... fraction (LV-EF) was calculated by the modified Simpson´s method LA and LV diameter were measured by M-Mode echocardiography High grade cardiac valvular disease was excluded by Color, PW and CW ... differences between basal, midventricular and apical myocardium (see Table 3) Table 3: Tissue velocities (S´, E´, A´) of basal, mid and apical segments as assessed by VVI S´ (cm/s) E´ (cm/s) Basal 3.64 ... and strain rate measurements by cardiac ultrasound: principles, implementation and limitations Eur J Echocardiogr 2000; 1(3): 154-170 Mirsky I, Parmley WW Assessment of passive elastic stiffness...
... tagging a tropospheric particle that has got a high PV value by diabatic PV production in cloudy areas as a stratospheric-origin particle Once a particle has gone across a boundary limit of the ... corresponding climatologies As expected, results show again elevated ozone overpassing the climatological value by more than 40 ppbv over the heat wave period, as well as the change in the vertical ... sources to the measured CO mixing ratios, with a possible impact on ozone production 5.3 Biomass burning CO emissions Major source regions of biomass burning in Southern Europe as well as of Saharan...
... such as Asp12, Glu50, Asp72 and Glu133, are clustered in the vicinity of Asp136 It has been reported that the corresponding mutation (Asp134 fi His) stabilizes Ec-RNase HI by 7.0 °C in Tm as a ... thermophilus RNase HI (Tt-RNase HI) Gly39 and Gly97 are conserved as Gly41 and Gly100 in Tt-RNase HI, respectively Val76 is conserved as Val74 in Ec-RNase HI Asn90 is conserved as Gln96 in RNase HI from ... encoding R97G-RNase HI, D136H-RNase HI, 5·-RNase HI and 6·-RNase HI were constructed by sitedirected mutagenesis using PCR as described previously [33] Plasmid pET500M or pET500M4x was used as template...
... sulphate was converted to l-canaline by arginase treatment, essentially as described previously [61] The arginase required for this was obtained as a crude extract from the leaves of Canavalia brasiliensis ... the native protein) in the soybean arginyl-tRNA synthetase was not targeted by this protease The position of the cleavage was confirmed by N-terminal protein sequencing The results reported here ... reverse transcriptase (Invitrogen, Karlsruhe, Germany) Sequences were identified by blast comparison (http://www.ncbi.nlm.nih.gov/blast/Blast.cgi) Gene for arginyl-tRNA synthetase The gene for...
... When chitinase A was incubated with (GlcNAc)2, neither a decrease in (GlcNAc)2 nor an increase in GlcNAc was observed upon incubation up to 57 h (Fig 3A) In contrast, when (GlcNAc)3 was the substrate, ... sample was subjected to HPLC-MS The signal was recorded in the single ion mode set for the masses 222, 425, 628, 831, 1034 and 1237 The relative intensity of the base peaks is plotted as a function ... mm was determined after of reaction Given the fact that chitinase A produced (GlcNAc)2 as the major end product, the initial velocity of all the substrates was calculated based on the release...
... Protein was determined with the RotNanoquant-Microassay from Roth (Karlsruhe, Germany) using bovine serum albumin as standard Assay to determine the stereospecificity of F420H2 oxidase The assay ... trifluoroacetic acid (TFA) The column was then washed with 0.1% (v ⁄ v) TFA to remove salts and was then eluted with 84% (v ⁄ v) acetonitrile ⁄ 0.1% (v ⁄ v) TFA The eluate was dried by vacuum centrifugation ... F420-dependent reductase (Mer) [15]; F420H2 dehydrogenase complex (Fpo) [15]; F420H2 : NADP+ oxidoreductase (Fno) [17,18]; and F420-dependent glucose-6-phosphate dehydrogenase (Fgd) [6] Adf, Mer...
... as the basis for designing ESP courses (Dudley-Evans, 1991) However, this approach has received major criticism for being 19 inflexible The initial Target-Situation Analysis model by Munby was ... setting was perceived as the greatest difficulty Based on the needs analysis, a course on Speaking and Listening in a Health-Care Setting was developed to respond to what was identified as students' ... Needs Assessment, Target Needs Analysis ESP TARGET SITUATION NEEDS ANALYSIS: THE ENGLISH LANGUAGE COMMUNICATIVE NEEDS AS PERCEIVED BY HEALTH PROFESSIONALS IN THE RIYADH AREA by MAJID ALHARBY B.A.,...
... represented by red spheres Hydrogen bonds and salt bridges are depicted by dashed red lines (A) pVIPR-U5 drawn in purple as presented by B*2705 (B) pVIPR-U5 drawn in yellow as presented by B*2709 ... B*2705) is strongly associated with AS, whereas another, HLA-B*2709 (in short, B*2709) is not [2,3,11] The proteins encoded by these two alleles differ only by a micropolymorphism (Asp116 in B*2705 ... conformation by B*2709, but in an exceptional dual conformation by the AS- associated B*2705 subtype One of the conformations observed in B*2705:pVIPR is identical to that seen in B*2709, whereas in...
