ф 29 dna dependent dna polymerase
Tài liệu Báo cáo khoa học: Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase d and S6K1 and regulating cell growth docx
... the polymerase (DNA- directed), delta interacting protein (POLDIP3) gene as a binding partner of the human ER PDIP46 ⁄ SKAR had been reported to be an interacting partner of the p50 subunit of DNA ... molecular partners of the p50 subunit of DNA polymerase d [23] No further data on this interaction and the possible role of PDIP46 in processes in which this polymerase takes part have been reported ... with no direct influence on the metabolism of pyrimidines Experimental procedures cDNA cloning and DNA constructs cDNAs of the human genes, ER and POLDIP3 (both isoforms) corresponding to their coding...
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Tài liệu Báo cáo khoa học: Dissociation of DNA polymerase a-primase complex during meiosis in Coprinus cinereus pptx
... with either p140 cDNA (upper panel), p48 cDNA (middle panel), or glyceraldehyde 3-phosphate dehydrogenase (G3PDH) cDNA (lower panel) Ó FEBS 2003 Meiotic expression of Coprinus DNA polymerase a (Eur ... Coprinus DNA polymerase a (Eur J Biochem 270) 2143 Fig Separation of primase activity from pol a-primase complex by phospho–cellulose column chromatography (A) DNA polymerase (circles) and DNA primase ... should be noted that intrinsic DNA polymerase activity in fraction II has quite low processivity for DNA synthesis which is a typical feature for replicative DNA polymerases [1] Taken together...
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Báo cáo khoa học: The N-terminal region of the bacterial DNA polymerase PolC features a pair of domains, both distantly related to domain V of the DNA polymerase III s subunit ppt
... level, DnaE and PolC have many features in common Both have a similar polymerase core consisting of ‘palm’, ‘thumb’ and ‘fingers’ domains The polymerase core in both DnaE and PolC is flanked by a polymerase ... major double-stranded DNA binding determinant in the E coli DnaE [12] Crystal structures revealed that this motif binds double-stranded DNA similarly in both PolC [8] and DnaE [6] The b-clamp ... PolC and DnaE The ability to bind single-stranded DNA has indeed been demonstrated for the E coli DnaE OB domain [12,16] The very N-terminal region of PolC and the C-terminal domain of DnaE appear...
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Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
... polynucleotide kinase, E coli DNA polymerase I, the Klenow fragment of E coli DNA polymerase I lacking 3¢ to 5¢ exonuclease activity, Taq DNA polymerase and pfu DNA polymerase were from Takara ... DNA polymerase and DNA polymerase I) degrading the product To our surprise, the addition of doxorubincin did not obviously inhibit expansion, but did inhibit the exonuclease activity of DNA polymerase ... Klenow fragment (Fig 3B, lane 2), Sequenase Version 2.0 DNA poly4514 merase (Fig 3C, lane 2), Taq DNA polymerase (Fig 3E, lane 2) and pfu DNA polymerase (Fig 3F, lane 2) The addition of DDI-1A and...
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Báo cáo khoa học: DNA polymerase e associates with the elongating form of RNA polymerase II and nascent transcripts pot
... associates with RNA polymerase II 24 25 26 27 28 29 30 31 32 33 34 35 36 37 (1996) DNA polymerase e may be dispensable for SV40- but not cellular -DNA replication EMBO J 15, 2298 –2305 Pospiech H, ... compilation ª 2006 FEBS 5537 DNA polymerase e associates with RNA polymerase II A K Rytkonen et al ¨ A B C Fig DNA polymerase (Pol) e interacts with hyperphosphorylated RNA polymerase (pol) II (A) Pol ... 2006 FEBS A K Rytkonen et al ¨ A B Fig UV crosslinking of replicative DNA polymerases to nascent DNA or RNA chains (A) DNA polymerases (Pols) a and e, but not Pol d, are photolabelled with radioactive...
