... ions in the latter way, the experimenter should have examined the number of ligands, the geometry of the coordination sphere, andthe B-factor of the ion and its environment For example, the distance ... the PDB website for each deposited structure It may be a good habit to have a look at the atomic coordinate section of the PDB file to see the level of the B-factors of the atoms that are in the ... on the constellation of all atoms within the unit cell In other words, the crystal structure is encoded in the diffracted X-rays – the shape and symmetry of the cell define the directions of the...
... day The proportion of cells resistant to puromycin in the sample was determined fromthe equation This was performed for each day on which data were available for the sample, andthe proportion ... amount of the plasmid, and 72.5 µl of CaCl2 was mixed together The mix was swirled, filter sterilised and allowed to stand for 20 minutes at room temperature for the DNA to precipitate The mix ... Different vector preparations were used to transduce Jurkat T cells In each experiment, transduction was conducted using both the neat vector stock and a 1/10 dilution of the preparation The proportion...
... Government of India, and senior consultant tothe World Bank l Herbert Kubicek, director of the Institute for Information Management Bremen and scientific director of the Digital Opportunities Foundation, ... measure and how to determine progress The US and Russia: Access does not always equal more use In the US, for example, despite the rise in the proportion of the population using computers andthe Internet, ... around the world The Finnish model is one of open competition and privatesector-led development with political support place for competition andthe private sector will seize the opportunity to make...
... in the selection of the particular meaning we from dictionaries, novels, short stories andtheInternet Since the use to understand a linguistic expression” whole research work relied on the corpora, ... confused using the WDBs both English and Vietnamese Clarifying the semantic features will help learners of English and Vietnamese to understand and use them effectively in order to achieve their ... by the WDBs that once the WDBs are used, the speaker andthe hearer must have This thesis has made a study of the semantic and pragmatic the same image or meaning of these words If we both have...
... for investigation of the catalytic mechanism and substrate specificity andto understand the differences therein for this class of enzymes In order to identify the subsites of the ervatamins and ... maintained together in either the test set orthe working set Although ˚ the data could be processed up to 2.5 A, there were some practical problems in using the full dataset in the refinement – the ... this class of enzymes On the other hand, the Ser32Oc of ervata428 min-C pointing away fromthe helix forms a hydrogen bond with Arg172Ne fromthe other domain, and helps to form an intricate interdomain...
... strand andthe b9 strand tothe b10 strand at the bottom of the Zn-stem loop in the pRN1 prim–pol structure However, because the Zn-stem loop is a fairly self-standing structure protruding from ... for the content or functionality of any supporting information supplied by the authors Any queries (other than missing material) should be directed tothe corresponding author for the article FEBS ... replication protein comprising the N-terminal amino acids 1–245 and then including the residues predicted to be responsible for the DNA polymerase and primase activities, accordingly tothe homology modeling...
... processing According tothe results of previously reported molecular and structural studies the processing of the b-amylases from sweet potato [3,38] and soybean [39,40] also is restricted tothe ... inhibitor (Ki ¼ 262 mM ) when compared to maltose (Ki ¼ 11.7 mM ) and cyclohexaamylose (Ki ¼ 0.36 mM ) Northern blot analysis was performed to determine the total length of the mRNA encoding the ... endodermis and rhizodermis (Fig 6) In cross-section of cells of the pith andthe cortex, the vacuole is the dominant organelle The cytoplasm is visible only as a thin layer adjacent tothe cell...
... binding onto the active site From these results, taken together, we conclude that the Glu526 side-chain can adopt two conformers (A-form and B-form) in the substrate-bound form, and Asp524 acts to restrain ... around v1 relative tothe A-form and was exposed tothe solvent The two oxygen atoms of the Glu526 side-chain in the A-form were positioned close tothe proximal glycosidic oxygen atom (O1) at dis˚ ... in the D524A–substrate complex, an altered conformation of the Glu526 side-chain (B-form) was observed in addition tothe favorable conformer for proton transfer (A-form) (Fig 3C) In the B-form,...
... that correspond to helix and helix of the second EcCM protomer; in *YpCM, the disulfide bond is between the third residue of the mature protomer andthe bottom of helix of the second EcCM protomer, ... gray andthe other is rose In 90-MtCM, one polypeptide chain is blue andthe other is green The TSA fromthe EcCM structure is shown with yellow carbon atoms in the 90-MtCM structure for orientation ... with the TSA onto 90-MtCM to see the TSA in the active site of 90-MtCM (Figs and 6) As the residues corresponding to Ser84 and Gln88 of EcCM are absent in 90-MtCM, chorismate is unlikely to be...
