useful control properties methods and events
HERBICIDES – PROPERTIES, SYNTHESIS AND CONTROL OF WEEDS pdf
... chapters deal with the synthesis and properties of herbicides and weed control I hope therefore that readers who are interested in a synthesis and properties and control of weeds, for example in ... majority of herbicides and pesticides are artificially synthesized in the labs; investigating synthetic pathways and properties, and that all products produced to overcome weeds and insects which ... Herbicides MCPA and 2,4-D in the Highest World Standard 347 Wiesaw Moszczyski and Arkadiusz Biaek Chapter 20 Vegetative Response to Weed Control in Forest Restoration John-Pascal Berrill and Christa...
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Báo cáo khoa học: "Genetic control of pulp and timber properties in maritime pine (Pinus pinaster Ait.)" docx
... ability (SCA) of the cross between the i-th and the j-th parent and εijk is the residual term The additive and dominance variances are:σ = 4σ and σ = 4σ and and the phenotypic variance is: A a D s ... significant impacts on pulp and timber production and quality 4.2.2 Relationships between fibre properties and wood chemical composition In the literature, fibre properties and the chemical composition ... heritabilities and genetic correlations are presented and a breeding strategy for the utilisation of maritime pine wood for timber and pulping purposes is discussed MATERIALS AND METHODS 2.1 Experimental...
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Supply chain engineering useful methods and techniques (2010) alexandre dolgui, jean marie proth
... Engineering Alexandre Dolgui · Jean-Marie Proth Supply Chain Engineering Useful Methods and Techniques 123 Prof Alexandre Dolgui Ecole des Mines de Saint-Étienne Centre for Industrial Engineering and Computer ... variability of demand and heterogeneity of customers 1.2 Definitions and Notations Production cost is the sum of fixed and variable costs Fixed costs include maintenance, wages and upkeep Note ... production systems In this chapter, their usefulness is highlighted and various functions and equipment are analyzed Recent advances such as the valueadded services and their corresponding areas are...
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Glycoprotein Methods and Protocols - P1
... hexose and sialic acid; membrane-based methods such as slot-blotting and staining with periodic acid-Schiff reagent; antibodies and lectins or coating methods such as the glycan detection method and ... carbohydrate and antibody analyses 3.6 Analysis of Mucins Methods for the detection and analysis of mucins are dealt with in other chapters in this volume However, three principally different methods ... remaining tissue From: Methods in Molecular Biology, Vol 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A Corfield © Humana Press Inc., Totowa, NJ Davies and Carlstedt To isolate...
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Glycoprotein Methods and Protocols - P2
... M and Milton, J D (1986) Lectin Methods and Protocols, Humana, Totowa, NJ Liener, I E., Sharon, N., and Goldstein, I J (1998) The Lectins: Properties, Functions, and Applications in Biology and ... mammary gland, defined by antibodies to cytokeratins J Cell Sci 75, 17–33 Kiernan, J A (1990) Histological and Histochemical Methods: Theory and Practice, Pergamon, Oxford, UK Andrew, S M and Jasani, ... sialosyl Tn and Tn antigens within normal and malignant colorectal epithelium J Pathol 176, 143–149 Histological Methods for Detection of Mucin 43 21 Jass, J R and Smith, M (1992) Sialic acid and epithelial...
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Glycoprotein Methods and Protocols - P3
... core protein specificity), and lower sensitivity than slot-blot and immunoassay methods Chap- Detection and Quantitation of Mucins 47 ter (2) discusses these assays and they are mentioned here ... greater sample volume, and superior washing) For quantitation and comparison across blots, a standard in the same buffer as samples should be titrated for use as a standard curve, and samples should ... NJ Nagma, K., Thornton, D J., Khan, N., and Sheehan, J K (2000) Separation and identification of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T.,...
