... flavocytochrome b 558 , 800 nM p47phox, 450 nM Rac1, 900 nM of truncated p67phox, and 0.2 mM arachidonate The fluorescence emission spectrum of 52 5 nm (excitation wavelength, 4 75 nm) was recorded ... Blood 1996, 87 (5) , 166381 Rotrosen D, Yeung CL, Leto TL, Malech HL, and Kwong CH Cytochrome b 558 : the flavin-binding component of the phagocyte NADPH oxidase Science 1992, 256 (50 62), 1 459 -62 Rotrosen ... flavocytochrome b 558 that had been reconstituted with FAD or FAD analog and phospholipids, 800 nM p47phox, 900 nM p67phox, 450 nM Rac, 10 mM GTPgS, and 200-240 mM arachidonate in a total of 50 ml Three...
... 181,791,321 to 181,826,339 15 NCF4 22 35, 586,991 to 35, 604,004 10 CYBB X 37 ,52 4,264 to 37 ,55 7, 658 13 CYBA 16 87,237,199 to 87,244, 958 RAC2 22 35, 951 , 258 to 35, 970, 251 Positions according to National ... or in the EIRA cohort (rs1804006 [CYBA], rs789183, rs1330 658 1 and rs1330 657 5 [NCF2], and rs13 057 803, rs100 350 1, rs12 158 689, rs961 059 5, rs2072706 and rs2072711 [NCF4]; Additional file 1) Fourteen ... OR 95% CI P All 0.80 0.66 to 0.97 0.02 1.04 0.67 to 1.62 0.86 0.94 0. 75 to 1.17 0 .56 1.01 0.60 to 1.71 0.97 0 .50 0. 35 to 0.73 0.0002 1.20 0 .52 to 2.81 0.67 All 0. 85 0.69 to 1.04 0.12 0.92 0 .56 ...
... polymerase (Invitrogen) was added at 80°C after a 94°C ‘hot start,’ initiating 35 cycles of PCR: 94°C for 45 s, 55 °C for 30 s, and 72°C for 90 s, with final extension at 72°C for 10 PCR products ... interact DQA1* 050 1 CTAATTAGCC 58 2 ± 130 DRA CTGATTGGCC 2480 ± 51 9 DQB consensus CTGATTGGTT 1977 ± 93 DRB consensus CTGATTGGTT 1266 ± 274 Discussion DRB-DR4 CTGATTCGTT 1041 ± 258 All antigen-presenting ... arthritis (JCA): search for a specific autoantibody associated with JCA Ann Rheum Dis 1991, 50 :59 5 -59 8 Szer W, Sierakowska H, Szer IS: Antinuclear antibody profile in juvenile rheumatoid arthritis...
... templates for PCR by using the following thermal cycling parameters: 15 minutes at 95 C, 35 cycles of 45 s at 95 C, 35 s at 55 °C and 50 s at 72°C and finally 72°C for minutes The primers were designed ... digested genomic DNA using the following thermal cycling parameters: 15 minutes at 95 C, 35 cycles of 45 s at 95 C, 45 s at 50 °C and minute at 72°C, and finally 72°C for minutes The sequences of ... 400 241 302 476 CpG sites 31 26 42 25 31 44 A/C (-) rs2297246 A/C (+) rs56270809 A/G (-) rs2838343 A/G (+) rs 558 60816 del/no del (+) rs4 156 30 15 C/G (+) rs 256 78 Individuals studied 38 19 33 36...
... 100%, 50 %, 25% , 12 .5% , and 6. 25% A “C” peak which represented the WT BCR-ABL KD allele could be seen in 50 %, 25% , 12 .5% , 6. 25% , 3.13%, and 0% dilution (100% WT) (Figure 2B) 3.3 Detection for T315I ... T315I peak had a different peak pattern from other common mutations (Y 253 F, Y 253 H, E 255 K, M 351 T, and F 359 V) (data no shown) For direct sequencing, a “T” peak indicated the presence of T315I which ... temperature (Ta) (55 ° to 62°C), MgCl2 concentration (1.0-2 .5 mmol/L), and primer ratios (MT: WT ratio of 8:2, 7:3, 6:4, and 5: 5) Briefly, the optimized condition was performed in a 25- μL mixture of...
... Care 2003, 15( 3):347- 357 54 Lenderking WR, Testa MA, Katzenstein D, Hammer S: Measuring quality of life in early HIV disease: the modular approach Qual Life Res 1997, 6(6) :51 5 -53 0 55 Holzemer ... General Health Self Assessment [54 ] Lenderking et al HRQL/QOL 1997 50 32 Living with HIV Scale [55 ] Holzemer et al HRQL/QOL 1998 HIV Cost and Services Utilization Tool [56 ] Hays et al HRQL/QOL 1998 ... Care 1993, 31(7) :56 9 -58 0 19 Wilson IB, Cleary PD: Linking clinical variables with health-related quality of life A conceptual model of patient outcomes Jama 19 95, 273(1) :59 - 65 20 Finch EP, Brooks...
... in 55 % of Rubinstein–Taybi syndrome patients, leaving the diagnosis in 45% of patients to rest on clinical features only Interestingly, this microdeletion of 16p13.3 was found in a young child ... in 100,000 to 1 25, 000 [2] About 55 % of patients have cytogenetic or molecular abnormalities in the Crebbp or E1A binding protein p300 (Ep300) gene, leaving the diagnosis in 45% of patients to ... retardation syndrome Am J Dis Child 1963, 1 05: 588-608 Hennekam RCM, Stevens CA, Van de Kamp JJ: Etiology and recurrence risk in Rubinstein–Taybi syndrome Am J Med Genet Suppl 1990, 6 :56 -64 3 Hennekem RCM:...
... compliance rate higher than 75% was found in 85% of the children at the third month and in 84% of the children at the sixth month and the major cause of withdrawal (5. 6% of the cases) was the cost ... of sublingual-swallow immunotherapy: an analysis of published studies Clin Exp Allergy 20 05, 35: 5 65- 571 de Groot H, Bijl A: Anaphylactic reaction after the first dose of sublingual immunotherapy ... than a SCIT-treated patient for all out-of-pocket costs (€ 176 vs € 255 ) but paid more for allergen extracts (€ 72 vs € 55 ) The sum of direct and indirect costs gave, over the 3-year treatment,...