... (upper panel) and nondenaturing PAGE (lower panel) analyses of the E78H ⁄ D164N mutant and related proteins Lanes a, the purified 38-kDa mature form; lanes b, the isolated 19-kDa propeptide; lanes ... 57-kDa band diminished whereas the 38-kDa and 19-kDa bands were enhanced (Fig 3A, lane c) These observations suggest that, at pH 7.0, the 38-kDa and 19-kDa proteins can be reversibly ligated with ... extract of transformant cells overexpressing the E78H ⁄ D164N mutant showed that the expressed product gave two major protein bands with molecular masses of 38 kDa and 19 kDa (Fig 2A), along with a...
... the wild-type enzymes and the various mutants from the two organisms (B stearothermophilus [47] and T brucei) are summarized in Table With all mutants, and for both organisms, a decrease in kcat ... quenching, tryptophan was excited at 295 nm to avoid phenylalanine and tyrosine fluorescence Excitation and emission spectra were recorded between 310 and 360 nm with excitation and emission slits ... For the trypanosome enzyme, Km for 1,3-BPGA and GAP increased significantly in the Pi mutant; in the Ps mutant, Km for GAP increased when the Km of 1,3-BPGA stayed constant This unchanged Km parallels...
... Myotoxins and DM64 were used as controls Statistical analysis Results represent mean ± SEM (n ‡ 4) Data were statistically evaluated by Analysis of Variance (ANOVA), followed by Newman-Keuls-Student’s ... jararaca venom and its inhibition by DMs Lane 1, molecular mass markers; lane 2, fibrinogen control; lane 3, fibrinogen + SVMP; lane 4, SVMP; lane 5, fibrinogen + (SVMP + EDTA); lane 6, fibrinogen ... response, such as antibodies, T-cell antigen receptor and histocompatibility antigens [52] In spite of the structural similarities between DM43 and DM64, the latter does not present any antihemorrhagic...
... determination of the crystal structure of cytochrome c6 and comparison with plastocyanin Structure 3, 1159–1169 Kerfeld CA, Anwar HP, Interrante R, Merchant S & Yeates TO (1995) The structure of chloroplast ... solvent volumes in structures deposited in the Protein Data Bank is astonishingly high Searching the Protein Data Bank for protein structures with VS between 5.00% and 17.57% gave 29 structures Calculations ... adjacent to the second heme ligand, Met65, and Pro66 undergoes conformational changes upon oxidation ⁄ reduction [16] Whereas in cyanobacterial, plant and red and brown algal cytochrome c6 this...
... Basic Structured Grid Generation withan introduction to unstructured grid generation Basic Structured Grid Generation withan introduction to unstructured grid generation M Farrashkhalvat and ... the transformation rules (1.80) and (1.82) that the quantities k T.k and Tk.k are invariants Given two vectors u and v, second-order tensors can be generated by taking products of covariant or ... the definitions (1.120) and (1.122) and the transformation rules (1.70), (1.72), and (1.109), show that ui and ui,j satisfy the transformation rules for ,j mixed and covariant second-order tensors,...
... diagnosis and management of the patient, drafting of the manuscript, literature search, and submission and revision of the manuscript RM and SM were responsible for drafting of the manuscript and literature ... microorganisms Our patient was treated with antibiotics and heparin for two days After obtaining BAL culture and lung scan results, however, treatment was discontinued He recovered spontaneously ... during extracorporeal shockwave lithotripsy Anesth Analg 1987, 66:S178 Chen H, Wang Z, Ning X, Xu H, Xiao K: Animal study on lung injury caused by stimulant segmented shock waves Chin J Traumatol...
... diagnosis and management of the patient, drafting of the manuscript, literature search, and submission and revision of the manuscript RM and SM were responsible for drafting of the manuscript and literature ... microorganisms Our patient was treated with antibiotics and heparin for two days After obtaining BAL culture and lung scan results, however, treatment was discontinued He recovered spontaneously ... during extracorporeal shockwave lithotripsy Anesth Analg 1987, 66:S178 Chen H, Wang Z, Ning X, Xu H, Xiao K: Animal study on lung injury caused by stimulant segmented shock waves Chin J Traumatol...
... 0), |N, kx > and |N ′ , kx + q⊥ > are electron states before and after scattering; ωq is the energy of an acoustic phonon with the wave vector q = (q⊥ , qz ); a+ N,kx and aN, kx (b+ q and bq ) are ... PARABOLIC QUANTUM WELLS 203 A = ξ /2ρvs with vs , ξ and ρ are the sound velocity, the deformation potential constant and the mass density, respectively; kB is the Boltzmann constant; Lx and εF are ... m and e are the effective mass and the charge of a conduction electron, respectively, k⊥ = (kx , ky ) is its wave vector in the (x, y) plan; z0 = ( kx ωc + eE1 )/mωp2 ; ωp2 = ωz2 + ωc2 , ωz and...