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Glycoprotein Methods and Protocols - P4
... rat (and possibly other experimental animal models), is particularly useful for validating other in vitro methods for measuring mucous thickness, for studying mucous secretion in vivo, and for ... and held together by bolts tightened by hand Millipore filter paper to act as a backing for the mucosa during sectioning and subsequent manipulation of the section 2.2 Histological Staining and ... (w/v) poly-L-lysine for and dried at room temperature Methods 3.1 Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections (see Notes 1–4) This is a rapid and simple method for...
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Glycoprotein Methods and Protocols - P5
... PhosphorImager The identification of mucin precursors and mature mucins using polypeptide-specific antisera and SDS-PAGE is elaborated in Chapters 20 and 21, and in several references (1,4–11) The precursor ... Strous, G J and Dekker, J (1992) Mucin-type glycoproteins Crit Rev Biochem Mol Biol 27, 57–92 Van Klinken, B J W., Dekker, J., Büller, H A., and Einerhand, A W C (1995) Mucin gene structure and expression ... Einerhand, A W C., and Dekker, J (1995) Biosynthesis of intestinal mucins: MUC1, MUC2, MUC3, and more Biochem Soc Trans 23, 814–818 11 Van Klinken, B J W., Einerhand, A W C., Büller, H A., and Dekker,...
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Glycoprotein Methods and Protocols - P6
... a small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer Leave to cool before pouring (hand hot) and insert well-forming comb (see ... are 1.5 mm thick and cm wide There is a compromise between band broadness and amount of sample to be loaded Larger amounts of sample tend to yield poorer quality data; i.e., the bands become more ... I., and Sheehan, J K (1996) Identification of glycoproteins on nitrocellulose membranes and gels Mol Biotechnol 5, 171–176 10 Thornton, D J., Howard, M., Devine, P L and Sheehan, J K (1995) Methods...
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Glycoprotein Methods and Protocols - P7
... Kadler, K E., and Chapman J A (1985) Mica sandwhich technique for preparing macromolecules for rotary shadowing J Ultrastruct Res 91, 66–76 14 Kawahara K and Tanford C (1966) Viscosity and density ... temperature and mechanical instabilities in centrifuges We first demonstrated the value of rate-zonal centrifugation for the characterization of cervical mucin heterogeneity and polydispersity (5) and ... (Fullerton, CA) SW 40Ti swing-out rotor, and finally unloading and analysis of the tube contents Measure out 18 mL of M GuHCl into one chamber of the gradient maker and an equal volume of M GuHCl into...
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Glycoprotein Methods and Protocols - P8
... 114 Yan and Packer chromatography, and leads to a significantly lower recovery of all the amino acids, especially serine and threonine Strong acid, high temperature, and short time acid ... a C18 reversed-phase column and detected by fluorescence Our routine analyses have shown that the method described here is reliable and useful for both quantitative and qualitative mucin glycoprotein ... at least for pump and column equilibration with the initial composition of the mobile phase Figures and show typical chromatograms of the separation of amino acid standards and hydrolyzed bovine...
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Glycoprotein Methods and Protocols - P9
... Notes and 8) and the length of sequence obtained is dependent on the number and distribution of proline residues Proline cleaves with reduced efficiency in Edman sequencing (Fig 1, vessel 1), and ... like to acknowledge the support and useful comments of Dr Nicolle Packer and Prof Keith Williams (Macquarie University) References Rademaker, G J., Haverkamp, J., and Thomas-Oates, J (1993) Determination ... I., Kirk, M., Barnes, S., Lowary, T L., and Hart, G W (1996) Selective detection and site-analysis of O-GlcNAc-modified glycopeptides by β-elimination and tandem electrospray mass spectrometry Anal...
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Glycoprotein Methods and Protocols - P10
... tandem repeat domain and overlapping each other by six amino acids (16), and the other used octamers overlapping each other by seven amino acids (17) Twelve antibodies were analyzed in total and ... information on antibody specificity and permits identification of significant features of an epitope that may contribute to antibody recognition and binding (see Notes 10 and 11) 1.3 Purification of ... nuclear magnetic resonance spectroscopy, and computational molecular modeling are essential if the knowledge gained is to be confirmed and translated into a useful model on which to base antibody...
