... in MOPS buffer, and transferred onto a nylon membrane using an electrotransfer unit (Hoefer Pharmacia Biotech, San fransisco, CA) as described by Lewandowski and Dawson [46] Northern blot hybridization...
... employed the ABI automated dye-terminator system It was performed at a contract sequencing facility (Macrogen, Inc Seoul, South Korea) For each sequencing reaction, 50 ng template and 3.2 pmol primer ... Burden Of Hepatitis C Working Group.: Global burden of disease (GBD) for hepatitis C J Clin Pharmacol 2004, 44:20-29 Kim WR: The burden of hepatitis C in the United States Hepatology 2002, 36:S30-S34...
... employed the ABI automated dye-terminator system It was performed at a contract sequencing facility (Macrogen, Inc Seoul, South Korea) For each sequencing reaction, 50 ng template and 3.2 pmol primer ... Burden Of Hepatitis C Working Group.: Global burden of disease (GBD) for hepatitis C J Clin Pharmacol 2004, 44:20-29 Kim WR: The burden of hepatitis C in the United States Hepatology 2002, 36:S30-S34...
... has been described in previous studies [13-15,17,18] The bacterial DNA might be incorporated by macrophages, which are disseminated by the circulation and reach the joint due to an increased cellular ... infection with or without reactive arthritis Arthritis Rheum 1998, 41:855-862 Rahman MU, Cheema MA, Schumacher HR, Hudson AP: Molecular evidence for the presence of chlamydia in the synovium of patients ... Wang Z, Wang GF, El-Gabalawy H, Goldbach-Mansky R, Li Y, Majeed W, Zhang H, Ngai N, Hudson AP, Schumacher HR: Chromosomal DNA from a variety of bacterial species is present in synovial tissue from...
... Gagnon, T.H Thai, Pham Thi Tran Chau, N.T.Mai, L.N Dung and Laurent Chiche Solution Structure of the Macrocyclic Squash Trypsin Inhibitor MCoTI-II, the first member of a new family of cyclic Knottins ... and Le Nguyen D., V Squash trypsin inhibitors from Momordica cochinchinensis exhibit an atypical macrocyclic structure Biochemistry, V.39(2000), pp 5722-5730 Hioraki Inoue, Hiroshi Nojima and Hiroto ... T.C Pham, T H, Phan, J F Hernandez and Le nguyen, D Squash Inhibitors: From Structural Motifs to Macrocyclic Knottins, Current Protein and Peptide Science, V.5(2004), pp 341-349 11 Le nguyen, D.,...
... to ensure specificity in the amplification QPCR of biological samples was performed on a MX3000P machine (Stratagene, USA) and reactions containing synthetic templates were performed on a Rotorcycler...
... with a Storm 860Ò (Pharmacia Amersham, Uppsala, Sweden) using a chemi¯uorescent substrate ECF (Pharmacia Amersham) Data analysis was performed using IMAGE QUANTÒ software (Pharmacia Amershamm), giving...
... without clinical response Cancer Chemother Pharmacol 2000, 46(Suppl):S62-66 Oelke M, Kurokawa T, Hentrich I, Behringer D, Cerundolo V, Lindemann A, Mackensen A: Functional characterization of CD8(+) ... Nordblom GD, Das I, DeSilva BS, Khan MN, Bowsher RR: Validation of immunoassays for bioanalysis: a pharmaceutical industry perspective J Pharm Biomed Anal 2000, 21:1249-1273 Janetzki S, Schaed S, Blachere ... contribute to inter assay variability for the tetramer assay include cell preparation, staining methods, machine setting, gating during acquisition and data analysis Percent CV
... viral nucleic acids The detection was accomplished in hours using only a micro-centrifuge and PCR machine and did not require any virus-specific reagents The sensitivity is ~1000 genome equivalents/ml...
... viral nucleic acids The detection was accomplished in hours using only a micro-centrifuge and PCR machine and did not require any virus-specific reagents The sensitivity is ~1000 genome equivalents/ml...
... [Qiagen]) were evaluated Reactions were carried out in a 25-μl volume on an MX3005P real time PCR machine (Stratagene) Quantitative analysis For preparation of standard controls, the cloned H5 gene...
... nitrogen with a pestle and mortar The total RNA extractions were performed from 100 mg of each macerate plant tissue in liquid nitrogen, using Invisorb Spin Plant RNA Mini kit (Invitek) according ... a no template control in parallel for each gene The melting-curve analysis performed by the PCR machine after 40 cycles of amplification and agarose gel electrophoresis showed that all the primer...
... previously [12] Leaf and sprout samples were directly used for extracting tissue sap All samples were macerated in extraction bags (Bioreba, Reinach, Switzerland) by pounding the tissue with a hammer ... absorbance of the healthy control was < 0.030, a positive threshold of 0.100 was used RT-PCR From each macerated sample, 100 μl of sap was extracted with the Tri-Reagent (Molecular Research Center, Inc,...
... Real-time RT-PCR was performed using iScript™ OneStep RT-PCR Kit Probes in Chromo real time PCR machines (Bio-Rad, Hercules, CA, USA) The reaction was conducted in a total volume of 25 μl containing ... Research and Development, Ministry of Health, Jakarta, Indonesia for his support for this work, and Dr Maciej Boni from the Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 ... Thailand, 2004 Emerg Infect Dis 2005, 11(11):1664-1672 Boltz DA, Douangngeun B, Sinthasak S, Phommachanh P, Rolston S, Chen H, Guan Y, Peiris JS, Smith JG, Webster RG: H5N1 influenza viruses in...