... associated with urinary incontinence and a post-ictal confusional state The falls associated with three episodes resulted in trauma to the head, face and arms She underwent an extensive neurologic and ... Evaluation and outcome of patients with syncope Medicine (Balt) 1990;69:160-75 12 Day SC, Cook EF, Funkenstein H, Goldman L Evaluation and outcome of emergency room patients with transient loss ... 2009, anti-seizure medications, and normal cardiac evaluations patients were referred to our center for further evaluation It was only following prolonged cardiac rhythm monitoring withan implantable...
... Daniella, Steven,…and their partners; Roos, Rolnan, Cornelie, Judith and Wendy from the Construction and Transport groups; Our secretaries: Anke, Joke and Ellen; Yan, Dong, Ping, Chang Wei and ... Integrated management and policy analysis Integrated management Rapid changes of objectives and methodological approaches towards the management of natural resources and environment can be observed ... climate change (Dowlatabadi, 1995; Hulme and Raper, 1995; Janssen and de Vries, 1998) and natural resources and environmental management (Stephens and Hess, 1999; Turner, 2000; De Kok and Wind,...
... lines of parameters and one line for messages The parameters themselves are organized in a tree-like structurewith up to levels You can change the various parameters and functions using the ... parameter mode, select the relevant parameters with the left and middle knob Press the EDIT button and change the selected value with the right knob EDIT STORE Discard the change by pressing the EDIT ... be guaranteed! A complete description of the power-on process can be found in the dyno description MANUAL APA ON/OFF Press the MANUAL button to change to the MANUAL operating state The transition...
... string to another • More about strings and string functions today 6.087 Lecture – January 15, 2010 Review Pointers and Memory Addresses Physical and Virtual Memory Addressing and Indirection Functions ... ,c ); • Many other utility functions exist 20 6.087 Lecture – January 15, 2010 Review Pointers and Memory Addresses Physical and Virtual Memory Addressing and Indirection Functionswith Multiple ... 12 17 6.087 Lecture – January 15, 2010 Review Pointers and Memory Addresses Physical and Virtual Memory Addressing and Indirection Functionswith Multiple Outputs Arrays and Pointer Arithmetic...
... was kept constant with approximately 12 watts maximum output The solution pH value was kept manually constant at desired levels with NaOH and H2SO4 solutions The Red 141 and H2O2 and other chemicals ... Korper, Annalen fur Physik, 1, 486 Oppenlander, T and Gliese, S (2000) Mineralization of organic micropollutants (homologous alcohols and phenols) in water by vacuum-UV-oxidation (H2O-VUV) withan ... (Gonzalez and Braun, 1995) within a photochemical reaction zone of less than 0.1 mm (Heit and Braun, 1997) The UV light with high energy causes the homolysis of water into hydroxyl radicals and hydrogen...
... British or European Standard), and certification standards and procedures to test and analyse PCM systems for heating/cooling applications, energy demand management within the built environment ... pipe is 22mm and the total length of the heat exchanger is 5.73m with turns The inlet and outlet of the heat exchanger are connected, respectively, to the outlet and inlet of the manifold at the ... Technologies, and Head of Department of Architecture and Built Environment, University of Nottingham and he has been named as an inventor on more than 20 international patents and awarded Distinguished/Honorary...
... CURLOPT_RETURNTRANSFER, 1); // Return stream contents curl_setopt($ch, CURLOPT_BINARYTRANSFER, 1); // We'll be returning this transfer, and the data is binary $data = curl_exec($ch); // // Grab the jpg and ... company to company // Please your Read your Credit Card Processing company Manual $xml =" GOOD PREAUTH ... CODE1001 web Muhammad Imran 4673 Blue Street Los Angeles...
... the time hang up hang up & hangs up hanging up up up hang up (on) p.v When you stop talking on the telephone and put down the receiver, you hang up the telephone When you are angry and hang up the ... childish and immature You're acting like a baby Why don't you grow up! When he put his fist through the wall, I said, "Oh, grow up!" hand out hand out & hands out handing out handed out handed out hand ... ahead go ahead (with) p.v When you take an action you have already considered doing, you go ahead with the action I've decided to go ahead with my plan to reorganize the company After she learned...
... very kind and generous He _ helping other people 42 FOCUS ON: phrasal verbs with get, Many phrasal verbs are based on the verb get, and it is important to understand that the meaning of ... hotel and arranged for a room yet What hasn't Mr Baker done yet? 15 Hank bought a new CD player and connected the plug to the outlet What did Hank do? 16 In Question 15, how would you describe Hank's ... your car's gas tank with their gas, you'd get better mileage and a cleaner engine 321 13 We're planning to for a few weeks Would you mind watering our plants until we return?...
... performing financial wizardry with Excel You learn techniques and functions for amortizing loans, analyzing investments, dealing with bonds, and using discounting for business case and cash-flow analysis ... values and formulas in a range with the fill handle, you can use either of the following two techniques: ■ If you want to clear only the values and formulas in a range, select the range and then ... showing you the very handy drag-and-drop method Using Drag-and-Drop to Copy a Range If you have a mouse, you can use it to copy a range by selecting the range and then clicking and dragging it to...