β induced increase in level of nitric oxide in bv2 cells
báo cáo hóa học: " Sinomenine inhibits microglial activation by Ab and confers neuroprotection" doc
... nitric oxide in BV2 cells Ab is known to increase levels of inducible nitric oxide synthase (iNOS) in microglial cells [25,26] Since the induction of iNOS is associated with increased production of ... of BV2 cells with ADDL led to a significant increase in the level of NO However, sinomenine reduced NO level (Figure 2) Thus, sinomenine inhibits ADDL -induced enhancement of NO level in BV2 cells ... concentrations of sinomenine before addition of ADDL ADDL significantly increased the level of ROS in BV2 cells However, pretreatment with sinomenine inhibited ADDL -induced increase in ROS level (n = in...
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Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx
... mode of actions of TS, we examined the effect of TS on LPS/IFNinduced NO production in VSMC We found that TS activated LPS/IFN -induced NO production in VSMC through enhancement of iNOS protein ... induce apoptosis in various cell lines [8–15,29], the inhibitory effect of TS on the cell growth at higher concentrations might prevent the increase in the enhancing effect of TS on LPS/IFN -induced ... to the cells, and then the cell suspension was sonicated in a bath-type sonicator for 5min The amount of protein of the solubilized cells was determined with a bicinchoninic acid protein assay...
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Báo cáo khoa học: Modulation of nitric oxide-mediated metal release from metallothionein by the redox state of glutathione in vitro doc
... combination of tighter binding of cadmium than zinc in metallothioneins and the preference of zinc in the more flexible b-domain In addition to the already described differences in flexibility of ... suppression of the expression and activity of inducible nitric oxide synthase (iNOS) by zinc [32,33], released from MT under the in uence of nitric oxide (NO), and the scavenging of NO through ... cells from the cytotoxic and DNA-damaging effects of nitric oxide [29] So, while the binding of NO to GSH in vivo does not obviously prevent NO from interacting with MT2, we have shown that in...
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Báo cáo khoa học: A pathway through interferon-c is the main pathway for induction of nitric oxide upon stimulation with bacterial lipopolysaccharide in mouse peritoneal cells pot
... by anti-IFN-c Ig, of LPS -induced NO production in murine PEC but not in the RAW264.7 murine macrophage cell line Cells of murine PEC and of the RAW264.7 murine macrophage cell line were cultured ... production of NO in macrophages stimulated with LPS, via activation of NF-jB and its binding to region in the promoter of the iNOS gene [11,12] In the present study, LPS -induced NO production in the ... to clarify the role of IFN-c in LPS -induced NO production and revealed the important role of IFN-c as a key mediator in the main pathway for LPS -induced NO production in murine peritoneal cell...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
... previously to induce an increase in the level of [Ca2+]i and a correlated calpain activation [28] This increase in [Ca2+]i is more intense in aorta than in brain Under these conditions, in brain, no ... cleavage of inducible nitric oxide synthase (iNOS) by calpain I Biochim Biophys Acta 1568, 216– 224 23 Su Y & Block ER (2000) Role of calpain in hypoxic inhibition of nitric oxide synthase activity in ... right amount of nitric oxide Am J Physiol Renal Physiol 285, 178–190 In vivo degradation of NOS and HSP90 by calpain Kone BC (2000) Protein–protein interactions controlling nitric oxide synthases...
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báo cáo hóa học: " Signaling pathways mediating a selective induction of nitric oxide synthase II by tumor necrosis factor alpha in nerve growth factor-responsive cells" pptx
... induction of iNOS was determined in cells cultured for 24 hr in serum free or in defined medium N2 (Figure 2D) There was a detectable iNOS induction in both serum free- and defined medium-cultured cells, ... TNFα in the presence A: absence blots detecting iNOS in total NGF in A: Western blots detecting iNOS in total protein extracts from PC12 cells treated for 24 hr with increasing concentrations of ... forebrain cholinergic neurons [57] and direct TNFα administration in the brain of mice results in an impairment of basal forebrain cholinergic function [58] However, whether induction of iNOS and...
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Báo cáo khoa học: "Enhanced expression of constitutive and inducible forms of nitric oxide synthase in autoimmune encephalomyelitis" pdf
... the inflammatory cells in the pathogenesis of EAE, as does iNOS in EAE [45] However, our study did not support the finding of EAE in iNOS knockout mice in which both nNOS and eNOS were not increased ... iNOS may be involved in the process of apoptosis [40] In conclusion, our results showed that the three isoforms of NOS including nNOS, eNOS, and iNOS were increased in the initiation of EAE and ... expression of both nNOS and eNOS in the spinal cords of rats with EAE, small amounts of iNOS were identified in the normal spinal cords but its expression slightly increased in the spinal cord of 5CFAtreated...
