α5 in pulmonary epithelium by foxa2 and gata 6
Báo cáo y học: "Hypoxia-inducible factor-1 a/platelet derived growth factor axis in HIV-associated pulmonary vascular remodeling" pps
Ngày tải lên :
12/08/2014, 13:22
... hypoxia in mice partially deficient for hypoxia-inducible factor 1alpha J Clin Invest 1999, 103(5) :69 1 -69 6 54 Rosenberg HC, Rabinovitch M: Endothelial injury and vascular reactivity in monocrotaline ... result of integrated effects of various HIV proteins and the related inflammatory mediators including PDGF-BB Examination of the HIV-1 Tg rat lungs revealed increased staining of PDGF-BB in macrophages ... Simonneau G, Emilie D: Increased interleukin-1 and interleukin -6 serum concentrations in severe primary pulmonary hypertension Am J Respir Crit Care Med 1995, 151(5): 162 8- 163 1 Humbert M, Monti...
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Tài liệu Báo cáo khoa học: Myristoylation of the dual-specificity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf
Ngày tải lên :
16/02/2014, 14:20
... resuspended in 2· Laemmli sample buffer, and proteins were resolved by SDS ⁄ PAGE The gel was fixed in 40% methanol ⁄ 10% acetic acid, and stained with SYPRO Ruby Protein Gel Stain (Invitrogen) according ... PTP domain b-CH3SOH b ions y ions y-CH3SOH 522.33 5 86. 33 OxM 408.19 344.19 268 .23 382.27 439.29 Myr-Gly Asn Gly 579. 26 465 .21 515. 26 401.21 MH+ MH+- 8 46. 48 63 6.37 700.37 Asn Lys 261 . 16 147.11 ... pathway, binding of death ligands to their respective receptors recruit adaptor proteins, such as Fas-associated death domain protein, which in turn bind and aggregate FEBS Journal 277 (2010) 2 463 –2473...
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Báo cáo y học: "Vpu serine 52 dependent counteraction of tetherin is required for HIV-1 replication in macrophages, but not in ex vivo human lymphoid tissue" docx
Ngày tải lên :
12/08/2014, 23:22
... in Vpu is involved in the down-modulation and the degradation of tetherin (Fig 1, 6) [7,9-11,19] While most groups investigated Vpu with mutations in both serines at positions 52 and 56 (S2 /6) , ... of tetherin and CD4 in primary T-cells and macrophages by Vpu (A) FACS analysis of CD4 and tetherin modulation in infected PBL cultures PBL were infected with HIV-1 variants expressing eGFP via ... [25,50] P4-CCR5 and 293T cells were maintained in Dulbecco’s modified Eagle’s medium containing 10% heat-inactivated fetal bovine serum The human Jurkat T-cell line was cultured in RPMI 164 0 medium...
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Báo cáo y học: "The HIV-1 Rev/RRE system is required for HIV-1 5’ UTR cis elements to augment encapsidation of heterologous RNA into HIV-1 viral particle" ppsx
Ngày tải lên :
13/08/2014, 01:21
... RNA in viral particles, and in producer cells Surprisingly, as shown in Figure A and 3C, in contrast to the increase in vector titers (Figure 1B), incorporation of the 5’ UTR cis elements into ... S, Rein A: Selective and nonselective packaging of cellular RNAs in retrovirus particles J Virol 2007, 81 :66 23 -66 31 Ikeda Y, Takeuchi Y, Martin F, Cosset FL, Mitrophanous K, Collins M: Continuous ... fragment, consisting of the canonical packaging signal and extending into the 5’ end of Gag and HIV-1 RRE, into the BamHI sites of pTK1332 Upon cutting with BamHI the existing RRE in pTK1332 would...
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Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx
Ngày tải lên :
16/03/2014, 04:20
... chimeric proteins to investigate the role of intramolecular interactions in determining the kinetics of carnitine palmitoyltransferase I isoforms J Biol Chem 275, 19 560 –19 566 16 Nicot C, Relat ... glutamate-3 valine-19 leucine-23 and serine-24 with alanine in the N-terminal region of human heart muscle carnitine palmitoyltransferase I abolishes malonyl CoA inhibition and binding Arch Biochem ... cDNA The inserts (548 and 518 bp, respectively) were liberated and ligated in the digested ApaI and HindIII PMCPT1STOP–BSSK+ (ApaI is also included in the BSSK+ polylinker), resulting in constructs...
