vascular endothelial growth factor vegf and its role in non endothelial cells autocrine signalling by vegf
Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... [8] and Ras guanine exchange factor (RasGEF) veryKIND (v-KIND, or kinase noncatalytic C-lobe domain containing 1) [9] The KIND domain in these proteins is localized to the N-terminal region, and ... very-KIND-MAP2 interaction v-KIND KIND2 KIND2-1 KIND2-2 KIND2-3 KIND2-4 KIND2-5 A 620 521 555 488 589 EGFP EGFP-KIND2-1 EGFP-KIND2-2 EGFP-KIND2-3 EGFP-KIND2-4 EGFP-KIND2-5 EGFP-KIND2 EGFP-KIND1 ... v-KIND-MAP2 interaction within cells The MAP2-binding core module of v-KIND is involved in targeting to neuronal dendrites and dendrite growth We next investigated the MAP2 binding core of v-KIND...
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Báo cáo y học: "Downregulation of protein disulfide isomerase in sepsis and its role in tumor necrosis factor-alpha release" pps
... bacitracin, a PDI inhibitor Finally, PDI expression was induced by the antiinflammatory cytokine IL-10, and IL-10-mediated inhibition of LPS-induced IL-6 expression was reduced by bacitracin These findings ... molecular chaperones in the folding of oxidized proteins Refolding of colloidal thyroglobulin by protein disulfide isomerase and immunoglobulin heavy chain-binding protein J Biol Chem 2001, 276:21337-21342 ... Effect of protein disulfide isomerase inhibition by short interfering RNA on tumor necrosis factor- alpha gene expression and release in RAW 264.7 cells To further confirm the role of PDI in the regulation...
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Tài liệu Báo cáo khoa học: A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and invasion docx
... exon and exon 1a, we compared the promoters involved in Chang and Huh7 cells As shown in Fig 1D, PCR products containing exon were produced in Huh7 cells but not in Chang cells To determine whether ... Chang cells Furthermore, in a comparative analysis between OccWT and OccDE9, the exon region played a significant role in promoting apoptosis and inhibiting invasion by regulating signaling pathways ... localized in a more disconnected fashion than that in Hep3B cells In Chang cells, stained occludin was observed in the cytoplasmic region Additionally, in OccWT-overexpressing Hep3B and Huh7 cells, ...
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... copper binding could still allow domain swapping to occur It is of interest that a N-terminally truncated prion protein, lacking the copper binding domain is capable of domain swapping and forms ... binding on the secondary structure was determined using CD in the far UV To prepare proteins without Cu2+, part of each protein 4252 Fig Comparison of copper binding by stefin A and stefin B using ... C-terminus (box in Fig 1) at sites: 92, 75, 66 and 58 As histidine residues are central to copper binding in many proteins they probably form part of the copper binding sites in this protein Although...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... in the AUH gene This indicated that AUH, in addition to its RNA binding function, must play an important role in leucine catabolism AUH was overproduced in E coli and characterized by measuring ... of leucine catabolism at the protein and DNA levels and developed a novel assay for enzyme analysis in a diagnostic setting The human AUH protein was first recognized by its ability to bind A + ... (c.719C>T) in exon and an insertion mutation c.613–614insA This insertion causes a frameshift that starts at Met205 and leads to the introduction of a stop codon after four amino acids The intra-assay...
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Tài liệu Báo cáo khoa học: Inorganic pyrophosphatase in the roundworm Ascaris and its role in the development and molting process of the larval stage parasites doc
... (Michaelis constant) and Vmax (maximum velocity) values were determined by incubating the diluted recombinant proteins in the standard reaction mixture in the presence of increasing concentrations ... were removed and minced with a surgical knife The minced tissue was wrapped in cotton gauze and suspended in NaCl/Pi containing 100 lgÆmL)1 penicillin/ streptomycin at 37 °C for h After incubation ... were incubated at 37 °C in a humidified 5% CO2 incubator in the absence (control) and presence of increasing concentrations of inhibitors for 10 days, and the number of molting larvae was determined...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
... functions including apoptosis in infected and uninfected bystander target cells through its interaction with host cellular proteins [22–25] However, it is not clear at this point how interaction ... (RTK) signaling, independent of MAP kinase (MAPK) by either functioning downstream of MAPK or by defining a new pathway of RTK signaling [8] RTKs play important roles in cell signaling during cell ... method PBMC and PBL were blasted with PHA-P (5 lgÆmL)1, Sigma, St Louis, MO) for days and cultured in RPMI containing 10% FBS, 1% penicillin– streptomycin and interleukin-2 growth factor Monocytes...
