β structured fibrous proteins and self assembling peptides

Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt

Ngày tải lên : 18/02/2014, 16:20

... of peptides and proteins N D Udeshi et al dimethylation of Arg, monomethylation, dimethylation and trimethylation of Lys, acetylation and ubiquitination of Lys, and phosphorylation of Ser and ... Hart [11], this modiﬁcation is found on proteins in both the nucleus and cytoplasm, is added and removed by transferase and O-GlcNAcase enzymes, respectively, and often occurs at sites that are known ... reactions and tandem MS ETD has most recently been utilized for the direct analysis of intact proteins, and ubiquitin is a model protein that was initially interrogated via ETD for tandem MS Shown...

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mass spectrometry of proteins and peptides

Ngày tải lên : 11/04/2014, 09:50

... and Kent, B H (1992) Weighing naked proteins: practical, highaccuracy mass measurement of peptides and proteins Science 257, 1885–1894 Pedrocchi, M., Schafer, B W., Durussel, I., Cox, J A., and ... Direct Analysis of Proteins in Mixtures 17 Direct Analysis of Proteins in Mixtures Application to Protein Complexes John R Yates, III, Andrew J Link, and David Schieltz Introduction Tandem mass spectrometry ... Winston, S., and Hauer, C R (1986) Protein sequencing by tandem mass spectrometry Proc Natl Acad Sci USA 83, 6233–8238 Yost, R A and Boyd, R K (1990) Tandem mass spectrometry: quadrupole and hybrid...

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báo cáo khoa học: "Self-assembly of proteins and their nucleic acids" docx

Ngày tải lên : 11/08/2014, 00:21

... biotinylated SAPV84 DNA and precipitated by anti-BCMP84 antibody Self- assembly of protein vectors with their DNAs and affinity separation Co-transcriptional and co-translational self- assembly of SAPV ... by joining the untagged SAPV and modified AFP by PCR using primers T7-F and TFTER-R and a mixture of the SAPV fragment (M13F/SA-11R PCR fragment) and of the linear and modified AFP fragment (AFP-1F/SA-8R ... gel and band intensities determined using GeneSnap and fluorescent imager from SynGene The results of measurements are presented on Figure Self- assembly of protein vectors with their DNAs and...

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Báo cáo y học: "Effects of p-Synephrine alone and in Combination with Selected Bioflavonoids on Resting Metabolism, Blood Pressure, Heart Rate and Self-Reported Mood Changes"

Ngày tải lên : 25/10/2012, 11:04

... and serve as antioxidants and anti-inflammatory agents [12, 13] as well as hepatoprotectants [14] and neuroprotectants [15] However, the effects of naringin and hesperidin on metabolic rate and ... participants completed a second self report rating scale After 75 min., a third and final self report rating scale was completed, and measurements of blood pressure, heart-rate and RMR were determined ... hesperidin and 600 mg naringin Group 4: Advantra Z® with 100 mg hesperidin and 600 mg naringin Group 5: Advantra Z® with 1,000 mg hesperidin and 600 mg naringin After remaining seated and resting...

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Top-Ten Success and Self Improvement Tips

Ngày tải lên : 18/10/2013, 00:15

... a tool back in its exact spot, not having spare printer and computer media The trick is to be tougher on yourself to be kinder to yourself in the longterm • Breaking Down Unpleasant Jobs into ... who is always tired • Be Early Train yourself to be early/on time for everything business and pleasure, you’ll get the best welcome, have chance to meet and analyse the others as they arrive • ... going! • Positive Thinking Only accept positive views on life, people and projects Stop reading gloomy newspapers etc, try and mix with positive go-getters Choose success • Improve your Decision...

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Personal identity and self-knowledge

Ngày tải lên : 01/11/2013, 07:20

... braintransplantation and related procedures, see Sydney Shoemaker, Self- Knowledge and Self- Identity (Ithaca, NY: Cornell University Press, 1963), pp 22ff and Bernard Williams, ‘The Self and the Future’, ... from both philosophical and psychological perspectives, see Jose ´ Luis Bermudez, Anthony Marcel and Naomi Eilan (eds.), The Body and the Self ´ Personal identity and self- knowledge 271 it refer ... University Press, 1990) and John Campbell, Past, Space, and Self (Cambridge, MA: MIT Press, 1994), ch and ch For wide-ranging further discussion of the concept and development of selfconsciousness,...

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Tài liệu Marketing Food to Children and Adolescents - A Review of Industry Expenditures, Activities, and Self-Regulation docx

Ngày tải lên : 18/02/2014, 07:20

... beverages and candy/frozen desserts ($11.6 million each), and juice and non-carbonated beverages ($10.2 million) Relative to overall in-store and packaging expenditures for the reported brands, the ... appeared on packages and store displays for fruit snacks, breakfast cereals, candy, yogurt, frozen waffles, toaster pastries, macaroni and cheese, and fruits and vegetables Multi-brand food companies ... baked goods, and fruits and vegetables Cross-promotions targeting an adolescent audience were used for snack foods, candy, and carbonated drinks ES-3 Marketing Food to Children and Adolescents...