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Báo cáo khoa học: Passage through the Golgi is necessary for Shiga toxin B subunit to reach the endoplasmic reticulum pptx
Ngày tải lên :
16/03/2014, 04:20
... followed by protein toxins into cells Int J Med Microbiol 293, 483–490 Johannes L & Decaudin D (2005) Protein toxins: intracellular trafficking for targeted therapy Gene Ther 12, 1 360 –1 368 Mallard ... Cell 13, 262 –275 Khine AA, Tam P, Nutikka A & Lingwood CA (2004) Brefeldin A and filipin distinguish two globotriaosyl ceramide ⁄ verotoxin-1 intracellular trafficking pathways involved in Vero cell ... retrotranslocate into the cytosol by exploiting the translocon [ 16, 66] In this case, the current retrograde transport of StxB and of holotoxin may have developed as a series of host–pathogen interactions and...
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Báo cáo Y học: Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene ppt
Ngày tải lên :
17/03/2014, 23:20
... Chem 261 , 10375–10379 51 Lees-Miller, S.P., Chen, Y.R & Anderson, C.W (1990) Human cells contain a DNA-activated protein kinase that phosphorylates 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 simian ... Ku protein to bind DNA in a sequence-independent manner [48,49] In the MREa–protein complex detected by EMSA (Fig 2A), both the 82- and 70-kDa proteins were visualized by silver staining (unpublished ... proteins were incubated with a biotinylated MREa oligonucleotide and trapped by avidin– agarose beads The proteins were extracted from the avidin– agarose beads by washing with a buffer containing...
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Báo cáo khoa học: Activation of p21-activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells potx
Ngày tải lên :
30/03/2014, 13:20
... PAK1 activation by LPA was efficiently inhibited by tyrosine kinase inhibitor, genistein, but not by ROCK inhibitor, Y2 763 2 (Fig 2A), indicating that RhoA might not be involved in LPA-induced PAK1 ... other proteins, including paxillin, p130Cas, vinculin and talin in focal adhesions These protein complexes anchor the actin cytoskeleton and provide structural integrity to cells [ 16] The importance ... PAK in A2058 cells, cells were pretreated with either tyrosine kinase inhibitor, genistein, or MAP kinase inhibitor, PD98059 Inhibition of tyrosine kinase by genistein greatly inhibited LPA-induced...
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báo cáo khoa học: " Sgt1, but not Rar1, is essential for the RB-mediated broad-spectrum resistance to potato late blight" pptx
Ngày tải lên :
12/08/2014, 05:20
... controlled by single dominant resistance genes (R genes) These R genes are only effective in preventing the development of late blight if the invading P infestans race contains the corresponding avirulence ... cysteine- and histidine-rich (CHORD) Zn2+-binding domains and was proposed to play a role in stabilizing R proteins in a confirmation that is implicated in receiving pathogen signals [9] The Sgt1 gene ... loading of mRNA in each lane in determining resistance scores The average score of the late blight infection on the Rar1-silenced plants was 7.3 (± 0 .6) after days post inoculation (dpi) and 6. 9...
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Báo cáo y học: " PDZ domain-binding motif of human T-cell leukemia virus type 1 Tax oncoprotein is essential for the interleukin 2 independent growth induction of a T-cell line" ppsx
Ngày tải lên :
13/08/2014, 09:21
... same extent as Tax1, and interacts with Dlg and MAGI-3 (Table 1) [33, 36] Thus, the binding of Tax2B+C to PDZ domain-containing proteins is not sufficient to induce IL-2-independent growth Although ... genes in HTLV-I infected T-cell lines: possible involvement of Tax1 in the altered expression of cyclin D2, p18Ink4 and p21Waf1/Cip1/Sdi1 Oncogene 19 96, 12: 164 5- 165 2 Mori N, Fujii M, Hinz M, ... experiments using their chimeric proteins indicated that the PDZ domain-binding motif (PBM) located at the Tax1 C-terminus, S/TXV (S/T, serine or threonine; X, any amino acid; V, valine), is responsible...