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Solar and Space Physics and Its Role in Space Exploration doc
... Printing Office, Washington, D.C., 2004 SOLAR AND SPACE PHYSICS AND ITS ROLE IN SPACE EXPLORATION the fundamental role of solar and space physics research both in scientific exploration and in ... affected by magnetic shielding produced by CMEs as they propagate through interplanetary space Understanding these interactions requires understanding first the ambient background by drawing, in part, ... Engineering was established in 1964, under the charter of the National Academy of Sciences, as a parallel organization of outstanding engineers It is autonomous in its administration and in the...
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Báo cáo khoa học: Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins pptx
... approximately 0.5 to induce the expression of the protein and growth was continued for h Cells were collected by centrifugation (3000 g, 10 min, °C) and resuspended in cell lysis buffer containing 50 mM ... no membrane-spanning domains in PEX14 have been unambiguously identified and it was observed in mammalian cells and S cerevisiae that both the N-terminal and the C-terminal domain are at the cytosolic ... Gel-retardation assay of TbPEX5 and TbPEX14 interaction, under nondenaturing and nonreducing conditions (A) Purified His6tagged TbPEX5 and a protein comprising the N-terminal part of TbPEX14 (TbPEX14-N,H)...
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Báo cáo y học: "ignal 3 and its role in autoimmunity" pot
... online http://arthritis-research.com/content/6/1/26 known roles in tissue inflammation, and damage in innate immunity Besides its capacity to drive the production of IFN-γ and IL-2 by CD4+ T cells ... tolerance in the thymus Competing interests None declared References 5.* 10 11 12 13.* 14 15 16 Delon J, Stoll S, Germain RN: Imaging of T-cell interactions with antigen presenting cells in culture and ... highlights the role of innate and adaptive immune system interactions in the failure of peripheral tolerance It will be fascinating in the future to extend this concept to understand the impact...
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Báo cáo y học: "Activation of transforming growth factor-β1 and early atherosclerosis in systemic lupus erythematosus" pptx
... For annexin-V/PI staining × 106 cells were resuspended in 50 µl binding buffer (Roche Diagnostics, Lewes, UK) and incubated with annexin-V/FITC (Roche) and annexin-V/PI (Roche) for 15 minutes at ... statins and aspirin? Best Pract Res Clin Rheumatol 2005, 19:823-838 27 Grainger DJ, Byrne CD, Witchell CM, Metcalfe JC: Transforming growth factor beta is sequestered into an inactive pool by ... labelled cells and the percentages of apoptotic (fluorescein isothiocyanate [FITC]+PI-) and necrotic (FITC+PI+) cells determined Cells in later stages of apoptosis were analyzed using PI staining...
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Báo cáo y học: "Circadian rhythm and its role in malignancy" docx
... loss during intestinal tumorigenesis may, in part, act through upregulation of b-catenin, increasing intestinal b-catenin signaling and cell proliferation Also, increase in small-intestinal mucosa ... regulating the circadian clock include acetylation, phosphorylation, ubiquitination and sumoylation In terms of phosphorylation, Casein kinase epsilon (CK1ε) and Casein kinase delta (CK1ε and CK1δ), ... damage-activated checkpoint proteins Per1 overexpression in cancer cells increases ionizing radiationinduced apoptosis, whereas inhibition of Per1 in similarly treated cells blunts apoptosis Ionizing radiation...
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Báo cáo y học: " Beyond the first 25 years: The International AIDS Society and its role in the global response to AIDS" pps
... also increasingly involved in the development and delivery of a range of education and training programmes designed to help individuals working in HIV advance professionally, from administering ... Providing education and training opportunities for its membership is important for any professional society, and the IAS is looking at ways to strategically expand its role in this area in order ... the coming decades The IAS has a unique opportunity – and responsibility – to bring together individuals working in diverse settings and disciplines, from areas of the world separated by geography,...
