user friendly expression plasmids enable the fusion of vhhs to application speci fi c tags
Báo cáo Y học: Expression pattern in the antennae of a newly isolated lepidopteran Gq protein a subunit cDNA potx
... gene -speci c primer, designed according to the cDNA sequence obtained from the internal amplification (5¢-TCGCCTGCCGTCGTAGCAC TCCTG -3¢) The 50-lL amplification mix was prepared according to the ... characteristics, the molecular cloning of the corresponding cDNA to get the total amino-acid sequence of the protein, and the expression pattern of the corresponding mRNA as a first step to clarify the ... then cycles of 30 s at 94 C, 30 s at 70 C and at 72 C, then 25 cycles of 30 s at 94 C, 30 s at 68 C and at 72 C, then at 72 C Cloning and sequencing The amplified cDNAs were ligated into...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... Prediction of plant microRNA targets Cell 2002, 110(4):513-520 Llave C, Xie Z, Kasschau KD, Carrington JC: Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA Science ... Loss -of- function of the miR-17-92 cluster resulted in smaller embryos and immediate postnatal death of animals [67], which could due to the deficiency of their roles in the development of the ... related to chromatin structure were identified in this cluster, which included histone modification, covalent chromatin modification, establishment and or maintenance of chromatin architecture, chromosome...
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Báo cáo hóa học: "Prognostic impact of clinical course-specific mRNA expression profiles in the serum of perioperative patients with esophageal cancer in the ICU: a case control study" potx
... AAC CAC CCA GAT GCT TCA GT TCC GCT TTT GCC ATA TCT TC TNF-a MN_000594.2 TGC TTG TTC CTC AGC CTC TT GCA CTC ACC TCT TCC CTC TG MUC1 MN_001018016.1 CCA TTC CAC TCC ACT CAG GT CCT CTG AAG GAG GCT ... ACA GCG ACA AGA AGT GG CCC TCA GTC AAG CGC TAC AT IL-6 MN_000600.3 ATG CAA TAA CCA CCC CTG AC TAA AGC TGC GCA CAA TGA GA b-actin MN_031144.2 ACC TGA CTG ACT ACC TCA TG GCA GCC GTG GCC ATC TCT ... TTC CTG AGG GCA TTG TC GGC CAC TGT GAT TGG ATA CC CRP EGR1 MN_000567.2 MN_001964.2 ACA GTG GGT GGG TCT GAA AT TTC TTC GTC CTT TTG GTT TA TAC CCA GAA CTC CAC GAT CC CTT AAG GCT AGA GGT GAG CA...
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Báo cáo toán học: " Pyramid-based image empirical mode decomposition for the fusion of multispectral and panchromatic images" pptx
... residual The first IMF of the panchromatic image replaces the first IMF of the MS image Finally, the fused image is obtained by reconstructing the mixed IMFs of the MS image The reconstruction process ... decomposition, which can also reduce the computation time of IMFs extraction In addition, the advantage of an openness operator is the ability to accurately extract the extreme points of scales with ... image fusion EMD-based image fusion is similar to the traditional wavelet approach The process uses a high-frequency panchromatic IMF to replace the high-frequency IMF of MS images Then the IMFs...
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Báo cáo hóa học: " The Fusion of Distributed Microphone Arrays for Sound Localization" pdf
... observabilities into account This time, the location of the speakers can be clearly determined Each of the three peaks correspond to the correct location of their corresponding speakers For the experiments ... points and the velocity of sound in air can be used to estimate the difference in the distances of the observation points to the sound source location The difference in the distances constrains the sound ... Assistant Professor in the Edward S Rogers Sr Department of Electrical and Computer Engineering at the University of Toronto, and the Founder and Director of the Artificial Perception Laboratory Professor...
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Báo cáo y học: "Cigarette Smoke Exposure Alters mSin3a and Mi-2α/β Expression; implications in the control of pro-inflammatory gene transcription and glucocorticoid function" ppt
... by nuclear hormone receptors including the glucocorticoid receptor α (GRα) [10-15] HDACs function by deacetylating of key components of the transcriptional machinery including the core histone ... seen in the lungs of sham exposed controls This may be part of a positive feedback mechanism by which glucocorticoids increase the availability of co-repressor complexes to further enhance the GR-mediated ... bringing functional co-repressors such as HDAC1/2 to sequence specific transcription factors and thereby allowing functional repression of gene transcription and a reduction in their expression...
