vivo in human cell lines that were either transiently transfected or stably transfected with the hiv 1 ltr
Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
Ngày tải lên :
19/02/2014, 05:20
... HO -1 Incidentally, the three cell lines with low HO -1 expression, K562, Jurkat, and H146, were maintained in suspension culture (Fig 1) , although HO -1 is expressed in two other cell lines, YN -1 ... analysis HeLa cells, which were left untransfected (Control) or transfected with the indicated siRNA, were cultured for 12 h, and then treated with actinomycin D (AMD) (1 lgÆmL )1) for the indicated ... left untrasnfected or transfected with siHO-2, and incubated for 12 h, followed by the addition of actinomycin D (1 lgÆmL )1) [37] The cells were further incubated for up to h, and then harvested...
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Báo cáo khoa học: Internalization of cystatin C in human cell lines docx
Ngày tải lên :
16/03/2014, 06:20
... Biochem J 211 , 12 9 13 8 30 Lenney JF, Tolan JR, Sugai WJ & Lee AG (19 79) Thermostable endogenous inhibitors of cathepsins B and H Eur J Biochem 10 1, 15 3 16 1 Supporting information The following supplementary ... and cathepsin B, because of the removal of side chains involved in the interaction with these enzymes [ 21] The other, N39K–cystatin C, lacks inhibitory activity against the C13 family cysteine ... cystatin CÆmg cell protein )1, within the cells By contrast, the cystatin C concentration in the medium increased from 15 to 45 ng cystatin CÆmg cell protein )1 (Fig 4), as expected for a protein...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
Ngày tải lên :
23/03/2014, 07:20
... 1. 15 .1. 1), glutathione peroxidase (GPX, EC 1. 11. 1.9) and catalase (EC 1. 11. 1.6), and low molecular weight antioxidants, such as vitamins and various biological dyes, can reduce ROS in vivo [16 19 ] ... lgÆmL )1 for 15 Samples were added to the reaction mixture containing HBSS (pH 7.4), 10 lm scopoletin and 50 lgÆmL )1 horseradish peroxidase in a 96-well plate The decrease in scopoletin fluorescence ... the attached cells The cells were then washed gently with NaCl ⁄ Pi three times and permeabilized with mL of 0 .1% Triton X -10 0 in NaCl ⁄ Pi in each dish for 10 After permeabilization, cells were...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
Ngày tải lên :
23/03/2014, 09:21
... isolated from stably transfected 293 cell pools that had been cultured with or without lgÆmL )1 doxycycline for 20 h The RNA blot was first hybridized with a human OCT1 probe Without stripping, the blot ... expression in 293 cells by flow cytometry 293 cell pools stably transfected with either pEBTet ⁄ eGFP or pEBTetD ⁄ eGFP were cultured with or without lgÆmL )1 doxycycline for 20 h, resuspended, and then ... eGFP -transfected cells, the median fluorescence intensity was 19 5 in the off-state and 18 60 in 786 Doxycycline – Relative background transcription (%) 0. 014 0. 014 0.0 011 0.0052 0.045 0.0 017 0.068...
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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
Ngày tải lên :
19/02/2014, 06:20
... mRNAs (A) Northern blot analysis YN -1 cells were cultured for 12 h under normoxia or hypoxia (1% oxygen), and then treated with or without actinomycin D (AMD) (1 lgÆmL )1) for the indicated number ... mRNA under normoxia or hypoxia The intensity representing HO -1 or HO-2 mRNA was normalized with respect to the intensity of b-actin mRNA The intensity representing HO -1 or HO-2 mRNA at the time ... quantified, and the intensity representing HO -1 or HO-2 mRNA was normalized with respect to the intensity for the 28S RNA signal The intensity representing HO -1 or HO-2 mRNA at the time (0 h)...
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Báo cáo sinh học: "Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets" doc
Ngày tải lên :
18/06/2014, 19:20
... is in agreement with previous cloning studies in mouse CD8+ lymphocytes [15 ,18 ] showing that a few highly expressed microRNAs prevailed in frequency Interestingly, these microRNAs include miR16, ... suggested that both miR- 21 and miR -15 5 were more expressed in in vivo differentiated human primary CD8+ T cells than in their naive counterparts (Figure 2A) These results were confirmed by single ... microRNA belonging to the miR -17 ~92 cluster MiR- 21 has been shown to be upregulated in mouse CD8+ T cells upon in vitro activation [15 ], but its function in these cells remains unknown MiR -15 5,...
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báo cáo hóa học:" Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets" pptx
Ngày tải lên :
20/06/2014, 03:20
... is in agreement with previous cloning studies in mouse CD8+ lymphocytes [15 ,18 ] showing that a few highly expressed microRNAs prevailed in frequency Interestingly, these microRNAs include miR16, ... suggested that both miR- 21 and miR -15 5 were more expressed in in vivo differentiated human primary CD8+ T cells than in their naive counterparts (Figure 2A) These results were confirmed by single ... microRNA belonging to the miR -17 ~92 cluster MiR- 21 has been shown to be upregulated in mouse CD8+ T cells upon in vitro activation [15 ], but its function in these cells remains unknown MiR -15 5,...
