tổng quan về chi nhánh tư vấn thiết kế và xây dựng tổng công ty cổ phần xây dựng công nghiệp việt nam

Cloning, expression and characterization of oxysterol binding protein homologue 7 (OSH7) in yeast saccharomyces cerevisiae

Ngày tải lên : 03/10/2015, 20:57

... GST-Osh7cc interacts in vitro 79 15 In vivo activity of OSH7-GFP 80 16 Osh7p-GFP shows different localization in wild-type and vps4∆ cells 81 17 Specificity of anti-Osh7p polyclonal antibody 82 18 Osh7p ... manufacture of steroid hormones and bile acids The very property that makes it useful in cell membrane, namely its absolute insolubility in water, also makes it lethal The amount of cholesterol ... reductase activity and levels • Regulation of excess intracellular free cholesterol through the activity of acyl-CoA:cholesterol acyltransferase, ACAT • Regulation of low density lipoprotein (LDL)...

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KRONE - What is Patch by Exception

Ngày tải lên : 26/10/2013, 19:15

... reductions common mounting can be achieved using existing LSA-PLUS mounting, and does not require expensive "19 inch" accommodation such as racks and cabinets When patching is required, it can be carried ... calls can be easily administered by the IT Manager in a matter of seconds by using the patching facility If these changes are to become permanent the installer can hardwire them at the next monthly ... point method of termination which outperforms the competition in ease of installation, quality, and durability of the connection • • • Insulation clamping ribs hold the wire firmly in position and...

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Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf

Ngày tải lên : 15/02/2014, 01:20

... Kohama K, Fukushima T, Miyata S, Uchiyama S, Uchinokura S, Shimomura T et al (2005) Hepatocyte growth factor activator inhibitor type (HAI-1) is required for branching morphogenesis in the chorioallantoic ... in the mouse embryo [8] Hepsin has an ability to convert pro-HGF to the active form with an activity comparable with HGFA The HGF-converting activity is inhibited by HAI-1 [9,10] The protease ... TMPRSS4 expressed in this system did not show protease activity Thus, other TTSPs that did show activity were tested for the inhibitory activity of HAI-1 using the ﬁrst Kunitz domain of HAI-1 (HAI-1–K1)...

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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx

Ngày tải lên : 18/02/2014, 04:20

... 11224 13 Futai E, Kubo T, Sorimachi H, Suzuki K & Maeda T (2001) Molecular cloning of PalBH, a mammalian homologue of the Aspergillus atypical calpain PalB Biochim Biophys Acta 1517, 316–319 ... system was originally identiﬁed as machinery contributing to multivesicular endosome (multivesicular body) formation in the endocytic pathway [24,25] ESCRT machinery has been proposed to have additional ... which were previously shown to lose the ability to bind to the VPS4 MIT domain [32] As shown in Fig (bottom panel), the immunoreactive band for wild-type FLAG-IST1 (WT) was clearly detected, but...

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Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efﬁcient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt

Ngày tải lên : 18/02/2014, 12:20

... tetrameric forms [14,17,21–23] As the adenosine moiety does not show electron density in any of the human TK1 structures, it may be that the adenosine moiety is of no signiﬁcance for inducing the reversible ... preservation of enzyme activity The fractions were assayed for thymidine kinase activity under standard assay conditions with 100 lm dThd Thymidine kinase assay Thymidine kinase activity was assayed by ... (hTK1) is a regulatory enzyme that can occur in two forms, a dimer with low activity and a tetramer with high activity The conversion between the two forms is reversible and depends on enzyme concentration...

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Tài liệu Báo cáo khoa học: Olfactory receptor signaling is regulated by the post-synaptic density 95, Drosophila discs large, zona-occludens 1 (PDZ) scaffold multi-PDZ domain protein 1 pptx

Ngày tải lên : 18/02/2014, 13:20

... density 95, Drosophila discs large, zonaoccludens (PDZ) domains, has the ability to bind to various molecules in the signal transduction cascade, thereby bringing them into close proximity and ... the cell, avoiding cross-talk with other signaling pathways, in order to ensure the rapidity and speciﬁcity of signaling, remains an unanswered question The idea of the existence of an ‘olfactosome’, ... 5-hydroxytryptamine receptor type 2C [18] Subsequently, many diverse interaction partners of MUPP1 have been characterized, including G-protein coupled c-aminobutyric acid receptor B (GABAB)...

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Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx

Ngày tải lên : 18/02/2014, 18:20

... tested for CDK4 Y17 kinase activity (Fig 1C), and activity was detected in all cell lysates with no activity in the buffer control Interestingly, a higher level of activity was detected in HeLa nuclear ... final purification of approximately 939-fold was achieved Purification by butyl-sepharose chromatography resolved the majority of the Y17 kinase activity as a large peak between fractions 96 and 106, ... kinase activity with a peak in fraction 42 (Fig 2C) A sample of the band was excised and the constituent proteins were analysed by Q-TRAP MS (Applied Biosystems, Foster City, CA, USA) Forty-nine ions...

