Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): a unique metalloprotein ppt

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Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): structure–function relationships docx

... including: a pre-served array of 20 cysteine residues; and two domains,each of which wrap around a metal–thiolate clusterKeywordsmetallothionein (MT); mutation; neuronal growth-inhibitory factor ... themetal–thiolate cluster, and the domain–domain interactions.AbbreviationsAD, Alzheimer’s disease; DTNB, 5,5¢-dithiobis-(1-nitrobenzoic acid); GIF, neuronal growth-inhibitory factor; hGIF, human ... notexhibit any neuronal growth-inhibitory activity, it doesplay an important role in modulating the stability ofthe metal–thiolate cluster and conformation ofthe b-domain by domain–domain interactions,...

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): reactivity and structure of metal–thiolate clusters* doc

... S, Saito T, Hosokawa T, Takahashi Y,Numata T & Kurasaki M (2005) Metal binding abilityof metallothionein-3 expressed in Escherichia coli. BasicClin Pharmacol Toxicol 96, 295–301.36 Karotki ... Palumaa P, Eriste E, Njunkova O, Pokras L, JornvallH & Sillard R (2002) Brain-speciﬁc metallothionein-3has higher metal-binding capacity than ubiquitousmetallothioneins and binds metals ... 76–80.27 Faller P, Hasler DW, Zerbe O, Klauser S, Winge DR &Vasak M (1999) Evidence for a dynamic structure ofhuman neuronal growth inhibitory factor and for majorrearrangements of its metal-thiolate...

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): evaluation of the biological function of growth-inhibitory factor in the injured and neurodegenerative brain pdf

... Kohmura E, Sakaki T, Nonaka M,Yamada K, Yamashita T, Kishiguchi T, Sakaguchi T &Hayakawa T (1997) Expression of growth inhibitory factor mRNA after focal ischemia in rat brain. J CerebBlood ... impaired. MolChem Neuropathol 32, 101–121.17 Sogawa CA, Asanuma M, Sogawa N, Miyazaki I,Nakanishi T, Furuta H & Ogawa N (2001) Localiza-tion, regulation, and function of metallothionein-III ⁄growth ... orneurodegenerative brain.Discovery of GIFAD is a neurodegenerative disease that leads to severedementia and ultimately death. The pathologicalhallmarks of the disease are intracellular neuroﬁbril-lary...

Tài liệu Báo cáo khoa học: Computational approaches to understand a-conotoxin interactions at neuronal nicotinic receptors doc

... Torpedo marmorataray and availability of selective pharmacological tools likethe snake a- neurotoxins. The analysis of both muscle and neuronal subunit sequences reveal a high degree of identityand ... remarkable features. We havegenerated homology models of several nAChR subtypes[2 7a] , so that we can start to analyse in detail the nAChRpharmacophore that binds a- conotoxins at the molecularlevel. ... bindingdomain), four transmembrane domains, an intracellularloop and an extracellular C-terminus. a4 /b2 Receptors,which mainly control pain [7], and a7 receptors are themost abundant nAChR subtypes...

Tài liệu Báo cáo khoa học: Structure-activity relationships of a-conotoxins targeting neuronal nicotinic acetylcholine receptors ppt

... Queensland, Brisbane, QLD, Australia a- Conotoxins that target the neuronal nicotinic acetylcho-line receptor have a range of potential therapeutic applica-tions and are valuable probes for examining ... Mutational analyses havealso been valuable in elucidating factors important foractivity. Table 2 lists the most informative mutations thathave been made in a range of a- conotoxins. In addition,an ... (2-amino-D,L-dodecanoic acid, Laa) to eitherthe N-terminus (LaaMII) or to Asn5 (5LaaMII). TheN-terminal LaaMII was shown to have a tertiary structuresimilar to that of the native conotoxin and maintained...

Tài liệu Báo cáo khoa học: C-Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism doc

... CT ex114 aa208 aa24 aaBamH34 aaStartStopXho1N-Pro Protease domainProtease domainCT - ex380 aa1IINP114 aa208 aaBamH134 aaStartStopXho1SS SSGSSSSN-Pro356 aaIIIMSTLFIISILLFLASFSYAMDISTIEYKYDKSSAWRTDEEVKEIYELWLAKHDKVYSGLVEYEKRFEIFKDNLKFIDEHNSENHTYKMGLTPYTDLTNEEFQAIYLGTRSDTIHRLKRTINISERYAYEAGDNLPEQIDWRKKGAVTPVKNQGKCGSCWAFSTVSTVESINQIRTGNLISLSEQQLVDCNKKNHGCKGGAFVYAYQYIIDNGGIDTEANYPYKAVQGPCRAAKKVVRIDGYKGVPHCNENALKKAVASQPSVVAIDASSKQFQHYKSGIFSGPCGTKLNHGVVIVGYWKDYWIVRNSWGRYWGEQGYIRMKRVGGCGLCGIARLPYYPTKAAGDENSKLETPELLQWSEEAFPLAIV A B66 ... protease from the latex of Ervatamia coronaria.Biosci Biotechnol Biochem 62, 1947–1955.15 GuhaThakurta P, Biswas S, Chakrabarti C, Sundd M,Jagannadham MV & Dattagupta JK (2004) Structuralbasis ... proteases [Erv -A, -B and –C (isolated from thelatex of Ervatamia coronaria in our laboratory) and papainfrom Carica papaya (Merck, Kenilworth, NJ, USA)], twoserine proteases [trypsin and chymotrypsin...

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... (excluding water moleculesand peptide atoms) and rjis the standard deviation of Bfactors. The normalized B factors have a zero mean and unitvariance. All atoms that satisfy Bz‡ 4 are treated as ... importin -a nuclear import pathway, provided that CLIC4 can undergo a conforma-tional rearrangement that exposes the NLS in an extended conformation.DatabaseStructural data are available in ... importin -a: CLIC4 NLS peptide interaction.NLS residueInteractions a Van der Waals’ contact area (A ˚2)bBuried surfacearea (A ˚2) RSCCcMain chain Side chain Main chain Side chain TotalVal201...

Tài liệu Báo cáo khoa học: Kinetics of dextran-independent a-(1 ﬁ 3)-glucan synthesis by Streptococcus sobrinus glucosyltransferase I pdf

... Methyl a- d-glucopyranoside was used as a glucose analog that is not a substrate of hexokinase in a glucose assay system. The data were analyzed on thebasis of the Michaelis–Menten equation; the maximumactivity ... glucansucrasemodulates glucan and oligosaccharide synthesis. ApplEnviron Microbiol 66, 1923–1927.27 Komatsu H, Katayama M, Sawada M, Hirata Y, MoriM, Inoue T, Fukui K, Fukada H & Kodama T (2007)Thermodynamics ... weights of activated carbon (Wako PureChemical, Osaka, Japan) and Celite (Wako Pure Chemical)(Fig. S4). The column was maintained at 15 °C, and wasthen washed with water until the glucose was completelyeluted....

Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a coupled oscillator-based mechanism in smooth muscle ppt

... Pharmacology, University of Calgary, Alberta, Canada2 School of Biomedical Sciences, University of Newcastle, Callaghan, NSW, AustraliaLong-range signalingBiological organs display coordinated ... membrane depolarization activating oradvancing the phase of other Ca2+stores. The electricaland chemical transduction pathways are as depicted inFig. 3. The key mechanisms are as follows: (a) ... relative change in ﬂuorescencenormalized to baseline. (B) Slow wavesrecorded at two sites 6 mm apart along a strip before, during and after central applica-tion of 1 mM caffeine applied at...

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