transgenic assay system for in vivo mutagenesis

Báo cáo y học: " A remission spectroscopy system for in vivo monitoring of hemoglobin oxygen saturation in murine hepatic sinusoids, in early systemic inflammation" doc

Ngày tải lên : 13/08/2014, 13:20

... reduction in hepatic sinusoidal HbsO2 during the early stages of systemic inflammation In parallel, we detected an increasing NAD(P)H autofluorescence representing an intracellular inadequate ... spectroscopy system allows reliable in vivo detection of liver sinusoidal HbsO2 In a mouse model of early systemic inflammation, we examined whether the detected changes in hepatic HbsO2 correlated with ... groups in this study, the differences in the backscattered light spectra only represent differences in the HbsO2 In the past, we have shown that bilateral hindlimb I/R results in a systemic inflammation...

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Báo cáo y học: " Identification of unique reciprocal and non reciprocal cross packaging relationships between HIV-1, HIV-2 and SIV reveals an efficient SIV/HIV-2 lentiviral vector system with highly favourable features for in vivo testing and clinical usa

Ngày tải lên : 13/08/2014, 09:21

... as a high affinity binding site for Gag The resulting RNAprotein complex is then targeted to the plasma membrane where virion budding takes place Poly A pol ∆ Env RRE Figure packaging constructs ... The dotted line indicates a deletion damaged areas of the brain Such cells have the potential to be useful for the treatment of Parkinson's disease, spinal cord injury and other inflammatory ... coding regions (pSVR∆GP-CMVGFP) and also the generation of a self inactivating (SIN) vector (pSVR∆SIN-CMVGFP) created by additional deletion in the 3' UTR This will be copied into the 5'LTR during...

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Inducible targeted tagging system for localized insertional mutagenesis in arabidopsis thaliana

Ngày tải lên : 16/09/2015, 15:55

... related serine/threonine protein kinases 93 Figure 33: Phylogenetic tree for Serine/Threonine protein kinases 94 Figure 34: GUS staining in L267; an insertion in the sense orientation for At2g30740 ... of GUS staining for L479; an insertion in the sense orientation in At2g30830 80 Figure 27: A schematic representation of GUS staining in L227; an insertion in the sense orientation in At2g30840 ... then introduced into Arabidopsis WS (O) by root transformation This reduces labor involved in generating several independent transgenic lines and since the transposase source is inducible, crossing...

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Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx

Ngày tải lên : 07/03/2014, 21:20

... selenocysteine residue in a C-terminal Sel-tag [18] Der p carrying the Sel-tag had an intact core sequence and maintained allergen-speciﬁc IgE-binding epitopes and the use of a Sel-tag enabled labelling ... inhalation In this study, we tracked inhaled Der p in vivo using the recently developed Sel-tag method in a mouse model for Der p 2-sensitization Based upon our ﬁndings we conclude that the in ammatory ... In vivo tracking of 75 Se-labelled Der p intrinsic biological properties of the allergens may all contribute to their allergenicity [1–4] The intrinsic properties required for evoking an...

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Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc

Ngày tải lên : 17/03/2014, 10:20

... motif Therefore the systematic functional analysis of the six cysteines in yeast Erv1p was the major goal of this paper All three pairs of cysteines in Erv1p are indispensable for in vivo activity ... bacterial dsbB protein in the periplasm also contains two cysteine pairs acting in concert for the transfer of disulﬁdes to dsbA [25] Interestingly, the redox function of dsbB is linked to the bacterial ... cysteines of yeast Erv1p can be grouped into three pairs (Fig 1): one pair near the N-terminus of the protein, the central pair close to the FAD binding motif and two cysteines in the C-terminal...

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Báo cáo hóa học: " Are quantum dots ready for in vivo imaging in human subjects?" docx

Ngày tải lên : 22/06/2014, 18:20

... eventually translate QDs for use in clinical applications such as in vivo imaging in human subjects Modeling studies have revealed that two spectral windows exist for QD imaging in living subjects, one ... the injected dose of all types of QDs tested in these studies was excreted in the feces within 1–2 days In vivo targeted imaging using QDs In order to make QDs more useful for in vivo imaging ... exhibited high affinity integrin avb3 specific binding in cell culture and ex vivo In vivo NIR fluorescence (NIRF) imaging was carried out on athymic nude mice bearing subcutaneous integrin avb3-positive...

