... 2011 FEBS 2295
Crystal structure of an ascomycete fungal laccase from
Thielavia arenaria – common structural features of
asco-laccases
Juha P. Kallio
1
, Chiara Gasparetti
2
, Martina Andberg
2
, ... from the analogous structures
of the ascomycete laccase (asco -laccase) MaL, thus giv-
ing a comprehensive view of the structure and function
of a...
... was transformed into BL21 (DE3) cells and grown at
37 °C overnight in Luria–Bertani medium containing
30 lgÆmL
)1
of kanamycin. Flasks of 400 mL of Luria–
Bertani medium (30 lgÆmL
)1
kanamycin) ... factor of
atom i, l
j
is the mean B factor (excluding water molecules
and peptide atoms) and r
j
is the standard deviation of B
factors. The normalized B factors have a zero mean and unit...
... Three of the ligands,
OD2 of Asp165, NE2 of His79 and NE2 of His169,
formed an equatorial plane. The other protein ligand,
NE2 of His31, bound to the metal, in the company of
a water oxygen, from ... Suzuki
5
and Yasushi Kawata
3,4
1 National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan
2 Department of Food Science and Nutrition, Faculty of...
... 516
Number of protein molecules 2 4
Number of water molecules 804 181
Number of glycerol molecules 2 –
Number of sodium ions 4 –
Number of magnesium ions 1 –
Number of sulfate ions – 16
Mean B factor ... Umbach P
(2003) Facilities and methods for the high-throughput
crystal structural analysis of human proteins. Acc Chem
Res 36, 15 7–1 63.
K. Bo
¨
hm et al....
... domain. Structure 11 , 112 3–1 131.
8 Lu DS, Futterer K, Korolev S, Zheng XL, Tan K,
Waksman G & Sadler JE (1999) Crystal structure of
enteropeptidase light chain complexed with an analog
of the ... (2007) 214 8–2 160 ª 2007 Max-Planck-Insitute of Biochemistry Journal compilation ª 2007 FEBS
Crystal structure of the catalytic domain of DESC1, a new
member of th...
... an a-helical hairpin
(helices a5, a6) and a C-terminal strand (b8). Helix a5
and strand b7 partially cover one side of the Ross-
mann fold structure, thereby interacting with helices
a1, a2 and ... (2007) 304 4–3 054 ª 2007 The Authors Journal compilation ª 2007 FEBS 3045
mixed, three-stranded b-sheets, each one consisting of
the antiparallel b-strands b6, b7 of one monomer and
str...
... 272 (2005) 83 2–8 45 ª 2005 FEBS
structures, with a cut-off distance of 3.5 A
˚
showed that
8 5–9 3% of the Ca-atoms lie at common positions and
gave a root mean square deviation of 0.8 4–1 .21 A
˚
(Table ... Arg and Lys) within a dis-
tance of 4 A
˚
. The meso- and psychrophilic structures
have the same number of salt-bridges and only two
fewer than the thermophilic struct...
... 34, 1497 5–1 4986.
23. Due
´
e, E., Olivier-Deyris, L., Fanchon, E., Corbier, C., Branlant,
G. & Dideberg, O. (1996) Comparison of the structures of wild-
type and a N313T mutant of Escherichia ... mechanistic approach and the design of
inhibitors such as 1,3-BPGA analogues far from easy.
Indeed, the most potent and selective inhibitors of
gGAPDH from parasites (T. brucei,...
... fold revealed
by comparision of the crystal structures of transketolase, pyruvate
oxidase and pyruvate decarboxylase. Structure 1, 9 5–1 03.
Ó FEBS 2003 3D structure of indolepyruvate decarboxylase ... [40]. An alternative model is based on
the structure of ScPDC with bound activator pyruvamide,
which revealed a disorder–order transition of two active
site loops (residue...
... of intrinsically dynamic behav-
ior. These regions correspond to D11–E14, A46–R51,
G53–C57, K83–G89, S101–A104, D117–N119 and
G122–F125.
Description of hirsutellin A structure
The structure of ... The
structure contains an a-helix (a
1
, V21–A31), a single
helix turn (a
2
, N56–D58) and seven b-strands (b
1
, I 3–
C6; b
2
, F17–D20; b
3
, H42–Y44; b
4
, L64–P68; b
5
, R9 5–...