uvra uvrb and uvrc proteins
Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... one strand and two AG steps in the other The mdm2 target, whose interaction with p53 was most strongly affected by DNA cis-platination, contains GG, GGG and AG motifs in one strand and GG and GTG ... p53 and p73 proteins [shown in Fig 2B for p53(1–363) and p73d], although their affinities for the proteins differed to some extent (which was manifested by different intensities of the R bands) ... To verify the specificity of the band shifts for DNA complexes with the proteins studied, we used the band supershift assay with antibodies against the p53 or p73 proteins Addition of the DO1 antibody...
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Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx
... Tat-containing recombinant proteins and call other reasons to explain the differences in the mechanism of internalization between the CPP peptide and the Tat-containing proteins Along this line, ... temperature, and washed again with NaCl/Pi before being processed in VectashieldTM mounting solution (Vector Laboratories Inc., Burlingame, CA, USA) Internalization and detection of recombinant proteins ... was supported by grants from the Association pour la Recherche sur le Cancer to B L and E V and from MURST and Istituto Superiore di Sanita¢, Rome, Italy to M G REFERENCES Allinquant, B., Hantraye,...
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Báo cáo khoa học: Dynamin-related proteins and Pex11 proteins in peroxisome division and proliferation doc
... domain, and the C-terminal GED (Fig 2) Obvious PRDs or PH domains are not found in DRPs Examples of DRPs are Mx proteins, mammalian DLP1, and the yeast proteins Dnm1, Mgm1 and Vps1 Mx proteins ... GIM5A and GIM5B These two proteins are nearly identical in sequence and show weak similarity to Pex11 Both are 26 kDa, have two putative 5172 S Thoms and R Erdmann transmembrane domains, and ... peroxisomal matrix and membrane proteins, yet contain vesicular structures with some peroxisomal proteins [61] Pex28 and Pex29 from S cerevisiae are also peroxisomal membrane proteins [62] They...
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Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot
... Disassembly and recruitment of hRad51 and hRad52 proteins shorter and thicker nucleoprotein filaments when ssDNA is added [28] Tumour suppressors BRCA2 and p53 interact with the ... was incubated in binding buffer in the absence (lanes and 3) or presence of 75 lM ssDNA (lanes and 5) and mM ATP (lanes and 5) for h at 37 °C and then subjected to partial digestion with trypsin ... lanes and with those in lanes 3, and reveals that the FEBS Journal 273 (2006) 199–207 ª 2005 The Authors Journal compilation ª 2005 FEBS 203 Disassembly and recruitment of hRad51 and hRad52 proteins...
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Báo cáo khoa học: Differential expression of liver and kidney proteins in a mouse model for primary hyperoxaluria type I pdf
... R Hernandez-Fernaud and E Salido Proteome changes in primary hyperoxaluria 4769 Proteome changes in primary hyperoxaluria ´ J R Hernandez-Fernaud and E Salido Fig Metabolic kidney (A) and liver ... peroxisomal (FP) and cytosolic (FC) fractions Densitometry of western blot bands was performed and units of selected proteins were calculated A representative western blot band for each protein ... protein expression within the liver and kidney of Agxt) ⁄ ) deficient mice 4768 ´ J R Hernandez-Fernaud and E Salido compared with wild-type controls by 2-DE separation and MS The analysis of specific...
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Báo cáo khoa học: Biogenesis of peroxisomes Topogenesis of the peroxisomal membrane and matrix proteins ppt
... challenge, as the proteins are transported in a folded or even oligomeric conformation Pex8p triggers the association of the docking and the RING-finger complex 2364 I Heiland and R Erdmann and might ... that are defective for the import of PTS1 and PTS2 proteins still import peroxisomal membrane proteins Thus, the import of peroxisomal membrane and matrix proteins is independent [41,42,72] The ... import of peroxisomal matrix proteins, and the mechanisms involved are becoming better understood [8,9] It is generally accepted that Pex5p and Pex7p, the receptors for the proteins harboring peroxisomal...
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applications of chimeric genes and hybrid proteins, part a
... tagging proteins for immunodetection, for using chimeric proteins for cytological purposes, especially the analysis of membrane proteins and intracellular protein trafficking, and for monitoring and ... hybrid genes and proteins to identify and analyze protein-protein and protein-nucleic interactions, for mapping molecular recognition domains, for directed molecular evolution, and for functional ... (B) Similarly, pRS551 and pRS550 are the same plasmid with the MCS in opposite orientations, and so on Plasmids pRS415 and pRS551 (and the corresponding plasmids pRS528 and pRS550) are designed...
