vascular regeneration endothelial progenitor cell therapy for ischemic diseases
Báo cáo y học: " Cell therapy for autoimmune diseases" ppt
... discuss the current and future prospects for such cell- based therapies Hematopoietic stem cell transplantation for autoimmune diseases Hematopoietic stem cell transplantation (HSCT) is a treatment ... technologies for cell purification and protocols for expanding specific subsets are more advanced, there are opportunities for achieving immune homeostasis by infusion of regulatory cell populations, ... the thymus While progenitor T cells with no affinity or high affinity for self-peptide–MHC ligands die, those with a low affinity survive Potentially autoreactive T cells therefore persist after...
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báo cáo hóa học:" Non-expanded adipose stromal vascular fraction cell therapy for multiple sclerosis" pptx
... SVF cells isolated from subcutaneous or visceral adipose tissue contain a population of cells positive for CD34, CD133 and the drug efflux pump ABCG2 [30] These cells had endothelial colony forming ... selection for certain cell populations such as MSC and decreases the number of CD34 cells [38] Thus in vitro expanded SVF derived cells can not be compared with primary isolated SVF cells Immune ... adipose tissue-derived stem cells Circulation 2004, 110:349-355 Urbich C, Dimmeler S: Risk factors for coronary artery disease, circulating endothelial progenitor cells, and the role of HMG-CoA...
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Báo cáo y học: "tromal Vascular Fraction Transplantation as an Alternative Therapy for Ischemic Heart Failure: Anti-inflammatory Role" doc
... adipose tissue are multipotent cells that can differentiate into cardiomyocytes [9,10] and vascular endothelial cells [11,12] Therefore, adipose-derived stromal vascular fraction (SVF) emerging ... Page of 10 blood cells Cells were labeled with a fluorescent dye using PKH26 (PKH26 Red Fluorescent Cell Linker Mini Kit, for General Cell Membrane Labeling, SIGMAALDRICH Inc.) [15] Cells were suspended ... group Cell transplants PKH26 labelled transplanted cells were detected in host myocardium by their intense red fluorescence, week after cell implantation (Figure 1) Effects of cell therapy on vascular...
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Báo cáo y học: "Endothelial progenitor cell biology in disease and tissue regeneration" doc
... venules [57] Hemangioblasts, Endothelial Progenitor Cells, Vascular Endothelial Cells [10] VEGFR-1 (Flt1) Tyrosine kinase receptor for VEGF A and B, important for endothelial cell assembly into vessels ... precursor cells that aid in neovascularization are known as endothelial progenitor cells EPCs: Physiological and Biological Functions Endothelial progenitor cells (EPCs) are bone marrow derived cells ... pro-migration ability [59] Hemangioblasts, Endothelial Progenitor Cells, MAPC, Myelo/Monocytic Cells, Vascular Endothelial Cells, Lymphatic Endothelial Cells [10] CD133 (Prominin 1) Membrane glycoprotein,...
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Báo cáo hóa học: "Safety evaluation of allogeneic umbilical cord blood mononuclear cell therapy for degenerative conditions" pot
... control: Tests (HBV, HCV, HIV, ALT and Syphilis) for communicable diseases for UCB units are carried out before any processing begins Testing was performed by third party laboratory under local government-monitored ... blood cells for regenerative medicine applications in absence of immune suppression would overcome several substantial hurdles existing today in stem cell therapy Although cord blood derived cells ... treatment Helper T -cell (Th cell/ CD4) % of total T cells 114 48.84 (9.03) 45.44 (10.65) 28-58
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o cáo hóa học:" Summit on cell therapy for cancer: The importance of the interaction of multiple disciplines to advance clinical therapy" ppt
... × 109 cell used for adoptive cytotoxic T cell and NK cell therapy Several groups are currently working to develop and validate methods to expand, TIL, T cells, engineered T cells and NK cell in ... Summit on Cell Therapy for Cancer The meeting will include lectures on adoptive cellular therapy using tumor infiltrating lymphocytes (TIL), cytotoxic T cells and natural killer (NK) cells, reprogramming ... flasks specifically designed for good manufacturing practice (GMP) cell growth that will further simply TIL, T cell and NK cell production Adoptive cell therapy requires cell growth and culture in...
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Báo cáo y học: "Dendritic cell therapy for oncology roundtable conference Sandra Tuyaerts" doc
... should interact with T cells Jeffrey Weber therefore designed a system to attract T cells to the injection site and induce in this way and “artificial lymph nodal aggregate” for T cell priming To achieve ... immunerelated response pattern (as described for anti-CTLA4 therapy) Therefore, immune-related response criteria (irRC) may be more relevant than RECIST/WHO criteria for the assessment of anti-tumor activity ... responses, and nearly three fourths had T cell proliferative responses Survival was improved for patients who had an antibody response but not for those with a T cell response Although these data are...