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MECKELIN 3 IS NECESSARY FOR PHOTORECEPTOR OUTER SEGMENT DEVELOPMENT
Ngày tải lên :
24/08/2014, 11:10
... function in kidney, liver, and retina [58] Meckelin and Related Proteins Meckelin, the MKS3 gene protein product, comprises of 995 amino acids in human and mouse and 997 amino acids in rat Previous ... stained with Harris hematoxylin solution (1x) for minutes Next, wash slides in running tap water for 5-10 16 minutes Sections were then stained with eosin Y solution for minute and dipped in ... development in the WT and WPK littermates Sections through the central retina of WT and mutant were stained with hematoxylin and eosin and the thickness of the outer (ONL), inner (INL), and ganglion...
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Tài liệu Báo cáo khoa học: Mitogen-activated protein kinase phosphatase-1 modulated JNK activation is critical for apoptosis induced by inhibitor of epidermal growth factor receptor-tyrosine kinase pdf
Ngày tải lên :
18/02/2014, 13:20
... was initiated by the addition of kinase buffer containing 100 lm ATP, samples were incubated at 30 °C for 45 min, and reactions were terminated by the addition of SDS sample buffer and heating ... apoptosis in PC-9 cells Stress stimuli that induce apoptosis, including UV- and c-irradiation, heat shock, protein synthesis inhibitors, DNA-damaging agents and the proinflammatory cytokines, are ... their survival and, consequently, undergo apoptosis following inhibition of EGFR tyrosine kinase by gefitinib Gefitinib has been shown to inhibit cell survival and growth signaling pathways such...
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Báo cáo khoa học: The calcium-binding domain of the stress protein SEP53 is required for survival in response to deoxycholic acid-mediated injury pdf
Ngày tải lên :
23/03/2014, 10:21
... cells were split and plated in media containing Geneticin to select for cell containing vector DNA After three weeks, the number of cells was determined by fixing cells and staining with dye: vector ... in media containing Geneticin to select for cell containing vector DNA (as in Fig 5A,G) After three weeks, the number of cells was determined by fixing cells and staining with dye Individual colonies ... proteins was within the first 90 amino acids, which contain the two helix–turn–helix sequences of the EF-hand calcium-binding motifs The calcium-binding FEBS Journal 273 (20 06) 1930–1947 ª 20 06 The...
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Báo cáo hóa học: " Evidence that the Nijmegen breakage syndrome protein, an early sensor of double-strand DNA breaks (DSB), is involved in HIV-1 post-integration repair by recruiting the ataxia telangiectasia-mutated kinase in a " pptx
Ngày tải lên :
20/06/2014, 01:20
... retroviral DNA integration Science 1999, 284 :64 4 -64 7 Downs JA, Jackson SP: Involvement of DNA end-binding protein Ku in Ty element retrotransposition Mol Cell Biol 1999, 19 :62 60 -62 68 Lau A, Swinbank ... reported to induce G2 cell-cycle arrest by triggering the ATR-dependent checkpoint cascade [38] Hypothetically, vpr could increase HIV-1 transduction by inducing the growth arrest, thereby giving the ... (GM07 166 ) and matched controls (GM045 06) were infected with HIV-1-based vector carrying the lacZ reporter at an m.o.i of 0.25 Five and seven days post infection (dpi) cells were stained using a...
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Báo cáo y học: " Viral replication is enhanced by an HIV-1 intersubtype recombination-derived Vpu protein" pot
Ngày tải lên :
12/08/2014, 01:22
... occurring recombinants A serine residue, either at position 61 or 64 (S61 or S64) has been shown to be involved in the regulation of Page of Vpu degradation process [ 16] Neither S61 nor S64 were ... breakpoint is located in the region coding for an amino acid stretch predicted to form a random-coil structure [20] This region contains two serine residues (S52 and S 56) , targeted by the casein kinase ... used to infect CEM-GFP cell culture, in duplicate, and maintained up to 21 days post-infection (p.i.) Viral population analysis was performed on days 1, 12 and 21 p.i by cloning and sequencing the...