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Báo cáo khoa học: " Investigation of tumor hypoxia using a twoenzyme system for in vitro generation of oxygen deficiency" pot

Ngày tải lên : 09/08/2014, 09:20

... GOX/CAT system In particular, the highest level of CA9 and LOX expression was shown at h incubation for the enzymatic system and then decreased, while a continuous increase over time for the incubation ... of the final version VA, GM, UW, CS and SR were involved in data analysis and data interpretation AA, UH, JD, SM and PEH were involved in critically revising the manuscript for important intellectual ... performed for RNA isolated from cells incubated for the same time period (24 h) under normoxic conditions For bioinformatical-analysis a step-wise approach was applied: Weak signals, below the intensity...

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báo cáo khoa học: "BRCAA1 monoclonal antibody conjugated fluorescent magnetic nanoparticles for in vivo targeted magnetofluorescent imaging of gastric cancer" ppsx

Ngày tải lên : 11/08/2014, 00:23

... cancer of mm in diameter and IVIS imaging system and Magnetic Resonance Imaging, investigated the feasibility of as-prepared nanoprobes for non-invasive in vivo targeted dual modal imaging of gastric ... al: In vivo near-infrared fluorescence imaging of integrin ¦Á v ¦Â in an orthotopic glioblastoma model Molecular Imaging and Biology 2006, 8:315-323 21 Sato A, Klaunberg B, Tolwani R: In vivo ... monitored under a non-invasive manner for 12 h by using IVIS fluorescence imaging system By monitoring real-time fluorescence intensity in the whole body, the tumor-targeting character of the...

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Báo cáo khoa học: A simple in vivo assay for measuring the efﬁciency of gene length-dependent processes in yeast mRNA biogenesis doc

Ngày tải lên : 07/03/2014, 12:20

... they are easy to perform [12]; however, those assays are too indirect to obtain solid conclusions [13] In vitro assays are useful for deﬁning the role of a given element in a speciﬁc step of ... Kokubo T (2001) Mutations in the TATA-binding protein, affecting transcriptional activation, show synthetic lethality with the TAF145 gene lacking the TAF N-terminal domain in Saccharomyces cerevisiae ... timeconsuming and not easy to carry out in a high number of samples In addition, the information obtained by nuclear run-on shows the location of active polymerases, but it gives no information...

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Báo cáo khoa học: " Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo" ppsx

Ngày tải lên : 12/08/2014, 04:20

... Fifty goslings were randomly divided into groups In brief, a group of 40 goslings were orally infected with GPV CHV strain, using 0.1 mL of 103 LD50 per gosling Another group of 10 goslings was ... enabling the investigation of viral dynamics [21] The assay can be used to determine the amount of viral DNA in different tissues at various times after infection; this infection data could be interesting ... we could perform the study on the dynamic distribution of GPV in vivo using these methods, because the animals were certificated as GPV-free by qualitative PCR assay before being infected with...

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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"

Ngày tải lên : 25/10/2012, 11:10

... characterized by insulin resistance and insulin secretion deficiency At present, there is no a single medication which treats type diabetes by improving both insulin resistance and insulin secretion ... glycine-rich linker is flexible, which allows the specific engineering of hinge regions into proteins to achieve desired functional motions [17] We used a glycine-rich short peptide including 15 amino ... Protein Assay Kit PEG20000 was used to concentrate the refolded protein Removal of N-terminal His-tag The refolded protein was incubated with enter- 205 okinase (1U enterokinase was added in 0.5mg...

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PERFORMANCE OF A COMBINED CONSTRUCTED WETLAND SYSTEM FOR TREATING VILLAGE SEWAGE IN LAKE DIANCHI VALLEY

Ngày tải lên : 05/09/2013, 08:40

... performance of the system was investigated by monitoring the influent and effluent quality 4-7 times every month The parameters analyzed, using procedures outlined in the Standard Methods (Chinese ... pollutants containing in the stormwater runoff It has important significance to accumulate the design and maintaining experience for the promotion of constructed wetland technology in the Lake Dianchi ... performance of demonstration system treatment of village sewage (2) the performance of demonstration systems when suffering the impulse load of stormwater and its effect on the reducing non-point...

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A NEW CLASSIFICATION SYSTEM FOR URBAN STORMWATER POLLUTANT LOADING: A CASE STUDY IN THE SANTA MONICA BAY AREA

Ngày tải lên : 05/09/2013, 09:08

... classification for stormwater modeling using Bayesian networks, In Proc.7th IWA Conference, Diffuse Pollution and Basin Management, Dublin, Ireland Pearl, J (1988) Probabilistic Reasoning in Intelligent Systems: ... stormwater pollution by identifying the areas that discharge high pollutant loading into receiving waters METHODS Our area of interest was Marina del Rey and its vicinity in the Santa Monica Bay Watershed ... The training data for the networks were collected using a random subset from all classes The total number of training data pixels was 4,000, which corresponds to 15% of total data All input data...

