Tài liệu Báo cáo khoa học: Membrane targeting and pore formation by the type III secretion system translocon pdf

... their cognate chaperones and are injected through the translocon pore and into the targetcytoplasm. IM, inner membrane; MO, outer membrane. P J. Matteı¨et al. Membrane targeting and pore formation ... Journal 278 (2011) 414–426 ª 2010 The Authors Journal compilation ª 2010 FEBSREVIEW ARTICLE Membrane targeting and pore formation by the type III secretion system translocon Pierre-Jean Matteı¨1, ... stoichiometry of pore formation, membrane targeting and the potential rolethat the translocon can play in the regulation of secre-tion. In addition, little structural information regarding the hydrophobic...

Tài liệu Báo cáo khoa học: High-afﬁnity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

... this type exhibit the predictedamount of binding only if the heterodimer is tethered by the wild -type partner. By contrast, we found that, if the heterodimer is tethered to the resin by the mutantpartner, ... on the functionof the contralateral binding site. The mutant site doesnot prevent the wild -type site from binding IGF-II and pairing with a wild -type subunit does not repair the defect in the ... 4 °C. The lines in each graph (F, G) indicate the amount of binding predicted if the wild -type and mutant receptorsare binding ligand independently. The tables indicate the amountsof the various...

Tài liệu Báo cáo khoa học: Olfactory receptor signaling is regulated by the post-synaptic density 95, Drosophila discs large, zona-occludens 1 (PDZ) scaffold multi-PDZ domain protein 1 pptx

... detect and discriminate between thou-sands of different odorant molecules is governed by the diverse array ofolfactory receptors expressed by olfactory sensory neurons in the nasalepithelium. ... further depolarizing the cell. How these diversesignaling molecules ﬁnd each other in the complex and densely-packed environment of the cell, avoidingcross-talk with other signaling pathways, ... zona-occludens 1 (PDZ) domains, has the ability to bindto various molecules in the signal transduction cas-cade, thereby bringing them into close proximity and ensuring a rapid and speciﬁc signal transduction[9,12].PDZ...

Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf

... betweenJ(0) and J(xN) and between J(0) and J(0.87xH) for the TTF-1 HD were then examined as proposed by Lefe`-vre et al. [21]. The ﬁt was obtained by linearregression, and only the corresponding ... interruption of the geometryof the recognition helix for either Antp or the TTF-1HD, the anomalous amide exchange pattern and the NOE connectivity data of the C-terminal portion ofhelix III had to ... 8.41–16.53 s)1. The lowest R2values are shown by the N-terminal and C-terminal residues and by Leu34,Gln44, Arg53, Arg58 and Gln59. A unique value of26.39 s)1, by far the highest one,...

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... 2008 The Authors Journal compilation ª 2008 FEBS 4391Zn-binding motif and the two disulﬁde bonds respec-tively connecting the a4-helix to the b4 strand and the b9 strand to the b10 strand at the ... concentrationof 3.4 lm.Thermophilicity and thermostabilityThermophilicity was evaluated in the temperature range40–90 °C by measuring the polymerase activity of the Rep245 under standard conditions ... of 0.2 nm, and a bandwidth of1 nm. Thermal stability of Rep245 was measured by incu-bating the protein at 60, 70 and 80 °C for 30 min and thenrecording the CD spectra of the incubated samples...

Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx

... [49], and aCHO-derived cell line [50]. However, the site at which the PI3K signaling feeds into the Erk activating path-way varies in these systems: at the Ras, Raf or MEKactivation level. In the ... and required for the for-mation of epithelia in the embryo and the maintenanceof the polarized epithelial structure in the adult [2]. Asa single-span transmembrane-domain glycoprotein,E-cadherin ... disruption of the epitheliallayer (Fig. 1A, B,D) and acquisition of a scatteredphenotype (Fig. 1B). In addition, E-cadherin disap-peared from the plasma membrane and was redistri-buted into the cytoplasm...

Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

... in the association of the translationalcomplex with the microsomal membrane [13,14]. Thus, the N-terminal hydrophobic sequences of CYPs areimportant for their targeting to and retention in the ER ... targeting of CYPs to the ER and mitochondria, Alzheimer’samyloid precursor protein to the plasma membrane and mitochondria, and translocation of the cytosolicglutathione S-transferases to the mitochondrial ... min on ice, and both the sol-uble and insoluble fractions were separated by centrifugation(Labnet, Hermlez-233M, 220.59 rotor); the samples wereprocessed as described by Anandatheerthavarada...

Tài liệu Báo cáo khoa học: Specific targeting of a DNA-alkylating reagent to mitochondria Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide doc

... within the cyto-plasm driven by the plasma membrane potential and thenbe further accumulated within the mitochondria due to the mitochondrial membrane potential [11]. From the knownplasma and ... driven by theplasmamembranepotential(Dwp) followed by the further accu-mulation of mitoDC-81 into mitochondria driven by the mitoch-ondrial membrane potential (Dwm) is illustrated. The Nernst ... gel and the ﬂuorescence recorded (A and C). The DNA wasthen transferred to a nylon membrane and TPP moieties covalentlyattached to the DNA were visualized using anti-TPP serum (B and D).The...

Tài liệu Báo cáo khoa học: Membrane orientation of laminin binding protein An extracellular matrix bridging molecule of Leishmania donovani pdf

... lactoperoxidase-glucose oxidase. Membrane proteinswere then isolated by biotinylation and streptavidin/agaroseextraction, analysed by SDS/PAGE and autoradiographed. The 67-kDa LBP, together with other membrane proteinswere ... in both the trypsinreleased supernatant and the cell pellet. In other words, both the 27-kDa fragment of LBP released by trypsin as well as the 34-kDa fragment retained in the cell membrane ... lysosome, where theydifferentiate, these parasites have to surpass the formidablebarrier of the ECM and BM. The ability to adhere to ECMcomponents may represent a mechanism by which the pathogen...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... of the heme groups of the enzyme by various compounds was tested to identify the physiologicalelectron acceptor of the enzyme. The enzyme was notoxidized by the heterodisulﬁde of coenzyme M and coenzyme ... with the other subunitsof the enzyme complex.From the primary structure, the b -type cytochrome-likeprotein AF501 and subunit AF500 are clearly predicted tobe integral membrane proteins. The ... of the Hme complex and characterization by UV/Vis spectroscopyTo determine whether the cytochrome present in the Hmecomplex is reduced by menaquinone, in vitro assays wereperformed using the...

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