... His Submarine Boat (1910)• TomSwift and His Electric Rifle (1911)• TomSwift and His Motor-Boat (1910)• TomSwiftinCaptivity (1912)Copyright: Please read the legal notice included in this ... (1910)• TomSwiftin the City of Gold (1912)• TomSwift and His Undersea Search (1920)• TomSwift and His Photo Telephone (1914)• TomSwift and His Electric Locomotive (1922)• TomSwift and ... friend, who is in businesshere, about this Val Jacinto, and if I find him all right we may engagehim "Inquiries next morning brought the information, from the head of arubber exporting firm...
... (1910)• TomSwiftin the City of Gold (1912)• TomSwift and His Undersea Search (1920)• TomSwift and His Photo Telephone (1914)• TomSwift and His Electric Locomotive (1922)• TomSwiftin the ... him ascare." Tom& apos;s monoplane was making no more noise, now, than a soaringbird. He was gliding swiftly toward the earth, and, with the plan in hismind of administering some sort of ... at night?" asked Tom, thinking there wassomething suspicious in that."Because my life might be in danger if I was seen talking to you, andshowing you diamonds in the daytime—especially...
... (1914)• TomSwift and His Electric Locomotive (1922)• TomSwiftin the Land of Wonders (1917)• TomSwift and His Submarine Boat (1910)• TomSwift and His Electric Rifle (1911)• TomSwift and ... for Appleton:• TomSwift and His Motor-Cycle (1910)• TomSwift and His Airship (1910)• TomSwiftin the City of Gold (1912)• TomSwift and His Undersea Search (1920)• TomSwift and His Photo ... trying.""My! That is certainly interesting!" exclaimed Ned, who had been allattention during the telling of the story."And you certainly had a hard time," declared Tom. ...
... (1912)• TomSwift and His Undersea Search (1920)• TomSwift and His Photo Telephone (1914)• TomSwift and His Electric Locomotive (1922)• TomSwiftin the Land of Wonders (1917)• TomSwift ... His Submarine Boat (1910)• TomSwift and His Electric Rifle (1911)• TomSwift and His Motor-Boat (1910)• TomSwiftinCaptivity (1912)Copyright: Please read the legal notice included in this ... themachine work. His falling in with Mr. Swift and his son seemed provid-ential, for Tom and his father were at once interested in the project for10"Yes, and send us one," added Tom, ...
... government became interested in Tom& apos;s craft, the Humming Bird,and, as told in the ninth book of this series, TomSwift and His SkyRacer, they secured some rights in the invention.8weeks, ... haven't thought of going, Tom Swift. "22the Congo River, and then go into the very interior of the heart of thedark continent.As we have described in detail, in the former books of ... and in this car were the cabins, the living, sleeping and eatingapartments, the storerooms and the engine compartment.This last was a marvel of skill, for it contained besides the gas ma-chine,...
... constructing the car.Certain parts of it could be better purchased in the open market in- stead of being manufactured in Mr. Swift& apos;s shop, and thus Tom was ableto get his new invention into ... exclaimed Tom Swift, looking up from a paper he was reading,"I think I can win that prize!""What prize is that?" inquired the aged inventor, gazing away from adrawing of a ... raining."We don't mind getting wet," replied the young inventor. "It's in the in- terests of science.""Maybe it is. You don't mind a wetting, but I mind...
... whom Tom Swift had brought away from captivity with him, was entering the front gate.He stopped to speak to Mr. Swift, Tom& apos;s father, who was setting outsome plants in a flower bed, taking ... and so got ahead of Koku. The next minute, sliding downto earth, Tom and Ned came to a halt, awaiting the oncoming of Koku,who, finding the glaring light full in his face, came to a halt."Why, ... REPAIRSQuickly TomSwift crossed the space between the airship, that was readyfor a flight, and the tree. The man behind it had apparently not seen Tom coming, being so interested in looking at the...
... related in " ;Tom Swift Among the Fire Fighters," he had engaged in the invention of an explos-ive bomb carrying flame- quenching chemicals that would, in time, revo-lutionize fire- fighting ... thrill-ing ones—since, as a youth, he was first introduced to the reader in theinitial volume of this series, entitled " ;Tom Swift and His Motor Cycle."His first experiences as an inventor, ... crooked crowd of financiers considered that Tom Swift had obtained information from the president of the H. & P. A.that might do his employers signal service? Tom Swift had fallen in with many...
... (1922)• TomSwiftin the Land of Wonders (1917)• TomSwift and His Submarine Boat (1910)• TomSwift and His Electric Rifle (1911)• TomSwift and His Motor-Boat (1910)• TomSwiftinCaptivity ... for Appleton:• TomSwift and His Motor-Cycle (1910)• TomSwift and His Airship (1910)• TomSwiftin the City of Gold (1912)• TomSwift and His Undersea Search (1920)• TomSwift and His Electric ... things that happened to him. He had even more wonderful adven-tures in his submarine, and with his electric runabout our hero was in- strumental in saving a bank from ruin by making a trip in...
... ortwo navigating officers of the craft, were gathered in the operating cabinof the M. N. 1."That's water being pumped into the tanks," explained Tom. "We arenow going down. ... is! A company has been formed in Japan forthe purpose of using a new kind of diving bell, invented by an Americ-an, it seems. The inventor claims that in his machine he can go downdeeper than ... young inventor, with a laugh."You keep away from Rad. You'll get to disputing again and interruptme, and I have business on hand. Here, wait a minute. I'll find somethingfor...
... sử dụng avidin (hay streptavidin) – biotin: đây là một cặp ligand có hằng số ái lực cao nhất tìm thấy trong sinh học. Đầu tiên, mẫu dò đƣợc đánh dấu bằng biotin theo hai cách: biotin đƣợc gắn ... với dung dịch Blocking buffer 1X, chất blocking trong dung dịch sẽ khóa các phân tử lai không đặc hiệu lại. Ủ mẫu với dung dịch Anti – Digoxigenin (Anti – DIG) có gắn Alkaline phosphatase ... amplification). phân tử lai đƣợc phát hiện khi cho ligand của nó là avidin (hay streptavidin) vào phản ứng. Avidin (hay streptavidin) trƣớc đó có gắn với một nhóm phát huỳnh quang hay một enzyme...