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Plastid Biology, Advances in Plant Biology Volume 5

Ngày tải lên : 11/06/2015, 11:24

... space, or in response to environmental clues [25, 106, 131, 132, 187, 237] Notably, however, such quantitative editing changes are likely to be superseded by much larger variations in the abundances ... Drechsel O, Bock R (2007) OrganellarGenomeDRAW (OGDRAW): a tool for the easy generation of high-quality custom graphical maps of plastid and mitochondrial genomes Curr Genet 52:267–274 167 Loiselay...

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Báo cáo sinh học: "Respiratory syncytial virus infection in Fischer 344 rats is attenuated by short interfering RNA against the RSV-NS1 gene" doc

Ngày tải lên : 14/08/2014, 19:22

... a concentration of 0.02% and heated at 55°C for 10 The plasmid solution was then mixed with an equal amount of chitosan solution (5:1, weight to weight ratio of chitosan to DNA) and vortexed at ... group) were given a single intranasal dose of 200 µg of siNS1 or control plasmid complexed with an equal amount of chitosan After 24 h, they were intranasally inoculated with 1.4 × 107 PFU of RSV ... homogenized at 4°C using a Polytron (Brinkmann, Westbury, NY) Lung homogenates were centrifuged and equal amounts (based on the weight of lung homogenized) of supernatants were diluted 1:4 into EMEM...

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Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf

Ngày tải lên : 15/02/2014, 01:20

... fraction was treated overnight with enterokinase at unitsÆ100 lL)1 Quantification of TMPRSS13 The enterokinase-treated pro-TMPRSS13 was quantified by immunoblotting using the protein expressed in E coli ... (C), and analysed by immunoblotting with the anti-c-Myc IgG The protease domain of TMPRSS13 was quantified by scanning densitometry of the immunoblot (B, lane 2) with NIH IMAGEJ software using ... The gel was stained with Coomassie Brilliant Blue (B) The intensity of the band of pro-HGF was quantified with NIH IMAGEJ software and the percentage of HGF processed was calculated (C) Pro-HGF...

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Báo cáo khoa học: Double-stranded RNA-dependent protein kinase (PKR) is downregulated by phorbol ester ppt

Ngày tải lên : 23/03/2014, 13:20

... separated by SDS ⁄ PAGE on 10% gels and subjected to autoradiography (B) PKR autophosphorylation was quantified by PhosphorImager analysis The results represent data from three separate experiments ... for 60 PKR autophosphorylation was determined as described above (B) PKR autophosphorylation was quantified by PhosphorImager analysis The results represent data from three separate experiments ... for 60 PKR autophosphorylation was analysed as described above (B) PKR autophosphorylation was quantified by a densitometry lane 5) or without (lane vs lane4) poly I:C is almost same (Fig 4) There...

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Báo cáo y học: "Collagen type II (CII)-specific antibodies induce arthritis in the absence of T or B cells but the arthritis progression is enhanced by CII-reactive T cells" ppsx

Ngày tải lên : 09/08/2014, 01:24

... transfer of antibodies The cocktail of M2139 and C1 monoclonal antibodies was prepared by mixing equal concentrations of each of the sterile filtered antibody solutions to get a final amount of ... and interferon-γ 101 10–0 10–1 10–2 10–3 10–4 10–5 10–6 As a control, groups of mice received equal volumes of PBS On day 5, lipopolysaccharide (25 µg per mouse) was injected intraperitoneally...

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Báo cáo y học: "Nociceptive tolerance is improved by bradykinin receptor B1 antagonism and joint morphology is protected by both endothelin type A and bradykinin receptor B1 antagonism in a surgical model of osteoarthritis" pdf

Ngày tải lên : 12/08/2014, 15:23

... decay equation: TE S(TE) = M0 exp − T2 where signal intensity S is defined as a function of echo time TE, and is related to the spin density M0 and the transverse relaxation time T The equation ... limb as a percentage of total weight bearing on both hind limbs was calculated by the following equation [37]: % Weight on right leg = Weight on right leg × 100 Weight on right leg + Weight on ... relaxation time T The equation was solved for the mean T2 value over the 16-image stack by using least-squares single-exponential curve-fitting, with initial guesses of M0 = signal intensity at 10 ms...

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KRONE - What is Patch by Exception

Ngày tải lên : 26/10/2013, 19:15

... patented point method of termination which outperforms the competition in ease of installation, quality, and durability of the connection • • • Insulation clamping ribs hold the wire firmly in...

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Tài liệu Báo cáo khoa học: Membrane targeting and pore formation by the type III secretion system translocon pdf

Ngày tải lên : 14/02/2014, 22:20

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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

Ngày tải lên : 18/02/2014, 04:20

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Tài liệu Báo cáo khoa học: High-afﬁnity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

Ngày tải lên : 18/02/2014, 08:20

... Man-6-P ligand binding would require cooperation of both monomers For this purpose, we developed a quantitative assay for heterodimer formation that was based on immunoprecipitation of differentially ... minireceptors were co-precipitated from a cell lysate prepared from cells co-transfected with equal amounts of the tagged mini-receptor cDNA This strongly suggests, but does not prove, that the ... [17] The only difference between Glc-6-P and Man-6-P is the position of the 2-hydroxyl group The equatorial 2-hydroxyl group of Glc-6-P is not in the correct position to make the necessary hydrogen...