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Báo cáo y học: "Cigarette Smoke Exposure Alters mSin3a and Mi-2α/β Expression; implications in the control of pro-inflammatory gene transcription and glucocorticoid function" pdf
... by nuclear hormone receptors including the glucocorticoid receptor α (GRα) [10-15] HDACs function by deacetylating of key components of the transcriptional machinery including the core histone ... seen in the lungs of sham exposed controls This may be part of a positive feedback mechanism by which glucocorticoids increase the availability of co-repressor complexes to further enhance the GR-mediated ... bringing functional co-repressors such as HDAC1/2 to sequence specific transcription factors and thereby allowing functional repression of gene transcription and a reduction in their expression...
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Báo cáo y học: "Effects of typical and atypical antipsychotic drugs on gene expression profiles in the liver of schizophrenia subjects" docx
... Thermo cycle conditions were: (1) cycle for at 50 C, (2) cycle for 15 at 95 C, and (3) 40 cycles for 15 sec at 95 C and at 60 C and fluorescence was measured during the 60 C step for each cycle ... average correlation For each metric, we computed the distribution of the metric across the samples within each study Although no hard cutoffs were applied for each of the QC metrics, we examined the ... atypical AP vs control, and typical AP vs atypical AP Comparison Category Term Typical vs Ctrl Typical vs Ctrl Typical vs Ctrl Typical vs Ctrl Typical vs Ctrl Atypical vs Ctrl Atypical vs Ctrl...
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báo cáo khoa học: " Integration of tomato reproductive developmental landmarks and expression profiles, and the effect of SUN on fruit shape" pot
... inflorescence Characterization of the S pimpinellifolium accession LA1589 Characterization of the S pimpinellifolium accession LA1589 inflorescence (A) Series of consecutive floral buds (right) to ... response to other hormone cues such as cytokinin In Arabidopsis, CYCD3;1 responds to cytokinin to activate cell division at the G1-S cell cycle phase [83] After establishing the morphological landmarks ... initiation, the generative cell displayed the characteristic crescent shaped nucleus (Fig 3L and 3M) The second mitosis of the generative cell did not occur until after pollination Female reproductive...
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Báo cáo y học: "Silencing CD36 gene expression results in the inhibition of latent-TGF-β1 activation and suppression of silica-induced lung fibrosis in the rat" pdf
... Carlsbad, CA, USA) according to the manufacturer's protocol The sequences of primers specific for CD36 (sense: 5'-GAAGCACTGAAGAATCTGAAGAG-3'; antisense: 5'-TCCAACACCAAGTAAGACCATC-3'), and βActin ... TSP-1 The TSP-1/L-TGF-β1 complex associates with CD36 on cell surface via the specific interaction between the YRVRFLAKENVTQDAEDNC (93–110) sequence of CD36 and the sequence CSVTCG (447–452) of ... collagen I of the silica+LvshCD36 group were significantly lower than those of the silica and the silica+Lv-shCD36-NC groups (B) The IOD averages of collagen III of the silica, the silica+Lv-shCD36...
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Báo cáo y học: " Individual mRNA expression profiles reveal the effects of specific microRNAs Amit Arora and David AC Simpson" pot
... given the wide range of regulatory mechanisms acting on gene expression, we are able to detect the effect of miRNAs on their diverse collection of target genes The effectiveness of three analytical ... shown to increase site efficacy [2] In a preliminary attempt to characterize the distinguishing properties of the potential classes of miRNAs described above, we analyzed the lengths of contiguous ... chose to initially use TargetScan [27] because its requirement for a perfect match to the seed region and cross-species conservation reduce the falsepositive rate [3-5] The resulting higher specificity...
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Báo cáo y học: " Gene expression regulation in the context of mouse interspecific mosaic genomes" pdf
... peculiarity of our model: the homozygosity of the M spretus segments, which obliges trans-regulators of one species to act on cis-elements of another species In the hybrid, by contrast, the co-existence ... function When the different classes of genes were compared with respect to their testis-specific gene content, only classes and (encompassing only 4% of testis-specific genes) were significantly ... years ago, accumulating an interspecific divergence estimated at 1% [16,25] The process to obtain the IRCSs involved an interspecific cross followed by two backcrosses on a M musculus background...