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Báo cáo Y học: Regulation of RAS in human platelets Evidence that activation of RAS is not sufficient to lead to ERK1-2 phosphorylation pot
Ngày tải lên :
08/03/2014, 16:20
... and B) Human platelets were pretreated for 10 with 10 lM PP1 and stimulated for with UÆmL )1 thrombin, for with 10 lgÆmL )1 convulxin or for with 15 0 ngÆmL )1 TPO (A) Platelets were lysed by the addition ... platelets or [32P]orthophosphate-labelled platelets were pretreated for 10 with 10 lM Ro 31 8220 and stimulated for with UÆmL )1 thrombin, for with 10 lgÆmL )1 convulxin or for with 15 0 ngÆmL )1 TPO (A) ... thrombin induce the recruitment of GRB2 on phosphorylated adapters (A, B and C) Washed human platelets were stimulated with UÆmL )1 of thrombin for 12 0 s, 10 lgÆmL )1 convulxin for 12 0 s and 15 0...
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Báo cáo khoa học: Poly(ADP-ribose) polymerase-1 protects excessive DNA strand breaks from deterioration during repair in human cell extracts pot
Ngày tải lên :
23/03/2014, 13:20
... and that PARP -1 null cell lines display symptoms of genomic instability after treatment with DNA-damaging agents [16 18 ] Furthermore, the involvement of PARP -1 in BER is also supported by the ... poly(ADP-ribosyl)ated PARP -1 Therefore, the decreased binding of PARP -1 observed in Fig 2A may be interpreted as either PARP -1 dissociation or poly(ADP-ribosyl)ation However, crosslinking dynamics in the same ... 4A,B) Correspondingly, repair of the latter substrate involves more PARP -1 binding and more poly(ADP-ribosyl)ation, as observed in the formaldehyde cross-linking assay (compare Fig 4C,D) Therefore,...
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Báo cáo khoa học: The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines pdf
Ngày tải lên :
30/03/2014, 11:20
... for VRK3 [13 ] VRK1 has a nuclear localization signal and is detected in the nucleus in some cell lines and in transfected cells [11 ,13 ], but it is also present in the cytosol in other cell lines ... protein was more abundant in some cell lines, such as C4-I, HeLa, MCF7 or the colon carcinoma WiDr, and detected in smaller amounts in the remaining carcinoma cell lines (Fig 3A) In lymphoma cell ... of recombinant proteins J Virol 66, 4 413 –4426 11 Lopez-Borges S & Lazo PA (2000) The human vacciniarelated kinase (VRK1) phosphorylates threonine -18 within the mdm-2 binding site of the p53 tumour...
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báo cáo hóa học:" Preclinical evaluation of dasatinib, a potent Src kinase inhibitor, in melanoma cell lines" pptx
Ngày tải lên :
18/06/2014, 15:20
... added to the cells The plates were then stored at -20°C for hours After fixing the cells were stained according to the protocol for the TUNEL assay (Guava Technologies) Cells were analysed on the ... chemotherapy The effect of dasatinib in combination with chemotherapy was examined in the three dasatinib responsive cell lines, Lox-IMVI, HT144 and Malme-3M and in one of the dasatinib-resistant cell lines, ... The plates were then stored at 20°C for hours After fixing the cells were stained according to the protocol for the Guava Cell Cycle assay (Guava Technologies) Cells were analysed on the Guava...
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báo cáo hóa học:" Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF" ppt
Ngày tải lên :
18/06/2014, 15:20
... properties that depend on their origin within the wound Moreover, these cells appear to maintain a distinct phenotype in culture, suggesting that they can be used as a tool to test potential therapeutic ... sent for routine pathology and other sections were immediately processed for cell culture Another portion of these tissues were sent directly to the Aging Cell Repository at Coriell Institute for ... pM (matching probability) THO -1 D5S592 D10S526 vWA 31 D22S 417 FES/FPS 15 4 17 8 16 6–206 18 2–266 12 7 16 7 17 2– 213 206–234 0.77 0.83 0.84 0. 81 0.85 0.67 0.086; out of 12 0.0 51; out of 20 0. 017 ; out...
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Báo cáo sinh học: " Inhibition of phosphorylated c-Met in rhabdomyosarcoma cell lines by a small molecule inhibitor SU11274" pdf
Ngày tải lên :
18/06/2014, 19:20
... incubating them in serum-free medium for h followed by treatment with SU 112 74 (5 μM) or DMSO for 24 h Then the cells were either left untreated or they were treated with HGF (10 ng/ml) for 7.5 For the ... kinase-activating mutations in the c-Met receptor coding sequence [28,30-32] There were no activating mutations in the tyrosine kinase region of the c-Met receptor in the RMS cell lines used in our ... financial support, edit and finalize the manuscript All authors have read and approved the final manuscript 15 16 17 18 19 Competing interests The authors declare that they have no competing interests...