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Báo cáo khoa học: Plant DNA polymerase k, a DNA repair enzyme that functions in plant meristematic and meiotic tissues docx
... other X-family DNA polymerases, a tree was drawn based on the amino acid sequences of the DNA polymerase domains Ó FEBS 2004 Plant DNA polymerase k (Eur J Biochem 271) 2803 Table DNA polymerization ... eukaryotic DNA polymerases of the X-family Fig DNA polymerase activity of OsPol k protein (A) Multiple amino acid alignment of a portion of the polymerization active site with the other X-family DNA polymerases ... effects of DNA polymerase inhibitors on the DNA polymerase activity of OsPol k Strong inhibition was noted with 2,3-dideoxythymidine-5-triphosphate, a known inhibitor of mammalian DNA polymerases...
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Báo cáo khoa học: The noncatalytic C-terminus of AtPOLK Y-family DNA polymerase affects synthesis fidelity, mismatch extension and translesion replication ppt
... AtPOLK DNA polymerase and displacement of the replicative DNA polymerase [3,11] Despite its important in vivo functions, the role of the C-terminal region of Y-family DNA polymerases during DNA ... amino acids stimulates the DNA polymerase activity and processivity of AtPOLK on different DNA substrates The C-terminally truncated AtPOLK DNA polymerase is not affected in DNA binding We next examined ... C-terminus in AtPOLK DNA polymerase replicative DNA polymerases preferentially insert A opposite 8-oxodG [21], causing G:C fi T:A transversions [31] Insertion preferences among Y-family DNA polymerases...
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Báo cáo khóa học: Crystal structure of the chi:psi subassembly of the Escherichia coli DNA polymerase clamp-loader complex doc
... 54 54 24374933 26987715 23104211 2299 4951 21230123 30248459 23028461 33593407 2299 8318 15677419 2298 6355 34498368 2297 6686 23016368 2296 4979 16125938 2296 8178 2295 9552 17987543 23108152 39 33 30 ... holoenzyme DnaX complex: association of dd¢ with DnaX (4) forms DnaX (3) dd¢ EMBO J 19, 6536–6545 Tsuchihashi, Z & Kornberg, A (1990) Translational frameshifting generates the c subunit of DNA polymerase ... on DNA – a three-point switch underlies primer handoff from primase to the replicative DNA polymerase Cell 96, 153– 163 27 Xiao, H., Crombie, R., Dong, Z., Onrust, R & O’Donnell, M (1993) DNA polymerase...
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Báo cáo khoa học: Initiation of JC virus DNA replication in vitro by human and mouse DNA polymerase a-primase ppt
... origin of DNA replication (B) The JCV system uses JCV TAg and pJC389 DNA with the JCV origin The cell extracts were supplemented with 0.5 and 1.0 DNA polymerase units of the indicated DNA polymerase ... previously, SV40 DNA replication is absolutely dependent upon human DNA polymerase a-primase (Fig 1A, columns 1–5 [26,27]) and more specifically, upon the human p180 subunit as the hybrid DNA polymerase ... Biochem 270) 2033 Fig DNA synthesis products of the SV40 and JCV DNA replication systems Murine FM3A cell extracts depleted of DNA polymerase a-primase were supplemented with 1.0 DNA polymerase units...
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Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot
... catalytic activities: (a) DNA polymerase activity, which can be both RNA and DNA dependent; and (b) RNase H activity that selectively hydrolyzes the RNA strand of the RNA :DNA hybrid formed during ... TRP229A Tyr188 O O O LEU234A O O NNRTIbp PHE227A O TYR188A Trp 229 PRO236A Trp 229 Pr Tyr181 TYR188A Cys181 VAL106A TRP229A LEU100A C G LEU100A TYR188A K54 TYR318A O Asp110 K54 TYR183A Trp 229 O ... VAL108A Asp186 O O O Asp185 O NNRTIbp Asp185 TYR318A TRP229A Trp 229 TYR188A MET230A Tyr188 O Tyr181 O Trp 229 TYR188A O O O O NNRTIbp TRP229A LEU234A Cys181 LEU100A Br TYR181A B F Asp110 VAL108A...
