tunel assay in a549 cells
Báo cáo khóa học: ICAM-1 expression is highly NF-jB-dependent in A549 cells No role for ERK and p38 MAPK docx
... in pulmonary epithelial cells is currently lacking As NF-jB is considered to be a candidate for therapeutic intervention in airway in ammation, we have investigated the role of NF-jB in the induction ... NF-jB binding site (bold text) upstream of a minimal thymidine kinase promoter driving a luciferase gene Confluent cells in 24-well plates were changed to serum-free medium and treated as indicated ... effectiveness of this construct in repressing both NF-jB DNA binding and transcriptional activity in A549 cells [19] Both TNFa and IL-1b produced a robust increase in reporter activity, measured...
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Báo cáo khoa học: The chromosomal protein HMGN2 mediates lipopolysaccharide-induced expression of b-defensins in A549 cells potx
... Interleukin 10 Interleukin Interleukin (interferon, beta 2) Interleukin 16 (lymphocyte chemoattractant factor) Interleukin 1, beta Mitogen-activated protein kinase 14 Mitogen-activated protein ... Mitogen-activated protein kinase Rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant) Rho-associated, coiled-coil containing protein kinase Ras-related C3 botulinum toxin substrate ... (GTP binding protein, 25kda) Wiskott–Aldrich syndrome-like Wiskott–Aldrich syndrome protein interacting protein v-raf murine sarcoma viral oncogene homolog B1 Mitogen-activated protein kinase kinase...
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Báo cáo khoa học: "Cellular uptake of magnetic nanoparticle is mediated through energy-dependent endocytosis in A549 cells" pptx
... gnieb retfA noitanigavni enarbmem amsalp fo noitaitini eht yb derrucco ekatpu fo gninnigeb ehT )B & A4 giF( semosodne eht hguorht nekatpu yllaitini erew selcitraponaN demrofrep saw sisylana MET ... llehs acilis a nihtiw ,)ASU ,hcirdlA-amgiS ;CTIR( etanaycoihtosi B enimadohr ,eyd ecnecseroulf cinagro gniniatnoc selcitraponan citengam elbitapmocoiB s)CTIR(2OiS@PNM elbitapmocoib fo noitaraperP ... egami tnereffid yb nwohs ylraelc saw erutcurts llehs -eroc ,)egami tresni( egami edutingam hgih eht nI egami edutitingam wol a gnisu denimreted saw selcitraponan eht fo noitubirtsid ezis worran eht...
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Báo cáo khoa học: " Synergistic effect of ERK inhibition on tetrandrine-induced apoptosis in A549 human lung carcinoma cells" docx
... apoptotic cells was determined by staining cells with annexin V-FITC and propidium iodide (PI) The annexin V-FITC apoptosis detection kit was purchased from Calbiochem (Canada) After incubation, cells ... apoptotic cells from the time-dependent study *p ＜ 0.05, **p ＜ 0.01 Fig The effect of tetrandrine on the proliferation of A549 cells The viability of A549 cells was measured using the MTT assay The cells ... apoptotic cells in the inhibitor study Data are presented as mean ± SE of independent experiments **p ＜ 0.01 Fig The effect of tetrandrine (TET) on ERK activation in A549 cells The cells were...
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báo cáo khoa học: "Osthole induces G2/M arrest and apoptosis in lung cancer A549 cells by modulating PI3K/Akt pathway" pptx
... procured locally Cell line and culture conditions The human lung cancer cell line A549 was obtained from the China Center for Type Culture Collection (Wuhan, China) and maintained in RPMI-1640 supplemented ... penicillin, and 100 μg/ ml streptomycin at 37°C in a humidified atmosphere of 5% CO2 MTT Assay Cell proliferation was measured using the MTT assay A549 cells were plated at a density of × 10 4cells ... apoptosis by targeting the proteins of Bcl-2 family and the ratio of Bax/Bcl-2 played a critical role in determining whether cells will undergo apoptosis [31,32] In our study, by examining the effect...
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Báo cáo y học: "Early Induction of apoptosis with tobacco smoke and related products in A549 lung epithelial cells in vitro" potx
... others, in experiments with other cell lines [13,14] In order to ensure that the experiments were in fact measuring apoptosis, we also assessed apoptosis by TUNEL staining The finding that following ... 0.05) increase in the number of apoptotic cells (Figures 2a and 2b) While treatment with Taxol also caused a significant (p < 0.05) increase in the number of "indeterminate" cells Interestingly, ... damage induced after tobacco smoke exposure TUNEL staining was used to examine DNA damage in A549 cells 24 and 48 hours after treatment (Figures 3a and 3b) Since the number of detached cells varied...