... components into the cytosol, where the A components gain access toand interact with their cellular target or targets to cause toxic effects on the host cell For a large family of A–B toxins, the intracellular ... G-protein-coupled receptor; G12 ⁄ 13PCR, G12 ⁄ 13-protein-coupled receptor; GiPCR, Gi-protein-coupled receptor; GqPCR, Gq-protein-coupled receptor; Grb2, growth factor receptor-bound adaptor protein 2; ... phosphatidylinositol-dependent phospholipase Cb isoform; PPAR, peroxisome-proliferator activated receptor; Pref1, preadipocyte factor 1; Rac, Rac1 small regulatory GTPase; Raf, Ras-activated factor serine...
... Phenol, phenol red, and DB 53 were found to be adsorbed strongly on organoclays comprising alternating organic and inorganic layers Adsorption behavior of the three adsorbate–adsorbent systems was ... in original bentonite Then, these organic cations exist in ‘‘liquid form’’ [30] and they have affinity toward organic substances It can be observed that increasing pH leads to a decrease in adsorption ... 37.81 37.93 To characterize adsorption of different phenolic compounds using organoclays, the Langmuir adsorption isotherm was employed The linear form of Langmuir adsorption isotherm equation...
... Tory Health The Cavalier The Lamentation Of A Bad Market, OrThe Disbanded Souldier The Courtier's Health; Or, The Merry Boys Of The Times The Loyal Tories' Delight; Or A Pill For Fanaticks The ... Scotland and Ireland England and America are too prosaic and too busy, andthe masses, notwithstanding all their supposed advantages in education, are much too vulgar to delight in either song or ... appealed tothe passion, if not tothe intellect of the masses, and instructed them in all the leading events of the time In our day the people need no information of the kind, for they procure it from...
... principle for us today Today, sales to commercial and public customers account for approximately 80 percent of revenue and there’s nothing more direct than using the input we get from social media to ... used Dell.com to buy products Today, it’s the focal point for a variety of social platforms where they learn from each other as well as from us Tomorrow, the boundaries between online and offline ... about tools, people, and organization To get the greatest impact, you need to introduce the technology to a receptive audience and then apply it for business value We’ve organized this book into...
... that isn’t the point The more specific you are, the better you will perform on keywords that matter to you andthe people you want to reach Although the search volume for those keywords may not ... constituents such as customers, regulators, and government agencies requires vision, commitment, and a tolerance for error Not all companies have the culture or fortitude to make the shift They are better ... Marketing tothe Business Customer much more if you expand your criteria The information you get back is only as good as the keywords you search Among the search phrases to consider are: Competitors...
... population They range from just a few hundred wordsto many thousands The best policies are more than just rules; they guide employees on how to use social channels tothe greatest benefit for the organization ... foods, Monsanto wanted a public place to tell its story Rather than having to respond to individual queries from reporters, Monsanto linked to its argument against the need for labeling These are ... guidance fromthe community Marketers start looking to customers to help them with positioning andto seek help evangelizing the company to others Active communities literally change companies from the...
... with brand aspirations “If they see themselves as the low-cost leader, it’s going to be tough to get them to search optimize for a keyword like ‘cheap,’” said Greg Jarboe, the father of the search ... Relevant keywords are terms and phrases that your customers use when they’re looking for the products or services you offer But sometimes, the keywords customers search are distasteful to marketers ... written in a more informal tone, so as not to compete with the more formal messaging on the corporate website,” says Odden And in the blog, you might create a post that’s an argument for embracing...
... with others to joining groups to posting status updates, is captured in the profile The more active the member is, the higher the visibility andthe greater the value of the network to his or her ... some support people to monitor the posts on the forum andto resolve them quickly I posted on the blog what progress we were making We had an advisory board of 80 customers, and they were more than ... while other topics may peter out Consider moving the active threads into their own area This makes it easier for members to find others with similar interests and also for you to monitor the ebb and...
... followers, andthe like When they launched the 2011 Ford Explorer, the Ford Motor Company ran online display ads giving users the choice to click through to a Facebook Page or a destination landing ... According to Scott Monty, the automaker’s digital and multimedia communications manager, unique visitors coming fromthe Facebook page were 30 percent more likely to take the intended on the landing ... acknowledging all the while that features may not work the way they’re intended These companies are learning, in thewords of author Mike Moran, to Do It Wrong Quickly They understand that customers can...