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MECHANISM OF TISSUE TRANSGLUTAMINASE UPREGULATION AND ITS ROLE IN OVARIAN CANCER METASTASIS
... are involved both in GDP and ATP binding to TG2 The residue Arg580, which is important for GTP/GDP binding, is not involved in ATP binding (Han et al 2010) By purifying the insulin-like growth factor- binding ... at its protease sensitive hinge region, which is between the N-terminal ECM binding domain and the C-terminal LAP binding domain This can be achieved by many proteinases in the serine proteinase ... invasiveness in vitro and tumor metastasis in vivo The N-terminal fibronectin (FN) binding domain of TG2 (tTG 1-140), lacking both enzymatic and GTPase function, induced EMT in OC cells, suggesting the interaction...
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THE MECHANISMS REGULATING THE TRANSCRIPTION FACTOR ATF5 AND ITS FUNCTION IN THE INTEGRATED STRESS RESPONSE
... heme-binding sites, one in the N-terminus, and a second located in an insert region in the middle of the protein kinase domain (Fig 3) Heme binding inhibits HRI kinase activity, and in response to heme ... chaperone whose expression is induced by UPR during ER stress (25-27) GRP78/Bip has an ATPase domain in its N-terminus, and a peptide binding domain in its C-terminus GRP78 binds to the hydrophobic ... Wenzhao, Jingliang Yan, Lin Lin and Sixin Jiang I would like to thank my parents, Xin Zhou and Shulan Yang, and my motherand father -in- law, Xihong Mo and Bin Zhong, for making the life of my wife and...
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REGULATION OF CHOP TRANSLATION IN RESPONSE TO eIF2 PHOSPHORYLATION AND ITS ROLE IN CELL FATE
... expressed predominantly in erythroid cells HRI is regulated by heme through the two heme-binding regions in HRI: an N-terminal domain of HRI and in an insert region in the protein kinase domain of HRI ... affect insulin folding process (144) As a consequence, the unfolded insulin forms aggregates in a dominant fashion, rendering insulin non- functional (145, 146) The aggregated mutant insulin proteins ... splicing of mRNA encoding the Xbox binding protein1 (XBP1) in mammals and homologue of the mammalian activating transcription factor/ cAMP-response element (CRE)-binding protein (HAC1) in yeast (102,...
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A novel sec7 domain containing protein BIG3 and its role in regulated secretory pathway
... knockdown MIN6 cell line Figure4.2 Increased betagranin storage in BIG3-deficient MIN6 cells Figure4.3 Increased insulin storage in BIG3-deficient MIN6 cells ix Figure4.4 Increased betagranin secretion ... environmental inputs Endocrine cells, including beta cells, usually produce and process protein hormones by a common secretory pathway similar to other secreted proteins in all cells, but they also contain ... protein recruitment distiguishes the cargo-loaded subdomains from the rest of TGN Curvature-inducing proteins, such as the Bin/amphiphysin/Rvs (BAR) domain family and the GRIP-domain-containing...
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Characterization of helicobacter pylori y glutamyl transpeptidase and its role in pathogenesis
... genes coding for a range of pro-inflammatory mediators, including interleukin-6, interleukin-8, and tumor necrosis factor α (Schreck et al., 1991) The increased expression of these pro-inflammatory ... virulence factor rather than an essential factor in H pylori colonization Thus, the role of GGT in H pylori growth and colonization has not been clearly defined Introduction 1.3.1.2 GGT and H pylori-induced ... cells (AGS and KATO III) displayed an increase in H2O2 generation, activated NF-κB and IL-8 production However, preincubating these cells with NAC (an antioxidant), ST638 (a tyrosine kinase inhibitor)...
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Characterization of rab22b, an astroglia enriched rab GTPase, and its role in golgi and post golgi membrane traffic
... of protrudin promoted neurite formation in hippocampal neurons while the depletion of protrudin by RNA interference in PC12 cells inhibited NGF-induced neurite outgrowth, and resulted in membrane ... form myelin sheaths (Simons and Trajkovic, 2006) The microglia is the equivalence of 24 Introduction immune cells in the CNS, playing roles in CNS inflammatory responses and serving in phagocytotic ... recycling endosomes Dominant-negative Rab11A appears to inhibit apical recycling and basolateral-to-apical transcytosis in polarized Madin-Darby canine kidney (MDCK) cells (Wang et al., 2000) In...
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