... HepG2cDNA library (C. Baisezand A. Harduin-Lepers, unpublished data), as the templateand two specific primers For 6I 5¢-CGATGAATTCGTTAACGCTCATCACCATCACCATCACGGGAAATTGGCCATGGGGT-3¢ containing a HpaIsiteandBack6I ... 00Fetuin NeuAca2-3Galb1-3GalNAca1-O-Ser/Thrc0 1.4NeuAca2-3Galb1-3[Neu5Aca2-6]GalNAca1-O-Ser/ThrcNeuAca2-6(3)Galb1-4GlcNAc-RcAsialofetuin Galb1-3GalNAca1-O-Ser/Thr 66 83Galb1-4GlcNAc-RArylglycosides ... the annealing of the two following synthetic oligonucleotides For EGT 5¢-GATCCGCCACCATGACCATCTTATGTTGGCTCGCTCTCCTGAGCACACTCACAGCTGTTAACGCTGACATCA-3¢ and Back EGT 5¢-GATCTGATGTCAGCGTTAACAGCTGTGAGTGTGCTCAGGAGAGCGAGCCAACATAAGATGGTCATGGTGGCG-3¢....
... initiation, 5¢-(C ⁄ A) AA (A ⁄ C)ATG, and a putativepoly (A) addition signal sequence, 5¢-AATAAA [17,18].It encoded a predicted product of 255 amino acidswith a molecular mass of 28.5 kDa, and ... detection reagents (Amersham PharmaciaBiotech, Piscataway, NJ).Animals were fed water and standard rabbit food andmaintained on a 12 h light/dark cycle. Polyclonal antiserumto the peptide was raised ... proliferating cell nuclear antigen 2 (DmPCNA2) and DmPCNA1 in response toDNA-damaging agents. (A) Immunofluorescent analysis of the localization of V5-tagged DmPCNA2 and Flag-tagged DmPCNA1. DmPCNA2...
... Slaves apparentlycarried a 30-day warranty against certain disorders. For example, if a slave was attack ed by epilepsy within one m onth of purchase, the sellerhad to reclaim that slave an d ... remaining tissue is sewn shut. Female circumcision is seen as a way of ensuring chastity and was often practiced as a coming of age ritualand a prerequisite to marriage.HEALING RITUALS, TRADITIONS, ... scope and quality. Part of the problem was undoubtedlylack of systematic knowledge of anatomy, asepsis, anesthesia, and thefailure ofa small tribal unit to provide enough ‘‘clinical material’’...
... Association of Artificial Intelligence.Rada Mihalcea. 2003. The role of non-ambiguouswords in natural language disambiguation. In Pro-ceedings of the Conference on Recent Advancesin Natural Language ... sense-tagged corpus,the TWA Sense Tagged Data Set, manually pro-duced by Rada Mihalcea and Li Yang (Mihalcea,2003), from text drawn from the British NationalCorpus. We calculated a ‘supervised’ ... MachineTranslation, pages 101–112.Rada Mihalcea and Dan I. Moldovan. 1999. An auto-matic method for generating sense tagged corpora.In Proceedings of the 16thConference of the Amer-ican Association...
... sepa-rated, and they stand or fall together.Ron Paul understands that all three parts of this system of liberty have been under grave attack for decades, and that the main problem is the federal ... individual’s natural right to be free of assault and aggression, and of his right to keep the property that he has earned on the free market, and not have it stolen from him by confiscatory taxes ... coinage of the United States.“Without the automatic check ofa gold standard,” wrote Professor William Quirk in the New Republic, “the Nixon and Carter administrations were able, in a remarkably...
... Forward CAAAAGTCTTGGCGGACAAAATGAGGACTTGGTACReverse CATTTTGTCCGCCAAGACTTTTGAATACTT TCAAGTGCQ76G Forward CTTCCCGGAGGAAATGAGGACTTGGTACTTACTGReverse CCTCATTTCCTCCGGGAAGACTTTTGAATA CN77G Forward ... SequenceStandard All ForwardGCTCAGGCGACCATGGGCCATCATCATCReverse CTTGCATGCCCTGCAGGTCGMutagenic L73G Forward GTATTCAAAAGTGGTCCCGGACAAAATGAG GACTTGReverse TCCGGGACCACTTTTGAATACTTTCAAGTGCATATATTTATTP74G ... sequence of the second binding loop of the related family 1 cystatin,cystatin A, differs appreciably from that of cystatin B.Most notably, cystatin A lacks the essential His75 andinstead has a Gly...
... apokatastaesei panta, lego de humin, hoti Aelias aedae aelthe kai oukepegnosan auton all' epoiaesan auto hosa aethelaesan. Kai gegraptai hoti tote sunaekan oi mathaetai, hoti periIoannon tou Baptistou ... eipen autois.]_Matt._ xvii. 11-13.[Greek: Aelias men erchetai apokatastaesei panta, lego de humin hoti Aelias aedae aelthen kai ouk epegnosanauton, alla epoiaesan auto hosa aethelaesan, [outos ... representation of events in the early part of the rising of the Jews under Barcochba; Judith is Judaea, Nebuchadnezzar Trajan;Assyria stands for Syria, Nineveh for Antioch, Arphaxad for a Parthian...
... of aerobically and anaerobically prepared apoFNR. Aerobicallyor anaerobically prepared apoFNR were incubated with GnHCl +iodoacetate and digested with trypsin, and after separation on Sepha-dex ... anaerobic apoFNR consisted of one majorsignal at 28 408 Da after alkylation equivalent to five-fold alkylated apoFNR, and a minor signal of three-fold alkylated FNR (Fig. 1B). Therefore, aerobic andanaerobic ... MALDI-TOF spectra of peptides derived from carboxymeth-ylated aerobic (A) and anaerobic (B) apoFNR (trypsin digest). Thesamples (carboxymethylated tryptic digest of aerobic or anaerobicapoFNR)...
... sixb-strands (bAtobF) and two a- helices (aA and aB)(Fig. 4). The structure of the C378G variant of SAP97PDZ2was practically identical to that of C378Svariant, except for the mutated residue, and ... 890–907) of GluR -A and of twovariant PDZ2 domains in unliganded state at 1.8–2.44 A ˚resolutions.SAP97PDZ2folds to a compact globular domain comprising six b-strandsand two a- helices, a typical ... that trans-genic mice expressing GluR -A variant lacking sevenC-terminal residues display apparently normal synapticplasticity and basal GluR -A localization [10], suggest-ing developmental...
... log as the most up to date ‘‘truth’’ aboutthe state of the data on disk. The main difference is thatdatabase systems do not use the log as the final repositoryfor data: a separate data area is ... purpose.The separate data area of these database systems meansthat they do not need the segment cleaning mechanisms of the Sprite LFS to reclaim log space. The space occupiedby the log in a database system ... The bandwidth of each of the five phases isshown separately. Sprite LFS has a higher write bandwidth andthe same read bandwidth as SunOS with the exception of sequen-tial reading ofa file that...