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Fuzzy AHP based decision support system for selecting ERP systems in textile industry by using balanced scorecard

Ngày tải lên : 07/12/2013, 11:41

... Defined? Define the Strategies with Top Management No Yes Are Key Performance Indicators Defined? No Define the Key Performance Indicators Yes Are Human Resources Qualified for Information Systems? ... modules, inventory costs are very high for fast fashion companies, competition is increasing in the industry and the prices are decreasing, the system is becoming more complex, since Baymen’s ... critical for success These ﬁnancial measures are inadequate, however, for guiding and evaluating the journey that information age companies must make to create future value through investment in customers,...

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Diabetes Type 2 - Screening for Diabetes and Circulation System Disease in Obese Children

Ngày tải lên : 16/01/2014, 14:27

... school, in which the greatest putting on weight is viewed The speed of weight problems in women residing in urban centers held steady from 1976 to 2000 In the last three decades adolescents in Finland ... popularity, a minimum of in urban centers School lunches are planned by nutrition experts and frequently include grain, in your area grown veggies, and sauces No sodas are offered in class lunchrooms ... in Australian children rose from five percent in 1994 to eight percent in 2007, based on the Australian Bureau of Statistics Japanese young children in small cities, show an identical trend In...

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Tài liệu Neural Networks and Neural-Fuzzy Approaches in an In-Process Surface Roughness Recognition System for End Milling Operations pptx

Ngày tải lên : 23/01/2014, 01:20

... Iowa (including Winnebago Co in Forest City; Delavan Inc in Des Moines; Sauer-Sundstrand Inc in Ames), point to the feasibility of in- process surface roughness recognition (ISRR) systems for implementation ... the training and testing 16.4.2 ISRR-FN System The structure of the ISRR-FN, as shown in Figure 16.8, consisted of the sensing system, machining parameters, and ISRR-FN In this sensing system, ... ISRR-FN before or during machining The primary objective was to train the fuzzy system by generating fuzzy rules from input–output pairs, and combining these generated and linguistic rules into a...

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Tài liệu Knowledge Management in OSS—an Enterprise Information System for the Telecommunications Industry doc

Ngày tải lên : 24/01/2014, 00:20

... Support systems, Expert Systems with Application, Knowledge-Based Systems, Computers in Industry, Expert Systems, Data & Knowledge Engineering, Advanced Engineering Informatics, Logistic Information ... 23,177^190 (2006) Jiayin Qi et al Syst Res RESEARCH PAPER Data Data Processing: Organizing, storing, calculating, Retrieving, Reporting Information Information Processing: Reforming, Quantification, ... creating, maintaining, employing, sharing and renewing knowledge Knowledge Management Framework in Systems Point of View Viewing knowledge management as a manmade system, the boundary of the system...

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Tài liệu Báo cáo khoa học: "Domain-transcending mappings in a system for metaphorical reasoning" docx

Ngày tải lên : 22/02/2014, 02:20

... physical to the mental domain Incorporation into a reasoning system ATT-Meta (Barnden & Lee 1999) is a preexisting approach and implemented system for metaphorical reasoning The system is rule based ... not making the transfer in the first place Our principle of query-directed reasoning for metaphor understanding strongly supports the use of VNMAs The kind of information VNMAs convey will form ... implemented context system that combines belief reasoning, metaphor-based reasoning and uncertainty handling In P Bouquet, P Brezillon & L Serafini (Eds), Lecture Notes in Artificial Intelligence,...

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Báo cáo " A web-based decision support system for the evaluation and strategic planning using ISO 9000 factors in higher education " pot

Ngày tải lên : 05/03/2014, 14:20

... Web-based DSS is to provide a way for storing, presenting, gathering, sharing, processing, and using information Web-based DSS is also to provide a distributed infrastructure for P.V Hai, Vatcharaporn ... models in decision making for DSS AHP models are structured by decomposing a problem into a hierarchy of elements such as attributes and alternatives influencing a system by incorporating levels: ... details for the prototype of Web-based DSS application Web interface is described in login process steps of the system System and evaluation modules include input steps and AHP algorithm processing...

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Báo cáo khoa học: Measuring enzyme activities under standardized in vivo-like conditions for systems biology pdf

Ngày tải lên : 06/03/2014, 09:22

... enzyme of interest should be investigated in the context of the in vivo- like medium Finally, in vivo, the enzymes are present at much higher concentrations than in typical enzyme assays, in which ... starting point For different organisms or cell types, it will be necessary to develop dedicated assay media We are aware of and ⁄ or involved in such standardization projects for enzyme assays for ... converted per minute per milligram of extracted protein Protein determination was carried out with the bicinchoninic acid kit (BCA Protein Assay Kit; Pierce, Thermo Fisher Scientiﬁc, Rockford, IL,...

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