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Báo cáo khoa học: "Expression of nitric oxide synthase isoforms in the porcine ovary during follicular development" ppsx
... and inducible (iNOS) isoforms of nitric oxide synthase (NOS) in the ovaries of pigs The intensity of staining is indicated by (-), where staining was absent, up to (+++), for maximal staining ... immunostaining was detected in granulosa or theca cells In the later stages, including Graafian follicles, immunostaining for three isoforms of NOS was detected in the granulosa and theca cells ... expression of iNOS in porcine ovaries is well known, our findings contrast in part with the previous report [12] In the present study, iNOS was mainly localized to the oocytes of unilaminar and...
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Báo cáo y học: "Protective effects of total fraction of avocado/soybean unsaponifiables on the structural changes in experimental dog osteoarthritis: inhibition of nitric oxide synthase and matrix metalloproteinase-13" docx
... Nitric oxide mediates interleukin-1 induced inhibition of glycosaminoglycan synthesis in rat articular cartilage Mediators Inflamm 1995, 4:107-111 48 Murrell GAC, Jang D, Williams RJ: Nitric oxide ... reduce MMP-13 mRNA in murine chondrocytes in monolayer culture under stimulation with IL 1β [28] MMP-13 expression is increased in tissue that is in need of repair or remodelling, as in OA MMP-13 was ... and incubated in the presence of the biotin-conjugated secondary antibody for 45 minutes at room temperature followed by the addition of the avidin-biotin-peroxidase complex for 45 minutes All incubations...
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Báo cáo y học: "Suppression of nitric oxide production from nasal fibroblasts by metabolized clarithromycin in vitro" potx
... decrease in iNOS expression in cytosol after inflammatory stimulation, inhibiting superoxide generation and resulting in prevention of tissue injury in patients with chronic airway diseases, including ... LPS -induced iNOS expression in murine macrophages and hepatocytes [36,37] The protein kinase C (PKC) pathway is also reported to be involved in LPS -induced iNOS expression, and activation of PKC isoforms, ... bleomycin -induced pulmonary fibrosis in mice by the inhibition of TGF-beta signaling in lung fibroblasts Respir Res 2006, 7:16-29 13 Tayler BS, Geller DA: Regulation of the inducible nitric oxide...
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Báo cáo y học: "Suppression of nitric oxide production from nasal fibroblasts by metabolized clarithromycin in vitro" pptx
... decrease in iNOS expression in cytosol after inflammatory stimulation, inhibiting superoxide generation and resulting in prevention of tissue injury in patients with chronic airway diseases, including ... LPS -induced iNOS expression in murine macrophages and hepatocytes [36,37] The protein kinase C (PKC) pathway is also reported to be involved in LPS -induced iNOS expression, and activation of PKC isoforms, ... bleomycin -induced pulmonary fibrosis in mice by the inhibition of TGF-beta signaling in lung fibroblasts Respir Res 2006, 7:16-29 13 Tayler BS, Geller DA: Regulation of the inducible nitric oxide...
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Báo cáo y học: " Central role of nitric oxide in the pathogenesis of rheumatoid arthritis and systemic lupus erythematosus" potx
... for increased endogenous NO synthesis, suggesting that overproduction of NO may be important in the pathogenesis of RA The in amed joint in RA is the predominant source of NO [45,46] Several investigators ... that decreased TCRζ chain expression may also be independent of NO in SLE [40-44] Consequences of increased nitric oxide production in rheumatoid arthritis Several studies in patients with RA have ... ζ chain in SLE T cells [64], which may explain the enhanced T-cell-activation -induced Ca2+ fluxing The potential role of NO in the regulation of FcR gamma expression clearly needs further investigation...
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Báo cáo y học: "Involvement of nitric oxide (NO) in cough reflex sensitivity between non-sensitized and OVA-sensitized guinea pigs" pptx
... experiment using the selective iNOS inhibitor ONO1714, which lowered ENO levels in both groups ONO1714 reduced the OVAinduced increase in cough reflex sensitivity only in sensitized guinea pigs, ... performed the iNOS inhibition experiment using NOS inhibitors, in non-sensitized guinea pigs with normal cough reflex sensitivity and in OVA-sensitized guinea pigs which were of increased cough ... [29] These findings indicate that cNOS inhibition seems to be detrimental, and iNOS inhibition is a possible target of novel therapeutic agents in bronchial asthma Disappointingly, Singh [30] reported...
