unipotent primordial germ cells
Cell Cycle Regulation in Mammalian Germ Cells
... survival and has a role in maintenance of reproductive germ cells The knockout leads to progressiveloss of germ cells, Sertoli cells and Leydig cells and cause extensive testicular degradation A ... sperm cells and oocytes from ES cells will be useful to study early meiosis events (Hubner et al 2003) Additionally, technology for deriving germ cells from human ES cells will permit studies of germ ... 29:49–53 McLaren A (2003) Primordial germ cells in the mouse Dev Biol 262:1–15 Mintz B, Russell ES (1957) Gene-induced embryological modifications of primordial germ cells in the mouse J Exp Zool...
- 31
- 377
- 0
Báo cáo sinh học: "TBP2 is a general transcription factor specialized for female germ cells" doc
... show that Xenopus oocytes lack TBP protein, whereas TBP2 is the major TBP-type factor in the germ cells Later, in eggs and early embryos, TBP2 levels decrease, whereas TBP starts to accumulate ... regula Oogenesis Meiotic maturation Fertilized egg/ zygote Zygotic gene expression Frog 4000 cells Mouse cells Figure Regulation of TBP and TBP2 during oogenesis and the early stages of embryogenesis ... J: Developmental and cell type-specific regulation of core promoter transcription factors in germ cells of frogs and mice Gene Expr Patterns 2006, 6: 409-419 10 Gazdag E, Rajkovic A, Torres-Padilla...
- 4
- 235
- 0
Báo cáo y học: "On the number of founding germ cells in humans" ppt
... only in germ cells of the mouse, but also in somatic cells that surround these germ cells [21] Recent description of cytochemical markers that are more specifically restricted to the germ cells ... A germ- cell odyssey: fate, survival, migration, stem cells and differentiation Meeting on germ cells EMBO Rep 2003, 4:352-7 Lawson KA, Hage WJ: Clonal analysis of the origin of primordial germ ... of and weeks Using morphological clues to distinguish germ cells from somatic cells, these authors were able to establish the number of germ cells per tissue sample throughout this period We used...
- 6
- 336
- 0
Báo cáo y học: "Enigma variations: control of sexual fate in nematode germ cells" docx
... focused on the germline, and the role of the three fem genes, which are required for spermatogenesis in both sexes of C elegans Nucleus Cytoplasm Figure Two routes to the nucleus in the germline cell-fate ... bind directly to TRA-1 [14,15] Furthermore, mutations that disrupt this interaction cause all germ cells to choose oogenesis [14-17] These findings raise several questions Why are there two information ... regulates transcription of fog-3, which controls germ cell fate in C elegans Development 2000, 127:3119-3129 Chen PJ, Cho S, Jin SW, Ellis RE: Specification of germ cell fates by fog-3 has been conserved...
- 3
- 185
- 0
Interactions between early germ cells and inner germarial sheath cells in drosphila ovary
... between early germ cells and inner germarial sheath cells (IGS cells) during Drosophila oogenesis Two aspects of signals are studied: one, signals from early germ cells to IGS cells and the other, ... early germ cells and IGS cells in adult Drosophila germaria As such, two aspects will be investigated as listed below: I) signals from early germ cells to IGS cells; II) signals from IGS cells ... investigate the signals from IGS cells to early germ cells (Chapter IV) Progress related to germline stem cells (GSCs), the GSC niche and essential factors in Drosophila germaria is reviewed in Chapter...
- 145
- 627
- 0
Wheat germ agglutinin conjugated poly dl lactic co glycolic acid (PLGA) nanoparticles for enhanced uptake and retention of paclitaxel by colon cancer cells
... Caco-2 cells; (b) HT-29 cells; (c) CCD-18Co cells (Mean ± SD, n = 6) 116 4.9 In vitro cytotoxicity profiles of P/CreEL, , PNP and WNP against (a) Caco-2 cells; (b) HT-29 cells; (c) CCD-18Co cells ... against colon cells (a) positive and negative control; (b) Caco-2 cells; (c) HT-29 cells; (d) CCD-18Co cells (Mean ± SC, n = 6) 111 4.5 Uptake of fWN by Caco-2, HT-29 and CCD-18Co cells as a function ... paclitaxel to colonic cancer cells Glycosylation patterns of representative colon cancer cells (Caco-2 and HT-29 cells) and normal cells (colon fibroblasts, CCD-18Co cells) were first investigated...
