... described that involve dsRNA formation dsRNA formation can interfere with biological activities that require binding of RNA or proteins to the transcript [13] This may include processes such as RNA ... An unwinding activity that covalently modifies its double- strandedRNA substrate Cell 1988, 55:1089-1098 Zhang Z, Carmichael GG: The fate of dsRNA in the nucleus: a p54nrb-containing complex mediates ... translation Alternatively, dsRNA could function as a trigger for an RNAi process, providing a target for Dicer enzymes, and be specifically degraded to small interfering RNA (siRNA) molecules...
... double- stranded RNA- binding domain of Rnt1p selectively binds RNA containing a 5¢-AGNN-3¢ tetraloop RNA 6, 1142–1156 Doyle M & Jantsch MF (2003) New and old roles of the double- stranded RNA- binding ... The doublestranded RNA- binding protein Xlrbpa promotes RNA strand annealing Nucleic Acids Res 26, 4382–4388 Zheng X & Bevilacqua PC (2000) Straightening of bulged RNA by the double- stranded RNA- binding ... binding to double- strandedRNA J Virol 70, 2611–2614 Castrignano T, Chillemi G, Varani G & Desideri A (2002) Molecular dynamics simulation of the RNA complex of a doublestranded RNA- binding...
... human double- strandedRNA activated protein kinase induced by interferon Cell 62, 379–390 Hovanessian, A.G (1989) The doublestranded RNA- activated protein kinase induced by interferon: dsRNA-PK ... of double- stranded RNA- activated protein kinase (PKR) in the cell death by influenza virus infection J Virol 70, 8128–8132 21 Takizawa, T., Tatematsu, C & Nakanishi, Y (1999) Doublestranded RNA- activated ... protein kinase and c-Jun NH2-terminal kinase by double- strandedRNA and encephalomyocarditis virus: involvement of RNase L, protein kinase R., and alternative pathways Mol Cell Biol 20, 617–627 28...
... G (2000) RNA recognition by a Staufen double- stranded RNAbinding domain EMBO J 19, 997–1009 45 St Johnston, D., Brown, N.H., Gall, J.G & Jantsch, M (1992) A conserved double- stranded RNA- binding ... Ryter, J.M & Schultz, S.C (1998) Molecular basis of doublestranded RNA protein interactions: structure of a dsRNA-binding domain complexed with dsRNA EMBO J 17, 7505–7513 43 Nanduri, S., Carpick, ... similarity to the double- stranded RNA- binding domain and (b) proteins with similarity to the C-terminal domain of glycyl-tRNA synthetase The first group includes exclusively RNA- binding proteins...
... interference by double- strandedRNA in Caenorhabditis elegans Nature 391, 806–811 30 Dzitoyeva S, Dimitrijevic N & Manev H (2001) Intra-abdominal injection of double- strandedRNA into Functional ... using double- strandedRNA To investigate the ability of dsRNA to disrupt the CHH function in adult shrimps, cDNA corresponding to the CHH mature peptide was used as template for synthesizing dsRNA ... corroborate the specificity of the dsRNA gene silencing mechanism, we included a group of shrimps that were injected with 20 lg of dsRNA unrelated to CHH mRNA The unrelated dsRNA was generated from L schmitti...
... incubated with a reaction mixture containing [32P]ATP[cP] FEBS Journal 272 (2005) 1568–1576 ª 2005 FEBS Control of protein kinase R degradation Fig Double- stranded RNA- dependent protein kinase (PKR) ... Role of double- stranded RNA- dependent protein kinase in mediating hypersensitivity of Fanconi anemia complementation group C cells to interferon gamma, tumor necrosis factor-alpha, and doublestranded ... IFN-inducible dsRNA-dependent protein kinase Science 257, 1685–1689 Meurs EF, Galabru J, Barber GN, Katze MG & Hovanessian AG (1993) Tumor suppressor function of the interferon-induced double- stranded RNA- activated...
... to isolate dsRNA of Uncinula necator, requires that the dsRNA samples be treated with RNase A to eliminate ssRNAs that are visualized as smears in the lanes where the obtained dsRNA samples had ... help to eliminate capsid proteins We think that this method may be used to isolate dsRNA from any biological sample that contains dsRNA and that the dsRNA obtained can be used for further downstream ... was required We consider that this methodology may be used to isolate dsRNA from any type of fungi and also can be applied to isolate genomic RNA from dsRNA virusesthat infect cells of higher...
... such that [RNA] t = [RNA] (t-1)ek, where [RNA] t is virus concentration at time (t) and k a growth constant However, because of RNApol infidelity, wild-type (wt) virus will accumulate at [RNAwt]t ... ratio of ([RNAwt](t-1)•ρ + [RNAmt](t-1))/ [RNAwt](t-1)•(1-ρ) compared to wild type) and variant viral RNAs will rapidly predominate (Figure 1) Mutations progressively accumulate in RNAviruses [17] ... requires: i) that viral envelope (Env) proteins interact with viral RNA polymerases (RNAPol); ii) that these Env :RNAPol interactions alter both polymerase processivity and fidelity; iii) that wild-type...
