using peptide loop insertion mutagenesis for the evolution of proteins
Báo cáo khoa học: Distribution of the extrinsic proteins as a potential marker for the evolution of photosynthetic oxygen-evolving photosystem II ppt
... propose a new model for the evolution of the PSII extrinsic proteins (Fig 5) The prokaryotic cyanobacteria contain five genes for 5027 Evolution of PSII extrinsic proteins the PSII extrinsic protein ... with the result of Shibata et al [21] The presence of PsbV in C paradoxa is consistent with the presence of the psbV gene in the complete sequences of the cyanelle genome [24], in which the psbU ... detect the presence of PsbU in these algae which plays a role in optimizing the availability of Cl– cofactors for water oxidation [5,29] This may be due to the high species-specificity of the antibody...
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báo cáo khoa học: " Pollen development in Annona cherimola Mill. (Annonaceae). Implications for the evolution of aggregated pollen" pdf
... concomitantly with the layering of the exine [54] and the formation of the pollen aperture is related to endoplasmic reticulum blocking the deposition of primexine [3] The callose remnant at the pollen ... cherimola, the confined space that permits the delay in the dissolution of the PMC wall and the tapetal chamber and pollen rotation that allows the adhesion of the sticky proximal faces by the formation ... and the first pollen mitosis and vanished with the onset of these two divisions; this also occurred days before anther dehiscence, preceding the shedding of starchless pollen The accumulation of...
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Designing listening tasks using authentic materials on websites as supplementary materials for the teaching of listening skills
... answer of up to 83% of the students when asked There are about 42% of the students who say that their teachers of listening skills make use of ready-made tasks using the recordings from the Internet ... listener-based The listener privies to It is text-based The listener relies on the background knowledge of the topic, the situation language in the message, that is, the or context, the type of text, and the ... half of them (52.3%) express the wish to take part in the process of building up the supplementary listening materials They would rather their teachers give them opportunities to design the tasks...
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Báo cáo khoa học: A novel factor XI missense mutation (Val371Ile) in the activation loop is responsible for a case of mild type II factor XI deficiency doc
... shown The conformational movements of Ile371, located in the activation loop at the N-terminus of the catalytic domain, are notable In the zymogen FXI, Ile371 is exposed to the solvent, while in the ... contrast to the activated FXI, in the structure of the FXI zymogen, Val371 is located on a loop region, exposed to solvent, and does not form many contacts with other residues (Fig 6) Therefore, the ... explained by the proximity of the mutation to the cleavage site, probably resulting in a small interference with the binding of the activator to the FXI zymogen There are some examples of inherited...
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Báo cáo khoa học: A strategy for the generation of specific human antibodies by directed evolution and phage display An example of a single-chain antibody fragment that neutralizes a major component of scorpion venom docx
... of mouse weight) [13] Clone 3F was matured by three cycles of directed evolution The use of a set of stringent conditions in the third cycle allowed the selection of several improved clones The ... C noxius venom To the best of our knowledge, this is the first report of the generation of a human recombinant antibody fragment capable of neutralizing the toxic effects of the whole venom from ... corresponding germ lines For clone 3F, VH3-VK3 were the closest families for VH and VL domains, respectively In the case of C1, VH3Vk1 were the families with highest scores The specificity of these two scFvs...
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Báo cáo khoa học: "AN EXPERT SYSTEM FOR THE PRODUCTION OF PHONEME STRINGS FROM UNMARKED ENGLISH TEXT USING MACHINE-INDUCED RULES" pdf
... is then selected on the basis of values of the word features The process is repeated until a leaf node of the tree is reached The leaf node contains the value returned for that invocation of ... the basis of the values of the word's word level attributes Application of the stress rule also returns either or -I The training mode operates in the same manner as the execution mode with the ... the content of attrlbute-vaiues might be information When generating rules using UTTER the user has the option of using either only a test for equality in the decision tree, or a larger set of...