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Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efﬁcient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt

Ngày tải lên : 18/02/2014, 12:20

... data were ﬁtted by non-linear regression analysis to the Michaelis–Menten equation v ¼ Vmax Â ½S=ðKm þ ½SÞ or the Hill equation v ¼ Vmax Â ½Sn =ðK0:5n þ ½Sn Þ using prism from GraphPad Software ... G0 and G1 cells to approximately 4–6 lgÆmL)1 (160–240 nm) in peak S-phase cells [12], assuming equal distribution throughout the cytoplasm This indicates that, in G1 ⁄ early S phase, TK1 will ... Nat Struct Biol 8, 616–620 Segura-Pena D, Lichter J, Trani M, Konrad M, Lavie A & Lutz S (2007) Quaternary structure change as a mechanism for the regulation of thymidine kinase 1-like enzymes...

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Tài liệu Báo cáo khoa học: Olfactory receptor signaling is regulated by the post-synaptic density 95, Drosophila discs large, zona-occludens 1 (PDZ) scaffold multi-PDZ domain protein 1 pptx

Ngày tải lên : 18/02/2014, 13:20

... olfactory epithelium of CD1 mice was fractionated by mechanical agitation as described previously [21] Equal amounts of protein from each fraction were loaded on an SDS gel and subjected to immunoblotting...

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Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx

Ngày tải lên : 18/02/2014, 18:20

... nuclear lysate compared to HeLa whole cell lysate Due to the commercial availability of suitable quantities of HeLa nuclear pellets, these were chosen as the starting material for a puriﬁcation ... reduce the level of Y17 phosphorylation (data not shown); however, this result could be due to inadequate knockdown of C-YES or compensation by other Src family members It is known that Src family...

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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc

Ngày tải lên : 19/02/2014, 00:20

... Dynatech, MeLean, VA, USA) 96-well plate reader at a wavelength of 450 nm Cytokine levels were quantiﬁed by comparison with standards The sensitivity of IL-1b and IL-6 was < 0.1 and < 0.7 pgÆmL)1, ... the cells treated with ngÆmL)1 of SEC1 for 24 h The NF-jB-DNA binding activity (lanes 2–8) was quantiﬁed by densitometry The time-course groups were compared with the Ctl group to obtain the ... necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) in the human genome Cold Spring Harb Symp Quant Biol 51 Part 1, 611–624 Spink J, Cohen J & Evans TJ (1995) The cytokine responsive vascular...

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Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf

Ngày tải lên : 19/02/2014, 06:20

... residual molecules in the supernatant (Fig 1C, upper panel) The increasing amounts of extracts quantitatively precipitated the DNA, ending up with saturation at a full extent (lanes and 14) Considering ... and must be an open system Thus, we argue that this organization of nucleoproteins on linear DNA qualiﬁes as coacervation, which has been implicated in the mechanism for the condensed process of ... form of closed circular doublestrand DNA as an undamaged substrate limited the composition and quantity of aggregative proteins (lane 6) It should be noted that proteins that speciﬁcally aggregate...

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Tài liệu Báo cáo khoa học: Selection of stably folded proteins by phage-display with proteolysis docx

Ngày tải lên : 19/02/2014, 12:20

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Tài liệu Báo cáo khoa học: Hu-K4 is a ubiquitously expressed type 2 transmembrane protein associated with the endoplasmic reticulum ppt

Ngày tải lên : 19/02/2014, 17:20

... A 105 75 50 35 + - + + Weak Weak > 54 aa aa T C C T G Weak Weak Adequate Weak Adequate aa 58 aa 13 aa 33 aa 31 aa Adequate Adequate aa 24 aa ATG ATG ATG ATG ATG C B αHu-K4 + PIS Peptide - Peptide ... present, an adequate sequence comprises only one, a strong context both [13] Position ATG1 ATG2 ATG3 ATG4 Kozak consensus A GCC ATG G Context 330 345 396 489 AAG CTG CCC CTG Adequate Weak Weak ... codons are found in exons 1, 2, 3, 4¢ and 4, but only those in exons 3, 4¢ and are located in an adequate context for translational start (Table 2) Taken together, these data suggest that the smaller...

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Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf

Ngày tải lên : 20/02/2014, 02:21

... vitro transcription of these gene copies in PSG nuclear extracts is shown at the bottom and the quantiﬁed transcription levels as the percentage of tRNA1Gly -1 taken as 100, are indicated on the ... activity Evidently, the fractions were devoid of mutual contamination In every fractionation the quantities of fractions had to be optimized because use of larger amounts of any individual fraction ... the interaction of TFIIIB with tRNA1 -1 was Gly more stable than the interaction with tRNA1 -6,7 Quantiﬁcation of the ratio of heparin-resistant complexes to the TFIIIB ⁄ C–DNA complexes in the...

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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf

Ngày tải lên : 20/02/2014, 11:20

... transcriptase was omitted (data not shown) RT-PCR for b-actin was set up as an internal control to ensure equal loading and ﬁrst strand synthesis with forward primer, 5¢-ATGTTTGAGACCTTCAACAC-3¢, and reverse ... (Eur J Biochem 270) 2279 Fig Functional analysis of fgl2 promoter (A) Analysis of fgl2 RNA by semiquantitative RT-PCR RT-PCR was performed with lg of each RNA sample M, macrophages; M + MHV-3, macrophages ... However, when both threshold amounts (10 ng) of Sp1 and Sp3 were added together, with increasing quantities of Ets-1, a cooperative positive functional interaction was evident (Fig 9B) To deﬁne...

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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf

Ngày tải lên : 21/02/2014, 01:21

... or in the presence of sodium azide, an initial dilution of SOD was adjusted to give Vs equal to half Vb (equal to unit of SOD activity under standard conditions) After incubation of aliquots at ... with cultures grown to exponential phase in the absence of paraquat and IPTG (Materials and methods [24]) The ﬁnal concentration of paraquat and IPTG used for the experiment presented were chosen ... Anal Biochem 44, 276–287 43 Bradford, M.M (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilising the principle of protein binding Anal Biochem 72, 248–254...

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