Tài liệu Báo cáo khoa học: The multicopper oxidase from the archaeon Pyrobaculum aerophilum shows nitrous oxide reductase activity docx

... Journal compilation ª 2010 FEBS 3189 The multicopper oxidase from the archaeon Pyrobaculum aerophilum shows nitrous oxide reductase activity Andre´T. Fernandes1, Joa˜o M. Damas1, Smilja ... transfer; DSC, differential scanning calorimetry; MCO, multicopper oxidase; McoP, multicopper oxidase from Pyrobaculum aerophilum ;N2OR, nitrous oxide reductase; RR, resonance Raman;SGZ, syringaldazine; ... to nitrite, nitrite to nitric oxide, nitric oxide to nitrous oxide, and ﬁnally nitrous oxide to dini-trogen [20,21]. The nitrate and nitric oxide reductasesof P. aerophilum have been isolated...

Tài liệu Báo cáo khoa học: An alternative transcript from the death-associated protein kinase 1 locus encoding a small protein selectively mediates membrane blebbing pdf

... full-length DAPK-1, although the effect was weaker(Fig. 4B). Given the biological activity of s-DAPK-1 in the membrane-blebbing assay, we evaluated the activity of the mutant with the tail deletion (Flag-TD; ... supporting the existence of a protease thatcleaves s-DAPK-1 protein in vivo. The reason why the cleavage band was not observed in this assay may be the rapid degradation of the puriﬁed protein from the cell ... stops at the 126th base pair of intron 20–21 of the DAPK-1 gene, and the 3¢-UTR extends through the middle ofintron 20–21. (B) Comparison of the protein sequences of DAPK-1 and s-DAPK-1. The ﬁrst...

Tài liệu Báo cáo khoa học: Fatty acid desaturases from the microalga Thalassiosira pseudonana pptx

... and other proteins involved at the biochemical level. Such a situation now exists for dis-covery of genes involved in PUFA biosynthesis follow-ing the completion of the genome sequence of the ... in the TpdesO gene. The amino acid sequenceof PtDEL5 and TpDESO exhibited 63% identity, sug-gesting that TpDESO could catalyse the D5-desatura-tion step in the PUFA biosynthetic pathway. The TpDESK ... notshown). The precise regiospeciﬁcity of the activity encoded by TpdesB was further investigated by comi-gration with authentic standards for desaturateddihydroxy-LCBS. This indicated that the novel...

Tài liệu Báo cáo khoa học: An alternative isomerohydrolase in the retinal Muller cells of a cone-dominant species doc

... thetase to esterify the 13cROL generated in the reac-tion. Therefore, 13cROL is accumulated in the absenceof LRAT in the reaction (Fig. S3B).Under the in vitro assay conditions of the ... the entire thicknessof the inner retina. The Mu¨ller cell constitutes an ana-tomical link between the retinal neurones and supportstheir activities by exchanging molecules between the other ... from the reaction were analyzed byHPLC. In the absence of a metal chelator, RPE65c cat-alyzed the production of 11cROL and 13cROL from atRE (Fig. 4A). However, in the presence of 1 mM ofthe...

Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

... mitochondria during apoptosis. They induce the permeabilization of the mitochondrial membranewith the release of cytochrome c and the formation of the apoptosome [38]. The major pro-apoptotic proteinBax ... mitochondriaand then translocate to the nucleus where they induce DNA fragmentation in a caspase-independent manner. Whether apoptosis is inducedor not depends on the activation balance of these proteins. ... in the cytosol [59], whereasCAD ⁄ ICAD is activated in the nucleus [14]. Duringapoptosis, both caspases are activated and caspase-3,but not caspase-7, translocates from the cytosol intothe...

Tài liệu Báo cáo khoa học: Basis of recognition between the NarJ chaperone and the N-terminus of the NarG subunit from Escherichia coli nitrate reductase pdf

... enthalpy is the sum of twoterms: the reaction enthalpy, independent of the bufferused in the experiment, and another term representing the contribution of the proton ionization of the buffer,which ... HCl, 1 mM MgCl2, 100 mM NaCl. The upper panels show the raw data for the heat effect during the titrations; the lowerpanels are the binding isotherms.Table 2. Thermodynamic parameters of NarG(1–15) ... hypothesize that the proton-ated state of the chaperone initiates this cycle, whereas the deprotonated state occurs upon completion of the maturation process of the partner. The nature of the signal...

Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt

... signal within the GBD part of the protein. Another possible explanation for the observedautoinhibitory activity is that the Hth domain mediatessome intramolecular interaction, or affects the confor-mation ... 1D). To furtherdeﬁne the Meis2d transcriptional AD, we tested twoother GBD fusion proteins, which contained either the Meis2 HD and C-terminal region, or just the regionC-terminal to the HD. ... that the protein encoded by the Meis2e splice variant has alimited ability to act as an effective dominant negative. The Hth domain inhibits the activity of a linkedADTo further delineate the...

Tài liệu Báo cáo khoa học: Steﬁn A displaces the occluding loop of cathepsin B only by as much as required to bind to the active site cleft doc

... A from the com-plex with cathepsin H are shown in cyan (PDB code: 1NB3) [28]. The two structures of stefin A from the complex with cathepsin Bare shown in red. The stefin B structure from the ... residues, A49 from the tip of the first binding loop and L73 from the second binding loop, fill the places that the two histi-dines occupy in the native structure. Besides the lasso, the inhibitor ... direc-tions. The side chain of H110 points away from the active site cleft to the back of the molecule, whereas the side chain of H111 points upwards and away from the surface. In the complex,...

Tài liệu Báo cáo khoa học: Perturbation of membranes by the amyloid b-peptide – a molecular dynamics study pptx

... Val24 at the membrane–water inter-face, the other with Lys28 at the interface [4,5]. The location of the peptide within the membrane mayaffect the types of interactions that it has with the surrounding ... distance from the protein, for simulationA1. The results are indicative of all the other simulations involving Ab, and thus are representative of the general conclusions discussed in the text. There ... proposedby the authors of the original melittin simulations inwhich the peptide was embedded in the membrane[18]. They observed that the surrounding lipids tendto pack around the peptide and tilt their...

Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf

... 296(154) [14–17]. The high speciﬁc activity of theseFVIIa variants is presumably linked to a more stableburial of the N-terminus of the protease domain in the activation pocket of the activation ... were based on the results of the titra-tion experiment.Enzymatic activity, inhibitor reactivity and sTFbinding of G372A-FVIIa The very slow auto-activation of G372A-FVII, orrather the need to ... slightlysubstrate-dependent. The fact that the relative reduc-tion in activity was not greater in the presence ofcofactor strongly suggests that the allosteric mecha-nism behind the TF-induced activity enhancement...

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