... number of critical media analysts, and is associated with the work of Noam Chomsky and Edward Herman Their ‘propaganda model’ identifies a number of ‘filters’ that shape what we see and hear on the ... newspapers and magazines, 130 radio stations and 100 online sites in Ireland, the United Kingdom, South Africa, India, Australia and New Zealand In Ireland, it owns seven national and 17 local ... than any other bank the excesses ofthe Celtic Tiger and property lending.47 He was also vice-chairman of Aer Lingus (national airline), chairman of Aon MacDonagh Boland Group (insurance company)...
... Iraq in the name of Ba’athism Aflaq was invited to settle in Iraq, accepted the offer, and eventually became the leader ofthe National Command ofthe Iraqi Ba’ath Party African Union (AU) The ... Located on the offshore island ofthe same name, the Emirate of Abu Apoliticaland economic dictionary ofthe middle east Dhabi was founded by members ofthe Ahl bu Falah clan ofthe Bani Yas ... 32 provinces (velaya, plural velayat): Badakhshan, Badghis, Baghlan, Balkh, Bamian, Farah, Faryab, Ghazni, Ghowr, Helmand, Herat, Jowzjan, Kabol, Kandahar, Kapisa, Khowst, Konar, Kondoz, Laghman,...
... his pajamas, and approach the bed “What on earth is the matter?” he said He also laid hands on Maria, and, at his touch, she became able to move “What on earth is the matter?” he asked again “She ... regarded the whole as the antics of infancy The Lees lived near the Edghams, on the same street, and Mrs Lee and Aunt Maria had exchanged several calls They were, in fact, almost intimate The Lees ... Queen Anne cottage ofthe better class, situated in a small lot of land, and with other houses very near on either side There was a great clump of hydrangeas on the small smooth lawn in front, and...
... Eastern part ofthe nature reserve called “Dvorčák” A quality oak stand with Fagus sylvatica and Quercus petraea grows on a gentle SE–S slope, sporadically with gaps European ash and small-leaved ... 11.71 as an evidence ofthe natural structure of forests ofthe Moravian Gate Fagus sylvatica with interspersed Tilia cordata, Quercus petraea, Carpinus betulus and Acer pseudoplatanus predominate ... terminated by the Maleník plateau The highest peak in the management-plan area (MPA) is Maleník with its height of 479 m Mean altitude a. s.l ranges between 300 and 400 m The lowest point ofthe MPA...
... basement geology consisted of alternating beds of sedimentary sandstone and mudstone ofthe Palaeogene system [4] The mean annual precipitation and annual mean air temperature were 1134 mm and ... Japan, experimental text ofthe geological map of Japan, Iwamizawa, Hokkaido Development Agency, Sapporo, Japan, 1964 (in Japanese) [5] Higashiura Y., Larval densities anda life-table for the ... leaves that open early within a year, and LB2 approximates the amount of leaves that open late To express the relative importance of leaves that emerge late in the summer for annual leaf production,...
... Grammartical Structures ofthe equivalent translation The target language is English andthe source Adjective Warm language is Vietnamese The data are classified into semantic and pragmatic features ... hyponym of animal 2.2.1.7 Semantic field The semantic structure of vocabulary ofa language can be studied in a precise and systematic way by means of componential analysis of which the theory of ... nation, andthe administration and control of its internal and external affairs 3.1 RESEARCH DESIGN AND METHODOLOGY This study is intended to follow quantitative and qualitative approach and develops...
... important and significant characteristics ofthe WDBs As a result, the topic A Contrastive animals that nature has provided to feed both our body and spirit As Analysis ofthe Semantic and Pragmatic ... fields as well as pragmatic features ofthe WDBs The finding ofthe pragmatic features ofthe WDBs leads both teachers and learners of study may be in one way or another beneficial to the language ... the fact that - Syntactic and cultural features ofthe WDBs learners generally impose the use of their culture on that ofthe target - Semantic and pragmatic features ofthe idioms language is very...
... observation, as well by the peasant as by the man of erudition The fact, that man, "made of one blood, can dwell" in all the varieties of climate, "on the face ofthe whole earth," and can sustain ... gratification of appetites, regardless of others, is the strongest feature of barbarism We see then, even as a dictate of refinement, that the use of tobacco should be abandoned; and it has been abandoned ... charge ofthe patient She was much emaciated The discharge from the bowels continued unabated, and was often attended with severe pain and great prostration of strength The salivation was accompanied...