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Báo cáo y học: "Host gene expression profiling in influenza A virus-infected lung epithelial (A549) cells: a comparative analysis between highly pathogenic and modified H5N1 viruses" ppt
... infection in A549 cells which are not activated in response to RG modified H5N1 virus infection Influenza virus infection results in activation of various signaling events in the host cells In ... Genes involved in chemokine & cytokine mediated signaling in highly-pathogenic and RG modified H5N1 infected A549 cell lines (16 h post infection time point) Red arrow indicates expression in highly-pathogenic ... containing 10% fetal calf serum, 100units/ml penicillin, 100 units/ml streptomycin in tissue culture flasks (Corning, USA) at 37°C in a CO2 incubator Virus infection Monolayers of A549 cells...
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Báo cáo y học: " Differential replication of avian influenza H9N2 viruses in human alveolar epithelial A549 cells" doc
... nucleoprotein (NP) and matrix protein (M1) in the infected cells was examined by using immunofluorescent staining and Western analysis, respectively Positive staining of the NP protein was found in both ... both H9N2-infected and H1N1-infected A549 cells at 24 h post-infection (Fig 2A) Interestingly, strong M1 protein expression was observed in cells infected with H9N2/G1 at h and 24 h post-infection ... protein and acid polymerase protein (PA) in virus-infected A549 cells at h, h and 24 h post-infection by using quantitative RT-PCR assay [11] The coding regions of the respective viral genes in...
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Báo cáo y học: " Legionella pneumophila infection induces programmed cell death, caspase activation, and release of high-mobility group box 1 protein in A549 alveolar epithelial cells: inhibition by methyl prednisolone" pot
... replicated in flow-cytometry analyses of infected A549 cells (Fig 6a and 6b) 40 Figure growth of L pneumophila in A549 cells Intracellular Intracellular growth of L pneumophila in A549 cells A549 cells ... mitomycin C mitomycin C mitomycin C/m-P pan-actin Figure detectedactivity in L blotting Caspase8by western pneumophila-infected A549 cells Caspase activity in L pneumophila-infected A549 cells ... nuclear DNA fragmentation in infected A549 cells as a possible mechanism of L pneumophilainduced cytotoxicity by TUNEL staining A549 cells infected with the virulent strain of L pneumophila, AA100jm,...
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Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
... Medicine 57 Reagents F12 culture medium (Hangzhou Jino Biology Co., Ltd China), fetal bovine serum (FBS), ampicillin, penicillin and streptomycin (Shanghai Bio-engineering Co., Ltd China), brain ... O2, 85% N2, 10% CO2) with continuous shaking E coli were maintained in the LB liquid medium (containing 100 µg/ml ampicillin) at 37°C for 12 h with continuous shaking Preparation of Hp and E.coli ... Ltd USA) were used in the present study Cells and Hp Culture AGS cells were grown in the F12 culture medium containing 10% FBS, 100 U/ml penicillin and 100 µg/ml streptomycin in a humidified atmosphere...
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... almost no increase in the phosphorylated JNKs protein level in the cell line Twenty-four-hour exposure to hypoxia resulted in a Int J Med Sci 2007, 42 marked increase (1.8-fold of control) in the ... Morpho/AS was examined using a fluorescent microscope Fluorescence was mainly observed in cytosol areas in transfected cells FITC-labeled Bag-1 Morpho/AS was transfected into almost all JAR cells by EPEI-mediated ... and methods Cell line and cell cultures The human choricarcinoma cell line JAR was obtained from American Type Culture Collection (Manassas, VA, USA) Cells were maintained in RPMI 1640 medium...
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Báo cáo y học: " Low concentration of ethanol induce apoptosis in HepG2 cells: role of various signal transduction pathways"
... after its initial effect on protein kinases phosphorylation The most strikingly and novel finding obtained hereby was the strong inhibition of the GPCR signaling pathway induced mainly by increased ... proteins Oncogene 1996;12:1-9 18 Hoek JB, Pastorino JG Cellular signaling mechanisms in alcohol-induced liver damage Semin Liver Dis 2004;24:257-72 19 Aroor AR, Shukla SD MAP kinase signaling in ... of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 1976;72:248-54 24 Pelech S, Sutter C, Zhang H Kinetworks protein kinase multiblot analysis Methods...
