of tropoelastin by matrix metalloproteinases – cleavage site specificities and release of matrikines pptx
... The formation of pyroglutamic acid moieties from Glu and Gln were considered as variable modifications.Moreover, automated de novo sequencing of the nano-ESI–qTOF and MALDI-TOF ⁄ TOF MS data ... com-ponents of the extracellular matrix (ECM). MMPsplay a central role in connective tissue remodeling pro-cesses and regulation of cell matrix compositionthrough their effects on cell migration, ... analysisAnalysis of the digests was conducted by of ine nano-HPLC ⁄ MALDI-TOF ⁄ TOF MS, using a nanoHPLC sys-tem (Ultimate 3000; Dionex, Idstein, Germany) and anUltraflex III MALDI-TOF ⁄ TOF mass spectrometerequipped...
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Tài liệu Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide pptx
... demonstrate strong modulation of expression after stimulation with LPS and LP. LP and LPS mediate immunomodulating functions, at leastin part, through regulation of RGS1 and RGS2expression.ResultsRGS1 ... RGS1 and RGS2 mRNA and modu-late expression after stimulation with LP and LPS.Expression patterns of RGS1 and RGS2 afteractivation of TLR3 and TLR9To investigate the expression levels of RGS1 ... proving the responsibility of the TRIFactivation for the upregulation of both RGS mRNA.Stimulation of TLR3 and activation of the TRIF path-way leads to interferon-b production [35]. Takaokaet...
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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
... this, UV-irradiation of the cells (2.4 JÆm)2), althoughinducing a strong activation of JNK1/2 and only a veryweak phosphorylation of p38, had no effects on C TGFexpression, which ruled out ... expressionoccurs via increased transcription and/ or by stabilization of the CTGF transcripts and t he role of RhoA GTPase and p38 signaling in such a regulation, nuclear run -on assays and message ... without effects (data not shown).Higher concentrations were not used to avoid potentialnonspecific and/ or toxic effects. In contrast, anisomycininduced CTGF/CCN2 expression over a wide range of concentrations...
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