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Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx

Ngày tải lên : 19/02/2014, 05:20

... analysis of ligand binding to C-LytA (A) Conformational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands: s, d, 2.5 and 20 mM choline; ... 10 Experimental conditions depended on the enzyme, and were set as follows: 37 °C and pH 6.9 for LytA; 30 °C and pH 6.0 for LytC; and 30 °C and pH 6.9 for Pce The specific activities of the enzymes ... single-step purification system for CBPs and CBM-containing fusion proteins ([26], and C-LYTAG Purification System User’s Manual from Biomedal, http://www.biomedal.es), and gave support to the hypothesis...

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Tài liệu Báo cáo khoa học: The small heat shock proteins and their role in human disease pptx

Ngày tải lên : 19/02/2014, 18:20

... Y Sun and T H MacRae recognition and initiating internal signaling cascades Many peptides generated by degradation of self and nonself bind HSPs noncovalently, indicating cells of origin and cause ... potential and decrease cell protection by Hsp25 In another example, Hsp27 and aB-crystallin appear in Parkinson’s disease 2617 Small heat shock proteins and disease with severe dementia [119] sHSPs and ... HSPs that protect deregulated intracellular signaling proteins and transcription factors 2619 Small heat shock proteins and disease Y Sun and T H MacRae Table sHSP modiﬁcations associated with...

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Báo cáo khoa học: Functional classiﬁcation of scaffold proteins and related molecules pptx

Ngày tải lên : 06/03/2014, 22:21

... Functional classiﬁcation of scaffold proteins L Buday and P Tompa Table Scaffold proteins and their kin Representatives of the four categories of scaffold proteins and their relatives are presented ... functional description of scaffold proteins and their kin, in general, and their distinct classes, in particular, encompassing scaffolds [5], docking proteins [6], anchors [7] and adaptors (this article) ... regulating more proteins in complex ways (scaffolds ⁄ anchoring proteins) and proteins specialized to initiate signaling cascades by localizing partners at the cell membrane (docking proteins) This...

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Báo cáo khoa học: Compartmentalized signalling: Ras proteins and signalling nanoclusters pdf

Ngày tải lên : 07/03/2014, 00:20

... signalling J Omerovic and I A Prior Fig Ras activation and effectors Ras is a molecular switch cycling between inactive GDP- and active GTP-bound conformations controlled by GEFs and GAPs Active Ras ... now switching towards understanding context-dependent regulation of interactions and outputs within this large signalling web Ras trafﬁcking and localization H-, K-, and N-Ras share almost complete ... Ras GEFs, GAPs and scaffolds Ras has been localized to and signals from both cell surface and endomembrane platforms Ras regulators are also differentially localized, many of the proteins illustrated...

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Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc

Ngày tải lên : 07/03/2014, 12:20

... interconversion between Ala and Glu, Glu and Asp, and Glu and Gln is efﬁcient in wheat germ extract but can effectively be suppressed by inhibitors of transaminases and glutamine synthase [20] ... scrambling and easier sample handling compared to in vivo protein production render cell-free protein synthesis particularly attractive for high-throughput production of proteins and selective ... amino acids and the necessity to purify each individual sample In contrast, cell-free systems allow the synthesis of [15N]-labelled proteins with very small quantities of [15N]-amino acids and they...

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Báo cáo khoa học: Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-association domains pptx

Ngày tải lên : 07/03/2014, 12:20

... 1122–1304, 1305–1398, 1305–1462, and 1399–1462) fusion proteins or with GST alone; bound proteins were eluted, separated by SDS ⁄ PAGE, and visualized by autoradiography (C) The proteins for the GRIP1 ... interacting proteins, CoCoA and GAC63 [29,30] In this study, we investigated the regulation of GRIP1 transactivation and co-activation activities by its own C terminus through the repression and selfassociation ... the modulation of self- transactivation (AD1 and AD2) and co-activator (AR, ER and TR) functions in HeLa cells The outcome of the relationship between GRIP1 transactivation and co-activator functions...

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Báo cáo khoa học: Computer-assisted mass spectrometric analysis of naturally occurring and artiﬁcially introduced cross-links in proteins and protein complexes potx

Ngày tải lên : 07/03/2014, 12:20

... disulfide bonds, and artificially introduced cross-links in proteins Cross-links impose distance constraints on amino acid residues that can be used to model the 3-D structure of proteins and protein ... disulfide bond structures, chemical modifications and cross-linking in protein mixtures, complexes, and assemblies Proteins A single protein, a list of proteins from a mixture or from a protein complex ... for searching unmodified and post-translationally modified peptides, peptides with an internal disulfide or chemically induced cross-link (intrapeptide cross-link products), and peptide pairs bonded...

