... live in a snake.14. My brother managed to kill the snake just at the time when I were almost exhausted. Supply thecorrectformofthe verbs1. Cats could fly if they (have) wings.2. If Peter ... (not,want) to live in a snake.14. My brother managed to kill the snake just at the time when I (be) almost exhausted. Ifhe (be) a little late, I (kill) by the snake.15. Had I know you were ill, ... snake.14. My brother managed to kill the snake just at the time when I (be) had been almost exhausted. If he (be) had been a little late, I (kill) would have been killed by the snake.15. Had...
... Give thecorrectformofthe verbs (Englsh 8)1 .The sun always (rise) in the east. Look, it (rise)!2. I don’t think I (go) out tonight. ... her homework yet.28. Would you like some coffee? I just (make) some.29. She (feed) the cat yet? - Yes, she (feed) it half an hour ago.30. The weather (become) colder and colder in December.31. ... evening. I (stay) at home.33. The film (begin) at 7.30 or 8.00?34. You (be) away? - Yes. I (go) to the country last Sunday.35. They just (phone) to say that they (not come) back till Friday...
... biological function ofthe pro -form of bone morphogenetic protein-2 (BMP-2), a member ofthe transforminggrowth factor b (TGFb) ⁄ BLP superfamily. The role ofthe 263 amino acids of the pro-peptide ... Furthermore, the pro-peptide of GDF-8 impairs interaction ofthe mature part withits receptors [16]. In the case of BMP-9, the pro-pep-tide appears not to alter significantly the activity of the mature ... interested in the role ofthe 263amino acid pro-peptide of BMP-2, because evidenceaccumulated over recent years has shown that the pro-forms of growth factors can modulate the activities of the mature...
... rates) prior to and after the transition ofthe enzyme to amore active form, respectively, t is the time and s is the lag-time. The rate constant, k, for the activation ofthe enzyme isobtained ... lip prevented binding ofthe C-terminalresidues over the active site, or the absence of the C-terminal residues caused the movement ofthe lip(Fig. 1B). In addition, the Cachain between aminoacid ... between the dCTP(dUTP)-bindingconformer and the dTTP-binding conformer of dCTPdeaminase. We were not able to identify structuralchanges in the main chain ofthe subunit, or in the interaction of...
... the SH3 domain of Noxo1 participates inregulation of Nox3, the role ofthe PX domain in Nox3activity remains unknown.In the process of cloning of human Noxo1, somespliced transcripts ofthe ... To compare the amounts of the two variants expressed in the testis, we performed the PCR using the primers ‘d’ and ‘c’, where the cDNAs for both Noxo1b and Noxo1c are amplified as the insert region ... between the pair of primers(Fig. 2A). The lengths ofthe PCR products of Noxo1band Noxo1c were 225 and 240 bp, respectively. Todelineate the difference in the two products, we subjec-ted the...
... enzyme. The catalytic mechanism of hydrolysis The fructose present in the crystal structure of the complex is the result of hydrolysis of raffinose. The conformation ofthe sugar observed in the electrondensity ... attack on the anomeric carbon of fructose. The leaving group iscarboxylate of Asp54.Dimerization The asymmetric units ofthe crystals of both the apoand complexed formofthe enzyme consist of dimerswith ... blades in the axisarea are of polar character. The water channel in the apo formofthe enzyme runs along the axis of the b-propeller through the whole domain and is onlyblocked by Cys236 in the...
... between the hypophosphorylated initiation isoform IIA and the hyperphosphorylated elongating isoform IIO thatexhibits reduced electrophoretic mobility comparedwith the IIA isoform. Whereas the ... replicases in the eukary-otic cell [22]. Therefore, we tested the effect of the replicative state ofthe cell on the interaction betweenPol e and RNA pol II. A possible confinement of the interaction ... specifically in the G1phase of the cell cycle. This effect could be attributed to inhi-bition of transcription of cell cycle-regulated genes atthis phase ofthe cell cycle [51]. Nevertheless, it...
... regulation ofthe PKIand PKI isoforms ofthe inhibitor protein ofthe cAMP-dependentprotein kinase. J. Biol. Chem. 272, 20011–20020.20. Byler, D.M. & Susi, H. (1986) Examination ofthe secondarystructure ... Characterization of PKIgamma, a novel isoform ofthe protein kinase inhibitor of cAMP-dependent protein kinase. J. Biol. Chem. 272, 18169–18178.4. Gamm, D.M. & Uhler, M.D. (1995) Isoform-specific ... showedasinglebandbySDS/PAGE(Fig. 2). The assay of its activity demonstrated that the purified PKIb inhibited the catalytic subunit of cAPK with the specific activity of 6.0 · 104unitÆmg)1(Fig. 3A) .The Kivalue determined...
... effect on the thermalunfolding of proCPY. The Pmvalue for CPY was higher than that of itsunglycosylated form, though the DV and DGPvalues werealmost the same. The Pmvalue of proCPY was ... the corresponding values of proCPY and Dgly proCPY (Fig. 2B), indicating that the precursor form was less thermally stable than the mature form, regardless ofthe carbohydrate moiety.After the ... unfolding ofthe unglycosylated form. At high pressure, in the glycosylated forms the carbohy-drate moiety of CPY and proCPY would be hydrated tocompensate the volume contraction and the protein...
... iden-tified as the major and the minor forms ofthe holo-ACP structure, on the basis of their percentage contri-butions (65% and 35%, respectively) to the overallstructure ofthe protein. The structures ... both forms have the same amino acidsequence, and they have similar secondary structures, it was found that the – DG of unfolding ofthe holo form was lower than that ofthe apo form atall the ... unfoldingthermodynamic parameters ofthe two forms are givenin Tables 1–3. In the entire experimental regime, it isseen that the holo form presents better stability than the other form (Fig. 4C). The...