type and keyword entries
Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... Coomassie blue showed single bands for the wild -type and mutants D392N and E315M, migrating with an Mr of 63 000 (Fig 4A) In the case of the E315Q mutant, an additional faint band was also seen at an ... trimer and the pentamer compared to the dimer were 34% and 30% for the wildtype, but 66% and 47% for the D392N mutant (Fig 5B) A Oligosaccharide synthesis by chitinase A Protein expression and hydrolytic ... oligomers The maximum yields of (GlcNAc)5 and (GlcNAc)6, synthesized relatively to the hydrolytic product, (GlcNAc)2, were 3% and 9% for the wild -type enzyme and 11% and 12% for the D392N mutant The synthesis...
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Báo cáo khoa học: Molecular dynamics structures of peptide nucleic acidÆDNA hybrid in the wild-type and mutated alleles of Ki-ras proto-oncogene ppt
... strand, and GA(23–24) (Fig 2C) and AT(24–25) (Fig 2D) steps of the PNA strand, flanking the A24 C7 mismatch in PDwt (Scheme 1), and the corresponding AT(6–7) & TC(7–8) steps of the DNA strand ... )1.3 A, ˚ and )10.7°, respectively, for PDwt, and 3.3 A ˚ )4.0 A and )10.6°, respectively, for PDmut Average ˚ widths of minor and major grooves are around 9.5 A ˚ in both PDwt and PDmut and 25 ... cis) (A and B) and (a,e) (gauche+, trans) (C and D) conformations O6¢(G) .N2(G) hydrogen bond (C and D) is shown in dotted line (see also Fig 10 and text) Hydrogens at C2¢, C3¢, C5¢ and C8¢...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... wild -type and His-tagged PSI-G by high-resolution SDS ⁄ PAGE (C) Immunodetection of His and Strep tags in PSI-G constructs and transformed plants were treated separately with thermolysin and trypsin ... slightly smaller, paler and flower slightly later than wild type [5] However, in our growth chambers, DG plants display a less pronounced phenotype In total, c 620 T1 and T2 plants from two separate ... connected by a stromal loop, with the N- and C-termini in the thylakoid lumen In order to test this topology prediction, wildtype and tagged PSI-G were transcribed and translated in vitro, then incubated...
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Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot
... isolated and NMR chemical shift data were determined for core-oligosaccharides of the R1 and R2 core-types [8] whereas the chemical structure of the inner core-region of the E coli R4 core -type was ... bell function and zero-filled in F1, 1024 data points NOESY was recorded phase-sensitive using the Bruker NOESYPRTP pulse program Four-thousand and ninety-six data points in F2 and 512 experiments ... p.p.m in F1 and 3.5 p.p.m in F2 Two-hundred and fifty-six experiments were recorded at 2048 data points per increment and a TOCSY mixing time of 67 ms For HMBC, F1 was enlarged to 180 p.p.m and the...
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Báo cáo Y học: Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli pptx
... sarcosine or sarcosine analogues (monomeric and tetrameric sarcosine oxidases and sarcosine and dimethylglycine dehydrogenases), on D-amino acids (DAAO and DASPO) and on pipecolic acid (PIPOX) (see Table ... codon is shown in bold and the ATG codon corresponding to the Met1 of the wild -type GO in italic chloramphenicol (100 lgÆmL)1 and 34 lgÆmL)1 final concentration, respectively) and induced at D600 ... (R302, see Fig 2C) On the other hand, and according to the sequence comparison, M261 in GO appears to be located at the position occupied by Y238 and H269 in DAAO and MSOX, respectively This second...
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Báo cáo khoa học: "Towards an Iterative Reinforcement Approach for Simultaneous Document Summarization and Keyword Extraction" doc
... corpus and p(ti) and p(tj) are respectively the probabilities of the occurrences of ti and tj, i.e count(ti)/N and count(tj)/N, where count(ti) and count(tj) are the frequencies of ti and tj ... information 2.2 Keyword Extraction Keyword (or keyphrase) extraction usually involves assigning a saliency score to each candidate keyword by considering various features Krulwich and Burkey (1996) ... as the n-gram, word sequences and word pairs between the candidate summary and the reference summary ROUGE toolkit reported separate scores for 1, 2, and 4-gram, and also for longest common subsequence...
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Báo cáo khoa học: Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334fiGly mutant enzymes pot
... phosphate in wild -type CcStP and the H334G mutant using 31P-NMR (A) Spectra of wild -type CcStP and the H334G mutant acquired at pH 7.25, and with the number of recorded scans and resulting signal-to-noise ... wild -type CcStP and the H334G mutant using STD NMR Values are relative STD effects of Glc1P bound to wild -type CcStP (Aa) and the H334G mutant (Ab) as well as X1P bound to wild -type CcStP (Ba) and ... annealing and elongation time of and an annealing temperature of 55 °C We used the following pairs of flanking and mutagenic primers, where Eco91I and XagI restriction sites are underlined and the...
