... The intracellular concentration of heme
is maintained by the rate of its synthesis and degrada-
tion [1]. Many enzymes and their regulators are
responsible for heme synthesis [1,2]. On the other
hand, ... in the accumulation of heme in the hemin-
treated HepG2 cells. Taken together with the siHO-2-
mediated induction of HO-1 expression, these results
suggest that HO-2 rather...
... melanoma cell line, but in contrast to what was
observed in RAW 264.7 cells, the mutant was able to
efficiently kill these cells. These data are consistent
with the notion that induction of pyroptosis ... suggests that different types of cells
are killed by LF as a result of the cleavage of distinct
substrates.
To summarize, we have isolated an LF mutant that
is impaired in its abi...
... In the case of the T domain, the MG
state corresponds to the functional state, which initi-
ates the translocation of the catalytic domain. Here,
the data allowed identification of the core of the ... structure, TH5 is partly accessible at the
surface of the T domain. We propose that its high protection is caused by
the formation of dimers. Within the molten...
... Ð
k
f
a2
k
r
a2
S
a2
Á S À!
k
cat
a2
S
a2
þ S
a1
ð1Þ
The autophosphorylation rate (v
3
) is the sum of the
rates catalyzed by each form. Applying quasi steady-
state (QSS) approximation for the intermediate com-
plexes, ... and the activatory residue is dephosphorylated; S is the
partially active form, where both the inhibitory and activatory resi-
dues are dephosphoryla...
... that there is
always a K
eq
position for maximum electron flux
through the enzyme. On either side of this optimum,
the electron flux drops off because either the formation
rate (k
2
) or dissociation ... kinetic model for NOS catalysis. Ferric enzyme
reduction (k
r
) is rate limiting for the biosynthetic reactions (central
linear portion). kcat1 and kcat2 are the conversion...
... induced by ischemia during AMI or
PCI remain to be elucidated. Delineation of the
molecular basis for our observations is essential to
evaluate the elevated DNase I activity in the sera of
patients ... indi-
cated by overbars. The underlines repre-
sent the locations of the two
oligonucleotide probes used for further ana-
lysis. The position and identity of mutations
at...
... to study the tissue-specific use of
each promoter. We show the translation initiation site
of isoform 2 and different intracellular distribution of
isoforms 1 and 2, and discuss the regulatory mechan-
ism ... initi-
ation sites.
Determining the translation initiation site of
isoform 2 KLK11
Messenger RNA for isoform 2 contains two initiation
codons near the 5¢ end. To examine wheth...
... droplet
accumulation, respectively. Phosphatidylserine is a
lipid that is enriched in the inner face of the plasma
membrane and that is translocated into the outer face
under certain cellular states. ... with ferric citrate. Rather, these
oxidants induced cell death of HepG2 cells (data not
shown).
Upregulated CD1d expression and alterations
in lipid parameters
Considering that...
... stimulated for 5 min if not
indicated otherwise. All experiments were carried out in
duplicate at least three times, if not otherwise indicated.
Western Blotting
Equal amounts of cell lysate proteins ... induces the activation
and phosphorylation of Ras-GRF1. Furthermore,
Ras-GRF1 is also heavily phosphorylated upon agon-
ist activation of GPCRs, but the exact role of these
phosph...