... Utrecht,Department of Pulmonary Diseases, G 035 50, Heidelberglaan 100, 35 84 CX Utrecht, the Netherlands.Fax: + 31 30 2505414, Phone: + 31 30 2507 134 ,E-mail: P.Coffer@hli.azu.nlAbbreviations: PtdIns3K, phosphatidylinositol -3 ... J. Biochem. 269, 4625–4 634 (2002) Ó FEBS 2002 doi:10.1046/j.1 432 -1 033 .2002. 031 62.xhydrolyses PtdIns(4,5)P2into diacylglycerol and InsP 3 .InsP 3 initiates the release of Ca2+from the endoplasmaticreticulum, ... asdescribedinMaterialsandmethods.(A)ERK-P and p38 MAPK-P. (B) PKB-PSer4 73, PKB-P Thr308 and GSK3-P. Thelevels of ERK2, p38 MAPK and actin weredetermined as a control of equal loading. Dataarerepresentativeofatleastthreeindependentexperiments....