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Báo cáo khoa học: An intermediate step in the evolution of ATPases ) the F1F0-ATPase from Acetobacterium woodii contains F-type and V-type rotor subunits and is capable of ATP synthesis Michael Fritz and Volker Muller ¨ docx
... A-ATPase catalytic and proteolipid subunits J Mol Evol 46, 703–715 Cross RL & Muller V (2004) The evolution of A-, F-, ¨ and V -type ATP synthases and ATPases: reversals in function and changes in ... this argument was difficult to understand, as the genes encoding subunits a and b were embedded in the atp operons of M thermoacetica and A woodii [22,29], and one would have to envision a mechanism ... motor Most important, both types of c subunits were present This made it possible to readdress the longstanding question of whether an 3422 ATPase with F0- and V0 -type rotor subunits is able...
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Báo cáo khoa học: Alternative substrates for wild-type and L109A E. coli CTP synthases Kinetic evidence for a constricted ammonia tunnel doc
... 0.50, 1.0, 5.0, and 10.0 mM), Gln-OH (1.0, 3.0, 5.0, 7.0, and 10 mM), or GlnNH2 (3.0, 7.0, 10.0, 15.0, and 20.0 mM) CTPS concentrations ranged between and 56 lgặmL)1 for wild -type and 554 lgặmL)1 ... amidotransferases can utilize NH2OH and NH2NH2 in place of NH3 [4244] Both NH2NH2 and NH2OH (and its derivatives NH2OCH3 and CH3NHOH) Exogenous NH3 and its analogues Exogenous NH3, NH2OH, and NH2NH2 all served ... the wild -type and L109A enzymes discriminate between NH3 and the bulkier substrates in terms of binding (i.e elevated Km values for NH2OH and NH2NH2 relative to NH3 for both wild -type and L109A...
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Báo cáo khoa học: The relationship between thermal stability and pH optimum studied with wild-type and mutant Trichoderma reesei cellobiohydrolase Cel7A ppt
... reesei wild -type Cel7A and pH mutant E223S/A224H/L225V/T226A/D262G at pH 5.8 and pH 8.0 and 25 °C Spectra of the wild -type Cel7A at pH 5.8 (d) and pH 8.0 (s), and the pH mutant at pH 5.8 (j) and pH ... phosphate buffer at pH 5.8 and 8.0 Materials and methods Results and discussion Protein production and purification The purification of wild -type Cel7A enzyme and the E217Q and E223S/A224H/L225V/T226A/D262G ... (Fig 5) as compared with the wild -type enzyme A broad and much more complex transition is observed at pH 8.0 (Fig 5B) for both the wild -type and the mutant protein, and a single transition unfolding...
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Báo cáo khoa học: "Aspectual Type and Temporal Relation Classification" doc
... specific and would need adaptation Aspectual Type B focused on the temporal relation between events and the document’s creation time, which is also annotated in TimeML (not shown in that Figure); and ... (NaiveBayes) and S1 (SMO) For task B the best one was P4 (KStar) For task C, the best indicators were P3 (J48), A1 and P3 (JRip), C1 (KStar), A1 (NaiveBayes) and P2 (SMO) Each of the indicators S2, P1 and ... coercion (Moens and Steedman, 1988; de Swart, 1998; de Swart, 2000) Many linguistic contexts pose constraints on aspectual type This does not mean, however, that clashes of aspectual type cause ungrammaticality...
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Tổng quan về các thiết bị trong ngành dầu khí - AN INTRODUCTION TO RIG TYPE AND BASIC DRILL STRING COMPONENTS
... khoan • Semisubmersible Rigs - Giàn khoan nửa nửa chìm • Drill Ships - Tàu khoan LAND RIGS • Most common type of rigs and used to drill wells at onshore locations • Là dạng loại giàn khoan thông dụng ... Chủ đạo bàn rôtơ (Explain the purpose and operation of a kelly and rotary table system) • Biết chức hoạt động hệ thống truyền động đỉnh Explain the purpose and operation of a top drive system) ... Drum Brakes System (Drawwork Braking Sys) − Brake Band: Má phanh − Drum: Tang tời − Brake Level: Tay phanh • Drawworks Disk Brake System − Caliper and Pad Assemblies − Disks − Disk Brakes are More...