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Báo cáo y học: "Enhanced late-outgrowth circulating endothelial progenitor cell levels in rheumatoid arthritis and correlation with disease activity" doc
... Figure Positive correlation between endothelial progenitor cell (EPC) counts and circulating endothelial cell (CEC) counts (cells per 106 lineage-negative mononuclear cells) (a) Patients with rheumatoid ... 7-aminoactinomycin D; VEGFR-2, vascular endothelial growth factor receptor-2 Endothelial colony formation has previously been used as an alternative method to detect endothelial progenitors in PBMCs [20] ... Arthritis Research & Therapy 2010, 12:R27 http://arthritis-research.com/content/12/1/R27 Page of Â Ê Â Ă Figure Endothelial progenitor cell (EPC) and circulating endothelial cell (CEC) levels...
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Báo cáo y học: "15-deoxy-delta12,14-prostaglandin J2 attenuates endothelial-monocyte interaction: implication for inflammatory diseases" ppsx
... expression of endothelial CAMs bEND.3 cells were pretreated with 15d-PGJ2 (5–20 μM) for 30 followed by stimulation with TNFα (50 ng/ml) for h Cells were harvested in Trizol reagent for RNA isolation ... adhesion of mononuclear cells to the endothelial cells and thereby attenuates their transmigration We observed that 15dPGJ2 inhibited the adhesion of monocytes to bEND.3 endothelial cell line, activated ... BEND.3 cells were grown in chamber slides and treated with 15d-PGJ2 and stimulated with TNFα for 20 Cells were fixed with paraformaldehyde (4%) followed by blocking in blocking reagent Cells were...
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Báo cáo sinh học: "AAV-mediated gene therapy for metabolic diseases: dosage and reapplication studies in the molybdenum cofactor deficiency model" pps
... with an exposure time of 50 ms for DAPI and 500 ms for EGFP (1 s for negative expression) Page of (page number not for citation purposes) Genetic Vaccines and Therapy 2009, 7:9 http://www.gvt-journal.com/content/7/1/9 ... borderline result and indicates a range for the minimal dosage required for abolishing the MoCo deficiency phenotype With a maximum body weight of 40 g for the mice used here this would correspond ... perfused with 4% paraformaldehyde Tissue for expression analysis was cryoprotected in sucrose and stored frozen at -80° until analysis Cryostat sections of μm thickness were prepared for EGFP expression...
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Báo cáo y học: "Comparison between single antiplatelet therapy and combination of antiplatelet and anticoagulation therapy for secondary prevention in ischemic stroke patients with antiphospholipid syndrome"
... Med Sci 2010, with APS We therefore compared single antiplatelet therapy and a combination of antiplatelet and anticoagulation therapy for secondary prevention in ischemic stroke patients with ... (APASS) [3] for prevention of stroke in patients with ischemic stroke or transient ischemic attack and with antiphospholipid antibodies (aPL), antiplatelet therapy is reasonable for cases of ... ischemic stroke Annual Review 2004; 1: 139-51 Sacco RL, Adams R, Albers G, et al Guideline for prevention of stroke in patients with ischemic stroke or transient ischemic attack A statement for...
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báo cáo hóa học:" Identifying alemtuzumab as an anti-myeloid cell antiangiogenic therapy for the treatment of ovarian cancer" pdf
... myeloid cells remains controversial, murine studies confirm a critical role for these cells in tumorigenesis and indicate that these cells may be novel therapeutic targets for solid tumor therapy ... serum Cells negative for both Annexin V and PI were deemed viable cells Complement-mediated Cytotoxicity of Whole Ascites A single cell suspension of whole ascites cells (host and tumor cells) ... Reverse-transcription polymerase chain reaction; T regs: Regulatory T cells; VE-Cadherin: Vascular Endothelial Cadherin; VLC: Vascular leukocytes; VEGF: Vascular Endothelial Growth Factor Competing interests The...
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Báo cáo y học: " Chitosan Interferon-c Nanogene Therapy for Lung Disease: Modulation of T-Cell and Dendritic Cell Immune Responses" pps
... through a cell strainer to produce single -cell suspensions The cells were pelleted by centrifugation at 500g for minutes at 4uC, and then red blood cells were lysed by resuspending the cell pellets ... nodes through 40-micron cell strainers, and CD11c+ cells were isolated by magnetic bead separation The cells were stained for CD11b and separately for each of the dendritic cell activation markers ... single -cell suspensions of T cells The OVA-specific CD8+ T -cell population was labelled with phycoerythrin-tagged tetramer for OVA peptide conjugated with MHC-I CD8+ cells were analyzed for apoptosis...
