3 1 label retention assay components a thymidine molecule a dna nucleoside 3htdr 3h thymidine a thymidine analog used in the original assay for marking the dividing cells brdu 5 bromo 2′ deoxyruidine and edu 5 ethynyl 2´ deoxyur
THE SPATIOTEMPORAL STUDY OF ZEBRAFISH INTESTINAL EPITHELIUM RENEWAL
Ngày tải lên :
10/09/2015, 09:25
... original assay for marking the dividing cells; BrdU (5- Bromo- 2 deoxyruidine) ; and EdU (5- ethynyl- 2´- deoxyuridine): the other thymidine analog (B) Click-iT EdU vs anti -BrdU antibody staining of thymidine ... DAPI Scale bar = 20 µm 53 XIII Figure -1: Label retention assay components: (A) thymidine molecule: a DNA nucleoside; 3HTdR ( [3H ]thymidine) : a thymidine analog used in the original ... (Ishizuya-Oka, 2007) Figure 1- 8: Intestinal epithelium renewal in adult amphibian and mammalian intestine Similar to the mammalian intestine, the epithelial cells in amphibian intestine undergoes...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
Ngày tải lên :
12/08/2014, 11:23
... Corporation) EdU (5- ethynyl- 2’-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis One milligram of EdU in 10 0 μl of PBS was injected intraperitoneally ... study and to manuscript preparation All authors contributed to interpretation of the data All authors read and approved the final manuscript Page 10 of 11 18 19 20 Competing interests The authors ... MSCs were analyzed at passages 3, 4, and (a) and IFN-gR KO MSCs were analyzed at passages and 12 (b) for expression of CD11b, CD 45, and Sca -1 Likewise, other phenotypic markers were analyzed on...
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Retrovirology Research BioMed Central Open Access In vivo expression of the HBZ gene of HTLV-1 doc
Ngày tải lên :
12/08/2014, 23:20
... sclerosis: an expanded disability status scale (EDSS) Neurology 19 83, 33 :14 44 -14 52 Kamihira S, Sugahara K, Tsuruda K, Minami S, Uemura A, Akamatsu N, Nagai H, Murata K, Hasegawa H, Hirakata Y, Takasaki ... 2008, 5: 34 Murata K, Hayashibara T, Sugahara K, Uemura A, Yamaguchi T, Harasawa H, Hasegawa H, Tsuruda K, Okazaki T, Koji T, Miyanishi T, Yamada Y, Kamihira S: A novel alternative splicing isoform ... Proviral loadc HBZ mRNA/DNAd tax mRNA/DNAe C5/MJ HUT102 MT1 MT2 MT4 SLB1 13 .3 1. 2 25. 2 7.8 2.4 25. 8 0.062 26. 35 0. 011 1. 24 1. 71 87.4 8 .1 19.3 7 .1 16.2 12 .6 11 5. 5 1. 64 0.063 3 .56 0.48 0 .19 0.22...
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Báo cáo y học: " The connection domain in reverse transcriptase facilitates the in vivo annealing of tRNALys3 to HIV-1 genomic RNA" potx
Ngày tải lên :
13/08/2014, 13:20
... supported by a grant from the Canadian Institutes for Health Research We thank Y Huang and G Nabel for the gift of plasmid hGagPol∆FS∆PR 20 References 21 10 11 12 13 14 15 Jiang M, Mak J, Ladha A, Cohen ... P27 5 6.P ∆4 P67 ∆4 1. P ∆4 BH 10 P- C - Gag 10 0 75 50 25 Figure The incorporation of GagPol and tRNALys3into wild-type and mutant HIV -1 The incorporation of GagPol and tRNALys3into wild-type and ... including the thumb domain of RT are deleted ( 5 81 and ∆ 7 15 ) To measure the amount of tRNALys3 annealed in vivo to the viral RNA genome, total viral RNA was used as the source of primer/template in an...
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Báo cáo sinh học: "A dual function fusion protein of Herpes simplex virus type 1 thymidine kinase and firefly luciferase for noninvasive in vivo imaging of gene therapy in malignant glioma" ppsx
Ngày tải lên :
14/08/2014, 19:22
... and Therapy 2004, 2:7 10 11 12 13 14 15 16 17 18 19 20 21 22 Shand N, Weber F, Mariani L, Bernstein M, Gianella-Borradori A, Long Z, Sorensen AG, Barbier N: A phase – clinical trial of gene therapy ... experiments and enzymatic assays SJ and AS carried out the immunohistochemical studies NGR and AS designed the experiments and evaluated the data All authors have read and approved the manuscript Acknowledgements ... 19 99, 10 :23 25- 23 35 Rainov NG: A phase III clinical evaluation of herpes simplex virus type thymidine kinase and ganciclovir gene therapy as an adjuvant to surgical resection and radiation in adults...