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Báo cáo sinh học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strain" pot
... Magnifications, A: 35000; B: 15000; C and D: 90000 In this study, we investigated the interactions between bovine cells (cell lines and PBMC) and MYXV wild type (T1) strain or vaccinal (SG33) strain ... analyses JLD, AA and JG performed electron microscopy studies GF contributed to PBMC infection studies GF and SB coordinated the research BP and SB wrote the manuscript All authors read and approved ... Virus Diseases of Animals, Island Riems), BT cells (ATCC CRL-1390) and MDBK cells (ATCC CCL-22) Each cell line was infected at a multiplicity of infection (m.o.i.) of and cultured for 72 h Then,...
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báo cáo hóa học: " Identifying type and determinants of missing items in quality of life questionnaires: Application to the SF-36 French version of the 2003 Decennial Health Survey" pot
... between 2% and 7% The type of missingness was missing at random for 18 items (items PF1, RP1, RP3, BP2, GH1, GH4, RE2 and all items of VT, SF and MH subscales) and missing not at random for the ... Perneger and Burnand with the SF-12 [4] and of Vercherin et al with the SF-36 [8], that older age, female sex, and to a lesser extent low education and low economic status (blue collar workers and ... questionnaire is short and quick to administer (5-10 min) and welladapted for studies in general populations Strategy for identification of type and determinants of missingness The type of missingness...
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Báo cáo hóa học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains" potx
... Magnifications, A: 35000; B: 15000; C and D: 90000 In this study, we investigated the interactions between bovine cells (cell lines and PBMC) and MYXV wild type (T1) strain or vaccinal (SG33) strain ... analyses JLD, AA and JG performed electron microscopy studies GF contributed to PBMC infection studies GF and SB coordinated the research BP and SB wrote the manuscript All authors read and approved ... Virus Diseases of Animals, Island Riems), BT cells (ATCC CRL-1390) and MDBK cells (ATCC CCL-22) Each cell line was infected at a multiplicity of infection (m.o.i.) of and cultured for 72 h Then,...
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Báo cáo hóa học: " Research Article Normality Criteria of Lahiri’s Type and Their Applications" pdf
... then F is a normal family In 2009, Charak and Rieppo generalized Theorem C and obtained two normality criteria of Lahiri’s type Journal of Inequalities and Applications Theorem D Let F be a family ... Inequalities and Applications 13 Since then, many results related to this conjecture have been obtained We refer the reader to Bruck 23 , Gundersen and Yang 24 , Yang 25 , Chen and Shon 26 , Li and Gao ... function n ≥ be an integer, and let F share CM, then F F , and f assumes the form cez/n , f z f n If F and F 5.6 where c is a nonzero constant Lu et al 30 improves Theorem H and obtained the following...
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Essentials of control techniques and Keyword Stats_2 pptx
... reversed or turned off A and D on with B and C off will drive the motor one way while B and C on with A and D off will drive it the other way By switching B and D on while A and C are off, braking ... stand in the shower, apply proportional control with a high gain If the water is too hot, turn the mixer handle to “cold.” After a second or two the cold water will hit you and you turn the handle ... track This type of sensor has the advantage of extreme simplicity and is at the heart of the “rolling ball” computer mouse It has the disadvantage that the sensing is of relative motion and there...
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Essentials of control techniques and Keyword Stats_3 pot
... compensator and of the combined system are shown in Figures 7.5 and 7.6 7.11 Second Order Responses So far we have considered only real poles and zeros They can also come in complex conjugate pairs, and ... cos(0) = 1 and sin(0) = 0 and the expression follows 91239.indb 78 10/12/09 1:42:24 PM Frequency Domain Methods ◾ 79 7.3 Complex Amplitudes Equation 7.1 allow us to exchange the ungainly sine and ... ) So we can express a mixture of sines and cosines with a simple complex number This number represents both amplitude and phase On the strict understanding that we take the real part of any...
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Essentials of control techniques and Keyword Stats_4 pptx
... that: x = Ax + Bu so x − Ax = Bu and multiplying through by e–At we have e − At x − e − At Ax = e − At Bu and just as in the scalar case, the left-hand side can be expressed as the derivative ... Discrete Time Systems and Computer Control ◾ 101 where M and N are constant matrices once we have given a value to T From this it follows that x (t + T ) = Mx (t ) + Nu(t ) and when we write nT ... State Equations and Feedback As long as there is a risk of confusion between the matrices of the discrete state equations and those of the continuous ones, we will use the notation M and N (Some...
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Essentials of control techniques and Keyword Stats_5 docx
... c=0; d=0; and experiment with values of e and f to get a “good” response (Remember that any changes will only take effect when you “stop” and “run” again.) Experiments show that e and f can be ... error is now (command-x), so we can demand an angle of tilt tiltdem = c*(command-x)-d*vest; which we then constrain with if(tiltdem>.1){tiltdem=.1;} if(tiltdem
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