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Báo cáo khoa học: " Stereotactic body radiation therapy for melanoma and renal cell carcinoma: impact of single fraction equivalent dose on local control" ppt
... posttreatment PET scan were included for analysis The PET scans were performed intermittently for tumor surveillance and regularly in patients undergoing chemotherapy for other sites of disease The ... survivors and chance for cure with a year OS rate of 39% [30] The argument for using ablative local therapy for isolated metastases is strengthened if effective systemic therapy is available ... radiotherapy (SBRT) for stage I non-small -cell lung cancer Lung Cancer 2007, 56:229-234 doi:10.1186/1748-717X-6-34 Cite this article as: Stinauer et al.: Stereotactic body radiation therapy for...
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Báo cáo khoa học: " Absence of toxicity with hypofractionated 3-dimensional radiation therapy for inoperable, early stage non-small cell lung cancer" pot
... Radiation therapy alone in early stage non-small cell lung cancer Semin Surg Oncol 2003, 21:91-97 Sibley GS: Radiation therapy for patients with medically inoperable Stage I non small cell lung ... ordered only for patients with a suspicious sign or symptom of distant metastases The optimal accelerated regimen of conformal 3-dimensional hypofractionated radiation therapy (3DHRT) for this group ... co-morbid diseases Co-morbid conditions have been shown to affect prognosis in early stage NSCLC [8] Unfortunately, the typical early stage NSCLC patients referred for curative radiation therapy...
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báo cáo khoa học: "Evolution of T-cell clonality in a patient with Ph-negative acute lymphocytic leukemia occurring after interferon and imatinib therapy for Ph-positive chronic myeloid leukemia" pot
... expansion of host T-cells or the allogeneic T-cells These activated T-cells have been shown to display potential cytotoxic activity against primary leukemic cells Thus, it may be useful for eradication ... leading to clonal T -cell selection in CML are unknown, the change of TCR Vb clones might provide the information for host immune response After two courses of chemotherapy for Phnegative ALL, ... at 94°C for with 9.5 μl formamide (Hi-Di Formamide, ABI, USA) and 0.5 μl of Size Standards (GENESCAN™-500-LIZ™, Perkin Elmer, ABI), the samples were then loaded on 3100 POP-4™ gel (Performance...
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Báo cáo y học: "Squamous cell carcinoma of the nipple following radiation therapy for ductal carcinoma in situ: a case report" ppt
... significant for right breast DCIS for which she had undergone lumpectomy and whole breast radiation therapy in February 1998 These interventions were followed by five years of tamoxifen therapy By ... radiation therapy) Radiation therapy has also been described as a risk factor for SCC In a report from the Childhood Cancer Sur- Figure p63 immunostaining of SCC Page of vivor study, radiation therapy ... the nipple following radiation therapy for breast carcinoma were found This result is surprising given the large number of patients undergoing radiation therapy for breast cancer Overall, SCC...
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Báo cáo y học: " Effect of erythropoietin on level of circulating endothelial progenitor cells and outcome in patients after acute ischemic stroke" ppsx
... mobilization of bone marrow-derived endothelial progenitor cells for neovascularization Nat Med 1999, 5:434-438 34 Luttun A, Carmeliet G, Carmeliet P: Vascular progenitors: from biology to treatment ... without EPO treatment EPC = endothelial progenitor cell; EPO = erythropoietin; RBC = red blood cell; WBC = white blood cell Yip et al Critical Care 2011, 15:R40 http://ccforum.com/content/15/1/R40 ... Magner M, Isner JM: Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization Circ Res 1999, 85:221-228...
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Báo cáo sinh học: "Silencing the epidermal growth factor receptor gene with RNAi may be developed as a potential therapy for non small cell lung cancer" pot
... per well Forty-eight hours after transfection, cell numbers were determined using a hemocytometer For colony forming assay, cells transfected with siRNA, Page of 12 (page number not for citation ... 20 µl (1 mg/ ml) was added to each well for h at 37°C to allow MTT to form formazan crystals by reacting with metabolically active cells Subsequently the formazan crystals were solubilized by 150 ... EGFR expression, we performed two experiments; one was a cell count assay and another, a colony forming assay Cell count results showed a significant decrease in the number of cells by 85.0% in A549...
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ENGINEERING THREE DIMENSIONAL CULTURE PLATFORMS FOR HUMAN ADIPOSE DERIVED STEM CELL THERAPY
... systems for stem cell therapy 20 2.4.1 Effect of mechanical cues on stem cells 21 2.4.2 Effect of biomolecular cues on stem cells 22 2.4.3 Hydrogels for stem cell therapy 23 2.4.4 Microspheres for ... cell delivery strategy for adipose derived stem cell therapy 1.2 Hypothesis The three dimensional cell- microsphere (ADSC-GMs) constructs formed using ADSCs and GMs with strong cell- cell and cell- matrix ... allow cell culture on their surfaces and forms cell- microsphere contructs with strong cell- cell and cell- material interactions which are crucial for tissue regeneration Reproduced from (Hernandez...
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