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Using biological networks and gene expression profiles for the analysis of diseases
... Ovarian cancer dataset Predictive accuracy of gene feature-based classifier compared to bagging Predictive accuracy of gene-feature-based classifier compared to PFSNet and ESSNet classifier ... net contain values that agree with the actual data GGEA compares these values to compute a consistency score The consistency score of a pathway is the sum of individual genes’ consistency in the ... and ESSNet to achieve high cross-batch prediction accuracy 1.3 Thesis organization Chapter provides technical background on microarray analysis methods, which try to identify the cause of a disease...
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DEVELOPING, USING AND ANALYZING THE RESULTS OF INTERVIEWS TO DETERMINE THE FEELINGS OF FOREIGN TOURISTS ON THEIR VISIT TO VIET NAM
... ability of working, of researching, etc With this HD2 project, we also get more chances to work in-group, but in this topic, we get a new way to this assignment It brings us a chance to communicate ... to discover by themselves, although they have much money to buy package tours The tendency of travelling alone becomes popular in Vietnam However, as you know, they are not the potential customer ... American tourists with 42% There are smaller percentages of European and Oceanian tourists They occupy 33% and 25% It is not difficult to explain for this result As you can see, there is a vacation...
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Tài liệu The Global Report of the Economics of Adaptation to Climate Change Study: Consultation Draft doc
... or manage resources in ways that take account of the potential impacts of climate change Often, the reluctance to change reflects the political and economic costs of changing policies and (quasi-) ... related to systemic changes, such as the melting of the Greenland and West Antarctic ice sheets, the collapse of the Atlantic thermohaline circulation, and the die-back of the Amazon, all hard to ... more common, use of the term captures the notion that poor countries have less capacity to adapt to change, whether induced by climate change or other factors, because of their lower stage of development...
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developing, using and analyzing the results of interviews to determine the feelings of foreign tourists on their visit to viet nam
... from the chart that almost tourists evaluated the quality of accommodation facilities in Hanoi are good, it occupies 67% in total The percentage of excellent facilities holds 17% And the percentage ... traditional cultures 15 Appreciations of quality of tourist services There are some small differences in this chart We did not divide the tourist services too clearly, but we generally gathered the tourist ... shown in the chart, the percentage of reason for beauty and others reason are equal, occupy 42% in total And the number of tourist choose Vietnam for cheap and strange have the same pecentage:...
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Tài liệu Báo cáo khoa học: An engineered disulfide bridge mimics the effect of calcium to protect neutral protease against local unfolding docx
... chevron plots act as an indicator for the occurrence of the intermediate in the unfolding Calcium ions are the main contributory factor to enzyme stability The binding of calcium ions seems to ... Unfolding of pWT and G 8C ⁄ N6 0C at the standard calcium concentration of mm was monitored by farUV CD spectroscopy, showing conformational changes of the secondary structure, and by fluorescence spectroscopy, ... one of the four calcium ions is bound very efficiently In the presence of less than mm CaCl2, pWT and G 8C ⁄ N6 0C unfold with the same rate constant at 7.25 m GdnHCl Only when the calcium ion concentration...
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Tài liệu The economics of moving to higher welfare farming docx
... thought to correct the market outcome back to efficiency 19 THE ECONOMICS OF MOVING TO HIGHER WELFARE FARMING VII CONCLUSIONS The additional farm level costs of producing food to higher standards of ... internalise the externalities of the production of meat and dairy products – i.e including them in the price of the product This would involve the introduction of a Pigouvian Tax equal to the cost of the ... eggs cost 94.31 pence to produce while the cost of producing a dozen cage eggs is 69.34 pence.2 Turning to the cost of producing one egg, one free-range egg costs 7.86 pence to produce and one cage...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
... study The lectin is unique from that of other sialic acidspeci c lectins O-Acetyl sialic acid -speci c lectin was Ó FEBS 2003 A sialic acid speci c lectin from P jacquemontii (Eur J Biochem 270) ... erythrocytes was marked by a 9-O-acetyl sialic acid -speci c lectin purified from the hemolymph of the snail Achatina fulica [64,66] Lectins are used for verification of the sugar speci city of the auto-antibodies ... binding speci city of crab lectin Inhibition studies with various sugars was helpful in deducing the binding speci city of the lectin Fucose and lactose inhibited HA activity of purified lectin Sucrose,...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25