... suggestingthat TS participates in the LPS-stimulated signal pathwayleading to NO production. Protein kinase C (PKC)inhibitors, but not protein kinase A inhibitors, inhibited the enhancing effect ... NOproduction, indicating that TS activated a LPS-stimulatedsignal pathway (Fig. 4). PKC is a key kinase in the LPS-stimulated signal pathway [30,31]. The findings that PKCinhibitors, not PKA inhibitors, ... bath-typesonicator for 5min. The amount of protein of the solubilizedcells was determined with a bicinchoninic acid protein assaykit (PIERCE, Rockford, IL, USA) using bovine serumalbumin as a standard....