ultraviolet induced apoptosis in embryonic stem cells in vitro pdf
Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... analyzed forty proteins by using the PowerBlot (BD Bioscience Pharmingen, San Diego, CA, USA) system We selected 40 apoptosis- related proteins to examine the mechanism of hypoxia -induced apoptosis After ... global changes in the proteins related to apoptosis during hypoxia to clarify the mechanism of hypoxia -induced apoptosis, and to find out useful hypoxia-related markers of trophoblastic cells Materials ... (data not shown) Int J Med Sci 2007, 39 Hypoxia -induced apoptosis in the JAR cell line, and Bag-1 Morpho/AS enhanced apoptosis of the cell line With hypoxic treatment for 24 hours, internucleosomal...
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Báo cáo Y học: Requirement of caspase and p38MAPK activation in zinc-induced apoptosis in human leukemia HL-60 cells potx
... for Zn -induced apoptosis in human Burkitt lymphoma B cells An increase in labile Zn in the cytoplasm may suppress apoptosis by inhibition of the action of caspase, cytoplasmic apoptosis inducer, ... protein)1, indicating that the ability of Zn to induce apoptosis is dependent on the intracellular Zn level In fact, it has been shown that treatment with Zn (80–200 lM) induced apoptosis in mouse ... aspects in the regulation of apoptosis is the involvement of mitogen-activated protein kinases (MAPKs), a family of serine/threonine kinases that mediate intracellular signal transduction in response...
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Báo cáo Y học: DAP kinase activity is critical for C2-ceramide-induced apoptosis in PC12 cells ppt
... kinase activity is critical for C2- and C8-ceramideinduced apoptosis in PC12 cells DISCUSSION DAP kinase is a Ca2+/calmodulin-regulated serine/threonine kinase that participates in apoptosis induced ... calmodulin-binding domain In the K42A-DK mutant, a lysine residue at position 42 in the kinase domain (asterisk) is replaced by an alanine The DD-DK construct contains the death domain and the serine-rich ... mediator of apoptosis [1±3] As Western blotting showed that DAP kinase was endogenously expressed in PC12 cells (Fig 2, lane 1), we examined whether the ceramide -induced apoptosis in PC12 cells was...
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Báo cáo khoa học: BRCA1 accumulates in the nucleus in response to hypoxia and TRAIL and enhances TRAIL-induced apoptosis in breast cancer cells pdf
... proteins involved in various cellular processes, including inhibition of apoptosis Hypoxia has been reported to inhibit apoptosis induced by tumor necrosis factor-related apoptosisinducing ligand ... hypoxia in MDA-MB-468 cells (Fig 2B), resulting in a 35% decrease in nuclear BRCA1 relative to the level in normoxic cells in the presence of 2E5 The decrease in C Fig Hypoxia -induced changes in BRCA1 ... the data in Fig support the involvement of a TRAIL-dependent process in mediating hypoxiainduced changes in BRCA1 localization TRAIL, but not hypoxia, induces apoptosis in breast cancer cells As...
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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of VEGF-R2, VE-cadherin and TRA-1 during the ... Translational Medicine 2008, 6:54 cells, the number (cells/ mm2/mouse) of single stranded DNA (ss-DNA)+ cells in one field in the ischemic core from each mouse in the saline- or hES-ECs+MCs-injected group ... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs),...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are ... expression profiling of single whole embryonic stem cells Nucleic Acids Res 2006, 34(2):e9 Chen C, Ridzon D, Lee CT, Blake J, Sun Y, Strauss WM: Defining embryonic stem cell identity using differentiation-related ... detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in two types of adult cells HMVEC and HUVEC Differences in the...
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Báo cáo khoa học: " Cap-independent protein translation is initially responsible for 4-(N-methylnitrosamino)-1-(3-pyridyl)-butanone (NNK)-induced apoptosis in normal human bronchial pithelial cells" pot
... Our findings of rapamycininduced caspase activation is coincident with Nottingham et al’s [28] result that rapamycin expressed activated caspase in spinal cord of rats Rapamycin-dependent increase ... gamma-tocopherol quinone induced apoptosis in cancer cells through caspase activation and cytochrome c release [5] In FACS analysis, NNK induced significant apoptosis while live cells decreased in concentration-, ... distinguishing early apoptosis and necrosis Untreated cells were not stained with Annexin V and PI, suggesting that most of them were intact live cells NNK induced significant apoptosis in concentration-...
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Báo cáo khoa học: " Synergistic effect of ERK inhibition on tetrandrine-induced apoptosis in A549 human lung carcinoma cells" docx
... of ERK inhibition on tetrandrine -induced apoptosis in A549 human lung carcinoma cells 25 Fig Flowcytometric detection of apoptosis of A549 cells treated with tetrandrine (TET) Cells were incubated ... examined TET treatment induced apoptosis and resulted in a decrease in Akt and ERK expression PI3K inhibition had no clear synergistic effect on tetradrine -induced apoptosis, however, ERK inhibition ... 10-14% in the USA [11] Therefore, it is clear that Synergistic effect of ERK inhibition on tetrandrine -induced apoptosis in A549 human lung carcinoma cells Fig The effects of a PI3K/Akt inhibitor...
