...
where t
i
and w
i
represent the occurrence (binary
value: 0 or 1) of words T and W in i-th document
respectively, and n is the total number of
documents in the collected documents. This ... words; it is generally
constructed as a middle size of a sentence and a
document. We then extract core contexts that
include at least one of the title words and the
keywords...
... structure, and their molecular environment.
In complex systems such as ce lls, the main absorptions
arise from N–H, C ¼O, C–H and P¼O bonds from the
proteins, lipids, and nucleic acids present in the ... samples o f the cell line A and a test set c omposed of 30
spectra of cell line A and cell line B. The trainin g set was the
only one used for model calculations (PCA, g...
... 2927–2937 ª 2011 The Authors Journal compilation ª 2011 FEBS
In the work presented here, P216A and P233A
mutant tau proteins were correctly synthesized and
expressed in CHO cells, and both of these proteins
were ... 2927–2937 ª 2011 The Authors Journal compilation ª 2011 FEBS 2933
by alanines, a QuikChange XL site-directed mutagenesis
kit (Stratagene, Amsterdam, the Netherlands)...
... required before the NLS
unfolds into an extended and exposed sequence that is
recognized by the import receptor.
Materials and methods
Protein expression and purification
The generation of the mouse ... as
the major and minor binding sites [1]. These sites are
located in the concave face of the protein near regions
of invariant Trp and Asn arrays. The major binding
si...
... rat [27] and human [26] HSS
genes, and some orthologous genes that have been
identified in dsDNA viruses [28] have together been
defined as the ERV ⁄ HSS gene family [29]. Members of
this family ... lm) was then
added to the mitochondria, and the CCCP-treated mito-
chondria were incubated at 4 °C for 4 h. The mitochondrial
suspension was then centrifuged at 12 000 g for 5 mi...
... cotyledons and mature leaves, whereas, in
the flowers, the anthers, connectives and filaments and
the base and tip of the stigmata were stained. Similar
to BPM2, BPM3 and BPM5 promoter:GUS lines, the
root ... using the BamHI and
XbaI restriction sites, the promoters were fused to the GUS
gene in the binary vector pCB308 [41]. The primers used in
this and othe...
... Fabry disease, the primary pathol-
ogy is in the kidney [1], the major site of Gb
3
synthesis
in man [11], and in the heart, possibly due to the
association of Gb
3
synthesis with the microvasculature.
GSL ... Gb
3
accumulated in
Kupffer cells, endothelial cell subsets within the central and portal vein and
within the portal triad. Hepatic venule endothelial and Kupffe...
... assay, the enzyme
reaction product identified by GC-MS analysis, and the
determination of released ammonia. The coupled enzyme
assay revealed the mechanism of the deamination reaction
and the subsequent ... 4.2-kb
fragment does not contain the gene encoding the deaminase
reported here. In the cloned 5.2-kb fragment from P. pseudo-
alcaligenes JS45, there are no genes in...
... polyspermy, and protecting the embryo
until implantation [1,2]. The zona is composed of three
glycoproteins, called ZPA, ZPB and ZPC in the order of the
size of their cDNAs [3], and their carbohydrate ... difficult.
The N-linked chains of the bovine zona protein mixture
include neutral (23%) and acidic (77%) chains [14]. We
have determined the structures of the neutral...
... is
the design of the multilevel structures,
while the grammar formalism is the tool (notation)
for expressing these multilevel structures, their
related sentences, and the nature of the correspon- ... have omitted the other subcorrespon-
dence between substrings and subtrees, for example
between the whole TREE and the whole STRING (tri-
vial), between the subtr...