use of chemical mutagenesis in mouse embryonic stem cells pdf

Báo cáo y học: "The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells" pot

Ngày tải lên : 14/08/2014, 08:20

... was used to analyze the impact of different chromatin modifiers on the replication timing profile of mouse ES cells We show that, while early replication in ES cells correlates with peaks of increased ... fibroblasts remained after 20-25 minutes plating in non-gelatinized plates Pre-plating of feeder-dependent ES cells in this way may result in a slight delay in the apparent time of replication ... replication in ES cells is altered by mutation of chromatin modifiers Satellite Satellite replication in ES cells is altered by mutation of chromatin modifiers (a) Summary of replication timing of repeat...

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Báo cáo y học: "Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells" pps

Ngày tải lên : 10/08/2014, 05:21

... conditions in mouse embryonic stem cells Methods Mouse embryonic stem cell culture The mouse embryonic stem (MES) cells (CCE-24) were routinely grown on 0.1% gelatin-coated dishes in Dubecco’s Modified ... thus producing a shorter isoform with a presumably truncated protein product containing the conserved catalytic active site in cultured mouse embryonic stem cells Due to the importance of anti-oxidative ... Anoxia/reoxygenation treatment The anoxic treatment of mouse embryonic stem cells was performed by incubating the cells in an anaerobic chamber (Sheldon Manufacturing Inc., Cornelius, OR) supplied with 90%...

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Identification of oct4 and sox2 targets in mouse embryonic stem cells

Ngày tải lên : 11/09/2015, 16:03

... Genome-wide Mapping of Oct4-DNA Interactions in Mouse Embryonic Stem Cells 4.1 Introduction 74 4.2 Results 76 4.2.1 Optimisation of large-scale ChIP 76 4.2.2 Global mapping of Oct4 binding sites in mESCs ... Genome-wide Identification of Sox2-DNA Interactions in Mouse Embryonic Stem Cells 5.1 Introduction 95 5.2 Results 96 5.2.1 Optimisation of ChIP and global mapping of Sox2 binding sites in mESCs 96 5.2.2 ... Function of genes associated with the binding of Oct4, Sox2, Stat3 and Smad1 in mouse embryonic stem cells as annotated in NCBI xiii LIST OF FIGURES Figure 1.1 A schematic view of mouse pre-implantation...

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Dissecting transcriptional network in mouse embryonic stem cells

Ngày tải lên : 10/09/2015, 15:51

... comprised of a mixture of a large population of undifferentiated cells and differentiated cells of multiple lineages EC cells could be maintained indefinitely with mitotically inactivated embryonic ... expression of E-cadherin, suggesting that these cells are in a latent state of pluripotency Figure 1.1 Origin of stem cells during mammalian embryogenesis In this figure, the pluripotent cells of the ... regulation of these cells, to understand the molecular mechanisms underlying pluripotency 1.2 Characteristics of mouse embryonic stem (ES) cells Mouse ES cells are well known for two distinguished...

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Báo cáo khoa học: The relative contribution of mannose salvage pathways to glycosylation in PMI-deﬁcient mouse embryonic ﬁbroblast cells pdf

Ngày tải lên : 30/03/2014, 04:20

... amount of glycoprotein in cells As shown in Fig 5E, although the total amount of protein in cells decreased to 87% after 48 h of incubation, the amount of glycoprotein decreased more The results of ... [18] and wild-type mouse embryonic ﬁbroblast cells (WT cells) were grown in DMEM containing 20% fetal bovine serum CDG-Ib patient cells were grown in a-MEM containing 10% fetal bovine serum All media ... with 200 lL of lipofectamine solution, dissolving lL of Lipofectamine 2000 in Opti-MEM, and transfected into subconﬂuent 293A cells in mL of Opti-MEM in six-well plates The 293A cells were then...

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Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf

Ngày tải lên : 07/08/2014, 20:23

... We determined the H2O2-induced ROS generation in mouse -5 embryonic stem cells using DCF-DA (10 M), a fluorescent probe of intracellular H2O2 Production of ROS was -3 detected in the cells exposed ... Min JJ, Park KS High glucose-induced inhibition of 2-deoxyglucose uptake is mediated by cAMP, protein kinase C, oxidative stress and mitogen-activated protein kinases in mouse embryonic stem cells ... pretreatment with DHT in the mouse ES cells (Figs 5A, B, C and D) In addition, as shown in Fig 5E, the maximum decrease in the level of [ H] thymidine incorporation was observed following 24 h of H2O2 exposure...