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Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"
... G Elevated c-Jun expression in acute myeloid leukemias inhibits C/EBPalpha DNA binding via leucine zipper domain interaction Oncogene 2003;22(30):4760-4 Staber PB, Linkesch W, Zauner D, Beham-Schmid ... exon 10 and adjacent intronic sequences we could for the first time identify a new single nucleotide polymorphism (SNP) in the intron sequence flanking the 3’ end of exon 10 in out of 35 (20%) ... approximately 101 bp and 444 bp in size in samples either containing the heterozygous C/T or homozygous T/T SNP while these fragments could not be detected in samples lacking the SNP and therefore...
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Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
... involved in claudin-1 expression and localization 99 Indeed, a study shows that increasing β-catenin increases the expression of claudin-1 in colorectal cancer cells, and that claudin-1 expression ... using bryostatin instead increased the expression of claudin-1 in the tight junction complex, and increased tight junction barrier integrity (9) In melanoma, we have shown that claudin-1 expression ... melanoma cells (4) To determine if the nuclear localization of claudin-1 was related to the increased invasive capacity of melanoma cells, we created a claudin-1 expression vector expressing claudin-1...
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Báo cáo y học: "Transporter Molecules influence the Gene Expression in HeLa Cells"
... (GO:0007166) The basigin protein was shown to be present in vascular endothelium in both in the non-neoplastic region of the brain and in tumor cells (http://amigo.geneontology.org/cgi-bin/amig o/gp-details.cgi?gp=UniProtKB:P35613) ... cannot determine the global players involved in such complex phenotypes Integration of the gene expression profiling with specific modulation of gene expression in relevant signalling pathway ... Software and are described in the following part according the definitions of the Illumina’s (ILL) data as well as of the Illumina’s annotations (table 2) http://www.medsci.org Int J Med Sci 2009, 22...
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Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... variety of cell types, including hepatocytes, to long-chain saturated fatty acids (SFAs) led to increased expression of proinflammatory cytokines, inhibition of insulin signaling, induction of endoplasmic ... Pharmigen, San Diego, CA, USA) binding and PI staining were performed according to [44] Following incubation with 1.0 lgÆmL)1 annexin V and 4.0 lgÆmL)1 PI for 20 min, cells were analyzed by flow cytometry ... results that TAG synthesis was initially hindered following stearate administration and was completely interrupted at longer incubation periods Interestingly, the interruption of TAG synthesis...
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Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc
... expressed GAPDH–citrine (chimeric protein with N-terminal GAPDH and C-terminal citrine) and citrine–GAPDH (chimeric protein with N-terminal citrine and C-terminal GAPDH) were localized in the cytoplasm ... on the length of linkers In the linker containing seven amino acids, a Pro-Pro sequence that is not contained in other linkers is incorporated This less flexible structure may inhibit fluorophores ... obtained when cell extracts containing GAPDH–cerulean (chimeric protein with N-terminal GAPDH and C-terminal cerulean) or cerulean–GAPDH (chimeric protein with N-terminal cerulean and C-terminal...
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Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf
... as inactive proenzymes and latency is maintained by an interaction between a cystein residue in the prodomain and Zn2+ in the active site of the catalytic domain Two major types of endogenous inhibitors ... polymerization took place Cell viability assay To determine the viability of the cells, trypsinized cells were suspended in serum-containing medium In order to remove serum, cells were washed three times ... inhibitors in several cell lines [10] The protein itself has no known enzymatic activity, but binds to distinct intracellular target proteins and regulates specific functions involved in tumour...
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Tài liệu Báo cáo khoa học: Expression of poly(A)-binding protein is upregulated during recovery from heat shock in HeLa cells doc
... highly specific peptidebinding domain found in poly(A)-binding protein and a HECT ubiquitin ligase EMBO J 23, 272–281 Craig A (1998) Interaction of polyadenylate-binding protein with the elF4G homologue ... to interact with eIF4G at a stoichiometry of more than : It should be noted here that, in addition to the C-terminal domain, PABP1 has another eIF4G-binding domain within its N-terminal RNA-binding ... growth medium containing 20% fetal bovine serum was added Following a total of 12 h of incubation, the medium was replaced with fresh DMEM containing 10% fetal bovine serum Cells were usually...
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Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt
... to in ammatory sites [14,15] and adhesion of polymorphonuclear cells and monocytes to CNS cells [16,17] Schwann cells (SCs) are glia cells found in the PNS In addition to their roles in myelination, ... al LPS increases ICAM-1 expression in Schwann cells of sciatic nerves A B C D Fig LPS induced the expression of ICAM-1 protein in cultured SCs (A) LPS induced ICAM-1 protein expression in a concentration-dependent ... responses in the PNS [31] These cells may function as antigen-presenting cells and activate T cells in vitro in an antigen-specific and MHC-restricted manner [32], especially in the presence of cytokines...
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