use of the cytomegalovirus promoter for transient and stable transgene expression in mouse embryonic stem cells pdf
Báo cáo y học: "The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells" pot
... performed the experiments, analysed the data, and was responsible for writing the initial versions of the manuscript SA performed cell culture experiments MS and AT were involved in performing the ... very early in S-phase in wild-type ES cells (Figure 4), possibly reflecting a high demand for biosynthesis in these rapidly dividing cells Analysis of these three repeat sequences in the mutant ... retrotransposons (LINE and SINE B1) and rDNA repeats in wild-type OS25 ES cells and in mutant ES cells lacking Eed, Dnmt 1, Dnmt 3a/3b, G9a or Dicer The mean values and standard error of at least two independent...
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Identification of oct4 and sox2 targets in mouse embryonic stem cells
... Cooperative binding of Oct4 and Sox2 results in embryonic expression of the F-box containing protein 15 (Fbx15) and mutation of either binding site is known to abolish the activity of the enhancer ... Sox2 and Oct4 to the DNA via their respective DNA binding domains, and upon tethering of each factor to the enhancer region forming a ternary complex, new DNA-protein and protein-protein interactions ... connection between the intrinsic transcription factors and extrinsic signaling pathways in mouse embryonic stem cells (mESCs) Stat3 and Oct4 were shown to bind to the promoter of Nanog, and subsequent...
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Báo cáo y học: "Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells" pps
... conditions in mouse embryonic stem cells Methods Mouse embryonic stem cell culture The mouse embryonic stem (MES) cells (CCE-24) were routinely grown on 0.1% gelatin-coated dishes in Dubecco’s ... Page of 10 Figure The cDNA sequence of the truncated form of MsrA with the predicted open reading frame Thin lines indicate exon-exon junctions A The thick line points to the junction of exon and ... proteins with structure hindering the access for the long form protein, thus having an advantage to be evolutionarily selected and maintained Since the truncated protein itself does not contain the...
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The Use of Selected Medicinal Herbs for Chemoprevention and Treatment of Cancer, Parkinson’s Disease, Heart Disease, and Depression
... mediated through norepinephrine and the renin-angiotensin system originating in the kidneys (both neurohormonal deactivating effects), and the slowing of the heart rate and decreased nerve impulse ... an increase in the intracellular concentration of sodium and then an increase in the intracellular concentration of calcium (Sanborn, 2007) The increased myocardial 11 Use of Selected Medicinal ... injection of carrageenan For the abdominal constriction test, inhibition observed for the extract was almost as potent as that for indometacin In the formalin test, the V corymbosum extract and...
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Postal Savings and the Provision of Financial Services: Policy Issues and Asian Experiences in the Use of the Postal Infrastructure for Savings Mobilization pdf
... of home Internet and telephone banking services in place of postal counter services in Finland and other countries invite further scrutiny Although Internet usage in Finland is 43 per cent, the ... also increasing its independent branch network In 1987, in addition to the post offices, it had 50 branch offices in 33 cities and towns, 13 of them in the Helsinki area alone Following Finland’s ... likely because ING Barings is also the fifty per cent owner of the Netherlands Post With the Postbank and the Netherlands Post under the same ownership, the two institutions can again tap the synergies...
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Báo cáo khoa học: " Use of the n-butyl cyanoacrylate adhesive and the polyglactine thread suture for corneal rhaphy in rabbit (Oryctolagus cunicullus)" pptx
... observed, agreeing with the findings of Wilkie & Wolf [32] who used synthetic material in the repair of the cornea in dogs According to Morales et al [22], the corneal vascular neoformation appears ... allowing that the animals were maintained under anesthesia during a short period of time Similar results were obtained by Queiroz et al [26], when testing the adhesive in skin of cats During the ... extravasation of aqueous humor was observed in both the corneas submitted to the adhesive and the thread suture for the repair of the perforation Such fact was due to the efficient linkage of the adhesive...