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Báo cáo khoa học: Comprehensive sequence analysis of horseshoe crab cuticular proteins and their involvement in transglutaminase-dependent cross-linking potx

Ngày tải lên : 07/03/2014, 21:20

... through Y9), and repeats of the di- and tri-peptide sequences QQ and QQQ were observed in the amino terminal sequences of basic QH proteins and 10 Nucleotide sequences of HMM chitin-binding proteins ... chitin-binding proteins Basic G13 and G19, acidic DE25 and DE29 and S37 were shown by RT-PCR to be expressed predominantly in epidermis (Fig 7) In contrast, basic Y6, Y7 and QH4 were broadly expressed, and ... G13 variants, G19 and G19 h all contained an R & R consensus sequence, and they exhibited signiﬁcant sequence similarity (G19 and G13, 58% identity; G19 h and G13, 48%; G19 and G19 h, 64%) A...

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Báo cáo khoa học: Dynamin-related proteins and Pex11 proteins in peroxisome division and proliferation doc

Ngày tải lên : 07/03/2014, 21:20

... domain, and the C-terminal GED (Fig 2) Obvious PRDs or PH domains are not found in DRPs Examples of DRPs are Mx proteins, mammalian DLP1, and the yeast proteins Dnm1, Mgm1 and Vps1 Mx proteins ... GIM5A and GIM5B These two proteins are nearly identical in sequence and show weak similarity to Pex11 Both are 26 kDa, have two putative 5172 S Thoms and R Erdmann transmembrane domains, and ... peroxisomal matrix and membrane proteins, yet contain vesicular structures with some peroxisomal proteins [61] Pex28 and Pex29 from S cerevisiae are also peroxisomal membrane proteins [62] They...

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Báo cáo khoa học: N-glycosylation inﬂuences the structure and self-association abilities of recombinant nucleolin pot

Ngày tải lên : 14/03/2014, 23:20

... These recombinant glycoproteins were produced and characterized by MALDI-TOF and GC ⁄ MS, CD and fluorimetry, and their self- interaction properties were investigated by PAGE and surface plasmon resonance ... recombinant proteins, and the influence of N-glycosylation site occupancy in the structure of the proteins SPR and PAGE studies confirm the ability of the nucleolinderived proteins to self- interact, and ... determine the glycosylated peptides present in recombinant proteins, NCT and RCT proteins were submitted to reduction alkylation and digested by trypsin Glycopeptides were isolated and analyzed by MS...

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Báo cáo khoa học: Phosphorylation modulates the local conformation and self-aggregation ability of a peptide from the fourth tau microtubule-binding repeat pdf

Ngày tải lên : 16/03/2014, 05:20

... peptide R4, both CD and NMR spectroscopy were performed In NMR spectroscopy, TOCSY and NOESY spectra of the two peptides were recorded and compared Changes in the backbone NH and aH chemical shifts ... shifts of NH and aH between the nonphosphorylated and phosphorylated peptides is summarized in Fig The chemical shift deviations of NH and aH reﬂect changes in the electrostatic state and molecular ... differences between phosphopeptide and nonphosphopeptide were analysed using CD and highresolution NMR spectroscopy The aggregation behavior of peptide R4 and phosphopeptide pR4 and the structural differences...

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Báo cáo khoa học: A novel mechanism of TGFb-induced actin reorganization mediated by Smad proteins and Rho GTPases docx

Ngày tải lên : 16/03/2014, 06:20

... also enhanced by overexpression of Smad3 and Smad4 proteins (3.4-fold) and to a lesser extent by overexpression of Smad2 and Smad4 proteins (2.3-fold), and this activity was stimulated further ... promoter Smad2 and Smad3 proteins activate RhoA and RhoB GTPases and induce actin polymerization and microfilament reorganization in Swiss 3T3 fibroblasts To examine whether Smad proteins, in addition ... min, and GST–RBD was used to isolate GTP-loaded RhoA and RhoB from cell lysates Both proteins were monitored by immunoblotting using anti-RhoA and antiRhoB specific serum, and the protein band...

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Báo cáo khoa học: Separation and characterization of caveolae subclasses in the plasma membrane of primary adipocytes; segregation of speciﬁc proteins and functions docx

Ngày tải lên : 16/03/2014, 13:20

... of protein (lanes and 4, VHD-caveolae; lanes and 5, HD-caveolae; lanes and 6, LD-caveolae) The amount of the analysed proteins is expressed as arbitrary densitometric units and normalized to ... (lanes b1 and b2); LD-caveolae (lane c) a, b1 and b2, and c were separately subjected to SDS ⁄ PAGE (9% acrylamide) and silver staining Indicated are apparent molecular masses of reference proteins ... the functional and dynamic relationships between closed Identiﬁcation of caveolae subclasses and open caveolae, as well as how targeting and sequestration of the different proteins and lipids are...

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