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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc

Ngày tải lên : 19/02/2014, 00:20

... no effect on the ability of Cytokine production (pgÆmL)1) Treatment IL-1b Vehicle SEC1 PDTC SEC1 + PDTC SN-50 SEC1 + SN-50 Wort SEC1 + Wort HIMO SEC1 + HIMO ETYA SEC1 + ETYA MK 886 SEC1 + MK 886 ... used Speciﬁcity was determined by competition of the nuclear protein obtained from the cells treated with ngÆmL)1 of SEC1 for 24 h The NF-jB-DNA binding activity (lanes 2–8) was quantiﬁed by ... SEC1 induces pyrogenicity via NADPH oxidase 3637 SEC1 induces pyrogenicity via NADPH oxidase C.-L Su et al nuclear translocation (Fig 2C, lanes 5–7) The DNAbinding activity of NF-jB induced by...

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Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf

Ngày tải lên : 19/02/2014, 06:20

... NaCl DNA affinity fraction DNA affinity fraction Mono Q 0.1 M / FT 0.5 M 1.0 M NaCl Mono Q fraction Mono Q fraction Active fraction Input C ppt - sup - Nuclear fraction LD DNA affinity fraction ... 15 Fig Purification of DNA aggregation activity from human cells (A) Scheme for the purification of DNA aggregation activity (B) DNA aggregation activity in chromatographic fractions Nucleoproteins ... references therein] DNA aggregation activity is coupled with EJ activity To further confirm the relationship between protein– DNA aggregation and EJ activity, we fractionated the flow-through of DEAE-Sepharose...

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Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf

Ngày tải lên : 20/02/2014, 02:21

... conﬁrmed the binding speciﬁcity of TFIIIB to the TATATAA sequences Stability of transcriptional complexes on tRNA1Gly -6,7 In order to analyse whether the stability of the tranGly scription complexes ... transcript Gly 10 nucleotides longer than the wild-type tRNA1 -1 without compromising its transcription activity [19], was utilized to differentiate and quantify these transcripts Gly Gly tRNA1 -4 partially ... heparin-resistant complexes on the tRNA1Gly genes (A) The stability of the transcription complexes on the tRNA1Gly genes was tested by their ability to form TFIIIC ⁄ TFIIIB complexes in the presence of...

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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf

Ngày tải lên : 20/02/2014, 11:20

... (C) Relative luciferase activity of 5¢-truncations of fgl2 promoter constructs in endothelial cells Activity of pGl2()1320/+9)Luc is set at 100% (D) Luciferase activity of 3¢-truncation of fgl2 ... reduced promoter activity The mutation at positions )77 to )71 (containing a Sp1 binding site: 5¢-GCCCGCCC-3¢) reduced the promoter activity to 20% of the parental wild-type promoter The mutations ... promoter activity to 40% and 50% of that observed with the wild-type promoter However, mutating the AP1 site (from )50 to )44: 5¢-TGAGTCAG-3¢) did not have any impact on fgl2 promoter activity in vascular...

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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf

Ngày tải lên : 21/02/2014, 01:21

... activityb (unitsÆmetal ion)1) (100) (46) (0) (151) a Activity of SOD as measured by the xanthine-xanthine oxidase assay with percentage activity relative to wild type in parentheses Activity of ... introduce an iron-supported SOD activity which did not exist in the wild-type enzyme, though this was only 6% of manganese-supported activity in the wild type [32] Here we present data for FeSOD ... selectivity were not apparently related to the activity of the mutant enzymes (Table 1) Mutants lacking a glutamine residue showed the highest selectivity but either low or no discernible SOD activity...

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Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt

Ngày tải lên : 22/02/2014, 04:20

... but lower affinity to chromatin The lower affinity could not be detected in an assay involving soluble proteins (Fig 2) These results showed that the NM and RS regions have affinity for chromatin ... column in Fig 6A) Fifty percent suppression with staurosporine was achieved with as little as nM (data not shown) These results indicate that the increase in the affinity of NK to chromatin ... staurosporine, A3 and K252b (Fig 7A) Fifty percent suppression with staurosporine was achieved with nM (data not shown) These results suggest that the decrease in the affinity of NK and chromatin is an...

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Tài liệu Báo cáo Y học: Progressive apoptosis in chorion laeve trophoblast cells of human fetal membrane tissues during in vitro incubation is suppressed by antioxidative reagents pptx

Ngày tải lên : 22/02/2014, 07:20

... of Mn-SOD and Cu/Zn-SOD, tissue samples (wet weight of 0.2 g) were homogenized with a prechilled Potter-type Teﬂon homogenizer in 0.4 mL lysis buffer (10 mM Tris/HCl pH 7.4, containing 1% SDS, ... and glutathione reductase were also expressed constitutively and showed no quantitative alteration We provisionally named this group of genes group-1 Expression of mRNA for c-Jun, IFN-a, IFN-b, ... the quantiﬁcation of changes in mRNA levels of 23 apoptosis-related genes in chorion laeve tissue during in vitro incubation of human fetal membranes obtained at term Based on the extent of quantitative...