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Báo cáo y học: " The formation of cysteine-linked dimers of BST-2/tetherin is important for inhibition of HIV-1 virus release but not for sensitivity to Vpu" potx
Ngày tải lên :
12/08/2014, 23:22
... either individually into BST-2 to result in mutants N1 (N65Q) and N2 (N92Q), respectively, or in combination to result in mutant N1/N2 (N65,92Q) Mutants were expressed in 293T cells and analyzed by ... residues in its extracellular domain that could be involved in the formation of cysteine-linked dimers (Fig 1) We mutated the cysteines at positions 53, 63 , and 91 individually or in combinations ... asparagine residues N65 and N92 to glutamine in human BST-2 either alone or in combination PCR products were cloned into pcDNA-BST-2 to obtain pcDNA-BST-2 N1 (N65Q), pcDNA-BST-2 N2 (N92Q), and pcDNA-BST-2...
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Báo cáo y học: "b-TrCP is dispensable for Vpu’s ability to overcome the CD317/Tetherin-imposed restriction to HIV-1 release" pot
Ngày tải lên :
13/08/2014, 01:20
... hydrophilic C-terminal domain of Vpu [ 26, 33], Vpu is phosphorylated at serine residues 52/ 56 by casein kinase II [33,34], allowing for recruitment of an E3 ubiquitin ligase multi-protein complex via ... Block TM, Guo JT: Interferoninduced cell membrane proteins, IFITM3 and tetherin, inhibit vesicular stomatitis virus infection via distinct mechanisms J Virol 2010, 84(24):1 264 6-1 265 7 Van Damme N, ... Cells and plasmids 293T, 293TCD4, 293HA-CD317, TZM-bl, and HeLaP4 cells were maintained in DMEM supplemented with 10% fetal calf serum, 1% penicillin-streptomycin and 1% L-glutamine (all from Invitrogen)...
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Báo cáo y học: "Matrin 3 is a co-factor for HIV-1 Rev in regulating post-transcriptional viral gene expressio" pptx
Ngày tải lên :
13/08/2014, 01:21
... integrin binding CIB1; CIB; kinase-interacting protein 1; KIP1 NP_0 063 75 Cleavage stimulation factor, 3’ pre-RNA, subunit 2, 64 kD, tau CSTF2T; KIAA 068 9 NP_0 560 50 Homeodomain-interacting protein kinase ... ARNT, hypoxia-inducible factor 1, beta subunit; dioxin receptor NP_00 165 9 Calcium and integrin binding CIB1; CIB; KINASE-INTERACTING PROTEIN 1; KIP1 NP_0 063 75 DAZ associated protein DAZAP2 NP_055579 ... interacting proteins identified by yeast hybrid assay PTB-1 interacting proteins identified by yeast hybrid assay Other names/synonyms Accession # Aladin AAAS; adracalin NP_0 564 80 Calcium and integrin...
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Báo cáo y học: "Common principles and intermediates of viral protein-mediated fusion: the HIV-1 paradigm" pps
Ngày tải lên :
13/08/2014, 05:21
... signaling and actin remodeling to enhance infection [ 163 , 164 ] For instance, HIV Env-mediated signaling via CD4 and/ or coreceptors has been implicated in productive entry [18,39,50, 165 -170] and ... http://www.retrovirology.com/content/5/1/111 166 167 168 169 170 171 172 173 174 175 1 76 177 178 179 Martínez-A C, Mañes S: Statins inhibit HIV-1 infection by downregulating Rho activity J Exp Med 2004, 200:541-547 Lin YL, Mettling C, ... (residues 598 60 4) and cluster II (residues 64 4 66 3) overlapping the gp41 loop and HR2 domain, respectively, are transiently exposed during fusion [1 16- 118] The HR1, HR2 and loop domains become...
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Báo cáo y học: " MDM2 is a novel E3 ligase for HIV-1 Vif" pot
Ngày tải lên :
13/08/2014, 05:21
... that may be involved in the ubiquitination of Vif During a search for Vif-interacting proteins in the HIV, Human Protein Interaction Database of National Institute for Allergy & Infectious Diseases ... MDM2 mutants binding to Vif are shown determine a Vif-binding domain, we further tested mutants deleted in a Zn Finger domain (ΔZn) or in an acidic domain (ΔAD) Neither mutant could bind Vif, whereas ... ubiquitination and degradation [20]), Cul5 (another RING finger type E3 that forms a complex with Vif and is reported to induce Vif ubiquitination [17,21]), and Parkin (another RING finger type...
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