Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc

Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

... MAPKERK1153755PPPp53Fig. 1. Regulation of DNA fragmentation by phosphorylation of the MAPK family and mitochondrial proteins. Phosphorylated ERK prevents the activation of caspases and the Bcl-2 family, whereas these ... mitochondria during apoptosis. They induce the permeabilization of the mitochondrial membranewith the release of cytochrome c and the formation of the apoptosome [38]. The major pro-apoptotic proteinBax ... BNIP3 and BimL). Bcl-2 family proteins mostly mediate the activity of otherproteins in the same family [74]. Antiapoptotic Bcl-2 family members bind to pro-apoptotic family mem-bers, interrupting...

Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc

... (2001) Roles of the heat shock transcription factors in regulation of the heat shock response and beyond. FASEB J 15,1118–1131.4 Fujimoto M & Nakai A (2010) The heat shock factor family and ... 2010 The Authors Journal compilation ª 2010 FEBS 4139MINIREVIEW Regulation of the members of the mammalian heat shock factor family Johanna K. Bjo¨rk1,2and Lea Sistonen1,21 Department of ... stimulus-speciﬁc gene regulation, strict control of the transcription factors themselves is crucial. Heat shock factors are a family of transcription factors best known as master regulators of inducedgene...

Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx

... 133–138 at the exposed part of the cathepsin B molecule near the occluding loop(Fig. 2C). The location of the epitope indicates that the binding of 2A2 mAb might change the conforma-tion of the loop, ... of 161.8, 28.2 and 13.2 kDa, respectively. The formation of the cathepsin B ⁄ cystatin C complexwas seen as a shift of the elution peak of cathepsin B(Fig. 6A). The addition of 2A2 mAb to the ... which the N-terminus of the inhibitor ﬁrst binds to the enzyme, displacing the occluding loop, followed by the binding of another two loops of the inhibitor [24].Besides protein inhibitors, the...

Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

... in the main chain of the subunit, or in the interaction of subunits within the trimer that linked the conformation of residues 120–125 to the position of the active site lip and closure of the ... movement of the lip formed bya helix 2 and b strand 5 [18]. Apparently, movement of the active site lip prevented binding of the C-terminalresidues over the active site, or the absence of the C-terminal ... mechanism of regulation that generates the apparently attractive feature of anincrease in both S0.5and the sigmoidity of the satura-tion curve for dCTP in response to the binding of dTTP to the...

Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx

... termination of the pr otein s ynthesis.These data a dd to the growing body of informationsupporting a preponderant role of p38 in the regulation of gene expression at the level of mRNA stability. The ... further establish the speciﬁcity of action of RhoGTPases on CTGF/CCN2 expression, we examined the ability of the constitutively active form s of Rho GTPases t oenhance the expression of the ... nd modulate the activity of transcription factorseither via direct physical interactions or by sequesteringcofactors required for their activation [29]. The ﬁnalunderstanding of the underlying...

Tài liệu Báo cáo khoa học: Regulation of the actin–myosin interaction by titin doc

... remained in the pellet independently of the other components and the ionic strength of the mixture.In the presence o f A TP, the amount of bou nd H MM wasvery similar (average value of 52.5 ± ... solution. On the other hand, the acceleration of the sliding velocity (and the lower number of stops) of thin ﬁlaments could be related toa diminution o f the load in the actin–myosin interface. The ... in the absence of Ca2+, inde-pendent of the addition of T800. This result, togetherwith the fact that T800 increased the average andmaximum velocity v alues of moving ﬁlaments, both inthe...

Tài liệu Báo cáo khoa học: Afﬁnity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx

... PCSK9-S127R, only the upperand lower, respectively, halves of the ranges are shown, to avoidoverlap of the error bars. The results are expressed as percentage of control (c.p.m. in the absence of unlabeled ... reduced from 19 to 16 minand the half-time of the slow phase was reduced from270 to 154 min. In the presence of unlabeled PCSK9-D374Y, the half-time of the rapid phase of [125I]TC-PCSK9-D374Y ... increased the propor-tion of the slowly dissociating component, at the expense of the component with rapid dissociation. Thisresult cannot be simply explained by the presence of two populations of...

Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc

... (grey). Doc. S1a. Model structure of C24. Doc. S1b. sbml format of model structure of C24. Doc. S2a. Model structure of Rsch. Doc. S2b. sbml format of model structure of Rsch. Doc. S3. Documentation ... & Stitt M (1993) Reg-ulation of the expression of rbcs and other photosyn-thetic genes by carbohydrates – a mechanism for the sink regulation of photosynthesis. Plant J 3, 817–828.29 Moore ... (unit:C6h)1Æg)1FW):rCO2!Suc¼ rNetPhotosynthesisÀrCO2!StarchRatesof net photosynthesis (rNetPhotosynthesis) were calcu-lated as the average rate of carbon uptake during the ﬁrsthalf of the light phase (n =...

Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

... [46]. The similarity in the Kmvalues is attributable to the uracil moiety bindingin the same fashion [38], which could trigger the initialbinding of both substrates. In UpG, the binding of the guanine ... [42]. The structure of N-acylsulfonamide 7 was reﬁned with full occupancy,except for the alternative conformations observed for the N-acylsulfonamidyl group and the addition of O2¢. The value of ... formed by the uridine ribose. Of the two hydrogen bonds exhibited by these twoatoms, one is a direct interaction with the enzyme and the other is mediated by a water molecule. In the com-plex...

Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc

... disappearance of the heavier inactive peak. Furthermore, FPLC analysis of the recombinant wild-type cN-II showed the presence of the tetrameric active form irrespective of the pres-ence of effectors. ... determine the value of K50for thesecompounds. Under these experimental conditions, the accu-mulation of radiolabelled inosine (nucleotidase activity) rep-resents the sum of the phosphatase and the phosphotransferase ... site The soluble 5¢-nucleotidase family shares four con-served motifs with the HAD superfamily that areinvolved in the reaction mechanism [5]. Resolution of the crystal structure of some family...

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