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Báo cáo y học: "A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells" doc

Ngày tải lên : 14/08/2014, 20:22

... murine ecotropic leukemia viruses by an expression cloning strategy in fibroblasts [17] In our study we used a loss -of- function assay, based on the principle of negative selection of infected cells ... ES cells after a single round of infection with 0.1 ml viral supernatant are illustrated by staining puromycin-resistant colonies in 24-well plates Figure (see following page) Super-infection of ... effect of inhibitors will be rapidly selected, diminishing the effectiveness of antiviral agents The main drugs currently used to treat HIV infection are inhibitors of two viral proteins, namely...

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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives

Ngày tải lên : 11/09/2015, 10:14

... 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term ... Expression of Shh during development Whole-mount in- situ hybridization of Shh in E9.5 days post coitum mouse embryo showing (A) the cross section of the spinal cord (dotted line ’ in B) showing Shh ... Miss Tan Ker Sin from the Stem Cell Group ,Bioprocessing Technology Institute, assisted in the cloning of all plasmids 3.1.2 Plasmids CMV expression vectors containing mouse Gli1, mouse Gli2 and...

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OCT 3 4 and SOX 2 are key factors for SDIA neurogenesis of mouse embryonic stem cells

Ngày tải lên : 27/11/2015, 11:25

... life, and all things in between Table of Contents Abstract Introduction Results Culturing of Mouse Embryonic Stem Cells (mESC) a Culturing Conditions b Expression of Oct-3/4, Alkaline Phosphatase ... and Vimentin expressions in SDIA cultures List of Illustrations Illustration – Invitrogen BlockIT tetracycline inducible short, hairpin RNA system Introduction Mouse embryonic stem cells (mESC) ... Utilizing a stromal-derived inducing activity (SDIA) model of neurogenesis, we investigated the effects of the targeted knockdown of Oct-3/4 and Sox-2 by short interfering RNAs (siRNAs) in mouse embryonic...

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Báo cáo sinh học: "Compound developmental eye disorders following inactivation of TGF signaling in neural-crest stem cells" doc

Ngày tải lên : 06/08/2014, 18:21

... and survival of late-born retinal ganglion cells in the developing mouse retina Development 2005, 132:311-322 23 Hjalt TA, Semina EV, Amendt BA, Murray JC: The Pitx2 protein in mouse development ... anomalies in Tgfbr2-mutant mice (a) Toluidine blue staining of semi-thin sagittal sections of eyes at E18 reveals a smaller size with no anterior chamber and an infiltration of cells behind the lens in ... mapping of ocular NC-derived cells in vivo The Rosa26 reporter (Rosa26R) mouse strain expresses ␤-galactosidase following Cre-mediated recombination [41] To define the distinct contribution of...

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báo cáo khoa học: " Microarray-based analysis of microRNA expression in breast cancer stem cells" doc

Ngày tải lên : 10/08/2014, 10:20

... other stem cells, indicating that these miRNAs may play a constitutive role in maintaining the biological characteristics of stem cells [40,41] Future work should include verification of the ... staining and immunohistochemical staining The tumors in the BCSCs group were positive for both EMA and SMA, indicating that they were included of both luminal epithelial and myoepithelial cells ... profiles by microarray with Q-RTPCR verification Haematoxylin and eosin (H&E) staining and immunohistochemical staining are shown on pathology sections of tumors implanted in NOD/SCID mice In...

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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx

Ngày tải lên : 18/06/2014, 15:20

... Translational Medicine 2008, 6:54 cells, the number (cells/ mm2 /mouse) of single stranded DNA (ss-DNA)+ cells in one field in the ischemic core from each mouse in the saline- or hES-ECs+MCs-injected group ... the saline- or hMNCs-injected groups, CBF in the hES-ECinjected group increased significantly, while no significant increase in the number of mouse PECAM-1 positive cells was observed in the ... human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of VEGF-R2, VE-cadherin and TRA-1 during the...

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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc

Ngày tải lên : 18/06/2014, 15:20

... expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted by a specific miRNA is a major obstacle in understanding the miRNA ... expression profiling of single whole embryonic stem cells Nucleic Acids Res 2006, 34(2):e9 Chen C, Ridzon D, Lee CT, Blake J, Sun Y, Strauss WM: Defining embryonic stem cell identity using differentiation-related ... names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture...