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Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier
Ngày tải lên :
23/04/2013, 21:38
... lung, and kidney were fixed in 10 % phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo ... 1. 0 (Anhui, China) program Bioavailability (BA) is a measurement of the rate and extent of a therapeutically active drug that reaches the systemic circulation and is available at the site of action ... Sex n Day Day Day 15 18 .46 ± 0. 81 23 .12 ± 1. 51 24.90 ± 1. 04 Control 18 .64 ± 1. 17 25. 68 ± 2. 81 30.02 ± 2. 25 18 .14 ± 0.68 22. 85 ± 1. 12 18 .68 ± 0. 51 26.30 ± 0.80 30.06 ± 1. 48 Table 4. Food intake...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
Ngày tải lên :
22/02/2014, 07:20
... Transformants were incubated h at 37 °C and overnight at room temperature in SOC medium and then plated on Luria–Bertani agar plates containing kanamycin Kanamycin resistant (KmR) transformants ... membranes were then stained for 0 .5 1. 0 in 0.04% Coomassie R 250 (prepared in a solution of 50 % methanol and 10 % acetic acid) Destaining was carried out in 50 % methanol for 5 10 followed by an extensive ... °C Samples of cells were withdrawn at 10 –30 intervals and after measuring the D 550 value, cells were lysed by SDS-containing solution the relative amount of UP12 in the samples and was estimated,...
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Báo cáo khoa học: In vivo production of catalase containing haem analogues pot
Ngày tải lên :
06/03/2014, 11:20
... Fe-PP-KatA (Fig 3A, B) (maxima at 430, 54 4 and 57 7 nm for Co-PP-KatA, at 420, 5 51 and 59 0 nm for Sn-PP-KatA, at 4 21, 55 4, 57 4, 629 and 670 nm for Zn-PP-KatA, and at 404, 52 6, 55 9 and 677 nm for Ru-meso-KatA) ... catalases are presented in Fig 3A, B Fe-PP-KatA showed a Soret peak at 406 nm and weak absorption bands at 50 4, 5 41 and 6 25 nm These features are characteristic for haemcontaining catalases [17 ] ... haem analogues can be inserted into the protein The catalase proteins were isolated by the same procedure as used for Fe-PP-KatA and Ga-PP-KatA The resulting preparations were pure or contained...
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Báo cáo khoa học: A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae ppt
Ngày tải lên :
07/03/2014, 16:20
... [Gal80p]max [Gal3p]max 2.0 · 10 )10 M 0.4 1. 0 1. 0 · 10 )7 M 1. 0 · 10 )10 M 5. 0 · 10 )11 M 6.3 · 10 )11 M 1. 0 · 10 )10 M 30 0 .5 · 2.372 · 10 )11 · 2.372 · 10 )11 5. 47 · 10 )9 M 1. 0 · 10 )6 M 5. 0 · 10 )6 M M M ... [2–4 ,12 ,17 ,19 ] The input–output relationships were mainly quantified by assuming interactions at equilibrium and applying molar balances for the different components Thermodynamic parameters and ... kap 114 p and a possible mechanism of intrecellular targeting J Cell Biol 14 5, 14 07 14 17 16 Komeili, A & O’Shea, E.K (2000) Nuclear transport and transcription Curr Opin Cell Biol 12 , 355 –360 17 ...
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Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx
Ngày tải lên :
07/03/2014, 21:20
... 0. 75 are shown Black geometrical shapes are additional domains, as indicated ANOGA, Anopheles gambiae; ASHGOS, Ashbya gossypii; BRARE, Brachydanio rerio; CANDGLA, Candida glabrata; CIONA, Ciona ... constitute the minimal transcriptionally active fragment and are required simultaneously to maintain transcription The PHF3 protein was recovered in initial analyses and also contains a TFS2M domain ... distribution (Fig 1) , in which the SPP1 representatives are at the basal branch of CGBP and DIO The GCBP proteins contain a PHD domain, followed by a DNA- binding domain (the zf-CXXC) and the newly described...