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Báo cáo khoa học: A dideoxynucleotide-sensitive DNA polymerase activity characterized from endoreduplicating cells of mungbean (Vigna radiata L.) during ontogeny of cotyledons pptx
... ddNTP-sensitive DNA polymerase activity Fig Comparative analysis of the N-terminal heptatide sequence of purified mungbean DNA polymerase with the other X-family DNA polymerases: (A) DNA polymerase ... for dTTP of mungbean DNA polymerase was 0 .29 lm (Fig 4C), close to the value of 0.3 lm of rice DNA polymerase for dTTP [19] and human polymerase b (0.33 lm) for UV-induced DNA damage repair [24] ... DNA polymerase was 2.3 lm (Fig 4D), slightly higher than value for ddTTP of the ddNTP-sensitive DNA polymerase from rice and of human DNA polymerase b (< 2.0 lm), but much less than that of DNA...
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Báo cáo khoa học: Distinctive activities of DNA polymerases during human DNA replication ppt
... ¨ (1996) DNA polymerase e may be dispensable for SV40- but not cellular -DNA replication EMBO J 15, 2298 –2305 12 Pospiech H & Syvaoja J (2003) DNA polymerase epsi¨ lon – more than a polymerase ... Journal 273 (2006) 298 4–3001 ª 2006 The Authors Journal compilation ª 2006 FEBS 299 1 Human DNA polymerases a, d and e in replication A K Rytkonen et al ¨ Table Distribution of DNA polymerases (pols) ... FEBS Journal 273 (2006) 298 4–3001 ª 2006 The Authors Journal compilation ª 2006 FEBS 299 3 Human DNA polymerases a, d and e in replication A K Rytkonen et al ¨ Fig DNA polymerase (pol) d and pol...
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Báo cáo khoa học: Sulfoquinovosylmonoacylglycerol inhibitory mode analysis of rat DNA polymerase b pdf
... MA DNA polymerase activity (%) A N Kasai et al 10 Fig (A) Doseresponse curve of SQG Rat DNA polymerase b (0.05 units) was preincubated with the indicated concentrations (0100 mM) of SQG DNA polymerase ... of DNA polymerase beta Correlation with DNA binding and dRP lyase activity J Mol Biol 296 , 2292 53 18 Sawaya MR, Prasad R, Wilson SH, Kraut J & Pelletier H (1997) Crystal structures of human DNA ... SQMG(C18:1)) and NA on the activities of DNA polymerase b, as a function of the DNA template-primer and the nucleotide substrate concentrations Rat DNA polymerase b was 0.05 units Substrate conc...
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Báo cáo Y học: The DNA-polymerase inhibiting activity of poly(b-L-malic acid) in nuclear extract during the cell cycle of Physarum polycephalum pot
... with the recovery of > 95% of DNA polymerase a and > 75% of the other DNA polymerases in the extract Standard DNA polymerase activity and inhibition assays Total DNA polymerase activity was assayed ... attached to DNA polymerases was an indicator of the polymerase activity and was measured after SDS/PAGE by autoradiography Separate results are shown in Fig 2C for DNA polymerase e and for DNA polymerases ... the total nuclear DNA polymerase activity in the standard assay Results by SDS/ PAGE and Western blotting with specific antisera against DNA polymerases a and e [13], and DNA polymerase d [14]...