... denaturation of the double- stranded DNA (B) 5344 formation of these structures may be used for the purpose of grafting ligands to double- stranded DNA [12] or for visualizing short double- stranded sequences ... complexes where a TFO encircles its double- stranded DNA target Our previous attempts came up with the fact that formation of the triple helix requires a pH that is too low to enable efficient ligation ... stem–loop oligonucleotide encircles a double- stranded DNA molecule Formation of these structures uses triple-helix formation to wind an oligonucleotide around a double- stranded DNA target The TFO is...
... single -stranded nick into the active origins generating two types of replicative form DNA: an extended palindromic form, and a turnaround form that recreates the left-hand termini [3,14,18] The turnaround ... the GA doublet is unimportant, but insertion of any third nucleotide here inactivates the origin, suggesting that it represents a critical spacer element [5] The junction region thus formed contains ... the mismatched doublet GA and triplet GAA are now based paired to their complementary sequences The sequence surrounding the doublet is a potent origin, but the analogous region containing the...
... single -stranded nick into the active origins generating two types of replicative form DNA: an extended palindromic form, and a turnaround form that recreates the left-hand termini [3,14,18] The turnaround ... the GA doublet is unimportant, but insertion of any third nucleotide here inactivates the origin, suggesting that it represents a critical spacer element [5] The junction region thus formed contains ... the mismatched doublet GA and triplet GAA are now based paired to their complementary sequences The sequence surrounding the doublet is a potent origin, but the analogous region containing the...
... erythematosus [14], there is little evidence that anti-dsDNA antibodies are pathophysiologically involved in other clinical manifestations Recent findings that intrathecal binding of anti-dsDNA antibodies ... be confirmed in patient cohorts [15] Assuming that antibodies detected in serum truthfully reflect the process in situ, we therefore need assays that can measure pathogenicity, avidity or specificity ... site and stage of anti-DNA B-cell deletion Nature 1995, 373:252-255 Rekvig OP, Nossent JC: Anti -double- stranded DNA antibodies, nucleosomes, and systemic lupus erythematosus: a time for new paradigms?...
... factor family members [3,7] The inducible co-stimulator (ICOS; also known as AILIM [activation-inducible lymphocyte immunomediatory molecule]) was identified in 1999 as a membrane glycoprotein that ... its importance as a therapeutic target We found that ICOS was over-expressed in peripheral blood CD4+ T cells from patients with active SLE and that ICOS contributed not only to the enhanced proliferation ... ability of peripheral blood T cells from patients with SLE to support the production of IgG anti -double stranded (ds)DNA antibody by autologous peripheral blood B cells Thus, we examined the expression...
... modified with a fluorophore (Dye633) at their amino-termini to allow quantitative detection The double- stranded DNA targets were made of custom-made complementary oligonucleotides carrying the appropriate ... fibre-opticaldevice developed in our institute (Figure 2), which was driven by the in-house built software The doublestranded DNA targets were immobilized on a silanized glass fibre and incubated with the zinc ... work we have shown that synthetic zinc fingers bind specifically to their binding sites which consist of nucleic acid triplets An additional advantage of the SP1 system is that it offers an opportunity...
... procedure and was used as an internal amplification control by Stocher and Berg [4,20] In this study, we constructed a double- stranded DNA control or standard that contained a 1,349-bp HBV fragment ... structure of the target nucleic acids [25] Obviously, single -stranded DNA controls that are derived from filamentous phage cannot mimic HBV double- stranded DNA properties such as charge and secondary ... phage packaging in vitro http://www.virologyj.com/content/6/1/226 Lambda phage is a double- stranded DNA phage that can package exogenous DNA into phage particles in vitro The constructed phage...
... preparation does not contain steroid hormones and RNA It gives negative PCR results for hepatitis B virus DNA, hepatitis C virus RNA, HIV DNA, HIV RNA The DNA preparation does not contain histones ... inducers of antitumor immunity has been intense over the past years It has been revealed that mammalian double- stranded DNA (dsDNA) induces both humoral and adaptive immune responses [15,22,23] This ... Chernykh ER, Zagrebelniy SN, Bogachev SS, Shurdov MA: Exogenous allogenic fragmented double- stranded DNA is internalized into human dendritic cells and enhances their allostimulatory activity Cell...
... Vaccines and Therapy 2011, 9:4 http://www.gvt-journal.com/content/9/1/4 The genome of single stranded AAV vector has to be converted to double- stranded replicative form once it has entered the ... titers of single -stranded AAV (ssAAV) serotypes 1,2,4,5 and AAV2 still had the highest efficiency compared to the other serotypes Thus, we hypothesized that a self-complementary double- stranded AAV2 ... of AAV [42,43] Double- stranded, self-complementary AAV vectors bypass this step and provide the opportunity to achieve more efficient transduction [44] McMahon et al [45] reported that rat bone...
... of this family are also known as herpesviruses Herpesviruses share a common structureall herpesviruses are enveloped, double- stranded DNA viruses with relatively large complex genomes that range ... of the DNA double helix can be pulled apart easily due to high AT content (deHaseth et al., 1995) 1.2 Herpesviruses Herpesviridae is a large family of linear, double- stranded DNA viruses with ... approach working on subfamily and all genome sequences of herpesviruses 103 Chapter1: Introduction Chapter Introduction Herpesviridae is a large, ancient family of DNA virusesthat infect many vertebrates...
... Planning Process Optimization Measurement Integration @heinzmarketing #mktgnation Page Planning: “No, that is NOT a campaign!” Planning Best Practices Campaigns vs programs vs “sends” Know the objective...