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báo cáo khoa học:" Use of the intraosseous screw for unilateral upper molar distalization and found well balanced occlusion" pdf
... × 022 inch ss utility arch was used for protrusion of the upper incisors For retrusion of the mandibular incisors 016 × 022 inch continue arch with lingual root torque in incisor region and Class ... "U" bend touching the screw The TPA was soldered to the bands, the bands were cemented onto the premolars, and the U bend was bonded to the intraoral neck section of the screw using light-cured ... the use of a lot of appliances with the significant maxillary incisor proclination and increased in overjet at the end of the distalization [5,10,11] In the present study, extraction of the impacted...
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guide for the use of preplaced aggregate concrete for structural and mass concrete applications
... with the possible exception of some on admixture refinements Initially, in view of the lack of any performance history, the use of PA concrete was limited to the repair of bridges and tunnel linings ... strip of mortar in the joint The use of mastics that not harden has been found inadvisable because they tend to blow out as the grout rises behind the forms Forms constructed of tongue and groove ... reveal the weakening effect of excessive bleeding of the grout within the preplaced aggregate, nor does it account for the restraining effect on the expansion of the gas bubbles The next step is the...
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Tài liệu Báo cáo khoa học: "Demonstration of the UAM CorpusTool for text and image annotation" docx
... structural tagging, including co-reference linking, rhetorical structuring and syntactic structuring The use of the tool is rapidly spreading: in the first 15 months of availability, the tool has been ... images instead of text files In this context, one can swipe regions of the image to create a selection, and assign features to the selection Since stand-off annotation is used for both text and ... “containing” segments One can also search for segments containing a string Where a lexicon is provided (currently only English), users can search for segments containing lexical patterns, for instance,...
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How Canadians'''' Use of the Internet Affects Social Life and Civic Participation pdf
... continue to play a role in explaining some of the differences in the way the Internet is used in these areas Indeed, Canadians living in rural and remote areas report that the range of online ... amount of their time on the Internet engaging in activities involving social interaction in other forms Chart uses the 2005 GSS time use data to reveal the extent to which Internet users devoted their ... engaged in the Internet, and; senior citizens have become increasingly involved in using the Internet for communicating with family and socializing by playing games with others online The article...
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Báo cáo sinh học: " Generalizing the use of the canonical transformation for the solution of multivariate mixed model equations" doc
... following )f f linear system: = Now, premultiplying both sides by: and inserting: the system becomes: System [64] and has a is of the form solution equal to: Given the form of C and T and in T’C-’, ... corresponding to the fixed effects on the one hand and to the random on the other The off-diagonal blocks are moved to the right-hand side after the current solutions of the effects part, the system ... corresponding [12] as u C = W’y !4!: IB Q = Q9 S Q Q9( IN* ) and and define IN Premultiply both sides of the system by S-’ (S-’)’ and insert -’S * S in the right-hand side S S- in the left-hand side and...
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Báo cáo y học: "Clinical evaluation of the Life Support for Trauma and Transport (LSTAT™) platform Ken Johnson1, Frederick Pearce2, Dwayne" pps
... no other equipment were available Phase II: Evaluation of the LSTAT in a routine postoperative setting Nursing staff received a 45-minute training seminar in the use of the LSTAT, in which the ... which reported the patient’s vital signs for use during transport After having used the LSTAT in the recovery room, the nursing staff was asked to critique the use of the LSTAT in managing critical ... Technologies Inc, Sarasota, FL, USA) During two scenarios, the clinicians used the LSTAT and during the other two scenarios they used conventional equipment The clinicians were randomly assigned to one of...
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Analysis, evaluations and solutions for improving effectiveness of the foreign currency business operation of the Vietnam bank for Agriculture and rural development
... innovating financial, banking system in accordance with the international standard Vietnam is perform ing m easure to reorganize the financial system Vietnam government is interested in the innovation ... to prepare the necessary conditions to integrate in local and international community, in there have integration o f the banking-finance system Before international integration requesting, Vietnam ... borrowing demand o f the big projects increase quickly, especially foreign currency demand For this reason, any banks in Vietnam if promote and gain the initiative in the foreign currency business...