... 5¢-GTTCACCACCAGAAGCT GGTGTTCTTCGCTGAAGACGTGGGTTCTAACAAG GGTGCT-3¢; Abc, 5¢-CACAACGCCACCAACCATCAGA CCGATGATAGCACCCTTGTTAGAACCCAC-3¢; Abstart, 5¢-GCGTAGGGTCGACATATGGACGCTGAATT CCGTCACG-3¢; Abstop, ... lanes containing Ab(1–42) and Ab(M1– 42), there were prominent bands at approximately kDa and faint bands at approximately 14 kDa The band at approximately 14 kDa is not an impurity as it was ... 5¢-CCTGCCGAGCTCCTATTA CACAACGCCACCAACCATCAG-3¢ The PCR solution was prepared in the buffer supplied with the enzyme, and contained Aba, Abb and Abc at 40 nm each, andthe start and stop primers Abstart...
... GCT N AAC K AAG A GCG N AAC E GAG Y TAC D GAC K AAG L CTA K AAG Q CAG L CTC G GGC G GGG E GAG E GAG L CTG K AAG T ACC K AAG P CCC V GTG S AGC T ACC T ACA G GGT L CTC R CGC S TCG M ATG K AAG A GCC ... STQLPSDEPLNGLNDKAIQDILNEHNMFRAKEHVPPLTWNTTLA 44 Hordeum MQTPKLVILLALAMSAAMVNLSQAQNSP YVSP AA AVG.GAVS.S.K.Q 54 Triticum MQTPKLAILLALAMSAAMANLSQAQNSP Y.SP AA AVG.GAV S.K.Q 54 Zea -MAPRLACLLALAMAAIVVAPCTAQNSP ... Q CAG T ACC Q CAA K AAG P CCG K AAG S TCC H CAC W TGG M ATG ACG C TGC G GGT E GAG T ACC E GAA A GCC L CTC K AAG G GGC Y TAC K AAG I ATC B Cyn d 24 CGT I ATC G GGA A GCG R AGG G GGA I ATA T ACG...
... in the pH 4.2– 8.0 range, anda marked increase in Km at alkaline pH (Table 2, Fig 3) The D215N and D140N mutants displayed an acidic shift in the pH-activity pro les, whereas the Y214F andthe ... The pKa of Asp140 is much lower than that of Asp142 and Glu144 in all situations where all three residues are present andthe one proton shared by Asp140 and Asp142 appears to remain on Asp142 ... functionality of such a triad is affected by the surrounding residues The results so far indicate that larger parts ofthe polar core ofthe catalytic TIM barrel of family 18 chitinases play a role...
... validate the mechanism of induction of GAL genes by galactose In each ofthe models, cytoplasmic Gal3p is activated by galactose Further, Gal4p dimerizes and interacts with the DNA to form the DNA–Gal4p ... Gal3p based on translocation and dimerization possibilities of Gal3p The steady-state response analysis rules out dimerization or translocation of Gal3p Further, the analysis clearly demonstrates ... concentrations are dependent on the status ofthe switch Thus, to account for autoregulation of Gal80p and Gal3p, the total concentrations of these were related to the translation status of genes...
... (5Â-GCACCTGTATGGTCGTAGTGAGCCGCAGTAAG CAGC-3Â) For the Q116N mutant forward and reverse: (5Â-GATCTTGCTGCTTACTAACGCTCACTACGACC ATACAGG-3Â) (5Â-GCACCTGTATGGTCGTAGTGAGCGTTAGTAAG CAGC-3Â) For the Q116H mutant ... forward and reverse: (5Â-GATCTTGCTGCTTACTCATGCTCACTACGACC ATACAGG-3Â) (5Â-GCACCTGTATGGTCGTAGTGAGCATGAGTAA GCAGC-3Â) Production and purication ofthe zinc b-lactamase LB medium (100 mL) containing ... Metallo-b-lactamase GOB-1 L E Horsfall et al 12 Bandoh K, Muto Y, Watanabe K, Katoh N & Ueno K (1991) Biochemical properties and purication of metallo-beta-lactamase from Bacteroides fragilis Antimicrob...
... on catalytic rates and relative efficiency (Table and Fig 2) Previous work has shown that inactivation ofthe reactive cysteine produces a dramatic reduction in Vmax and kcat for a variety of CKs ... reverse CCC AGG TTG AAT AGT GAA CAG ACG GGC TGG CTC AGG CAG TCT CCT ATG TTC AGA TGG GCC TTC GCC TCC GG GTC CTG ATC CTG CTC CTA CCC GGG CCC GGG CCC GGG CAG GAC CAG GAT TAG GAG TAA GTG CAA GTC CAA GTC ... sites, essentially allowing only one ofa set of interacting active sites to complete a catalytic cycle at a time Further understanding of catalysis andthe interaction of active sites in these unique...