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Báo cáo khoa học: Utility of epimerization domains for the redesign of nonribosomal peptide synthetases pdf
... Although the focus of this study is not the engineering of hybrid–NRPS enzymes the results obtained on the substrate specificity of E domains could pave the way to engineering of biosynthesis of valuable ... (Fig 4) The relative values of l- ⁄ d-isomers were calculated easily by determining the area of the ion extracted MS signals Therefore, the use of an internal standard was not necessary for quantification ... alkaline cleavage of the thioester and analysed by HPLC The amount of each isomer was quantified and the formation of the D(R2)-peptides (in percentage) presented in dependence of time basic [FH-CoA...
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a novel method for the synthesis of titania nanotubes using
... when using magnetic stirring The saturation of current with time indicates the development of repassivation, the saturation of nanotube formation These results are in line with the findings of our ... formation continuing until repassivation occurs [17,19] Nanotube formation goes through the diffusion of F− ions and simultaneous effusion of the [TiF6 ]2− ions The faster rate of formation of ... using ultrasonic waves can be explained by the faster mobility of the F− ions into the nanotubular reaction channel and effusion of the [TiF6 ]2− ions from the channel It is well known that the...
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Báo cáo sinh học: " A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols" pot
... collaboration with the CIC executive office and the steering committee of the CIC Immunoassay working group The authors of this group acknowledge the concept of the Minimal Information About T ... did the final approval of the manuscript MK, MP, LMcN, TC, JY, KO and AH were driving the conception and design of the study, selected the donors for the study, interpreted the data and wrote the ... obtained using the irrelevant MULTIMERS in four donors stratified by DUMP channel use and further subdivision by the use of dead cell marker The table also indicates the number of labs (N) for each of...
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báo cáo hóa học: " The evolution of methods for the capture of human movement leading to markerless motion capture for biomechanical applications" pptx
... knowledge related to the mechanics of human movement The work at the University of California formed the basis for many of the fundamental techniques currently used for the study of human locomotion ... there was a need for an improved understanding of locomotion for the treatment of World War II veterans The classic work at the University of California [5,6] provided a tremendous resource of ... limiting the advancement of the study of human movement is the measurement of skeletal movement from markers or sensors placed on the skin The movement of the markers is typically used to infer the...
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o cáo hóa học:" A critical assessment for the value of markers to gate-out undesired events in HLA-peptide multimer staining protocols" pot
... collaboration with the CIC executive office and the steering committee of the CIC Immunoassay working group The authors of this group acknowledge the concept of the Minimal Information About T ... did the final approval of the manuscript MK, MP, LMcN, TC, JY, KO and AH were driving the conception and design of the study, selected the donors for the study, interpreted the data and wrote the ... obtained using the irrelevant MULTIMERS in four donors stratified by DUMP channel use and further subdivision by the use of dead cell marker The table also indicates the number of labs (N) for each of...
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Báo cáo hóa học: " A rapid, convenient, solventless green approach for the synthesis of oximes using grindstone chemistry" docx
... furnish the pure oxime in 60-98% yield 90 Page of run might be due to the loss of active sites of the catalyst Conclusions To the best of our knowledge, Bi O has never been used in the synthesis of ... grounded in a mortar with a pestle for the required period of time On completion of the reaction as Table Reusability of Bi2O3 in the preparation of 2b using 60 mol% of the catalyst Entry Run number ... in the absence of a base or any other additives To search for the best reaction condition for oximation using easily available bismuth compounds, a set of reactions have been carried out using...
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Báo cáo sinh học: "Comparative embryology without a microscope: using genomic approaches to understand the evolution of development" potx
... Richards RJ: The Meaning of Evolution: the Morphological Construction and Ideological Reconstruction of Darwin’s Theory Chicago: University of Chicago Press; 1992 Raff RA: The Shape of Life: Genes, ... developmental divergence and the strength of selection (a) Two potential patterns of conservation at the level of embryonic development The grey line represents the classical pattern of early developmental ... with those along the black lineages, we can get a sense of the ways in which changes in maternal contribution influence the evolution of development at a genetic level was stronger for genes expressed...