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Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx
Ngày tải lên :
07/03/2014, 21:20
... the administered [75Se]Der p2, indicate together with its own remaining intact levels (the 16 kDa band) that the clearance and further metabolism of the allergen was altered as a result of the in ammation ... the Sel-tag had an intact core sequence and maintained allergen-specific IgE-binding epitopes and the use of a Sel-tag enabled labelling with the gamma-emitting radionuclide 75Se at a single predefined ... (Cobra II Auto-Gamma, Packard Instrument Company) The protein concentrations in the supernatants were thereafter determined by the Bradford protein assay (Bio-Rad, Hercules, CA, USA) using BSA as...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
Ngày tải lên :
07/03/2014, 21:20
... underlined) or 5 -TGGAGCCACCCGCAGTTCG AAAAAGAAGCTGGAGATGATCGTGCT-3¢ (Strep-tag underlined) and 5 -GCGGCATGCTCATCCAAAGAAGC TTGGGTCG-3¢ The two amplified fragments were used as a combined template for ... underlined) or 5 -TGGAGCCACCCGCAGTTCGAAAAAGAA GCTGGAGATGATCGTGCT-3¢ (Strep-tag underlined), then a secondary amplification with primers 5 -CACCAC CACCACCACCACGAAGCTGGAGATGATCGTGCT-3¢ (His-tag underlined) ... the control of the 35S promoter and terminator For each tagged variant and the wild-type PSAG, a cassette containing the PSAG construct flanked by the 35S promoter and terminator was excised and...
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Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis pot
Ngày tải lên :
08/03/2014, 08:20
... p21WAF1/CIF1: 5 -ACAGCGATATCGAGACACTCA-3¢ (forward) and 5 -GTGAGACACCAGAGTGCAAGA-3¢ (reverse); primers for p53: 5 -CACAGTCGGATATGAGCATC-3¢ (forward) and 5 -GTCGTCCAGATACTCAGCAT-3¢ (reverse) and primers for ... primers for cyclin D1: 5 -TGTTCGTGGC CTCTAAGATGA-3¢ (forward) and 5 -GCTTGACTCCA GAAGGGCTT-3¢ (reverse); primers for 18 S rRNA: 5 -GAGTATGGTCGCAAGGCTGAA-3¢ (forward) and 5 -GCCTCCAGCTTCCCTACACTT-3¢ (reverse) ... kb had 10 0% homology to part c c Hypervitaminosis 1. 61 ± 0 .12 (5) 1. 60 ± 0.22 (5) b 78.08 ± 15 .40 (3) a 4276 ± 13 34 (5) a 44 .14 ± 6. 05 (5) a 262.74 ± 35. 55 (5) a of the rat c-H-Ras cDNA and hybridized...
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Báo cáo Y học: In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis docx
Ngày tải lên :
08/03/2014, 22:20
... 99, 95 10 0 Serrano, R (19 83) In vivo glucose activation of the yeast plasma membrane ATPase FEBS Lett 15 6, 11 14 Eraso, P & Gancedo, C (19 87) Activation of yeast plasma membrane ATPase by acid ... Acta 11 51 , 83–88 ´ Rosa, M & Sa-Correia, I (19 91) In vivo activation by ethanol of plasma membrane ATPase of Saccharomyces cerevisiae App Env Microbiol 57 , 830–8 35 ´ Fernandes, A & Sa-Correia, ... temperatures [11 ] In this case too, changes in the H+-ATPase activity followed increases in the unsaturation of plasma membrane fatty acids, in particular, linolenic acid Furthermore, revisiting these...
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Báo cáo khoa học: A study of microRNAs in silico and in vivo: bioimaging of microRNA biogenesis and regulation doc
Ngày tải lên :
16/03/2014, 01:20
... developmental stages and the changes of these networks in disease and after application of a variety of therapeutic strategies Critically, 217 2 the noninvasive imaging approach of miRNA generation and ... non-invasive molecular imaging information regarding how and when they are transcribed by transcription factors and where they originate, in the case of a miRNA located on multiple chromosomal ... Also, miRNA 21 is a potential therapeutic 217 0 target for cancer treatment, as overexpression of antimiRNA 21 in hepatocellular carcinoma and glioblastoma cells was shown to down-regulate the oncogenic...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
Ngày tải lên :
30/03/2014, 04:20
... calpain, calpastatin, was preferentially converted into still active 15 kDa fragments (Table 1) , whereas, in aorta, the inhibitor was predominantly inactivated As both the inactivation and fragmentation ... Averna et al Table Levels of native and 15 kDa calpastatin species in brain and aorta of NMS and HMS rats treated with HSD for weeks The data reported are the arithmetical means ± standard deviation ... degradation of NOS and HSP90 by calpain higher than in aorta, resulting in a much higher HSP90 to NOS ratio in brain (Fig 1C) A Calpain activation in rat brain and aorta following HSD treatment...