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Báo cáo y học: "A mouse embryonic stem cell bank for inducible overexpression of human chromosome 21 genes." ppsx

Ngày tải lên : 09/08/2014, 20:22

... to increased dosage of a gene and the degree of a certain property of the encoded protein, called intrinsic disorder [25] The protein disorder is defined as the total number of amino acids included ... Average induction of the 32 inducible clones by q-PCR Baseline expression (0 hours of induction - white bars), following induction of transgene (after 24 to 48 hours of growth in medium deprived of ... consequence of a deficit in tumour angiogenesis arising from suppression of the calcineurin pathway [12] Overexpression of a number of HSA21 genes, including Dyrk1a, Synj1 and Sim2, results in learning...

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Báo cáo y học: " Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development" ppt

Ngày tải lên : 09/08/2014, 20:22

... interactions where both interacting proteins in human had exactly one corresponding protein in mouse The PPIs in our clusters were extracted Page 13 of 16 with the GraphWeb tool [56] by setting ... propagated indefinitely in vitro in the presence of leukemia inhibitory factor (LIF) [6,7] When transplanted into a mouse blastocyst, mESCs integrate into the embryo and contribute to all cell lineages, ... steps in mESCs, we used a combination of computational and experimental approaches Analysis of the expression of TFs associated with mESC adipogenesis during mouse embryogenesis and in mouse adipose...

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báo cáo khoa học:" Induction of osteogenic markers in differentially treated cultures of embryonic stem cells" ppt

Ngày tải lên : 12/08/2014, 01:22

... xenogenic cells [13-16] There are some reports that use totipotential embryonic stem cells in tissue engineering of bone [17,18] Embryonic stem cells (ESCs) are routinely derived from the inner cell ... are von Kossa stainings with arrows pointing to the deposition of calcium phosphate-containing minerals that stained in black Figure LIF, stem cell PCR showing amplification of the without hematopoeticqualitative ... basis of pluripotency in mouse embryonic stem cells Cloning Stem Cells 2004, 6(4):386-391 Smith AG, Heath JK, Donaldson DD, Wong GG, Moreau J, Stahl M, Rogers D: Inhibition of pluripotential embryonic...

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Báo cáo y học: " Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy" pps

Ngày tải lên : 13/08/2014, 09:21

... embryonic stem cells using lentiviral vectors Stem cells 2003, 21:111-117 Gropp M, Itsykson P, Singer O, Ben-Hur T, Reinhartz E, Galun E, Reubinoff BE: Stable genetic modification of human embryonic stem ... Cytokines and BMP-4 promote hematopoietic differentiation ofhuman embryonic stem cells Blood 2003, 102:906-915 Wang L, Li L, Menendez P, Cerdan C, Bhatia M: Human embryonic stem cells maintained in ... mainly targets cells of the hematopoietic system, namely, T cells, macrophages, and dendritic cells [15] As infection progresses, the immune system is rendered defenseless against other invading...

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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx

Ngày tải lên : 13/08/2014, 23:20

... The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics ... Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent with the President's belief in the fundamental value and sanctity of human life The ... acids, nucleosides, antibiotics, L-glutamine with 2000 IU/ ml mouse recombinant leukemia inhibiting factor in 6% CO2 at 37°C Fully-expanded or hatching mouse blastocysts were plated as controls...

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Báo cáo y học: "nalysis of the mouse embryonic stem cell regulatory networks obtained by ChIP-chip and ChIP-PET" pptx

Ngày tải lên : 14/08/2014, 20:22

... different batches of serum used to culture these cells Finally, different binding results may be obtained due to differences in ES cell strains Therefore, with the availability of binding information ... targets in the ChIP-PET data can, at least in part, be explained by a lack of depth in sequencing Differentially regulated targets of OCT4 and NANOG Since protein-DNA binding alone is not indicative ... can be used to obtain more detailed binding data on a genome-wide scale Additionally, the finding that approximately 40% of the binding sites were found in intragenic regions is also in concordance...

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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1

Ngày tải lên : 09/09/2015, 17:54

... dissociating into single cells following incubation with Accutase for minutes, collecting cells by centrifugation at 1000 rpm for minutes and replating at a ratio of 1:6 3.6.2 Differentiation of ReN-VM cells ... passaging of the hESCs For transfection of cells in one well of a six-well plate, 10 µg of plasmids was complexed with 10 µl of Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ... differentiation of pluripotent stem cells into neuroepithelial stem cells and radial glia 15 Figure 1.5 Correlation of expression profiles of lncRNAs with protein-coding gene markers during embryoid...

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