- 231
- 349
- 0
Báo cáo y học: " Effect of Weight Reduction on Cardiovascular Risk Factors and CD34-positive Cells in Circulatio"
... progenitor cells (CD34+/CD45- cells) These cells are thought to be early precursors of endothelial progenitor cells and function to replenish aging as well as damaged endothelial cells that line ... progenitor cells Our decision to considered CD34 positive/CD45 negative circulating cells as “circulating EPCs” was based on the work [28], in which blood–derived cells from which endothelial cells ... culture were developed were described as cells expressing CD34 antigen It has been hypothesized that endothelial progenitor cells and hematopoietic progenitor cells have common precursor, the hemangioblast...
- 8
- 594
- 0
Báo cáo y học: "Positioning Effects of KillerRed inside of Cells correlate with DNA Strand Breaks after Activation with Visible Light"
... For comparison cells were transfected with pH2A-KillerRed whose histone fusion partner H2A acts as a structural protein, a crucial component of the chromosomal chromatin In the cells the histone ... could demonstrate that a very close distance of KillerRed to DNA did not allow the cells to survive The investigated cells were grown and treated in cell culture, but illumination and the following ... cancer cells, first characterized and documented by the Stone group [19], were cultivated and maintained in RPMI1640 (Gibco 11825) amended with FCS (2%) (Gibco) For experiments subconfluent cells...
- 9
- 390
- 0
Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
... subjected to SDS–PAGE, and bands were captured Treatments of AGS cells AGS cells in the logarithmic phase were divided into two groups Cells in one group were grown in the medium containing μmol/L ... 24 h Detection of ROS using Flow cytometry Cells were washed with PBS once After trypsin digestion, AGS cells were re-suspended in PBS and 1×104 viable cells were measured in each sample by FACScalibur ... AGS cells after24 h of stimulation by Hp extracts of various concentrations a: Negative control Cells were stimulated by 960 µg/ml E coli extract and the ROS level was 3; b: Positive control Cells...
- 12
- 557
- 2
Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
... molecules were involved Once tissues were ischemic, stem cells in circulation are adherent to the vascular endothelial cells, cross the endothelial cells, migrate and finally reached at ischemic sites ... of stem cells, it is very important to observe the survival and curative effects of transplanted stem cells Traditional immunohistochemical method can easily identify the transplanted cells with ... stromal progenitor cells (mesenchymal progenitor cells) : implications for therapeutic use Bone Marrow Transplant,1995,16(4):557-564 Aggarwal S, Pittenger MF Human mesenchymal stem cells modulate...
- 10
- 584
- 0
Báo cáo y học: "Association between regulated upon activation, normal T cells expressed and secreted (RANTES) -28C/G polymorphism and asthma risk – A Meta-Analysis"
... cell types present in asthmatic airways, such as T cells, platelets, macrophages, endothelial cells, fibroblasts, epithelial cells, and mast cells, have the capacity to generate RANTES [8], which ... chemokine that has been shown to be a potent chemoattractant for T cells, eosinophils, basophils, monocyte/macrophages, and mast cells [22] It has been shown that RANTES induces recruitment of eosinophils ... Hizawa et al [14] suggested that mononuclear cells from subjects who carried the -28G allele produced significantly greater levels of RANTES protein than cells from subjects who did not carry this...
- 7
- 525
- 0
Báo cáo y học: "High-Resolution Flow Cytometry: a Suitable Tool for Monitoring Aneuploid Prostate Cancer Cells after TMZ and TMZ-BioShuttle Treatment"
... realizing high local TMZ concentrations in the nuclei of target cells To eradicate the target cells, without considering the HRPC cells resistance against therapeutic interventions it is necessary ... (Partec, Munster/Germany) equipped with mercury lamp 100 W and filter combination for 2, 4-diamidino-2-phenylindole (DAPI) stained single cells From native sampled probes the cells were isolated ... DU 145 prostate cancer cells after treatment with TMZ alone and with its TMZ-BioShuttle derivative Control DU 145 cells form a continuous monolayer, while treated DU 145 cells show loss of adhesion...
- 10
- 408
- 0
Báo cáo y học: "Why are some children with early onset of asthma getting better over the years" -aneuploid-prostate-cancer-cells-after-tmz-tmz-bioshuttle-treatment.html#post143974
... international Study of Asthma and Allergies in Childhood (ISAAC): objectives and methods; results from german ISAAC centres concerning traffic density and wheezing and allergic rhinitis Toxicol Lett...
