... 50S 19 11 1 917 19 11 1 917 U1 911 C – + – +A1 912 U + + ND NDA1 912 C + + ND NDA1 913 G + + + +C1 914 U + + + +C1 914 A + + + +U1 915 C + + + +A1 916 G + + – –A1 916 C + + + +A1 916 U – – – –U1 917 C + – + –A1 918 G ... gene and 17 7 nt from the clpB gene.Preparation of ribosomes and rRNA23S rRNA variants A1 912 U, A1 912 C, A1 913 G, C1 914 U,A1 916 G, A1 916 C, A1 916 U, A1 917 G, A1 919 C, and A1 919 1U were expressed in the ... containing the E. coli23S rRNA gene with streptavidine binding tag [29]. Mutant23S rRNA variants A1 912 U, A1 912 C, A1 913 G, C1 914 U,A1 916 G, A1 916 C, A1 916 U, A1 917 G, A1 919 C, and A1 919 1U were fused to ptBB...