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Báo cáo sinh học: "Combination therapy with vemurafenib (PLX4032/RG7204) and metformin in melanoma cell lines with distinct driver mutations" pdf
Ngày tải lên :
18/06/2014, 19:20
... melanoma cells to each drug alone, both drugs were combined in constant ratios to each other Among the 11 BRAFV600E mutant cell lines, in cell lines the combination was antagonistic and in cell lines ... antagonistic effects with the combination of vemurafenib and metformin Page 11 of 13 The finding of some cell lines having divergent effects, together with the lack of predictive markers for either effect, ... note, the effects of single agent metformin or the combination with vemurafenib on the cell cycle were minimal in SKMEL173, the cell line that demonstrated enhanced proliferation with these treatments...
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Báo cáo hóa học: " Expression of frog virus 3 genes is impaired in mammalian cell lines" pot
Ngày tải lên :
20/06/2014, 01:20
... various time points post-infection, cells were fixed in 3.7% paraformaldehyde in phosphate buffer saline (PBS) for 10 minutes, and permeabilized in a 0 .1% Triton X -10 0 solution for minutes Indirect ... expressed in mammalian cell lines However, essential viral genes must express in mammalian cell lines since the virus is able to infect and successfully replicate in many cell lines, including rodent, ... FHM cells, an ectothermic cell line Although we demonstrated that FV3 can infect BGMK cells, we wanted to know whether FV3 produced infectious virions BGMK cells were either mock infected or infected...
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báo cáo hóa học:" Inhibition of phosphorylated c-Met in rhabdomyosarcoma cell lines by a small molecule inhibitor SU11274" pdf
Ngày tải lên :
20/06/2014, 03:20
... incubating them in serum-free medium for h followed by treatment with SU 112 74 (5 μM) or DMSO for 24 h Then the cells were either left untreated or they were treated with HGF (10 ng/ml) for 7.5 For the ... kinase-activating mutations in the c-Met receptor coding sequence [28,30-32] There were no activating mutations in the tyrosine kinase region of the c-Met receptor in the RMS cell lines used in our ... financial support, edit and finalize the manuscript All authors have read and approved the final manuscript 15 16 17 18 19 Competing interests The authors declare that they have no competing interests...
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Báo cáo y học: " DNA methylation patterns associate with genetic and gene expression variation in HapMap cell lines" ppt
Ngày tải lên :
09/08/2014, 22:23
... Biology 2 011 , 12 :R10 http://genomebiology.com/2 011 /12 /1/ R10 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Ferrucci L, Longo DL, Cookson MR, Singleton AB: Abundant quantitative trait Loci exist for DNA ... transformation of peripheral blood mononuclear Bell et al Genome Biology 2 011 , 12 :R10 http://genomebiology.com/2 011 /12 /1/ R10 cells using phytohemagluttinin We obtained the transformed cell lines ... present only in the original data, and 24 new eQTLs were present only in the methylation-residuals (Figure 4b) Interestingly, the SNPs that were eQTLs for the 45 genes with reduced signals in the methylation-residuals...
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Báo cáo y học: "LMP-420, a small-molecule inhibitor of TNF-alpha, reduces replication of HIV-1 and Mycobacterium tuberculosis in human cell" pps
Ngày tải lên :
10/08/2014, 05:20
... ± 80 ± 2,8 61 ± 98 93 ± 2, 714 ± 13 2 19 ,554 ± 63 424 ± 7,628 ± 11 2 10 ,670 ± 2 71 18 ± 419 ± 13 3 1 17,249 ± 10 3 24 ± 17 ,575 ± 262 1, 766 ± 62 76 ± 33 ± 56 ± 4,794 ± 803 63 ± 14 3 ± 19 ,12 7 ± 520 262 ... IL -1 IL-2 IL-4 IL-5 IL-6 IL-7 IL-8 IL -10 IL -12 IL -13 IL -17 G-CSF GM-CSF MCP -1 MIP -1 IFN-γ TNF-α + LMP-420 (1 µM) 15 , 829 ± 75 24 ± 582 ± 54 7±3 16 , 512 ± 19 0 26 ± 17 ,16 0 ± 2 21 1,963 ± 17 51 ± ... LMP-420 and /or zidovudine (AZT) on HIV- 1 repliEffects of LMP-420 and /or zidovudine (AZT) on HIV- 1 replication PBMC (10 6/ml) were infected with HIV- 1Ba-L for h, treated with LMP-420 and /or AZT at...
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