... 5¢-ATGGCACCAAAAGCAAAAATCG-3¢ and reverse 5¢-AGCTAATGCCTTCATTCTCTTAG-3¢; Pf MQO forward 5¢-ATGATATGTGTTAAAAATATTTTG-3¢ and reverse 5¢-TCATAAATAATTAACGGGATATTCG-3¢) Both PCR and RT-PCR products were cloned ... evaluation of Vmax and kcat values for malate/NAD and OAA/NADH (Table 1) The saturating concentrations of OAA and NADH were found to be 250 lM and 150 lM, respectively, while for malate and ... of at least three observations Substrate/cofactor Reduction (pH 7.5) Oxaloacetate (NADH) NADH (OAA) APADH (OAA) a- Keto-malonate (NADH) Oxidation (pH 10.2) Malate (NAD) NAD (malate) APAD (malate)...
... [22] The oligonucleotides AOX-F (5¢-GCGACTGGTTCCAATTGAC-3¢), AOX-R (5¢-GG TCTTCTCGTAAGTGCCC-3¢), SKTM -A6 3V (5¢-GAC AAATACTCTGAAGTACTCAAAGATGCCCAG-3¢), SK TM-1R (5¢-CTGGGCATCTTTGAGTACTTCAGAGTA TTGTC-3¢), ... or activates the actomyosin ATPase in the absence and in the presence of calcium, respectively All mutant Tms were able to regulate the ATPase activity by Ca2+, andthe regulation was cooperative ... mutation also introduces changes in the surface charge of Tm All the mutant amino acids are involved in interchain and intrachain interactions and therefore are important for the stabilization of the...
... specific probe was labeled and purified as described above A kb human b-actin cDNA fragment was used as the control probe and labeled with Ready-To-GoTM DNA Labelling Bead (–dCTP) (Amersham Pharmacia ... as a probe The antisense single stranded DNA probe was synthesized using the Strip-EZTM PCR kit (Ambion, Austin, TX, USA), in the presence of antisense primer SB1-20 (5¢-TC AAACCACACCCCGAGAAA-3¢) ... Cloning and characterization of Na+ channel b1B (Eur J Biochem 270) 4763 reaction (RT-PCR) and RACE-PCR Marathon-ReadyTM human adrenal gland and fetal brain cDNA libraries were purchased from...
... (Tables and 2) revealed the same linkage pattern and sugar sequence as in the polysaccharide of P penneri (structure 1) and one additional sugar residue (a- GlcpII) attached at position of GalNAcI ... polysaccharide has a tetrasaccharide repeating unit containing one residue each of D-Glc and D-Gal and two D-residues of GalNAc The 1H- and 13C NMR spectra ofthe polysaccharide were assigned using ... serological assays Polyclonal O-antisera were obtained by immunization of rabbits with heat-inactivated bacteria of P penneri and according to a published procedure [11] Agglutination and precipitation...
... generated by a crystallographic two-fold The data and refinement statistics are shown in Table The Ramachandran plot has 96.9% ofthe residues in the most favored region and 3.1% in the additional ... dehydrogenase and prephenate dehydratase catalyze the biosynthesis of tyrosine and phenylalanine, respectively As this biosynthetic pathway is absent in mammals but is essential for the survival of bacteria ... oligonucleotide pair 2: 5¢-GCTACGTTTAAAGCGATGATGAACCTGGAAATG CTCGAGTCC-3¢ (forward primer with DraI site underlined) andthe same reverse primer Oligonucleotide pair for amplification of MT0975 (217 amino acid...
... 5¢-GGTCTCTCTGCTGCCGGAAATG-3¢ 5¢-CATGGCTTAATCTTTGAGAC-3¢ 5¢-GCTCTCATGCTCTTGCCAAAAC-3¢ 5¢-CGTATCGCATTTCGCTGCGTTC-3¢ 5¢-CTCACTACCAAACAGAATGTTTGAGAAGG-3¢ 5¢-GTTCGCCTAGACGCTCTCTTC-3¢ 5¢-GCCGCTTCACTCGCCGTTACTAAGGC-3¢ ... 5¢-GGATCCATGGGC AAACGCGGGAGCC-3¢ and 5¢-ATCGATGTCGACTCA TTTTTCTCCAGTAATGAAGAG-3¢ The gene was cloned in fusion with yEGFP3 using BamHI and SalI sites of pUG34 This vector was cleaved with XbaI and ... processing A ProtA TE A ProtA-Utp25 W B TE FT W B AD-Sas10 35S DA A AD-Mpp10 23S A0 A1 DA A 22S A1 23S 22S/21S 20S DA A 21S A1 BD-Utp25 A0 A1 FT M B Goldfeder and C C Oliveira AD-Imp4 AD U3 DA AD-Imp3...