... across at least some asset classes However, some studies suggest that credit ratings have not historically been comparable across asset classes • Increasing transparency may be more feasible and ... terminology that applies across at least some asset classes, the staff found that credit ratings historically have not been comparable across asset classes and it may not be feasible to attain this comparability ... methodologies for specific asset classes Standardized scenarios, however, could require the creation and mapping out of thousands of assumptions to achieve relevancy for each asset class, industry, geographic...
... document is to pass along tips we learned that may be useful as you consider adopting QualysGuard PC Objective My goal was to get our systems into a “steady state” as quickly as possible to meet ... priorities by weighing best practices such as NIST and CIS QualysGuard PC can also help Are all technically feasible controls defined inside the reporting template policy based on current paper-based ... clean and easy to read Compliance data was delivered on time and closed audits faster Moreover, the cost of the new solution was 90 percent cheaper than the previous platform and it worked as promised,...
... supply of bozal negroes CHAPTER I 10 [Footnote 12: Las Casas, Historio de las Indias (Madrid, 1875, 1876); Arthur Helps, Life of Las Casas (London, 1873); Saco, _op cit_., pp 62-104.] The crown ... Rum as much as possible and sell as much by the short mesuer as you can." And again: "Order them in the Bots to worter thear Rum, as the proof will Rise by the Rum Standing in ye Son."[28] As ... considered as champions of rival faiths, the pagan blacks came increasingly to be reckoned as having no religion and therefore as a mere passive element ready for christianization As early as 1510,...
... its Purposes and Procedures Manual as a rating organization designated as an NRSRO by the SEC which has applied to, and whose NRSRO status has been confirmed by, the SVO Among other things, the ... question on a case -by- case basis But through the subsequent no-action letter process, Commission staff developed a number of objective criteria for assessing national recognition As a result, under ... Initiatives Broad-Based Commission Review The issues addressed by the Staff Report, as well as the study required by the Sarbanes-Oxley Act, were consistent with work previously undertaken by the In addition,...
... ppm pH Exchang Cab Exchang Mg Exchang K Extract Al ppm ZT CT Past ZT CT Past ZT CT Past ZT CT Past ZT CT Past ZT CT Past ZT CT Past 5᎐10 10᎐15 15᎐20 3.87 bA 2.70 cA 6.60 aA 17.67 aA 21.50 aA ... copper, iron, lead and manganese Žmgrkg a Element Treatment Depth Žcm 0᎐5 As ZT CT Past ZT CT Past ZT CT Past ZT CT Past ZT CT Past Pb Cu Fe Mn 5᎐10 10᎐15 15᎐20 0.058 aA 0.059 aA 0.087 bA 0.943 aA ... conditions As a general view, Fergusson Ž1991 pointed out that levels of extractable trace elements not always can be explained as a function of changes in soil properties This was the case in this...
... the march as Seen by a Private Soldier, by Warren Olney was such, that he did not make more than half the distance, and had to go into camp for the night Saturday was a reasonably pleasant day, ... division It was so far away as not to be in easy supporting distance On April 1st, our division was marched to an open field, and there carefully reviewed by General Grant This was our first ... bayonet of my gun was shot off, but possibly that was done by some man as Seen by a Private Soldier, by Warren Olney 11 behind me, firing just as I threw the muzzle of my gun into his way I didn't...
... (273–293 K) and faster (293–323 K) phases of band shift (Fig 2A); however, the 889 nm complex shows monophasic behavior (273–303 K; Fig 2B) with a slope comparable to the faster phase of the 915 ... temperature increase below 293 K, and the shift is faster and shows linear temperature dependence above 293 K In addition, the decrease in fluorescence inten- Fig Fluorescence emission spectra recorded ... feature and the highly symmetric ring assembly of BChls in LH1, as well as the interaction between BChl a and carotenoid molecules, have been confirmed [13] It has been shown that spirilloxanthin...
... transfer (FRET)-based assay of the redistribution of PHD fusion proteins from plasma membrane to cytosol was found to be a more reliable and quantitative method for measuring increases in cellular ... basal salt saline (BSS)] In the presence of mm extracellular calcium, mAChR activation induced both a transient and a sustained increase in intracellular calcium, as measured by Fura ⁄ AM-based ... was replaced with normal BSS containing CCh, which induced a second calcium increase and a further increase in Ins(1,4,5)P3 (Fig 3Aa) Both of these calcium increases were accompanied by increased...
... ⁄ P) [15] Reduction of |q| upon increase of |r| was primarily associated with a decrease of the optical factor, which for the pitch angle 50° was as low as 0.12 However, electron microscopy data ... standard method of lyses by alkali [44] was used for plasmid harvesting and purification Phenol–chloroform extrac- FLD technique The signal of linear dichroism was measured on JASCO J715 dichrograph ... studied by FLD A B Fig Mechanisms of action of enzymes of DNA topoisomerization (A) Topoisomerase I-3¢ (B) Topoisomerase II Two possible ways of action of topoisomerase I are shown, see text, as well...