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Báo cáo hóa học: "DNA polymeraseh protein expression predicts treatment response and survival of metastatic gastric adenocarcinoma patients treated with oxaliplatin-based chemotherapy" pot
... cisplatin GpG adducts by human DNA polymerase eta Biochemistry 2000, 39:4575-80 Matsuda T, Bebenek K, Masutani C, Hanaoka F, Kunkel TA: Low fidelity DNA synthesis by human DNA polymerase eta Nature 2000, ... translesion DNA synthesis (TLS) [20] These adduct repairs occur primarily through NER [21] TLS is another alternative way to repair these lesions, which is mainly done by DNA polymerases h [22] Polymeraseh(Polh), ... polh in tumor cells and poor Discussion The present study partially revealed the role of DNA polymeraseh as a DNA repair protein in gastric cancer by detecting its expression in four gastric cancer...
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Báo cáo hóa học: " A broadly applicable method to characterize large DNA viruses and adenoviruses based on the DNA polymerase gene" doc
... products but the vertebrate DNA polymerase genes contain introns making the DNA polymerase products from the host genomic DNA much larger than from viral genomic DNA The predicted PCR product ... isolates representing the most common DNA viruses of vertebrates that contain DNA polymerase genes: herpesviridae, iridoviridae, poxviridae and adenoviridae The DNA polymerase PCR was performed on ... disease Also, the amino acid sequences of DNA polymerase of many large DNA viruses have been compared to evaluate virus relationships and compared to the DNA polymerases of other organisms to hypothesize...
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Báo cáo khoa học: "The ORF59 DNA polymerase processivity factor homologs of Old World primate RV2 rhadinoviruses are highly conserved nuclear antigens expressed in differentiated epithelium in infected macaques" pps
... origin dependent DNA synthesis, including an origin binding protein, a helicase, a primase, a primase-associated factor, a single-stranded DNA binding protein, a DNA polymerase, and a DNA polymerase ... highly conserved DNA polymerase in open reading frame (ORF) and a DNA polymerase processivity factor in ORF59 [9] KSHV ORF59 binds DNA as a homodimer, interacts with the ORF9 DNA polymerase to strongly ... as well as a region that enhances the processivity of the DNA polymerase [6] Transport of the viral DNA polymerase into the nucleus is dependent on ORF59 [20], which contains a nuclear localization...
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Báo cáo y học: " A nucleotide binding rectification Brownian ratchet model for translocation of Y-family DNA polymerases Ping Xie" pot
... in Y-family DNA polymerases and their implications for polymerase function and lesion bypass DNA Repair (Amst) 2002, 1:343-358 75 Glick E, Vigna KL, Loeb LA: Mutations in human DNA polymerase ... (at least) two substeps, E* · DNA · PPi ® E · DNA · PPi ® E · DNA + PPi, where the transition from the activated E* · DNA · PPi ternary complex to unactivated E · DNA · PPi ternary complex results ... the DNA substrate and PPi Since in the activated E* · DNA · dNTP (or E* · DNA · PPi) complex the Pol has a stronger interaction with DNA substrate and nucleotide than in the unactivated E · DNA...
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Nghiên cứu đặc tính sinh học phân tử virus dịch tả vịt tại Việt Nam qua khảo sát các gen UL5, UL32 và DNA polymerase
... cỏc base ca RNA v DNA Cỏc mi ny c tng hp nh hot ng ca mt loi enzyme khỏc, gi l primase Tt c DNA- polymerase u tng hp DNA theo hng t u 5' n u 3' ca DNA Cho n khụng cú enzyme DNA- polymersae no cú ... phỏp gii trỡnh t (sequencing) da vo hot ng ca enzyme DNA- polymerase quỏ trỡnh tng hp DNA, emzym DNA- polymerase xỳc tỏc gn cỏc nucleotide vo mch n DNA ang tng hp v trớ cú cha nhúm -OH t do, gp nucleotide ... Ging 1: sn phm PCR nhõn gen DNA- polymerase ca chng DTVCKN; ging 2: sn phm PCR nhõn gen DNA- polymerase ca chng DTVVXKN cú kớch thc khong 0,5 kb; ging M: thang DNA chun (DNA ca thc khun th c ct bng...
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