- 10
- 456
- 0
Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... of exposure to hypoxia or normoxia, cells (1×106 cells/ flask) were collected by trypsinisation Cell lysates of hypoxia-treated cells and untreated control cells were assayed for phosphorylated ... JNK1&2 (-fold of control) = absorbance of hypoxia-treated cells/ absorbance of control cells RT-PCR analysis Total RNA was extracted from JAR cells using RNeasy mini kits (QIAGEN, Mississauga, Canada) ... time-dependent manner for JAR cells In cells transfected with Bag-1 Morpho/AS, significantly more internucleosomal DNA fragmentation was detected than in non-treated control JAR cells after hypoxia treatment,...
- 9
- 499
- 0
Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"
... in confluent HepG2 cells in vitro [21], the present study was performed only using HepG2 cells HepG2 cells were seeded in 250 ml tissue culture flasks (Falcon, Heidelberg, Germany) at 1x105/ml ... medium (Gibco, Eggersheim, Germany) supplemented with 10% fetal bovine serum (Boehringer Mannheim, Germany), 100 U/ml penicillin and 100 µg/ml streptomycin (ICN, Meckenheim, Germany) at 37°C in a ... group 1, HepG2 treated cells with mM ethanol for h; and group 2, HepG2 cells without ethanol exposure used as a control All chemicals were purchased from Sigma Aldrich (Seelze, Germany) Both the ethanol...
- 8
- 702
- 0
Báo cáo y học: " Low concentration of ethanol induce apoptosis in HepG2 cells: role of various signal transduction pathways"
... Meckenheim, Germany) at 37°C in a humidified atmosphere of 7.5% CO2 After days of cell culture, the cells were harvested with 0.05% trypsin / 0.02% EDTA (Gibco BRL, Eggersheim, Germany) Cells were ... Human hepatocellular carcinoma (HepG2) cells HepG2 cells, derived from human hepatocellular carcinoma (obtained from Deutsches Krebsforschungszentrum Heidelberg, Germany) were seeded in 250 ml tissue ... Heidelberg, Germany) at concentrations of 1x105/ml Six sets of experiments were performed (n=6) Each set consist of two groups as follow: HepG2 cells treated with mM ethanol and HepG2 cells without...
- 8
- 510
- 0
Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"
... in the Department of Biochemistry of the Charité, Berlin, Germany using the ABI PRISM Big Dye Terminator system (Applied Biosystems, Germany) Sequences were analyzed using the Chromas software ... agarose gel and subsequently purified with the QIAquick PCR purification system 149 (QIAGEN, Hilden, Germany) Figure (A) Overview depicting the common exons – 11 and the three transcript variants (TV)...
- 4
- 393
- 0
Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... some cells detached after exposure to low temperature Virtually all of the detached cells were determined to be non-viable with tryphan blue staining (data not shown) All of the detached cells ... Altussheim, Germany) Additionally, the mFISH assay [10] was used to screen for the presence of chromosomal translocations Chromosome paints were obtained from MetaSystems GmbH (Altlussheim, Germany) ... so as to enable some of the cells to undergo mitosis Mitotic hESC were then arrested at metaphase by colcemid treatment (0.1 μg/ml) for 16-18 h Subsequently, the cells were subjected to hypotonic...
- 6
- 477
- 0
Báo cáo y học: "ene expression analysis of human red blood cells"
... fraction tested by independent methods blood cell count RBCs (n=5) (cells/ µL) cytometer white blood cells platelets (cells/ µL) (cells/ µL) 6600000±2000 n.d.* n.d.* FACS 6300000±1520 n.d.* n.d.* blood ... by Pappenheim staining of blood slides, flow cytometry (MÖLAB, Germany) and FACS analysis (Cytomics FC 500 CXP, Beckman Coulter, Germany) using labeling with human leucocyte- (CD45) or platelet ... Total RNA from RBCs was purified using reagents provided in the PAXgene Blood RNA Kit (Qiagen, Germany) and tested with RNA LabChip Kit by 2100 Bioanalyzer (Agilent, USA) cDNA was synthesised...
- 4
- 398
- 0
Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
... expression, we transfected pDsRedCLDN1-NLS into G361 cells, and then treated the transfected cells with phorbol ester (PMA) Treatment of cells with 200nM PMA resulted in an almost complete exclusion ... nucleus of melanoma cells, so cells were stained with an antibody to pan-PO4-PKC Confocal microscopy demonstrates that active PKC can be found in the nucleus of G361 melanoma cells D) To determine ... exist in the nucleus of melanoma cells, and the presence of active PKC isoforms in the nuclei of many cell types has been confirmed (10-12) Staining of the G361 cells with antibodies to phosphorylated...
- 9
- 592
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008