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applications of chimeric genes and hybrid proteins, part b
... applications: molecular characterization of ligands and receptors, and the cloning of novel ligands and receptors Although we focus on polypeptide ligands and their cell surface receptors, the same ... receptor or ligand fusion protein probes focused on the identification and cloning of previously unknown ligands or receptors.4 More recently it has been found that receptor and ligand fusion proteins ... tagging proteins for immunodetection, for using chimeric proteins for cytological purposes, especially the analysis of membrane proteins and intracellular protein trafficking, and for monitoring and...
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báo cáo hóa học:" Proteomic characterization of HIV-modulated membrane receptors, kinases and signaling proteins involved in novel angiogenic pathways" ppt
... cytoplasmic proteins include KMLS and MAPK3 (MKO3) and nuclear proteins are TP53B and ZNF71 Full protein names, abbreviations and accession #s for each of all proteins are provided in Table Figure Proteins2 Detected ... binding proteins (GRB2, CRKL and NELL1), protease/ peptidase (ATS9 and C3/CO3), regulatory enzyme (NS2A), integral membrane proteins (TNR9 and GLG1), calcium-binding protein (ANX-A6) and coagulation ... domains of these proteins interact with catenin and other signaling proteins and activate enzymes, ion channels, a process that facilitates cell adhesion and migration [123,124,156] These proteins are...
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báo cáo hóa học:" Correlation between expression of p53, p21/WAF1, and MDM2 proteins and their prognostic significance in primary hepatocellular carcinoma" docx
... specimens and data analysis, and drafted the manuscript ZYZ and JF participated in the interpretation of data, conducted immunohistochemistry, and western blot analysis All authors read and approved ... p53, p21/WAF1, and MDM2 in HCC tissues than the corresponding adjacent hepatic tissues; the expression levels of the proteins in the former was 70.7%, 33.1%, and 52.5%, respectively and those in ... [24] and cutaneous squamous cell carcinoma [25], but is decreased in colorectal carcinoma [26] and ovarian carcinoma [27] Table 3: The expression of p53, p21/WAF1, and MDM2 in HCC tissues and...
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báo cáo hóa học: " Leader (L) and L* proteins of Theiler''''s murine encephalomyelitis virus (TMEV) and their regulation of the virus'''' biological activities" docx
... that capsid proteins, especially VP1 and VP2, are important for virus persistence and demyelination [1,3] In addition to these structural proteins, two proteins designated leader (L) and L* that ... envelope A singlestranded RNA is packaged in the shell that consists of four capsid proteins, VP1, VP2, VP3 and VP4 [13] Neutralizing epitopes have been identified [14-16], the nt and predicted AA ... contributions MT conceived this review and wrote the initial draft with KA under the direction of YO YO and TO modified, wrote and submitted the final draft All authors read and approved the final version...
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Báo cáo hóa học: " Respiratory syncytial virus (RSV) attachment and nonstructural proteins modify the type I interferon response associated with suppressor of cytokine signaling (SOCS) proteins and IFN-stimulated gene-15 (ISG15)" potx
... intracellular IFNα, IFNβ and SOCS1 and SOCS3 protein levels were determined at 24 h and 48 h pi by flow cytometry (Figure 2) At 24 h or 48 h pi, IFNα and IFNβ protein expression in RSV and RSV mutant virus ... cells to RSV infection and the role of RSV surface proteins in modulating this response The studies center on SOCS1 and SOCS3 negative regulation of the type I IFN response and ISG15 expression ... of SOCS3, ISG15 and IFNβ mRNA expression Results RSV stimulation of SOCS1, SOCS3, IFNα and IFNβ mRNA expression To determine the relationship between RSV infection, RSV proteins, and SOCS regulation...
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Báo cáo y học: "Diagnostic value and clinical laboratory associations of antibodies against recombinant ribosomal P0, P1 and P2 proteins and their native heterocomplex in a Caucasian cohort with systemic lupus erythematosus" pot
... PNH, PR0, PR1 and PR2 proteins (Figure 1), Sm and dsDNA (ELISA and Farr assays) were measured in sera from 163 SLE patients, 210 disease controls and 100 healthy donors to define and compare the ... heterocomplex (aRibPNH), aRibP R 0, aRibP R and aRibP R for SLE and to analyse their associations with disease features and future damage Materials and methods Study participants Altogether 479 ... (2.0%) and two (2.0%) patients had high titers of aRibPNH, aRibPR0, aRibP R2, RU/mL aRibPR1, RU/mL SLE n=163 C) Reactivity and diagnostic significance of antiribosomal proteins N, P0, P1 and P2...
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Báo cáo y học: "Rev and Rex proteins of human complex retroviruses function with the MMTV Rem-responsive element" doc
... Rem and Rex stimulated reporter expression 2-fold and 4-fold, respectively, and was dependent on the RmRE (Figure 4B) Western blotting showed similar expression of these proteins (Figure 4C and ... cellular proteins, such as hnRNPs and Sam68 [77, 78], and binding of these cellular proteins may determine the cell-type specificity observed in our experiments Since retroviral export/regulatory proteins ... Salmons B, Gunzburg WH and Indik S: Mouse mammary tumor virus integration site selection in human and mouse genomes J Virol 2008, 82:1360–1367 Andersson ML, Medstrand P, Yin H and Blomberg J: Differential...
