Tài liệu Báo cáo khoa học: PCR detection of nearly any dengue virus strain using a highly sensitive primer ‘cocktail’ ppt

... 4 ATATGCTGAAACGCGTGAGCATCATGAGACAGAGCGATDENV-3 1043822791G5P30 Group 5 TTCCAACAAGCAGAACAACATGCTACAGGCAGCACGGTTTDENV-4 990310318415C. Gijavanekar et al. PCR detection of nearly any dengue ... CAGACTAGTGGTTAGAGGAGAGGAATGATGCTGTAGAGACADENV-1 92.1 ± 0.57 73.6 ± 2.311G4P217 ATATGCTGAAACGCGTGAGCATCATGAGACAGAGCGATDENV-3 90.3 ± 1.09 83.9 ± 5.161G5P30 TTCCAACAAGCAGAACAACATGCTACAGGCAGCACGGTTTDENV-4 81.8 ... formulation and demonstration of a mixed primer PCR reagent that may enable the detection of nearly any dengue strain irrespective of serotype, in a single PCR reaction, and illustrate anapproach...

Tài liệu Báo cáo khoa học: "Automatic Detection of Nonreferential It in Spoken Multi-Party Dialog" doc

... shallow feature generation meth-ods could propagate into the model that waslearned from the data. The advantage of this ap-proach is, however, that training and test data arehomogeneous. A ... of the classnonreferential. The overall classiﬁcation accuracywas 75.1%.The advantage of using a machine learning sys-4http://www.cs.waikato.ac.nz/ ml/tem that produces human-readable models ... precision of 82% and a recall of 71% using a memory-basedmachine learning approach, and a similar preci-sion but much lower recall (42%) using a decisiontree classiﬁer.In summary, the best approaches...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... 5¢-AAGCTTGAGCGGATCCCCAGCGCGCAACCACC-3¢ and5¢-GCAGCAGGATCCTCTAGAGAGTTTAGTCTTTG-3¢ for FLAG-DEC1; and 5 ¢-GAATTCGGCGGACCAGAGAATGGACATTTCCTCAACCATC-3¢ and5¢-TCTAGACTACAGCGGCCATGGCAAGTCACTAAAGTC-3¢ ... 5¢-AAGCTTCACCATGTACCCTGCCCACATGTACCAAGTGTAC-3¢,5¢-AAGCTTCACCATGCCGCACCGG CTCATCGAGAAAAAGAG-3¢,5¢-AAGCTTCACCATGGCAGTGGTTCTTGAACTTACCTTGAAGC-3¢ or5¢-AAGCTTCACCATGATTGCCCTGCAGAGTGGTTTACAAGCTG-3¢) were used. Ampliﬁed ... ¢-TCTAGACTAGGAGCTGATCAGGTCACTGCTAGTGAAATGG-3¢,5¢-TCTAGACTACCCACTCGAGTGAGCGAAAGTCCGCTGG-3¢ or 5¢-TCTAGACTATTGACCTGTTTCGACATTTCTCCCTGACAGCTC-3¢)wereusedfor PCR ampliﬁcation, with hDec1 cDNA as a template.For...

Tài liệu Báo cáo khoa học: "Reﬁned Lexicon Models for Statistical Machine Translation using a Maximum Entropy Approach" pptx

... Statistical Machine Translation using a Maximum Entropy ApproachIsmael Garc´ a VareaDpto. de Inform´aticaUniv. de Castilla-La ManchaCampus Universitario s/n02071 Albacete, Spainivarea@info-ab.uclm.esFranz ... number of occurrences that a spe-ciﬁc feature must appear. So a cut-off of 0 meansthat all features observed in the training data areused. A cut-off of 32 means those features thatappear 32 ... Statistical Machine TranslationThe goal of the translation process in statisti-cal machine translation can be formulated as fol-lows: A source language stringis to be translated into a target...

Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx

... (5¢-TAGTTCCCCGGGGGTGAAGCTGAAGTTTCTCTGACCGGTCAGCCGTTC-3¢) and reverse primer tsf-DOWNS (5¢-AGTCAGGATCCGTCGACAGAGCTTCGCCACTCAACTTAAGCAGAA-3¢). Like primer Ts185, Ts224 contains a unique AvaI restriction site(underlined) and part of a sequence ... ensure a satisfactory frequency of homologous recombination for the gene replacement. Theupstream fragment was prepared using forward primer tsf-UPS (5¢-ATGCGGGATCCAAGCTTGAGCTTACATCAGTAAGTGACCGGGATGA-3¢) ... was inserted as alinker (Figs 1 and 2).Chromosomal DNA from E. coli strain UY211 (ara,D(lac-pro), nalA, thi) [32] was isolated and used as a templatein two separate PCR reactions to prepare...

Tài liệu Báo cáo khoa học: "The impact of language models and loss functions on repair disﬂuency detection" pptx

... the repair often contains many of the same words that appear in the reparandum. Infact, in our Switchboard training corpus we foundthat 62reparandum also appeared in the associatedrepair,to ... noisy-channel model. We show that language mod-els trained on large amounts of non-speechdata improve performance more than a lan-guage model trained on a more modest amount of speech data, and ... a similar formula.9 ResultsWe follow Charniak and Johnson (2001) and splitthe corpus into main training data, held-out train-ing data and test data as follows: main training con-sisted of...

Tài liệu Báo cáo khoa học: "Automatic Extraction of Lexico-Syntactic Patterns for Detection of Negation and Speculation Scopes" pdf

... LinguisticsAutomatic Extraction of Lexico-Syntactic Patterns for Detection of Negationand Speculation ScopesEmilia ApostolovaDePaul UniversityChicago, IL USAemilia.aposto@gmail.comNoriko TomuroDePaul ... state -of- the-art machinelearning systems. Additionally, the intuitiveand linguistically motivated rules will allowfor manual adaptation of the rule set to newdomains and corpora.1 MotivationInformation ... (exact match).6 Related WorkInterest in the task of identifying negation and spec-ulation scopes has developed in recent years. Rele-vant research was facilitated by the appearance of a publicly...

Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

... lyase pathway). Bottom: cleavage toyield AMPA and glyoxylate (the AMPApathway), referred to as the GOX pathway.(B) Reaction catalyzed by GO on glyphosate,an alternative to the AMPA pathway ... glyphosate cannotbe regarded a mere analog of PEP, but it ratherappears to mimic an intermediate state of PEP, pre-sumably that of the elusive carbocation. More than1000 analogs of glyphosate have ... different from that of GOX.OxidasesGOX (Monsanto)Early on, Monsanto Co. isolated glyphosate-AMPAbacteria from a glyphosate waste stream treatmentfacility. Achromobacter sp. LBAA was thus identiﬁedfor...

Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt

... Pervandate and catalase wereprepared as described previously [25]. Brieﬂy, vanadatestock solution was prepared as a 200 mM solution of sodium orthovanadate (pH 10). Pervanadate was preparedas ... blotting of lysates from pervanadate-treatedcells with an antibody against total tau revealeddecreased electrophoretic mobility of tau, with theappearance of an  68-kDa tau species in wild-type andall ... increasedamount of tau bound to Fyn-SH2, as compared withcells treated with catalase (Fig. 1A) . Furthermore, a tau species of  68 kDa was apparent in SH2 pull-downs from pervanadate-treated...

Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... ascomycete fungal laccase fromThielavia arenaria – common structural features of asco-laccasesJuha P. Kallio1, Chiara Gasparetti2, Martina Andberg2, Harry Boer2, Anu Koivula2, Kristiina Kruus2,Juha ... observed for MaL, that may determinethe properties of these asco-laccases at high protein concentrations.DatabaseStructural data are available in the Protein Data Bank database under the accession ... copper-containing enzymes used in various applications, suchas textile bleaching. Several crystal structures of laccases from fungi andbacteria are available, but ascomycete types of fungal laccases...

Xem thêm