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Tài liệu Báo cáo Y học: Toxicity of substrate-bound amyloid peptides on vascular smooth muscle cells is enhanced by homocysteine docx

Ngày tải lên : 22/02/2014, 07:20

... counted, then averaged and expressed as a percentage of total seeding density per well MTS assay of cell viability Cellular viability was measured using the CellTiter 96 AQueous One Solution Cell Proliferation ... this toxicity was enhanced In the presence of homocysteine, Ab1–40 caused a 40% increase in toxicity over that with homocysteine alone, while Ab1–42 caused a further 50% increase in toxicity (Fig ... neurons [14–17] Although the role of Ab in neuronal toxicity has been extensively studied in recent years, the mechanism of this toxicity is unclear Ab peptides have been shown to be neurotoxic...

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Báo cáo khoa học: Visfatin is induced by peroxisome proliferator-activated receptor gamma in human macrophages pdf

Ngày tải lên : 06/03/2014, 22:21

... differentiation [1], visfatin, is nicotinamide phosphoribosyl transferase (NAMPT) [2,3], a rate-limiting enzyme in the synthesis of NAD+ from nicotinamide Visfatin ⁄ PBEF ⁄ NAMPT is synthesized and secreted ... chemiluminescence ECL detection kit (Amersham) and the intensity of signals was subsequently analyzed by densitometry and quantiﬁed using Quantity One software Measure of visfatin protein secretion by ... protein expression was analyzed by western blotting and relative signal intensities were quantiﬁed using Quantity One Software The results are representative of four independent macrophage preparations...

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Báo cáo khoa học: Autocatalytic processing of procathepsin B is triggered by proenzyme activity doc

Ngày tải lên : 07/03/2014, 03:20

... the initial activity observed during processing belongs to the activity of the cathepsin B zymogen, as detected by a small synthetic substrate and afﬁnity labelling by the activity-based probe 125I-DCG-04 ... procathepsin B is typically 45–50% efﬁcient, a higher inhibitor concentration would completely abolish any catalytic activity of the enzyme, thereby preventing detection of cathepsin B activity All processing ... Autocatalytic processing of procathepsin B whether this activity belonged to the zymogen [14] To address this question, the possible activity of procathepsin B on this substrate was investigated by...

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Báo cáo khoa học: The autophagic response to nutrient deprivation in the hl-1 cardiac myocyte is modulated by Bcl-2 and sarco⁄endoplasmic reticulum calcium stores ppt

Ngày tải lên : 07/03/2014, 09:20

... complexity in the control of autophagy Physiologic and pathophysiologic implications of this relationship to cardiomyocyte function are discussed Results Inhibiting lysosomal activity to quantify ... right) 3186 16 Fig Inhibition of lysosomal activity with the inhibitor cocktail (A) Inhibition of cathepsin B activity by lysosomal inhibitors Activity and intracellular distribution of cathepsin ... autophagic activity during nutrient deprivation The effect of the interaction between Beclin and Bcl2 ⁄ -XL on autophagic activity is unclear Control cells exhibited robust autophagic activity in response...

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Báo cáo khoa học: Expression of the Drosophila melanogaster ATP synthase a subunit gene is regulated by a transcriptional element containing GAF and Adf-1 binding sites pptx

Ngày tải lên : 07/03/2014, 16:20

... had signiﬁcantly lower promoter activity, reaching only 13% of the maximal activity, while the )61/+86 construct directed similar levels of luciferase activity as the pXp2 vector (Fig 3) These ... activity Each DNA element in the )146/ +86 promoter construct (whose luciferase activity was considered as 100%) was mutated and the activity of the mutant constructs was assessed Wild type and ... black boxes The luciferase activity of the vector with no insert was deﬁned as being equal to one Luciferase activity was normalized to the b-galactosidase activity of cotransfected control plasmid...

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Báo cáo khoa học: Androgen receptor function is modulated by the tissue-speciﬁc AR45 variant ppt

Ngày tải lên : 07/03/2014, 16:20

... vector The number of viable cells was measured after days by quantifying the ATP levels (grey bars) In the control experiment empty pSG5 plasmid was used (white bars) The results are a representative ... at the same level (lane 1) Even though the identity of the 45 kDa protein detected in LNCaP cells was not demonstrated due to the unavailability of a speciﬁc antibody, the results showed that ... activity When coexpressing the oncoprotein, b-catenin, a fourfold stimulation was seen (Fig 7A) A similar induction was also seen when using the S33F b-catenin mutant, a form with enhanced stability...

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