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Glycoprotein Methods and Protocols - P11
... lysates and media Nonspecific bands may be present and can be eliminated from consideration using the pSVL vector control lanes for comparison (Fig 2A,B, lanes and 3) The sizes of specific bands ... polymerase buffer, 2.5 mM MgCl2, 400 µM dNTPs, 200 ng of primers and or and 4, ng of template DNA (with only primers and 4), and U of Taq polymerase Perform a PCR program in a suitable thermal ... polymerase, and incubate the reaction at 72˚C to allow extension of the linked templates Add µg each of primers and 6, and continue the PCR with 30 cycles at 94˚C for 30 s, 50˚C for and 72˚C for...
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Glycoprotein Methods and Protocols - P12
... gas Program the oven temperature from 150 to 230°C and 3°C/min and then 230 to 250°C at 5°C/min Use injector and detector temperatures of 240 and 250°C, respectively Use an injection volume of ... Nakamura, M., and Ohkura, Y (1987) Fluorometric high performance liquid chromatography of N-acetyl- and N-glycolyl neuraminic acids and its application to their microdetermination in human and animal ... 164 Michalski and Capon Fig HPLC separation of sialic acid quinoxalinones obtained after mild acid hydrolysis of BSM Ac, acetyl; Lt, lactyl; Gc, glycolyl Methods 3.1 Release and Identification...
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Glycoprotein Methods and Protocols - P13
... (100 × cm) in a water jacket at 55°C and HPLC pump with refractive index and fluorescence detectors GlycoSepH™ and GlycoSepC™ HPLC column (Oxford GlycoSciences) Methods 3.1 Alkaline Borohydride Degradation ... 6.0, and load the sample carefully and directly to the center of the column, drop wise (see Note 3) Centrifuge for at 1100g and collect the excluded O-Glycanase O-Linked Chain Release and Fractionation ... (3 × 0.5 cm column) and elute with three column vol of HPLC-grade water Collect the effluent and eluent and then pool (2.5-mL volume) Load onto a PD10-Sephadex GM25 column and elute with HPLC-grade...
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Glycoprotein Methods and Protocols - P14
... was introduced in the 1970s and rapidly received a large application for analyzing the sequence of N-acetyllactosamine- and oligomannosidic-type glycans Originally, and before the development ... appearence of novel methods of ionization such as fast atom bombardment ionization (FAB), electrospray ionization (ESI), and matrixassisted laser desorption ionization (MALDI) Methods 2.1 Nuclear ... method was developed by Vliegenthart and colleagues during the 1970s and essentially applied to the structure determination of N-glycans of the Nacetyllactosamine and oligomannoside type (16) More...
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Glycoprotein Methods and Protocols - P15
... hygroscopic and must be thoroughly dried before weighing and making up as standards Standards of mM each of 2-, 3-, and 6-sulfo-Nacetylglucosamine in water may be aliquoted and stored at –20°C, and used ... including two external controls that contain an equimolar mixture of 2-, 3- and 6-sulfo-N-acetylglucosamine in water: a nmol control (1 nmol of each sugar sulfate), and a 15 nmol control (5 nmol of ... 212 Harrison and Packer Materials 2.1 Solvents and Reagents Water, carbonate-free (see Note 1), and sparged with a constant flow of argon 0.1 M NaOH, made up with decarbonated water, and similarly...
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Glycoprotein Methods and Protocols - P16
... Subheading 2., item 5e at 37°C and 5% CO2 Caco-2 and A431 cells are passaged (trypsinised) and split 1:4 and the medium is changed every to d LS174T cells are split 1:2 and the medium changed daily ... only MUC and MUC2 at early passage, but at later passage and in later premalignant and malignant stages, they show de novo expression of MUC5AC, MUC5B, and MUC6 (Corfield, Myerscough, and Paraskeva, ... clumps of cells and replate under standard culture conditions Reattachment of cells may take several days, and during medium changing, any floating clumps of cells must be centrifuged and replated...
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