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Báo cáo y học: " Determination of the relative amounts of Gag and Pol proteins in foamy virus particles" doc
... Gag(IN) Pol proteins, a cellular lysate (C), and extra-cellular virus (V) detected Immunoblot of SGG1 (Gag), 15E10 (RT), and 3E11 and Immunoblot of a dilution series of recombinant Gag and Pol proteins, ... bacterially-expressed Gag and Pol proteins were also applied to the gel The intensities of the bands were determined with a LAS-3000 (Fujifilm) and the relative amounts of Gag and Pol proteins were calculated ... the bands in the lanes with recombinant PFV proteins shown in Fig and amounts of protein Relation of the intensities of the bands in the lanes with recombinant PFV proteins shown in Fig and amounts...
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Báo cáo y học: "Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins" doc
... respect to DNA and MT-binding proteins: linker proteins require DNA binding proteins, and possibly also other linker proteins, for CEN DNA binding but not MTs or MT-associated proteins (MAPs) ... NDC80 complex (four proteins) , COMA (four proteins) , MIND (four proteins) and the SPC105 complex (two proteins) These complexes, in turn, recruit a multiplicity of motor proteins and MAPs to form ... subunits were invariably regulatory proteins such as the Mad and Bub checkpoint proteins and the Aurora B kinase Structural proteins such as Ndc80Hec1, Nuf2, CENP-CMif2 and Mis12Mtw1 were considerably...
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Studies on interactions between HCRSV coat protein and host proteins from kenaf
... host responses and the signaling networks that are involved In addition, for most viral proteins, the mechanisms of the interaction between different host proteins and viral proteins and the meaning ... affected host gene expression and proteins The virus diverts a substantial amount of plant metabolites for the production of virus specific proteins and nucleic acids, and alters host gene expression ... thaliana And H2O2 accumulation was abolished in NADPH oxidase rbohD and rbohF (rbohDF) double mutants and in two ethylene response mutants (etr1-1 and ein2-1 mutants), implicating NADPH oxidase and...
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X ray crystallographic study of yeast dcp1 and dcp2 proteins insights into the mechanism and regulation of eukaryotic mRNA decapping
... SMG5, SMG6 and SMG7 proteins are required for proper regulation (Conti and Izaurralde, 2005) The NMD factors can interact with the mRNA decay machinery and target mRNA for decapping and Xrn1 digestion ... shortening (Lejeune and Maquat, 2005; Muhlrad and Parker, 1999) Alternatively, mRNA can undergo rapid deadenylation and be degraded by the exosome and Ski complex (Mitchell and Tollervey, 2000) ... Lykke-Andersen 12 and Wagner, 2005) HuR is another ARE-binding protein but plays an antagonizing role It binds to ARE and poly(A) regions of target mRNA and prevents it from degradation (Brennan and...
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Crystallization trials of refolded breast tumor kinase (BRK) and peroxisome proteins
... protein import The PTS1 and PTS2 receptors and their accessory proteins (e.g Pex20p) ferry cargo from the cytosol and first interact with the docking subcomplex (Heiland and Erdmann, 2005) The ... (EGF) and serum, via EGFR, erbB3 and possibly other cell-surface receptors to changes in phosphoinsitide 3-kinase, Akt, paxilin and Rac activities and interactions In the Tcell lymphoma and other ... GST-Pex8 and pH 7.5 for GST-Pex20, filtered] at a flow rate ml/min in an AKTA system The GST-Pex8 and GST-Pex20 proteins were concentrated to mL and injected into the FPLC system Each of these proteins...
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Crystallization trials of refolded breast tumor kinase (BRK) and peroxisome proteins 1
... 1.2.1 X-ray crystallography for proteins BASIC CONCEPTS IN CRYSTALLOGRAPHY 1.3.1 1.3 Unit cell and lattices 1.3.2 Symmetry, point group and space group 1.3.3 Crystals and X-rays 1.3.4 X-ray diffraction ... ROLES OF PEX8 AND PEX20 IN PICHIA PASTORIS 23 2.2.1 Peroxisome biogenesis and degradation 23 2.2.1.1 Peroxisomal constituents and its functions 23 2.2.1.2 Basic views of Lipid and Protein Import ... identity for GST-Pex8 and GST-Pex20 proteins 41 3.2.6 Protein concentration identification 42 CHAPTER RESULTS AND DISCUSSION 4.1 4.2 WT-BRK REFOLDING, STRUCTURE ANALYSIS AND CRYSTALLIZATION 43...
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