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Báo cáo khoa học: " Effective methods for the production of immunoglobulin Y using immunogens of Bordetella bronchiseptica , Pasteurella multocida and Actinobacillus pleuropneumoniae" docx
... volume of PBS The homogenized egg yolk was then mixed with two volumes of chloroform and incubated for hrs at room temperature After this incubation, the supernatant was collected To compare the ... patterns in the analysis of antibody titer by ELISA using IROMP and OMP of P multocida A:3 (Fig and 6) However, the response in group was lower than in the other groups in case of using IROMP as a coating ... Co., Germany) for 30 seconds After centrifugation of the sonicated bacterial culture broth at 8,000 rpm for 30 min, the supernatant was centrifuged at 20,000 rpm for hr at 40C and the pellet was...
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Báo cáo khoa học: "Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections" pdf
... recommended for diagnosis of CHV in formalin-fixed tissues The optimized ISH assay, which developed in this study, may be a valid tool for the study of pathogenesis and diagnosis of CHV infection ... digoxigenin-labeled probe is better than those of biotin-labeled probe in the hybridization assay for the detection of CHV These results suggest that ISH assay using digoxigenin-labeled probe, which ... hybridization for CHV In this study, several ISH protocols, which were consisted of the changes of enzyme-concentrations, the time of hybridization and hybridization probes, were compared in formalin...
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Báo cáo khoa học: " Development of a sandwich ELISA for the detection of Listeria spp. using specific flagella antibodies" potx
... monitor the development of the antibody level using the yolk diluted 1,000 times with PBST The yolk antibody also showed no cross reaction to any other bacteria (Table 1), and the antibody levels of ... combinations at 7,000 rpm for 30 After centrifugation at 14,000 g for 40 of the supernatant from previous centrifugation, the flagella of pellet were resuspended in PBS and stored at 20oC The protein concentrations ... buffer (pH 9.6) to the concentration of 10 µg/ml and 100 µg of diluted MAb was added into ELISA plate (Costar, Netherlands) The plate was incubated for h at 37oC and overnight at 4oC The wells were...
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Báo cáo lâm nghiệp: "An approach for the analysis of vegetation spectra using non-linear mixed modeling of truncated power spectra" pdf
... reflectance in the visible yet reduced reflectance in the NIR and SWIR regions of the spectrum Analysis of vegetation spectra 519 Figure Summary of F-ratio tests of significance of the variance of the ... differences in these factors are also conjectured for the eucalypt foliage These a priori expectations were tested with the null hypothesis of no treatment viz no species effect With the assumption of a ... The choice of r – degrees of freedom in the numerator of the F-ratio test instead of was adopted to keep the experiment-wide error rate at 0.05 or better [38] The number of retained frequencies...
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Báo cáo y học: "A 12 Week, Open Label, Phase I/IIa Study Using Apatone® for the Treatment of Prostate Cancer Patients Who Have Failed Standard Therapy" pps
... decrease The binomial expansion was used to calculate the exact probability of the number of successful outcomes among the enrolled patients A probability of p
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Báo cáo khoa học: "Transarterial chemoembolisation (TACE) using irinotecan-loaded beads for the treatment of unresectable metastases to the liver in patients with colorectal cancer: an interim report" pdf
... date of diagnosis of metastases was 3.4 years the median survival time from the start of TACE treatment was 1.34 years The median survival time of the palliative group was 1.4 years, of the symptomatic ... patient The average age of the 207 patients was 68.8 years (range, 39.4-83.5 years) Of these, 158 were treated for palliation, 35 to reduce symptoms, and 14 as adjuvant therapy The chemotherapy ... assessment of tumor response was performed for all patients by the Principal Investigator at University of Louisville When there was a discrepancy, the Registry PI and the site PI reexamined the data...
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Báo cáo y học: "A critical appraisal of guidelines for the management of knee osteoarthritis using Appraisal of Guidelines Research and Evaluation criteria" doc
... guidelines for therapeutic exercises and manual therapy in the management of osteoarthritis Phys Ther 2005, 85:907-971 American College of Rheumatology: Recommendations for the medical management of ... category of intervention into various forms, and others succinctly describing only the category The interests, mandate and resources of the development team probably guided the type and extent of interventions ... related to the weaker evidence regarding the effectiveness of these interventions The role of these interventions should be specified in future guidelines AGREE evaluation of guidelines The AGREE...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25