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Báo cáo lâm nghiệp:"An examination of the interaction between climate, soil and leaf area index in a Quercus ilex ecosystem" ppt
... 154 C Hoff and S Rambal fall and leaf quantity [33, 43] Important then are the timing of rainfall and drought events, the quantity of rainfall, the storage capacity of the soil and quantity and ... precipitation and evapotranspiration requirements, but also as a function of the retention properties of the soil and of the rooting depth of the vegetation The range of LAI obtained with varying water ... of balancing the benefits obtained by increasing LAI to capture radiation and the cost in terms of transpiration Haxeltine et al [26] limited the problem of optimization to the maximization of...
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báo cáo khoa học: "Development of PEGylated PLGA nanoparticle for controlled and sustained drug delivery in cystic fibrosis" pptx
... the high negative charge of the polymer and (b) forming a steric barrier against approaching opsonins and preventing agglomeration of nanoparticles [10] Therefore, by using a molecule like DSPE-mPEG2000 ... hypersecretion in these diseases In CF and COPD, the inflammatory response is neutrophilic and may be induced by infection or components in cigarette smoke Controlling inflammation is at the root of treatment ... Pa-LPS induced IL1-b and IL-6 levels (Fig 7), demonstrating the ability of PS-341 to refrain both basal and Pa-LPS induced inflammatory response (*p < 0.05) Since systemic administration of PS-341...
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Báo cáo y học: "Use of the probiotic Lactobacillus plantarum 299 to reduce pathogenic bacteria in the oropharynx of intubated patients: a randomised controlled open pilot study" pps
... and Probi AB Authors' contributions BK was the prime investigator and did most of the planning and performance of the study BK handled the primary data and did most of the statistical work, and ... effectiveness of oral Lp299 in reducing the incidence of VAP Materials and methods The study was approved by the Human Ethics Committee of Lund University and was performed in compliance with the Helsinki ... Good clinical practice and the International Conference on Harmonisation Guidance were applied and the investigation was carried out in the ICU of the Department of Anaesthesiology and Intensive...
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OCT 3 4 and SOX 2 are key factors for SDIA neurogenesis of mouse embryonic stem cells
... providing countless days of humor and inspiration, and providing invaluable thoughts on science and life, and all things in between Table of Contents Abstract Introduction Results Culturing of Mouse ... stromal-derived inducing activity (SDIA) model of neurogenesis, we investigated the effects of the targeted knockdown of Oct-3/4 and Sox-2 by short interfering RNAs (siRNAs) in mouse embryonic stem cells ... silencing of these binding targets, as a result of the combinatorial interactions of Oct-Sox-Nanog binding, has yet to be definitively shown (Remenyi et al., 2004) This thesis explores the roles of...
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Báo cáo khoa học: The relative contribution of mannose salvage pathways to glycosylation in PMI-deficient mouse embryonic fibroblast cells pdf
... determined the variation of amount of glycoprotein in cells As shown in Fig 5E, although the total amount of protein in cells decreased to 87% after 48 h of incubation, the amount of glycoprotein ... On the other hand, the amount of glycoprotein in the medium did not differ substantially from the beginning to the end of the experiment, because the medium was changed every h From these findings, ... culture and infection PMI-deficient mouse embryonic fibroblast cells (PMI-null cells) [18] and wild-type mouse embryonic fibroblast cells (WT cells) were grown in DMEM containing 20% fetal bovine serum...
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Báo cáo hóa học: " Hydrothermal Growth and Application of ZnO Nanowire Films with ZnO and TiO2 Buffer Layers in Dye-Sensitized Solar Cells" docx
... carrier recombination [10] However, by introducing a blocking layer at the base of the ZnO films, the in uence of the blocking layer on the performance of ZnO DSSCs is an ongoing debate [10] In this ... 0.86–1.02% by introducing the TiO2 blocking layer The increase in Isc can be attributed to the increase in the aspect ratio of the nanowire arrays with and without the TiO2 blocking layer from ... layer (*0.8–1 lm thick) for spincoating, resulting in the maximum loss of Voc (about 40 mV) In order to further investigate the effect of a TiO2 buffer layer on the performance of ZnO nanowire-based...
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