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Báo cáo y học: "Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells" pps
... conditions in mouse embryonic stem cells Methods Mouse embryonic stem cell culture The mouse embryonic stem (MES) cells (CCE-24) were routinely grown on 0.1% gelatin-coated dishes in Dubecco’s ... hindering the access for the long form protein, thus having an advantage to be evolutionarily selected and maintained Since the truncated protein itself does not contain the c-terminal cysteines, ... predicted open reading frame Thin lines indicate exon-exon junctions A The thick line points to the junction of exon and skipping exon in the truncated form but is included in the long form of...
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báo cáo khoa học: "Basic Mechanisms of Arsenic Trioxide (ATO)Induced Apoptosis in Human Leukemia (HL-60) Cells" potx
... expression of annexin positive cells in ATO-treated cells compared to the control Annexin-V is a specific phosphatidylserine-binding protein used to detect apoptotic cells by providing an assessment ... seen in Figure 2, there was a gradual increase in annexin V positive cells (apoptotic cells) in ATO-treated cells compared to the control However, a marked and dose-dependent decrease in annexin ... granular contents into the surrounding extracellular space, with subsequent inflammation [30-32] To gain insight into the mechanism of arsenic trioxide (ATO) -induced apoptosis, we examined the modulation...
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báo cáo khoa học: "Mitochondrial and endoplasmic reticulum stress pathways cooperate in zearalenone-induced apoptosis of human leukemic cells" doc
... There was G1 arrest in HL-60 cells treated with 50 μg/ml ZEA (Figure 2C) and in U937 cells with 16 μg/ml (Figure 2D) Mitochondria involvement in ZEA -induced HL-60 and U937 cell apoptosis The reduction ... mitochondrial Ca 2+ levels have been found in ER stressed cells [36] As indicated above, apoptosis of leukemic cells induced by ZEA also involved ER stress, Ca2+ levels in both mitochondria and cytosol ... volume and shown in this table as folds of increase or decrease (-) Fusarium [38,39] In the present study, ZEA induced apoptosis in human leukemic HL-60 and U937 cell lines, but less in PBMCs, as...
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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt
... of stem cells into the osteogenic lineage in vitro J Bone Miner Res 2004, 19:1379-1394 Page of 38 Trounson A: Human embryonic stem cells: mother of all cell and tissue types Reprod Biomed Online ... cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure 1) Generally, the mineralization ... units up to 0.4 mm in diameter was established Feeder-independent murine embryonic stem cells (ESCs) were kindly provided by K Pfeffer (Institute for Microbiology, Heinrich Heine University of...
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báo cáo khoa học:" Induction of osteogenic markers in differentially treated cultures of embryonic stem cells" ppt
... sources including autologous cells as well as allogenic and xenogenic cells [13-16] There are some reports that use totipotential embryonic stem cells in tissue engineering of bone [17,18] Embryonic ... BMP-2 Shown are von Kossa stainings with arrows pointing to the deposition of calcium phosphate-containing minerals that stained in black Figure LIF, stem cell PCR showing amplification of the without ... The increase in reaction products during PCR was monitored by measuring the increase in fluorescence intensity caused by the binding of SYBR green to doublestranded DNA that accumulated during...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... Foundation, and Parkinson's Action Network, as well as the Cancer Research and Prevention Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent ... funding of research involving the more than 60 existing stem cell lines, but will not sanction or encourage destruction of additional human embryos Indeed, the embryos from which the existing ... Fischbach GD, Fischbach RL: Stem cells: science, policy, and ethics J Clin Invest 2004, 114:1364-1370 Donovan PJ, Gearhart J: The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97...
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Báo cáo y học: "The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells" pot
... by genotyping The ES cell lines were maintained in the undifferentiated state by culturing on gelatinized plates in KO-DMEM (Invitrogen, Carlsbad, CA, USA) supplemented with leukemia inhibitory ... was seen in Dicer KO cells (17 compared with in matched wild-type cells) Increased minor and major satellite transcripts were also seen in Eed deficient ES cells but not in other mutant lines that ... chromatin components In this regard, compensatory chromatin modifications have been previously described, including an increase in H3K27me3 levels in Suv39h1/h2 deficient ES cells [25] Minor and...
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Báo cáo y học: "A recessive genetic screen for host factors required for retroviral infection in a library of insertionally mutated Blm-deficient embryonic stem cells" doc
... system in generating genome-wide homozygous mutations to facilitate recessive genetic screens in vitro by identifying mCat-1 as a critical gene in ES cells that is required for retroviral infection ... high infection efficiency must be achieved so that every single infectable cell in the culture has at least one infection event In practice, the need to infect every cell in a culture of 109 cells ... on ES cells after a single round of infection with 0.1 ml viral supernatant are illustrated by staining puromycin-resistant colonies in 24-well plates Figure (see following page) Super-infection...
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Dissecting transcriptional network in mouse embryonic stem cells
... undifferentiated cells and differentiated cells of multiple lineages EC cells could be maintained indefinitely with mitotically inactivated embryonic fibroblast in vitro, and is able to give rise to cells ... state of ES cells for in vitro feeder free culture, in vivo ICM cells are able to develop into ES cells in the absence of LIF signaling, indicating that alternative pathways might be involved Recent ... These cells were initially maintained in culture as self-renewal and pluripotent cell lines in either EC cell-conditioned medium (Martin, 1981), or in a co-culture system in which cells were grown...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... effective in vitro system to study complex events underlying human nervous system development (Ben-Nun and Benvenisty, 2006; Dvash et al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells ... forebrain, midbrain, hindbrain and the spinal cord This figure was reproduced from Epstein et al., 1999 2.4 SHH and neural development During the initial phase of neural induction, the ectoderm is induced...
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