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báo cáo hóa học:" In vivo properties of the proangiogenic peptide QK" pdf
Ngày tải lên :
18/06/2014, 15:20
... applicable A p value less than 0. 05 was considered to be significant All the statistical analysis and the evaluation of data were performed using GraphPad Prism version 5. 01 (GraphPad Software, San ... USA 20 05, 10 2 :14 2 15 -14 220 Dudar GK, D'Andrea LD, Di Stasi R, Pedone C, Wallace JL: A vascular endothelial growth factor mimetic accelerates gastric ulcer healing in an iNOS-dependent manner Am ... Circulation 20 01, 10 4 :11 5 -11 9 Karpanen T, Bry M, Ollila HM, Seppanen-Laakso T, Liimatta E, Leskinen H, Kivela R, Helkamaa T, Merentie M, Jeltsch M, Paavonen K, Andersson LC, Mervaala E, Hassinen...
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Báo cáo hóa học: " In vivo evidence of htid suppressive activity on ErbB-2 in breast cancers over expressing the receptor" potx
Ngày tải lên :
18/06/2014, 16:20
... 1, 20 1, 88 -0,24 -0 ,19 0,87 -0,66 0,69 0, 41 0,44 0,27 1, 18 1, 14 0 ,55 1, 58 69 41 44 27 11 8 11 4 55 15 8 14 ,55 15 , 61 15, 34 15 ,84 13 ,39 13 ,43 14 , 75 14 ,22 ΔΔCt -0,4 0,67 0,4 0,90 -1, 55 -1, 51 -0 ,19 -0,72 ... 2,08 0 ,59 1, 56 2 ,13 0,93 0,83 38 208 59 15 6 213 93 83 15 , 91 13,02 13 ,90 13 ,84 13 ,32 17 ,55 13 , 51 0,97 -1, 92 -1, 04 -1, 10 -1, 62 2, 61 -1, 43 0, 51 3,80 2,06 2 ,14 3,07 0 ,16 2,70 51 380 206 214 307 16 270 ... sample loading and the amount of input cDNA Amplification of HGPRT was performed using the primers 5' TGA CAC TGG CAA AAC AAT GCA-3' (sense) and 5' GGT CCT TTT CAC CAG CAA GCT-3' (antisense) at...
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báo cáo hóa học:" Function of anterior talofibular and calcaneofibular ligaments during in-vivo motion of the ankle joint complex" docx
Ngày tải lên :
20/06/2014, 01:20
... which investigated the changes in ATFL and CFL strain in response to loading in various positions Colville et al. [10 ] measured strain in lateral ankle ligaments in 10 cadaveric ankles while applying ... Stability of the ankle joint Analysis of the function and traumatology of the ankle ligaments Acta Orthop Scand Suppl 19 85, 211 :1- 75 Rasmussen O, Andersen K: Ligament function and joint stability ... nation (SU), maximal pronation measured neutral position Lengths the ATFL and CFL as(PR) and at at maximal supiLengths of the ATFL and CFL as measured at maximal supination (SU), maximal pronation...
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báo cáo hóa học:" In vivo evaluation of a vibration analysis technique for the per-operative monitoring of the fixation of hip prostheses" pdf
Ngày tải lên :
20/06/2014, 01:20
... stages and canal and reinserting the prosthesis The FRF had a normal evolution during the reinsertion and the graphs corresponding to the final two stages, labelled as stage 4a and stage 5a, are ... were simulated in terms of contact areas and interface stresses, and the vibration response in each step was calculated by finite elements analysis Building on the understanding and clinical experience ... deformed endomedullary canal as the FRF analysis showed an abnormality and the surgeon was alerted to the situation in time during insertion of the stem (Figures 7a c) The supplementary information...
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