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Báo cáo y học: "Time course of nitric oxide synthases, nitrosative stress, and poly(ADP ribosylation) in an ovine sepsis model" pot
... Cauwels A: Nitric oxide in shock Kidney Int 2007, 72:557-565 24 Su CF, Yang FL, Chen HI: Inhibition of inducible nitric oxide synthase attenuates acute endotoxin -induced lung injury in rats Clin Exp ... expressed by circulating cells, such as neutrophils [28,29] Consequently, an injury-related increase in inflammatory cells may account for increased protein expression of constitutive NOS in the current ... protein expression of nNOS was increased at any of the evaluated time points, both eNOS and iNOS protein expressions started to increase early after the injury Albeit the changes in mRNA of eNOS...
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The role of nitric oxide and other gaseous mediators in cardiovascular disease models; emphasis on septic shock
... 2: Biology of Nitric Oxide (NO) 2.1 Biosynthesis In mammalian cells, nitric oxide (NO) arises from the enzymatic oxidation of a terminal guanidino nitrogen of the amino acid, L-arginine (Furchgott ... puncture (CLP) -induced septic shock model Induction of sepsis is intrinsically more challenging than inducing endotoxemia since the immune challenge is episodic in nature Some of the guidelines for ... NO Nitric oxide NOF (HCT-1026) Nitroflurbiprofen NOS Nitric oxide synthase nNOS (NOS I) Neuronal nitric oxide synthase NOx Nitrate/nitrite NO-NSAID Nitric oxide- releasing non-steroidal anti-inflammatory...
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The role of nitric oxide and prostaglandin e2 in prolonged hemorrhagic shock 2
... role nitric oxide plays in a combine rat model of prolonged hemorrhagic shock and fluid percussion injury (induction of traumatic brain injury) Nitric oxide (NO) has been implicated as being an ... was no change in creatinine levels in sham-operated animals (261.66 75.14 mol/L) when compared with normal rats However, a marked increase in plasma creatinine level was found in salinetreated prolong ... in maintaining mean arterial blood pressure (MABP) and increasing the survivability of the shocked animals NOS inhibitors have been shown to be able to maintain a high MABP, after shock was induced, ...
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The role of nitric oxide and prostaglandin e2 in prolonged hemorrhagic shock 1
... Shirhan Md Mercaptoethylguandine inhibition of inducible nitric oxide synthase and cyclooxygenase-2 expressions induced in rats following fluid-percussion brain injury Journal of Trauma 59(2), 2005 ... levels of rat brain in different groups of rats 37 Figure 3.5 GOT levels of rat brain in different groups of rats 38 Figure 3.6 Nitrate/nitrite content of rat brain in different groups of rats ... and eosin stain of Kidneys 62 Figure 4.4.2 Hemotoxylin and eosin stain of Lungs 63 Figure 4.4.3 Hemotoxylin and eosin stain of Livers 64 Figure 4.4.4 Hemotoxylin and eosin stain of Cerebral cortex...
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Role of nitric oxide in wound healing facilitatory effects of nitrosoglutathione a nitric oxide donor on the extracellular matrix deposition characteristics of wound healing
... healing 57 1.5.3.1 Nitric Oxide donors previously studied in wound healing 1.5.3.2 Nitric Oxide inhibitors previously studied in wound healing 59 1.5.3.3 Nitrosothiols 60 1.5.3.4 Interaction of Nitric ... expression of Nitric oxide synthase in normal human skin and Keloid: Effect of Steroids on the expression of NO in keloids In: Wound Healing Society Conference, Institute of health, Singapore, ... healing- Role Of nitric oxide in the reparative process In: Wound Healing Society Conference, Institute of health, Singapore, Oct 1999 4) Achuth HN, SM Moochhala, WTL Tan Effect of nitric oxide...
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... no increase in the phosphorylated JNKs protein level in the cell line Twenty-four-hour exposure to hypoxia resulted in a Int J Med Sci 2007, 42 marked increase (1.8-fold of control) in the level ... other hand, the protein expression of caspase 3, PTEN, and JNK1 in JAR cells exhibited a 4.96-fold increase, 1.92-fold increase, and in the level of phosphorylated JNK 1&2 for JAR cells with Bag-1 ... of protein changes for the experimental sample to control detected by using the Odyssey Infrared Imaging System (+) means an increase of signal intensity, and (-) means a decrease of signal intensity...
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Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... factor (ATF4) and of CCAAT ⁄ enhancer-binding protein homologous protein (CHOP) proteins downstream of eIF2a were observed (Fig 4A), indicating the initiation of ER stress -induced apoptosis Oleate ... mitochondrial fraction following stearate administration (Fig 3B) The presence of oleate inhibited this translocation, indicating the involvement of Bax activation in stearate -induced mitochondrial ... proposed to be involved in SFA -induced lipotoxicity [8,13,16,23] As shown in Fig 4A, increased phosphorylation of eukaryotic translation initiation factor 2a (eIF2a) was Saturated fatty acid-induced...
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